BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498842 1 PC range1498842 1 1043 1114 annotation1498841 1 Pbad range1498841 1 1080 1210 annotation1498843 1 AraC range1498843 1 1 1079 BBa_M1720 1 BBa_M1720 Optimized Hepcidin + 6x spider silk 2013-04-03T11:00:00Z 2015-05-08T01:13:58Z The part comes from the AA sequence for hepcidin, but has been optimized for E. coli. This part was designed for a class and does not really exist. It is comprised of the part BBa_M1718, which is a combination of Optimized hepcidin and spider silk, and 5 repeats of the part BBa_K844004. false false _768_ 0 16127 9 Not in stock false Optimized for E. coli using NCBI OPTIMIZER. false Richard Decker component2225545 1 BBa_K844004 component2225515 1 BBa_K844004 component2225500 1 BBa_M1718 component2225530 1 BBa_K844004 component2225575 1 BBa_K844004 component2225560 1 BBa_K844004 annotation2225545 1 BBa_K844004 range2225545 1 942 1145 annotation2225560 1 BBa_K844004 range2225560 1 1154 1357 annotation2225515 1 BBa_K844004 range2225515 1 518 721 annotation2225500 1 BBa_M1718 range2225500 1 1 509 annotation2225530 1 BBa_K844004 range2225530 1 730 933 annotation2225575 1 BBa_K844004 range2225575 1 1366 1569 BBa_M1718 1 BBa_M1718 Optimized Hepcidin + 1x spider silk 2013-03-31T11:00:00Z 2015-05-08T01:13:58Z Both protein coding regions are based on original AA sequences, but have been optimized for expression in E. coli. This part is for a class project and does not really exist. The part combines the part BBa_M1716 that codes for the E. coli optimized hepcidin protein with part BBa_K844004, which codes for a single coding region of spider silk protein. false false _768_ 0 16127 9 Not in stock false No considerations of note. false Richard Decker component2219134 1 BBa_K844004 component2219119 1 BBa_M1716 annotation2219119 1 BBa_M1716 range2219119 1 1 297 annotation2219134 1 BBa_K844004 range2219134 1 306 509 BBa_M1727 1 BBa_M1727 Promoter + RBS + Hepcidin + Silk + His-Tag 2013-04-12T11:00:00Z 2015-05-08T01:13:58Z From various sources. This part was designed for a class and is not available. It contains a pBAD promoter, a standard RBS, the coding region for Hepcidin, a 6x repeat of spider silk, and a 10x histidine tag, followed by a double stop codon. false false _768_ 0 16127 9 Not in stock false Hepcidin has been optimized for expression in E. coli false Richard Decker component2231133 1 BBa_M1721 component2231035 1 BBa_B0034 component2231033 1 BBa_I0500 annotation2231133 1 BBa_M1721 range2231133 1 1237 2849 annotation2231033 1 BBa_I0500 range2231033 1 1 1210 annotation2231035 1 BBa_B0034 range2231035 1 1219 1230 BBa_M1716 1 BBa_M1716 Hepcidin Optimized for E. coli 2013-03-31T11:00:00Z 2015-05-08T01:13:58Z NCBI OPTIMIZER using AA sequence from GenBank number ADA68357. ATGAAAGTTCTGGCGGCGTGCGTTTTCCTGCTGCTGCTGGTTCTGCTGCACGGTTCTCCG GCGGCGTGCTCTGCGCTGCGTGCGCAGGCGAACATCGGTCTGATGCCGCGTCCGGAAACC GGTGCGCAGTCTCACGGTCTGGAAGCGGCGGCGGGTCTGATGCCGCACCCGGAAATCGGT GCGCAGTCTCTGGAAGTTCCGCTGCGTCGTTCTAAACGTTTCAACTCTCACTTCCCGATC TGCTCTTACTGCTGCAACTGCTGCCGTAACAAAGGTTGCGGTCTGTGCTGCCGTACCTGA false false _768_ 0 16127 9 Not in stock false Optimized for E. coli K12 false Richard Decker BBa_M1721 1 BBa_M1721 Optimized Hepcidin + 6x spider silk + 10x His Tag 2013-04-03T11:00:00Z 2015-05-08T01:13:58Z Optimized sequence based on AA sequence available on NCBI for hepcidin from crocodile blood. This part was designed for a class and does not really exist. It is comprised of the part BBa_M1716, which is optimized hepcidin, 6 repeats of the part BBa_K844004, and part BBa_K844000, which is a 10x His tag. false false _768_ 0 16127 9 Not in stock false Optimzed for expression in E. coli. false Richard Decker component2226903 1 BBa_K844000 component2226899 1 BBa_M1720 annotation2226899 1 BBa_M1720 range2226899 1 1 1569 annotation2226903 1 BBa_K844000 range2226903 1 1578 1613 BBa_K844000 1 BBa_K844000 10x-Histidine (10x-His) Tag with double stop codon (TAATAA) 2012-10-01T11:00:00Z 2015-05-08T01:13:33Z Constructed through oligonucleotide annealing Released HQ 2013 10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part. false false _1104_ 0 9404 9 In stock true none false Kathleen Miller annotation2206607 1 10x-Histidine Tag range2206607 1 1 30 annotation2206609 1 Stop range2206609 1 34 36 annotation2206608 1 Stop range2206608 1 31 33 BBa_K844004 1 BBa_K844004 Spider Silk 1x Subunit "B" (Balanced tRNA codon optimized) 2012-10-01T11:00:00Z 2015-05-08T01:13:33Z Artificially designed based on BBa_K844002 and DNA was synthesized Spider silk subunit optimized to use a more balanced, but still greatly reduced, number of tRNA codons; contains two elasticity domains and one strength domain. false false _1104_ 0 13876 9 In stock false none false Andrea Halling annotation2212332 1 Strength Domain range2212332 1 186 204 annotation2212321 1 Beta-spiral range2212321 1 10 24 annotation2212323 1 Beta-spiral range2212323 1 46 60 annotation2212327 1 Beta-spiral range2212327 1 95 110 annotation2212325 1 Elasticity Domain range2212325 1 85 168 annotation2212326 1 Beta-helix range2212326 1 85 94 annotation2212322 1 Beta-spiral range2212322 1 31 45 annotation2212328 1 Beta-spiral range2212328 1 114 129 annotation2212329 1 Beta-spiral range2212329 1 130 144 annotation2212320 1 Beta-helix range2212320 1 1 9 annotation2212331 1 Linker Domain range2212331 1 169 185 annotation2212319 1 Elasticity Domain range2212319 1 1 84 annotation2212324 1 Beta-spiral range2212324 1 69 84 annotation2212330 1 Beta-spiral range2212330 1 153 168 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_M1721_sequence 1 atgaaagttctggcggcgtgcgttttcctgctgctgctggttctgctgcacggttctccggcggcgtgctctgcgctgcgtgcgcaggcgaacatcggtctgatgccgcgtccggaaaccggtgcgcagtctcacggtctggaagcggcggcgggtctgatgccgcacccggaaatcggtgcgcagtctctggaagttccgctgcgtcgttctaaacgtttcaactctcacttcccgatctgctcttactgctgcaactgctgccgtaacaaaggttgcggtctgtgctgccgtacctactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagcatcatcaccatcaccaccatcatcaccattaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K844004_sequence 1 ggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagca BBa_M1716_sequence 1 atgaaagttctggcggcgtgcgttttcctgctgctgctggttctgctgcacggttctccggcggcgtgctctgcgctgcgtgcgcaggcgaacatcggtctgatgccgcgtccggaaaccggtgcgcagtctcacggtctggaagcggcggcgggtctgatgccgcacccggaaatcggtgcgcagtctctggaagttccgctgcgtcgttctaaacgtttcaactctcacttcccgatctgctcttactgctgcaactgctgccgtaacaaaggttgcggtctgtgctgccgtacc BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_K844000_sequence 1 catcatcaccatcaccaccatcatcaccattaataa BBa_M1718_sequence 1 atgaaagttctggcggcgtgcgttttcctgctgctgctggttctgctgcacggttctccggcggcgtgctctgcgctgcgtgcgcaggcgaacatcggtctgatgccgcgtccggaaaccggtgcgcagtctcacggtctggaagcggcggcgggtctgatgccgcacccggaaatcggtgcgcagtctctggaagttccgctgcgtcgttctaaacgtttcaactctcacttcccgatctgctcttactgctgcaactgctgccgtaacaaaggttgcggtctgtgctgccgtacctactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagca BBa_M1727_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgaaagttctggcggcgtgcgttttcctgctgctgctggttctgctgcacggttctccggcggcgtgctctgcgctgcgtgcgcaggcgaacatcggtctgatgccgcgtccggaaaccggtgcgcagtctcacggtctggaagcggcggcgggtctgatgccgcacccggaaatcggtgcgcagtctctggaagttccgctgcgtcgttctaaacgtttcaactctcacttcccgatctgctcttactgctgcaactgctgccgtaacaaaggttgcggtctgtgctgccgtacctactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagcatcatcaccatcaccaccatcatcaccattaataa BBa_M1720_sequence 1 atgaaagttctggcggcgtgcgttttcctgctgctgctggttctgctgcacggttctccggcggcgtgctctgcgctgcgtgcgcaggcgaacatcggtctgatgccgcgtccggaaaccggtgcgcagtctcacggtctggaagcggcggcgggtctgatgccgcacccggaaatcggtgcgcagtctctggaagttccgctgcgtcgttctaaacgtttcaactctcacttcccgatctgctcttactgctgcaactgctgccgtaacaaaggttgcggtctgtgctgccgtacctactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z