LOCUS       BBa_M1722               2461 bp    DNA     linear       26-Nov-2024
DEFINITION  Part is based on theory.  Consists of pBAD promoter, hepcidin and 
            GFP
ACCESSION   BBa_M1722
VERSION     BBa_M1722.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1533
                     /label=BBa_M1716
     misc_feature    1542..1571
                     /label=10x-Histidine Tag
     misc_feature    1572..1574
                     /label=Stop
     misc_feature    1575..1577
                     /label=Stop
     misc_feature    1542..1577
                     /label=BBa_K844000
     RBS             1586..1598
                     /label=RBS-3\Weak
     RBS             1592..1595
                     /label=RBS
     misc_feature    1586..1598
                     /label=BBa_B0032
     RBS             1586..1598
                     /label=BBa_B0032
     CDS             1605..2324
                     /label=GFP protein
     CDS             1605..2324
                     /label=BBa_E0040
     stem_loop       2344..2387
                     /label=stem_loop
     misc_feature    2333..2412
                     /label=BBa_B0010
     terminator      2333..2412
                     /label=BBa_B0010
     stem_loop       2428..2447
                     /label=T7 TE
     misc_feature    2454..2454
                     /label=stop
     polyA_site      2448..2461
                     /label=polya
     misc_feature    2421..2461
                     /label=BBa_B0012
     terminator      2421..2461
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga aagttctggc ggcgtgcgtt
     1261 ttcctgctgc tgctggttct gctgcacggt tctccggcgg cgtgctctgc gctgcgtgcg
     1321 caggcgaaca tcggtctgat gccgcgtccg gaaaccggtg cgcagtctca cggtctggaa
     1381 gcggcggcgg gtctgatgcc gcacccggaa atcggtgcgc agtctctgga agttccgctg
     1441 cgtcgttcta aacgtttcaa ctctcacttc ccgatctgct cttactgctg caactgctgc
     1501 cgtaacaaag gttgcggtct gtgctgccgt acctactaga gcatcatcac catcaccacc
     1561 atcatcacca ttaataatac tagagtcaca caggaaagta ctagatgcgt aaaggagaag
     1621 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
     1681 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
     1741 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt
     1801 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga
     1861 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
     1921 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
     1981 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
     2041 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca
     2101 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
     2161 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
     2221 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
     2281 ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag agccaggcat
     2341 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     2401 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     2461 a
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