##gff-version 3 ##sequence-region BBa_M11107 1 3 ##sequence-region BBa_M1843 1 5712 BBa_M11107 . start_codon 1 3 . + 0 ID=annotation2067582;Name=start ATG BBa_M1843 . engineered_region 726 1678 . + 0 ID=BBa_M1887;Name=BBa_M1887 BBa_M1843 . engineered_region 746 1648 . + 0 ID=BBa_M1999;Name=BBa_M1999;Parent=BBa_M1887 BBa_M1843 . promoter 746 799 . + 0 ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_M1999 BBa_M1843 . promoter 765 770 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_M1843 . non_covalent_binding_site 771 789 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 BBa_M1843 . engineered_region 746 799 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_M1843 . promoter 788 793 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_M1843 . non_covalent_binding_site 746 764 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_M1843 . terminator 1520 1599 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_M1999 BBa_M1843 . engineered_region 1520 1599 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_M1843 . stem_loop 1531 1574 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_M1843 . sequence_feature 826 1511 . + 0 ID=BBa_M1998;Name=BBa_M1998;Parent=BBa_M1999 BBa_M1843 . sequence_feature 1205 1225 . + 0 ID=annotation2170793;Name=just for fun;Parent=BBa_M1998 BBa_M1843 . start_codon 826 828 . + 0 ID=annotation2170800;Name=start codon;Parent=BBa_M1998 BBa_M1843 . CDS 826 1511 . + 0 ID=annotation2170787;Name=cds;Parent=BBa_M1998 BBa_M1843 . ribosome_entry_site 808 819 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_M1999 BBa_M1843 . conserved_region 812 815 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_M1843 . terminator 1608 1648 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_M1999 BBa_M1843 . engineered_region 1608 1648 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_M1843 . stop_codon 1641 1641 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_M1843 . stem_loop 1615 1634 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_M1843 . polyA_site 1635 1648 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_M1843 . sequence_feature 726 737 . + 0 ID=BBa_M1877;Name=BBa_M1877;Parent=BBa_M1887 BBa_M1843 . stop_codon 737 737 . + 0 ID=annotation2171248;Name=insulin stop ;Parent=BBa_M1877 BBa_M1843 . start_codon 726 726 . + 0 ID=annotation2171242;Name=insulin start ;Parent=BBa_M1877 BBa_M1843 . CDS 726 737 . + 0 ID=annotation2171241;Name=coding sequence ;Parent=BBa_M1877 BBa_M1843 . CDS 726 737 . + 0 ID=annotation2171243;Name=insulin ;Parent=BBa_M1877 BBa_M1843 . sequence_feature 1657 1678 . + 0 ID=BBa_M1000;Name=BBa_M1000;Parent=BBa_M1887 BBa_M1843 . sequence_feature 1660 1678 . + 0 ID=annotation1825569;Name=Mosaic End (forward);Parent=BBa_M1000 BBa_M1843 . sequence_feature 1657 1662 . + 0 ID=annotation1825568;Name=PvuII cut site;Parent=BBa_M1000 BBa_M1843 . engineered_region 1687 1698 . + 0 ID=BBa_M1888;Name=BBa_M1888 BBa_M1843 . stop_codon 1698 1698 . + 0 ID=annotation2171254;Name=insulin stop ;Parent=BBa_M1888 BBa_M1843 . CDS 1687 1698 . + 0 ID=annotation2171251;Name=coding sequence ;Parent=BBa_M1888 BBa_M1843 . start_codon 1687 1687 . + 0 ID=annotation2171250;Name=insulin start ;Parent=BBa_M1888 BBa_M1843 . CDS 1687 1698 . + 0 ID=annotation2171252;Name=insulin ;Parent=BBa_M1888 BBa_M1843 . engineered_region 1687 1698 . + 0 ID=annotation2171255;Name=BBa_M1877;Parent=BBa_M1888 BBa_M1843 . engineered_region 1707 5712 . + 0 ID=BBa_M1987;Name=BBa_M1987 BBa_M1843 . CDS 5276 5596 . + 0 ID=BBa_K302001;Name=BBa_K302001;Parent=BBa_M1987 BBa_M1843 . stop_codon 5591 5596 . + 0 ID=annotation2091504;Name=double stop codon;Parent=BBa_K302001 BBa_M1843 . promoter 5605 5712 . + 0 ID=BBa_R0082;Name=BBa_R0082;Parent=BBa_M1987 BBa_M1843 . promoter 5702 5707 . + 0 ID=annotation301167;Name=-10;Parent=BBa_R0082 BBa_M1843 . promoter 5679 5684 . + 0 ID=annotation301166;Name=-35;Parent=BBa_R0082 BBa_M1843 . non_covalent_binding_site 5617 5634 . + 0 ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082 BBa_M1843 . non_covalent_binding_site 5638 5655 . + 0 ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082 BBa_M1843 . non_covalent_binding_site 5658 5675 . + 0 ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082 BBa_M1843 . engineered_region 2004 5269 . + 0 ID=BBa_K123009;Name=BBa_K123009;Parent=BBa_M1987 BBa_M1843 . engineered_region 3528 5269 . + 0 ID=BBa_K123008;Name=BBa_K123008;Parent=BBa_K123009 BBa_M1843 . promoter 3528 3581 . + 0 ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K123008 BBa_M1843 . promoter 3547 3552 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_M1843 . non_covalent_binding_site 3553 3571 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 BBa_M1843 . engineered_region 3528 3581 