BBa_M1727
type: ComponentDefinition
persistent identity: BBa M1727
display id: BBa_M1727
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter + RBS + Hepcidin + Silk + His-Tag
created: 2013-04-12T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: From various sources.
owned by: james
owned by: myers
mutable description: This part was designed for a class and is not available. It contains a pBAD promoter, a standard RBS, the coding region for Hepcidin, a 6x repeat of spider silk, and a 10x histidine tag, followed by a double stop codon.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16127
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Hepcidin has been optimized for expression in E. coli
star: false
creator: Richard Decker
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M1721
component: BBa_B0034
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_M1721
sequence: https://synbiohub.org/public/igem/BBa_M1727_sequence/1

BBa_M1721
type: Component
persistent identity: component2231133
display id: component2231133
version: 1
top level: BBa_M1727
definition: BBa_M1721
access: public

BBa_B0034
type: Component
persistent identity: component2231035
display id: component2231035
version: 1
top level: BBa_M1727
definition: BBa_B0034
access: public

BBa_I0500
type: Component
persistent identity: component2231033
display id: component2231033
version: 1
top level: BBa_M1727
definition: pBad/araC
access: public

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2231033
display id: annotation2231033
version: 1
top level: BBa_M1727
location: https://synbiohub.org/public/igem/BBa_M1727/annotation2231033/range2231033/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_M1727/annotation2231033/range2231033/1
type: Range
persistent identity: range2231033
display id: range2231033
version: 1
top level: BBa_M1727
direction: direction/reverse
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2231035
display id: annotation2231035
version: 1
top level: BBa_M1727
location: https://synbiohub.org/public/igem/BBa_M1727/annotation2231035/range2231035/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M1727/annotation2231035/range2231035/1
type: Range
persistent identity: range2231035
display id: range2231035
version: 1
top level: BBa_M1727
direction: direction/reverse
start: 1219
end: 1230
orientation: inline

BBa_M1721
type: SequenceAnnotation
persistent identity: annotation2231133
display id: annotation2231133
version: 1
top level: BBa_M1727
location: https://synbiohub.org/public/igem/BBa_M1727/annotation2231133/range2231133/1
component: BBa_M1721

https://synbiohub.org/public/igem/BBa_M1727/annotation2231133/range2231133/1
type: Range
persistent identity: range2231133
display id: range2231133
version: 1
top level: BBa_M1727
direction: direction/reverse
start: 1237
end: 2849
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible pBad/araC promoter
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sri Kosuri
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: AraC
sequence annotation: PC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
top level: pBad/araC
direction: direction/forward
start: 1
end: 1079
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
top level: pBad/araC
direction: direction/forward
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
top level: pBad/araC
direction: direction/forward
start: 1080
end: 1210
orientation: inline

BBa_M1721
type: ComponentDefinition
persistent identity: BBa M1721
display id: BBa_M1721
version: 1
was generated by: iGEM to SBOL conversion
description: Optimized Hepcidin + 6x spider silk + 10x His Tag
created: 2013-04-03T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: Optimized sequence based on AA sequence available on NCBI for hepcidin from crocodile blood.
owned by: james
owned by: myers
mutable description: This part was designed for a class and does not really exist. It is comprised of the part BBa_M1716, which is optimized hepcidin, 6 repeats of the part BBa_K844004, and part BBa_K844000, which is a 10x His tag.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16127
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Optimzed for expression in E. coli.
star: false
creator: Richard Decker
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K844000
component: BBa_M1720
sequence annotation: BBa_M1720
sequence annotation: BBa_K844000
sequence: https://synbiohub.org/public/igem/BBa_M1721_sequence/1

BBa_K844000
type: Component
persistent identity: component2226903
display id: component2226903
version: 1
top level: BBa_M1721
definition: BBa_K844000
access: public

BBa_M1720
type: Component
persistent identity: component2226899
display id: component2226899
version: 1
top level: BBa_M1721
definition: BBa_M1720
access: public

BBa_M1720
type: SequenceAnnotation
persistent identity: annotation2226899
display id: annotation2226899
version: 1
top level: BBa_M1721
location: https://synbiohub.org/public/igem/BBa_M1721/annotation2226899/range2226899/1
component: BBa_M1720

https://synbiohub.org/public/igem/BBa_M1721/annotation2226899/range2226899/1
type: Range
persistent identity: range2226899
display id: range2226899
version: 1
top level: BBa_M1721
direction: direction/reverse
start: 1
end: 1569
orientation: inline

