LOCUS       BBa_M1729               2420 bp    DNA     linear       22-Nov-2024
DEFINITION  GFP reporter expression after induction with pBAD and hepcidin.
ACCESSION   BBa_M1729
VERSION     BBa_M1729.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1536
                     /label=BBa_M1728
     RBS             1545..1557
                     /label=RBS-3\Weak
     RBS             1551..1554
                     /label=RBS
     misc_feature    1545..1557
                     /label=BBa_B0032
     RBS             1545..1557
                     /label=BBa_B0032
     CDS             1564..2283
                     /label=GFP protein
     CDS             1564..2283
                     /label=BBa_E0040
     stem_loop       2303..2346
                     /label=stem_loop
     misc_feature    2292..2371
                     /label=BBa_B0010
     terminator      2292..2371
                     /label=BBa_B0010
     stem_loop       2387..2406
                     /label=T7 TE
     misc_feature    2413..2413
                     /label=stop
     polyA_site      2407..2420
                     /label=polya
     misc_feature    2380..2420
                     /label=BBa_B0012
     terminator      2380..2420
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga aagttctggc ggcgtgcgtt
     1261 ttcctgctgc tgctggttct gctgcacggt tctccggcgg cgtgctctgc gctgcgtgcg
     1321 caggcgaaca tcggtctgat gccgcgtccg gaaaccggtg cgcagtctca cggtctggaa
     1381 gcggcggcgg gtctgatgcc gcacccggaa atcggtgcgc agtctctgga agttccgctg
     1441 cgtcgttcta aacgtttcaa ctctcacttc ccgatctgct cttactgctg caactgctgc
     1501 cgtaacaaag gttgcggtct gtgctgccgt acctgatact agagtcacac aggaaagtac
     1561 tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
     1621 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
     1681 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
     1741 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
     1801 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
     1861 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
     1921 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
     1981 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
     2041 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
     2101 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
     2161 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
     2221 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa
     2281 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2341 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     2401 tgggcctttc tgcgtttata
//