BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K123002
1
BBa_K123002
LacIQ ERE TetR
2008-10-27T12:00:00Z
2015-05-08T01:09:44Z
The Estrogen Responsive Element was taken from the Human Estrogen Responsive Element
This composite part contains LacIQ (BBa_I14032) and Estrogen Receptor Element (ERE) and TetR (BBa_C0040). The Estrogen Responsive element is to be used in conjunction with ER (Estrogen Receptor). This design involves ER binding to the ERE and disrupting the constituative promoter LacIQ from producing TetR via physically getting in the way of the polymerase.
false
false
_241_
0
2742
9
It's complicated
true
There is some data that suggests the ER is toxic to the cell. However in our experiments while we did see a decrease in growth rate we did not find ER to be fatal to the cell.
true
Jason Gardiner
annotation1992722
1
SsrA
range1992722
1
664
697
annotation1992723
1
BBa_C0040
range1992723
1
44
703
annotation1992719
1
-10
range1992719
1
26
31
annotation1992718
1
-35
range1992718
1
3
8
annotation1992733
1
ERE
range1992733
1
43
57
annotation1992717
1
P(Lac) IQ
range1992717
1
1
37
annotation1992721
1
tetR
range1992721
1
47
663
annotation1992720
1
BBa_I14032
range1992720
1
1
37
annotation1992724
1
barcode
range1992724
1
704
728
BBa_M1986
1
BBa_M1986
rpsT gene from Methylobacterium Chloromethanicum
2012-03-26T11:00:00Z
2015-05-08T01:13:58Z
Methylobacterium Chloromethanicum. The sequence comes from the site http://www.ncbi.nlm.nih.gov/nuccore/218528082?from=359&to=625
The rpsT gene binds directly to the 16s rRNA and induces post translational inhibition of arginine and ornithine
decarboxylase
false
false
_768_
0
12223
9
Not in stock
false
I copied and pasted the sequence from NCBI.
false
David Harris
annotation2171189
1
Open reading frame
range2171189
1
4
264
annotation2171187
1
Start codon
range2171187
1
1
3
annotation2171188
1
Stop Codon
range2171188
1
265
267
BBa_M1839
1
BBa_M1839
gfgf
2012-03-27T11:00:00Z
2015-05-08T01:13:58Z
fgg
gfg
true
false
_768_
0
12257
9
Discontinued
false
gfg
false
sarah guzman
component2171671
1
BBa_K123002
component2171677
1
BBa_M1986
component2171673
1
BBa_B0034
component2171680
1
BBa_B0012
component2171678
1
BBa_B0010
annotation2171673
1
BBa_B0034
range2171673
1
751
762
annotation2171678
1
BBa_B0010
range2171678
1
1044
1123
annotation2171680
1
BBa_B0012
range2171680
1
1132
1172
annotation2171671
1
BBa_K123002
range2171671
1
1
742
annotation2171677
1
BBa_M1986
range2171677
1
769
1035
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K123002_sequence
1
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
BBa_B0034_sequence
1
aaagaggagaaa
BBa_M1986_sequence
1
atggccaacaccgtgtcggccaagaagatgacccgcaagatcgccaagcgtacggcgatcaaccgctcgcgccgttcgcggatgcgtaccttcgtccgcaaggtcgaggaggcgattgcgtccggcgatcagggccaggccctcaccgccctccgcgccgccgagccggagatcatgcgcgctgcccagaacggcatcgttcacaagaacaatgcctcgcggaaggtttcccgcctggccgcccgcgtgaaggccatcgcggcctga
BBa_M1839_sequence
1
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggccaacaccgtgtcggccaagaagatgacccgcaagatcgccaagcgtacggcgatcaaccgctcgcgccgttcgcggatgcgtaccttcgtccgcaaggtcgaggaggcgattgcgtccggcgatcagggccaggccctcaccgccctccgcgccgccgagccggagatcatgcgcgctgcccagaacggcatcgttcacaagaacaatgcctcgcggaaggtttcccgcctggccgcccgcgtgaaggccatcgcggcctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z