BBa_K844000
type: SequenceAnnotation
persistent identity: annotation2226903
display id: annotation2226903
version: 1
top level: BBa_M1721
location: https://synbiohub.org/public/igem/BBa_M1721/annotation2226903/range2226903/1
component: BBa_K844000

https://synbiohub.org/public/igem/BBa_M1721/annotation2226903/range2226903/1
type: Range
persistent identity: range2226903
display id: range2226903
version: 1
top level: BBa_M1721
direction: direction/reverse
start: 1578
end: 1613
orientation: inline

BBa_K844000
type: ComponentDefinition
persistent identity: BBa K844000
display id: BBa_K844000
version: 1
was generated by: iGEM to SBOL conversion
description: 10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:33Z
mutable provenance: Constructed through oligonucleotide annealing
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: 10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1104_
m user id: 0
owner id: 9404
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: none
star: false
creator: Kathleen Miller
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: 10x-Histidine Tag
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K844000_sequence/1

10x-Histidine Tag
type: SequenceAnnotation
persistent identity: annotation2206607
display id: annotation2206607
version: 1
top level: BBa_K844000
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206607/range2206607/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K844000/annotation2206607/range2206607/1
type: Range
persistent identity: range2206607
display id: range2206607
version: 1
top level: BBa_K844000
direction: direction/forward
start: 1
end: 30
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2206608
display id: annotation2206608
version: 1
top level: BBa_K844000
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206608/range2206608/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K844000/annotation2206608/range2206608/1
type: Range
persistent identity: range2206608
display id: range2206608
version: 1
top level: BBa_K844000
direction: direction/forward
start: 31
end: 33
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2206609
display id: annotation2206609
version: 1
top level: BBa_K844000
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206609/range2206609/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K844000/annotation2206609/range2206609/1
type: Range
persistent identity: range2206609
display id: range2206609
version: 1
top level: BBa_K844000
direction: direction/forward
start: 34
end: 36
orientation: inline

BBa_M1720
type: ComponentDefinition
persistent identity: BBa M1720
display id: BBa_M1720
version: 1
was generated by: iGEM to SBOL conversion
description: Optimized Hepcidin + 6x spider silk
created: 2013-04-03T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: The part comes from the AA sequence for hepcidin, but has been optimized for E. coli.
owned by: james
owned by: myers
mutable description: This part was designed for a class and does not really exist. It is comprised of the part BBa_M1718, which is a combination of Optimized hepcidin and spider silk, and 5 repeats of the part BBa_K844004.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16127
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Optimized for E. coli using NCBI OPTIMIZER.
star: false
creator: Richard Decker
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K844004
component: BBa_M1718
sequence annotation: BBa_M1718
sequence annotation: BBa_K844004
sequence: https://synbiohub.org/public/igem/BBa_M1720_sequence/1

BBa_K844004
type: Component
persistent identity: component2225515
display id: component2225515
version: 1
top level: BBa_M1720
definition: BBa_K844004
access: public

BBa_M1718
type: Component
persistent identity: component2225500
display id: component2225500
version: 1
top level: BBa_M1720
definition: BBa_M1718
access: public

BBa_K844004
type: Component
persistent identity: component2225575
display id: component2225575
version: 1
top level: BBa_M1720
definition: BBa_K844004
access: public

BBa_K844004
type: Component
persistent identity: component2225560
display id: component2225560
version: 1
top level: BBa_M1720
definition: BBa_K844004
access: public

BBa_K844004
type: Component
persistent identity: component2225545
display id: component2225545
version: 1
top level: BBa_M1720
definition: BBa_K844004
access: public

BBa_K844004
type: Component
persistent identity: component2225530
display id: component2225530
version: 1
top level: BBa_M1720
definition: BBa_K844004
access: public

BBa_M1718
type: SequenceAnnotation
persistent identity: annotation2225500
display id: annotation2225500
version: 1
top level: BBa_M1720
location: https://synbiohub.org/public/igem/BBa_M1720/annotation2225500/range2225500/1
component: BBa_M1718

https://synbiohub.org/public/igem/BBa_M1720/annotation2225500/range2225500/1
type: Range
persistent identity: range2225500
display id: range2225500
version: 1
top level: BBa_M1720
direction: direction/reverse
start: 1
end: 509
orientation: inline

BBa_K844004
type: SequenceAnnotation
persistent identity: annotation2225515
display id: annotation2225515
version: 1
top level: BBa_M1720
location: https://synbiohub.org/public/igem/BBa_M1720/annotation2225515/range2225515/1
component: BBa_K844004

https://synbiohub.org/public/igem/BBa_M1720/annotation2225515/range2225515/1
type: Range
persistent identity: range2225515
display id: range2225515
version: 1
top level: BBa_M1720
direction: direction/reverse
start: 518
end: 721
orientation: inline

