RPS Tgene from Methilobacterium chloromeneticum
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
rpsT gene from Methylobacterium Chloromethanicum
Methylobacterium Chloromethanicum. The sequence comes from the site http://www.ncbi.nlm.nih.gov/nuccore/218528082?from=359&to=625
The rpsT gene binds directly to the 16s rRNA and induces post translational inhibition of arginine and ornithine decarboxylase
I copied and pasted the sequence from NCBI.
atgagtaaag gagaagaact tttcactgga gtggtcccag ttcttgttga attagatggc gatgttaatg ggcaaaaatt ctctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaattttat ttgcactact gggaagctac ctgttccatg gccaacactt gtcactactt tctcttatgg tgttcaatgc ttctcaagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatattttac aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa atggaataca actataactc acataatgta tacatcatgg gagacaaacc aaagaatggc atcaaagtta acttcaaaat tagacacaac attaaagatg gaagcgttca attagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttccaaagat cccaacgaaa agagagatca catgatcctt cttgagtttg taacagctgc taggattaca catggcatgg atgaactata caaa
The Estrogen Responsive Element was taken from the Human Estrogen Responsive Element
This composite part contains LacIQ (BBa_I14032) and Estrogen Receptor Element (ERE) and TetR (BBa_C0040). The Estrogen Responsive element is to be used in conjunction with ER (Estrogen Receptor). This design involves ER binding to the ERE and disrupting the constituative promoter LacIQ from producing TetR via physically getting in the way of the polymerase.
There is some data that suggests the ER is toxic to the cell. However in our experiments while we did see a decrease in growth rate we did not find ER to be fatal to the cell.
Conversion of the iGEM parts registry to SBOL2.1