##gff-version 3
##sequence-region BBa_M1838 1 1892
BBa_M1838	.	ribosome_entry_site	751	762	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_M1838	.	conserved_region	755	758	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_M1838	.	terminator	1764	1843	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_M1838	.	engineered_region	1764	1843	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1838	.	stem_loop	1775	1818	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1838	.	CDS	769	1035	.	+	0	ID=BBa_M1986;Name=BBa_M1986
BBa_M1838	.	start_codon	769	771	.	+	0	ID=annotation2171187;Name=Start codon;Parent=BBa_M1986
BBa_M1838	.	CDS	772	1032	.	+	0	ID=annotation2171189;Name=Open reading frame;Parent=BBa_M1986
BBa_M1838	.	stop_codon	1033	1035	.	+	0	ID=annotation2171188;Name=Stop Codon;Parent=BBa_M1986
BBa_M1838	.	promoter	1	742	.	+	0	ID=BBa_K123002;Name=BBa_K123002
BBa_M1838	.	engineered_region	704	728	.	+	0	ID=annotation1992724;Name=barcode;Parent=BBa_K123002
BBa_M1838	.	engineered_region	1	37	.	+	0	ID=annotation1992720;Name=BBa_I14032;Parent=BBa_K123002
BBa_M1838	.	promoter	3	8	.	+	0	ID=annotation1992718;Name=-35;Parent=BBa_K123002
BBa_M1838	.	tag	664	697	.	+	0	ID=annotation1992722;Name=SsrA;Parent=BBa_K123002
BBa_M1838	.	promoter	26	31	.	+	0	ID=annotation1992719;Name=-10;Parent=BBa_K123002
BBa_M1838	.	engineered_region	44	703	.	+	0	ID=annotation1992723;Name=BBa_C0040;Parent=BBa_K123002
BBa_M1838	.	CDS	47	663	.	+	0	ID=annotation1992721;Name=tetR;Parent=BBa_K123002
BBa_M1838	.	non_covalent_binding_site	43	57	.	+	0	ID=annotation1992733;Name=ERE;Parent=BBa_K123002
BBa_M1838	.	operator	1	37	.	+	0	ID=annotation1992717;Name=P(Lac) IQ;Parent=BBa_K123002
BBa_M1838	.	sequence_feature	1042	1755	.	+	0	ID=BBa_M1871;Name=BBa_M1871
BBa_M1838	.	stop_codon	1541	1641	.	+	0	ID=annotation2171460;Name=stop;Parent=BBa_M1871
BBa_M1838	.	start_codon	1242	1441	.	+	0	ID=annotation2171459;Name=start;Parent=BBa_M1871
BBa_M1838	.	operator	1042	1241	.	+	0	ID=annotation2171458;Name=operator;Parent=BBa_M1871
BBa_M1838	.	terminator	1852	1892	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_M1838	.	engineered_region	1852	1892	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1838	.	stop_codon	1885	1885	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1838	.	stem_loop	1859	1878	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1838	.	polyA_site	1879	1892	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_M1838
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagta
aagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagct
aggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgtta
gataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagtttta
gatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactct
cgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcat
tttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagta
tgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaatt
gatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagct
taataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggccaacaccgtgtcggcca
agaagatgacccgcaagatcgccaagcgtacggcgatcaaccgctcgcgccgttcgcggatgcgtaccttcgtccgcaa
ggtcgaggaggcgattgcgtccggcgatcagggccaggccctcaccgccctccgcgccgccgagccggagatcatgcgc
gctgcccagaacggcatcgttcacaagaacaatgcctcgcggaaggtttcccgcctggccgcccgcgtgaaggccatcg
cggcctgatactagatgagtaaaggagaagaacttttcactggagtggtcccagttcttgttgaattagatggcgatgt
taatgggcaaaaattctctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaattttatttgc
actactgggaagctacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttctcaagatacc
cagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatattttacaa
agatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggt
attgattttaaagaagatggaaacattcttggacacaaaatggaatacaactataactcacataatgtatacatcatgg
gagacaaaccaaagaatggcatcaaagttaacttcaaaattagacacaacattaaagatggaagcgttcaattagcaga
ccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgcc
ctttccaaagatcccaacgaaaagagagatcacatgatccttcttgagtttgtaacagctgctaggattacacatggca
tggatgaactatacaaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata