GFP reporter expression after induction with pBAD and hepcidin.
Hepcidin comes from crocodile. It has been codon optimized.
This part is created in theory for a synthetic bio class. It will test the induction of GFP with hepcidin using the pBAD promoter to see the optimial concentration of arabinose needed.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
RBS.3 (medium) -- derivative of BBa_0030
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
Transcriptional terminator consisting of a 64 bp stem-loop.
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
GFP (mut3b) [note that this part does not have a barcode]
Hepcidin Optimized for E. coli with stop codon
atgaaagttctggcggcgtgcgttttcctgctgctgctggttctgctgcacggttctccggcggcgtgctctgcgctgcgtgcgcaggcgaacatcggtc tgatgccgcgtccggaaaccggtgcgcagtctcacggtctggaagcggcggcgggtctgatgccgcacccggaaatcggtgcgcagtctctggaagttcc gctgcgtcgttctaaacgtttcaactctcacttcccgatctgctcttactgctgcaactgctgccgtaacaaaggttgcggtctgtgctgccgtacctga
Conversion of the iGEM parts registry to SBOL2.1