##gff-version 3 ##sequence-region BBa_M1727 1 2849 BBa_M1727 . engineered_region 1237 2849 . + 0 ID=BBa_M1721;Name=BBa_M1721 BBa_M1727 . engineered_region 1237 2805 . + 0 ID=BBa_M1720;Name=BBa_M1720;Parent=BBa_M1721 BBa_M1727 . CDS 1754 1957 . + 0 ID=BBa_K844004;Name=BBa_K844004;Parent=BBa_M1720 BBa_M1727 . sequence_feature 1763 1777 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1799 1813 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1939 1957 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1848 1863 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1838 1921 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1822 1837 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1838 1847 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1883 1897 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1867 1882 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1922 1938 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1754 1762 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1754 1837 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1906 1921 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1784 1798 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . CDS 1966 2169 . + 0 ID=BBa_K844004;Name=BBa_K844004;Parent=BBa_M1720 BBa_M1727 . sequence_feature 1975 1989 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2011 2025 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2151 2169 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2060 2075 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2050 2133 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2034 2049 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2050 2059 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2095 2109 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2079 2094 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2134 2150 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1966 1974 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1966 2049 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2118 2133 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1996 2010 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . CDS 2602 2805 . + 0 ID=BBa_K844004;Name=BBa_K844004;Parent=BBa_M1720 BBa_M1727 . sequence_feature 2611 2625 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2647 2661 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2787 2805 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2696 2711 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2686 2769 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2670 2685 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2686 2695 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2731 2745 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2715 2730 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2770 2786 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2602 2610 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2602 2685 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2754 2769 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2632 2646 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . CDS 2178 2381 . + 0 ID=BBa_K844004;Name=BBa_K844004;Parent=BBa_M1720 BBa_M1727 . sequence_feature 2187 2201 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2223 2237 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2363 2381 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2272 2287 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2262 2345 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2246 2261 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2262 2271 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2307 2321 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2291 2306 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2346 2362 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2178 2186 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2178 2261 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2330 2345 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2208 2222 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . CDS 2390 2593 . + 0 ID=BBa_K844004;Name=BBa_K844004;Parent=BBa_M1720 BBa_M1727 . sequence_feature 2399 2413 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2435 2449 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2575 2593 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2484 2499 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2474 2557 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2458 2473 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2474 2483 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2519 2533 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2503 2518 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2558 2574 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2390 2398 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2390 2473 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2542 2557 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 2420 2434 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . engineered_region 1237 1745 . + 0 ID=BBa_M1718;Name=BBa_M1718;Parent=BBa_M1720 BBa_M1727 . CDS 1237 1533 . + 0 ID=BBa_M1716;Name=BBa_M1716;Parent=BBa_M1718 BBa_M1727 . CDS 1542 1745 . + 0 ID=BBa_K844004;Name=BBa_K844004;Parent=BBa_M1718 BBa_M1727 . sequence_feature 1551 1565 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1587 1601 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1727 1745 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1636 1651 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1626 1709 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1610 1625 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1626 1635 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1671 1685 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1655 1670 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1710 1726 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1542 1550 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1542 1625 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1694 1709 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . sequence_feature 1572 1586 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_M1727 . tag 2814 2849 . + 0 ID=BBa_K844000;Name=BBa_K844000;Parent=BBa_M1721 BBa_M1727 . stop_codon 2844 2846 . + 0 ID=annotation2206608;Name=Stop;Parent=BBa_K844000 BBa_M1727 . stop_codon 2847 2849 . + 0 ID=annotation2206609;Name=Stop;Parent=BBa_K844000 BBa_M1727 . sequence_feature 2814 2843 . + 0 ID=annotation2206607;Name=10x-Histidine Tag;Parent=BBa_K844000 BBa_M1727 . ribosome_entry_site 1219 1230 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_M1727 . conserved_region 1223 1226 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_M1727 . promoter 1 1210 . + 0 ID=BBa_I0500;Name=BBa_I0500 BBa_M1727 . sequence_feature 1 1079 . + 0 ID=annotation1498843;Name=AraC;Parent=BBa_I0500 BBa_M1727 . promoter 1043 1114 . + 0 ID=annotation1498842;Name=PC;Parent=BBa_I0500 BBa_M1727 . promoter 1080 1210 . + 0 ID=annotation1498841;Name=Pbad;Parent=BBa_I0500 >BBa_M1727 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgaaagttctggcggcgtgcgttttcc tgctgctgctggttctgctgcacggttctccggcggcgtgctctgcgctgcgtgcgcaggcgaacatcggtctgatgcc gcgtccggaaaccggtgcgcagtctcacggtctggaagcggcggcgggtctgatgccgcacccggaaatcggtgcgcag tctctggaagttccgctgcgtcgttctaaacgtttcaactctcacttcccgatctgctcttactgctgcaactgctgcc gtaacaaaggttgcggtctgtgctgccgtacctactagagggtggatatggtcctggagcaggtcaacaaggaccaggt agtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggac caggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagc agcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtc aacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctgg aggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatg gtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtgg atatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaa ggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggacc aggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaa ggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcag cagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctgga ggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggtt ctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtgga tatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaag gtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtgg acaaggtccttatggaccaagtgcagcagcagcagcagcatactagagcatcatcaccatcaccaccatcatcaccatt aataa