LOCUS       BBa_M1730               1714 bp    DNA     linear       26-Nov-2024
DEFINITION  pBad with optimized hepcidin along with a histag
ACCESSION   BBa_M1730
VERSION     BBa_M1730.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1533
                     /label=BBa_M1716
     misc_feature    1542..1571
                     /label=10x-Histidine Tag
     misc_feature    1572..1574
                     /label=Stop
     misc_feature    1575..1577
                     /label=Stop
     misc_feature    1542..1577
                     /label=BBa_K844000
     stem_loop       1597..1640
                     /label=stem_loop
     misc_feature    1586..1665
                     /label=BBa_B0010
     terminator      1586..1665
                     /label=BBa_B0010
     stem_loop       1681..1700
                     /label=T7 TE
     misc_feature    1707..1707
                     /label=stop
     polyA_site      1701..1714
                     /label=polya
     misc_feature    1674..1714
                     /label=BBa_B0012
     terminator      1674..1714
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga aagttctggc ggcgtgcgtt
     1261 ttcctgctgc tgctggttct gctgcacggt tctccggcgg cgtgctctgc gctgcgtgcg
     1321 caggcgaaca tcggtctgat gccgcgtccg gaaaccggtg cgcagtctca cggtctggaa
     1381 gcggcggcgg gtctgatgcc gcacccggaa atcggtgcgc agtctctgga agttccgctg
     1441 cgtcgttcta aacgtttcaa ctctcacttc ccgatctgct cttactgctg caactgctgc
     1501 cgtaacaaag gttgcggtct gtgctgccgt acctactaga gcatcatcac catcaccacc
     1561 atcatcacca ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1621 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1681 ggctcacctt cgggtgggcc tttctgcgtt tata
//