BBa_K844004
type: SequenceAnnotation
persistent identity: annotation2225530
display id: annotation2225530
version: 1
top level: BBa_M1720
location: https://synbiohub.org/public/igem/BBa_M1720/annotation2225530/range2225530/1
component: BBa_K844004

https://synbiohub.org/public/igem/BBa_M1720/annotation2225530/range2225530/1
type: Range
persistent identity: range2225530
display id: range2225530
version: 1
top level: BBa_M1720
direction: direction/reverse
start: 730
end: 933
orientation: inline

BBa_K844004
type: SequenceAnnotation
persistent identity: annotation2225545
display id: annotation2225545
version: 1
top level: BBa_M1720
location: https://synbiohub.org/public/igem/BBa_M1720/annotation2225545/range2225545/1
component: BBa_K844004

https://synbiohub.org/public/igem/BBa_M1720/annotation2225545/range2225545/1
type: Range
persistent identity: range2225545
display id: range2225545
version: 1
top level: BBa_M1720
direction: direction/reverse
start: 942
end: 1145
orientation: inline

BBa_K844004
type: SequenceAnnotation
persistent identity: annotation2225560
display id: annotation2225560
version: 1
top level: BBa_M1720
location: https://synbiohub.org/public/igem/BBa_M1720/annotation2225560/range2225560/1
component: BBa_K844004

https://synbiohub.org/public/igem/BBa_M1720/annotation2225560/range2225560/1
type: Range
persistent identity: range2225560
display id: range2225560
version: 1
top level: BBa_M1720
direction: direction/reverse
start: 1154
end: 1357
orientation: inline

BBa_K844004
type: SequenceAnnotation
persistent identity: annotation2225575
display id: annotation2225575
version: 1
top level: BBa_M1720
location: https://synbiohub.org/public/igem/BBa_M1720/annotation2225575/range2225575/1
component: BBa_K844004

https://synbiohub.org/public/igem/BBa_M1720/annotation2225575/range2225575/1
type: Range
persistent identity: range2225575
display id: range2225575
version: 1
top level: BBa_M1720
direction: direction/reverse
start: 1366
end: 1569
orientation: inline

BBa_K844004
type: ComponentDefinition
persistent identity: BBa K844004
display id: BBa_K844004
version: 1
was generated by: iGEM to SBOL conversion
description: Spider Silk 1x Subunit "B" (Balanced tRNA codon optimized)
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:33Z
mutable provenance: Artificially designed based on BBa_K844002 and DNA was synthesized
owned by: james
owned by: myers
mutable description: Spider silk subunit optimized to use a more balanced, but still greatly reduced, number of tRNA codons; contains two elasticity domains and one strength domain.
discontinued: false
dominant: false
group u list: _1104_
m user id: 0
owner id: 13876
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: none
star: false
creator: Andrea Halling
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Elasticity Domain
sequence annotation: Beta-helix
sequence annotation: Beta-spiral
sequence annotation: Linker Domain
sequence annotation: Strength Domain
sequence: https://synbiohub.org/public/igem/BBa_K844004_sequence/1

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212319
display id: annotation2212319
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212319/range2212319/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K844004/annotation2212319/range2212319/1
type: Range
persistent identity: range2212319
display id: range2212319
version: 1
top level: BBa_K844004
direction: direction/forward
start: 1
end: 84
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212320
display id: annotation2212320
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212320/range2212320/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K844004/annotation2212320/range2212320/1
type: Range
persistent identity: range2212320
display id: range2212320
version: 1
top level: BBa_K844004
direction: direction/forward
start: 1
end: 9
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212321
display id: annotation2212321
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212321/range2212321/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K844004/annotation2212321/range2212321/1
type: Range
persistent identity: range2212321
display id: range2212321
version: 1
top level: BBa_K844004
direction: direction/forward
start: 10
end: 24
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212322
display id: annotation2212322
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212322/range2212322/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K844004/annotation2212322/range2212322/1
type: Range
persistent identity: range2212322
display id: range2212322
version: 1
top level: BBa_K844004
direction: direction/forward
start: 31
end: 45
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212323
display id: annotation2212323
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212323/range2212323/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K844004/annotation2212323/range2212323/1
type: Range
persistent identity: range2212323
display id: range2212323
version: 1
top level: BBa_K844004
direction: direction/forward
start: 46
end: 60
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212324
display id: annotation2212324
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212324/range2212324/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K844004/annotation2212324/range2212324/1
type: Range
persistent identity: range2212324
display id: range2212324
version: 1
top level: BBa_K844004
direction: direction/forward
start: 69
end: 84
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212325
display id: annotation2212325
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212325/range2212325/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K844004/annotation2212325/range2212325/1
type: Range
persistent identity: range2212325
display id: range2212325
version: 1
top level: BBa_K844004
direction: direction/forward
start: 85
end: 168
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212326
display id: annotation2212326
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212326/range2212326/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K844004/annotation2212326/range2212326/1
type: Range
persistent identity: range2212326
display id: range2212326
version: 1
top level: BBa_K844004
direction: direction/forward
start: 85
end: 94
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212327
display id: annotation2212327
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212327/range2212327/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K844004/annotation2212327/range2212327/1
type: Range
persistent identity: range2212327
display id: range2212327
version: 1
top level: BBa_K844004
direction: direction/forward
start: 95
end: 110
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212328
display id: annotation2212328
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212328/range2212328/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K844004/annotation2212328/range2212328/1
type: Range
persistent identity: range2212328
display id: range2212328
version: 1
top level: BBa_K844004
direction: direction/forward
start: 114
end: 129
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212329
display id: annotation2212329
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212329/range2212329/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K844004/annotation2212329/range2212329/1
type: Range
persistent identity: range2212329
display id: range2212329
version: 1
top level: BBa_K844004
direction: direction/forward
start: 130
end: 144
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212330
display id: annotation2212330
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212330/range2212330/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K844004/annotation2212330/range2212330/1
type: Range
persistent identity: range2212330
display id: range2212330
version: 1
top level: BBa_K844004
direction: direction/forward
start: 153
end: 168
orientation: inline

Linker Domain
type: SequenceAnnotation
persistent identity: annotation2212331
display id: annotation2212331
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212331/range2212331/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K844004/annotation2212331/range2212331/1
type: Range
persistent identity: range2212331
display id: range2212331
version: 1
top level: BBa_K844004
direction: direction/forward
start: 169
end: 185
orientation: inline

Strength Domain
type: SequenceAnnotation
persistent identity: annotation2212332
display id: annotation2212332
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212332/range2212332/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K844004/annotation2212332/range2212332/1
type: Range
persistent identity: range2212332
display id: range2212332
version: 1
top level: BBa_K844004
direction: direction/forward
start: 186
end: 204
orientation: inline

BBa_M1718
type: ComponentDefinition
persistent identity: BBa M1718
display id: BBa_M1718
version: 1
was generated by: iGEM to SBOL conversion
description: Optimized Hepcidin + 1x spider silk
created: 2013-03-31T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: Both protein coding regions are based on original AA sequences, but have been optimized for expression in E. coli.
owned by: james
owned by: myers
mutable description: This part is for a class project and does not really exist. The part combines the part BBa_M1716 that codes for the E. coli optimized hepcidin protein with part BBa_K844004, which codes for a single coding region of spider silk protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 16127
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No considerations of note.
star: false
creator: Richard Decker
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K844004
component: BBa_M1716
sequence annotation: BBa_M1716
sequence annotation: BBa_K844004
sequence: https://synbiohub.org/public/igem/BBa_M1718_sequence/1

BBa_K844004
type: Component
persistent identity: component2219134
display id: component2219134
version: 1
top level: BBa_M1718
definition: BBa_K844004
access: public

BBa_M1716
type: Component
persistent identity: component2219119
display id: component2219119
version: 1
top level: BBa_M1718
definition: BBa_M1716
access: public

BBa_M1716
type: SequenceAnnotation
persistent identity: annotation2219119
display id: annotation2219119
version: 1
top level: BBa_M1718
location: https://synbiohub.org/public/igem/BBa_M1718/annotation2219119/range2219119/1
component: BBa_M1716

https://synbiohub.org/public/igem/BBa_M1718/annotation2219119/range2219119/1
type: Range
persistent identity: range2219119
display id: range2219119
version: 1
top level: BBa_M1718
direction: direction/reverse
start: 1
end: 297
orientation: inline

BBa_K844004
type: SequenceAnnotation
persistent identity: annotation2219134
display id: annotation2219134
version: 1
top level: BBa_M1718
location: https://synbiohub.org/public/igem/BBa_M1718/annotation2219134/range2219134/1
component: BBa_K844004

https://synbiohub.org/public/igem/BBa_M1718/annotation2219134/range2219134/1
type: Range
persistent identity: range2219134
display id: range2219134
version: 1
top level: BBa_M1718
direction: direction/reverse
start: 306
end: 509
orientation: inline

BBa_M1716
type: ComponentDefinition
persistent identity: BBa M1716
display id: BBa_M1716
version: 1
was generated by: iGEM to SBOL conversion
description: Hepcidin Optimized for E. coli
created: 2013-03-31T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: NCBI OPTIMIZER using AA sequence from GenBank number ADA68357.
owned by: james
owned by: myers
mutable description: ATGAAAGTTCTGGCGGCGTGCGTTTTCCTGCTGCTGCTGGTTCTGCTGCACGGTTCTCCG GCGGCGTGCTCTGCGCTGCGTGCGCAGGCGAACATCGGTCTGATGCCGCGTCCGGAAACC GGTGCGCAGTCTCACGGTCTGGAAGCGGCGGCGGGTCTGATGCCGCACCCGGAAATCGGT GCGCAGTCTCTGGAAGTTCCGCTGCGTCGTTCTAAACGTTTCAACTCTCACTTCCCGATC TGCTCTTACTGCTGCAACTGCTGCCGTAACAAAGGTTGCGGTCTGTGCTGCCGTACCTGA
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 16127
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Optimized for E. coli K12
star: false
creator: Richard Decker
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_M1716_sequence/1

https://synbiohub.org/public/igem/BBa_M1727_sequence/1
type: Sequence
persistent identity: BBa M1727 sequence
display id: BBa_M1727_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgaaagttctggcggcgtgcgttttcctgctgctgctggttctgctgcacggttctccggcggcgtgctctgcgctgcgtgcgcaggcgaacatcggtctgatgccgcgtccggaaaccggtgcgcagtctcacggtctggaagcggcggcgggtctgatgccgcacccggaaatcggtgcgcagtctctggaagttccgctgcgtcgttctaaacgtttcaactctcacttcccgatctgctcttactgctgcaactgctgccgtaacaaaggttgcggtctgtgctgccgtacctactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggacc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0500_sequence/1
type: Sequence
persistent identity: BBa I0500 sequence
display id: BBa_I0500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1721_sequence/1
type: Sequence
persistent identity: BBa M1721 sequence
display id: BBa_M1721_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaagttctggcggcgtgcgttttcctgctgctgctggttctgctgcacggttctccggcggcgtgctctgcgctgcgtgcgcaggcgaacatcggtctgatgccgcgtccggaaaccggtgcgcagtctcacggtctggaagcggcggcgggtctgatgccgcacccggaaatcggtgcgcagtctctggaagttccgctgcgtcgttctaaacgtttcaactctcacttcccgatctgctcttactgctgcaactgctgccgtaacaaaggttgcggtctgtgctgccgtacctactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagcatcatcaccatcaccaccatcatcaccattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K844000_sequence/1
type: Sequence
persistent identity: BBa K844000 sequence
display id: BBa_K844000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catcatcaccatcaccaccatcatcaccattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1720_sequence/1
type: Sequence
persistent identity: BBa M1720 sequence
display id: BBa_M1720_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaagttctggcggcgtgcgttttcctgctgctgctggttctgctgcacggttctccggcggcgtgctctgcgctgcgtgcgcaggcgaacatcggtctgatgccgcgtccggaaaccggtgcgcagtctcacggtctggaagcggcggcgggtctgatgccgcacccggaaatcggtgcgcagtctctggaagttccgctgcgtcgttctaaacgtttcaactctcacttcccgatctgctcttactgctgcaactgctgccgtaacaaaggttgcggtctgtgctgccgtacctactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K844004_sequence/1
type: Sequence
persistent identity: BBa K844004 sequence
display id: BBa_K844004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1718_sequence/1
type: Sequence
persistent identity: BBa M1718 sequence
display id: BBa_M1718_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaagttctggcggcgtgcgttttcctgctgctgctggttctgctgcacggttctccggcggcgtgctctgcgctgcgtgcgcaggcgaacatcggtctgatgccgcgtccggaaaccggtgcgcagtctcacggtctggaagcggcggcgggtctgatgccgcacccggaaatcggtgcgcagtctctggaagttccgctgcgtcgttctaaacgtttcaactctcacttcccgatctgctcttactgctgcaactgctgccgtaacaaaggttgcggtctgtgctgccgtacctactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1716_sequence/1
type: Sequence
persistent identity: BBa M1716 sequence
display id: BBa_M1716_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaagttctggcggcgtgcgttttcctgctgctgctggttctgctgcacggttctccggcggcgtgctctgcgctgcgtgcgcaggcgaacatcggtctgatgccgcgtccggaaaccggtgcgcagtctcacggtctggaagcggcggcgggtctgatgccgcacccggaaatcggtgcgcagtctctggaagttccgctgcgtcgttctaaacgtttcaactctcacttcccgatctgctcttactgctgcaactgctgccgtaacaaaggttgcggtctgtgctgccgtacc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z