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_M1843 . promoter 3570 3575 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_M1843 . non_covalent_binding_site 3528 3546 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_M1843 . promoter 3926 3979 . + 0 ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K123008 BBa_M1843 . promoter 3945 3950 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_M1843 . non_covalent_binding_site 3951 3969 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 BBa_M1843 . engineered_region 3926 3979 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_M1843 . promoter 3968 3973 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_M1843 . non_covalent_binding_site 3926 3944 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_M1843 . CDS 3588 3917 . + 0 ID=BBa_K123000;Name=BBa_K123000;Parent=BBa_K123008 BBa_M1843 . CDS 3986 5269 . + 0 ID=BBa_K123001;Name=BBa_K123001;Parent=BBa_K123008 BBa_M1843 . promoter 2754 2807 . + 0 ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K123009 BBa_M1843 . promoter 2773 2778 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_M1843 . non_covalent_binding_site 2779 2797 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 BBa_M1843 . engineered_region 2754 2807 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_M1843 . promoter 2796 2801 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_M1843 . non_covalent_binding_site 2754 2772 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_M1843 . CDS 2814 3519 . + 0 ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K123009 BBa_M1843 . engineered_tag 3495 3519 . + 0 ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010 BBa_M1843 . CDS 2814 3488 . + 0 ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010 BBa_M1843 . promoter 2004 2745 . + 0 ID=BBa_K123002;Name=BBa_K123002;Parent=BBa_K123009 BBa_M1843 . engineered_region 2707 2731 . + 0 ID=annotation1992724;Name=barcode;Parent=BBa_K123002 BBa_M1843 . engineered_region 2004 2040 . + 0 ID=annotation1992720;Name=BBa_I14032;Parent=BBa_K123002 BBa_M1843 . promoter 2006 2011 . + 0 ID=annotation1992718;Name=-35;Parent=BBa_K123002 BBa_M1843 . tag 2667 2700 . + 0 ID=annotation1992722;Name=SsrA;Parent=BBa_K123002 BBa_M1843 . promoter 2029 2034 . + 0 ID=annotation1992719;Name=-10;Parent=BBa_K123002 BBa_M1843 . engineered_region 2047 2706 . + 0 ID=annotation1992723;Name=BBa_C0040;Parent=BBa_K123002 BBa_M1843 . CDS 2050 2666 . + 0 ID=annotation1992721;Name=tetR;Parent=BBa_K123002 BBa_M1843 . non_covalent_binding_site 2046 2060 . + 0 ID=annotation1992733;Name=ERE;Parent=BBa_K123002 BBa_M1843 . operator 2004 2040 . + 0 ID=annotation1992717;Name=P(Lac) IQ;Parent=BBa_K123002 BBa_M1843 . ribosome_entry_site 1707 1722 . + 0 ID=BBa_I742145;Name=BBa_I742145;Parent=BBa_M1987 BBa_M1843 . ribosome_entry_site 1709 1715 . + 0 ID=annotation1955475;Name=rbs;Parent=BBa_I742145 BBa_M1843 . CDS 1729 1995 . + 0 ID=BBa_M1986;Name=BBa_M1986;Parent=BBa_M1987 BBa_M1843 . start_codon 1729 1731 . + 0 ID=annotation2171187;Name=Start codon;Parent=BBa_M1986 BBa_M1843 . CDS 1732 1992 . + 0 ID=annotation2171189;Name=Open reading frame;Parent=BBa_M1986 BBa_M1843 . stop_codon 1993 1995 . + 0 ID=annotation2171188;Name=Stop Codon;Parent=BBa_M1986 BBa_M1843 . sequence_feature 1687 1698 . + 0 ID=BBa_M1877;Name=BBa_M1877 BBa_M1843 . stop_codon 1698 1698 . + 0 ID=annotation2171248;Name=insulin stop ;Parent=BBa_M1877 BBa_M1843 . start_codon 1687 1687 . + 0 ID=annotation2171242;Name=insulin start ;Parent=BBa_M1877 BBa_M1843 . CDS 1687 1698 . + 0 ID=annotation2171241;Name=coding sequence ;Parent=BBa_M1877 BBa_M1843 . CDS 1687 1698 . + 0 ID=annotation2171243;Name=insulin ;Parent=BBa_M1877 BBa_M1843 . CDS 2814 3494 . + 0 ID=BBa_E1010;Name=BBa_E1010 BBa_M1843 . engineered_tag 3495 3519 . + 0 ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010 BBa_M1843 . CDS 2814 3488 . + 0 ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010 BBa_M1843 . sequence_feature 1 717 . + 0 ID=BBa_M1872;Name=BBa_M1872 BBa_M1843 . start_codon 1 299 . + 0 ID=annotation2171235;Name=start;Parent=BBa_M1872 BBa_M1843 . operator 300 500 . + 0 ID=annotation2171236;Name=operator;Parent=BBa_M1872 BBa_M1843 . stop_codon 1 200 . + 0 ID=annotation2171237;Name=stop;Parent=BBa_M1872 BBa_M1843 . engineered_region 1 717 . + 0 ID=BBa_M1845;Name=BBa_M1845 BBa_M1843 . stop_codon 1 200 . + 0 ID=annotation2171245;Name=stop;Parent=BBa_M1845 BBa_M1843 . operator 300 500 . + 0 ID=annotation2171246;Name=operator;Parent=BBa_M1845 BBa_M1843 . engineered_region 1 717 . + 0 ID=annotation2171247;Name=BBa_M1872;Parent=BBa_M1845 BBa_M1843 . start_codon 1 299 . + 0 ID=annotation2171244;Name=start;Parent=BBa_M1845 >BBa_M11107 atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgt ttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaa gaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagacc ctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactgg ccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggc catcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatc gctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggcca cggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcat atcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctga >BBa_M1843 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccaca agttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaa gctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatg aagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggca actacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaa ggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcag aagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagc agaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaaga ccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctg tacaagtactagagccatagcacctatactagagtccctatcagtgatagagattgacatccctatcagtgatagagat actgagcactactagagaaagaggagaaatactagatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaa cctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtc aagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatcc aggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaaccca gagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgcc aagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagt cggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggc tgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaag atggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggt agtgccctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcag ctgtctcttatacacatcttactagagccatagcacctatactagagacggagaagcagcgaatactagatggccaaca ccgtgtcggccaagaagatgacccgcaagatcgccaagcgtacggcgatcaaccgctcgcgccgttcgcggatgcgtac cttcgtccgcaaggtcgaggaggcgattgcgtccggcgatcagggccaggccctcaccgccctccgcgccgccgagccg gagatcatgcgcgctgcccagaacggcatcgttcacaagaacaatgcctcgcggaaggtttcccgcctggccgcccgcg tgaaggccatcgcggcctgatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcaga gtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtt taacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggcttt gctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagat tttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacac ggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgc attatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaa gaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcag agccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgc aaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtccctatcagtg atagagattgacatccctatcagtgatagagatactgagcactactagatggcttcctccgaagacgttatcaaagagt tcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtcc gtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccg cagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaag gtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacgg tgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggt tgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaag acggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaac cgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcac tccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtccctatcagtgatagagattgacatcc ctatcagtgatagagatactgagcactactagatggccggccctcatatccaagtgactacccgcgatggggagatacg cgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgc ggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccg acgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccga actggatggccttaagctcacgatcccgccggactccaccggttaatactagagtccctatcagtgatagagattgaca tccctatcagtgatagagatactgagcactactagatggccggcaaccctcagacactgccggtctttccagacctcga catcttttcgcccgaatatgcctgcaaccgcgaaaaatacgcagcccgcgcccttagggactatccgctgcacttctat aagccgctgaatttgtggatcgtcagcaagcacaaggatgtgcgctcggccttgttcactccgcaggtcttttcttctg tcgcgtttggcctgctccccccgcccgatgatatcgcgccgcgcgtgccggacctttacaccgacgtgcatctgccctc gatggacccgccggagcacaccaagctcagggttcccgtccagcaagccctcctccccggtcggctggtgggtaaggac gaggtcgttcgccggatcgccaacgaacttatcgataccttcatcgacaagggcgaatgcgatctcctccacgatttca gctacaaacttgcgctctacttgatcgtcgatctgctcgggatccccaaggaacgggcggaagattatcatcgctggtc caactgcttcttccagctgttcacccctaaggtaccggagcgcgccgacgcaagattcttcgtgccgatgccggaagag gtactgcggcagaactgggaaggcctggcggaggcgaacgactacctgcgcgaagtcgtcgagaaccttgatcgcaatc ccggcaacaacatgctgtcaaacctgttgcagctgcgcgaaccggacggctcacgcacgatcacgatcagcgccaatgt ttgcaacgcactggaatttggggcggctggtcatgacaccacggcaacgctgatcgcgcacctcacctatttcgtgctg accacgccggacctcaaggacactcttaccgaggatccgtcgctgatccccgcggcgatttccgaaacgctgcgccgtc gctgtccggtcgacgggctgttccggcgaacgctgtcggatgtagaactatgcggccagaaaatcgaatccggatcgat cgtctacctcgatctcaccgctgcaaatcttgaccccgatgtattccccgaacctgagacgtttcgcctgaaccgcgac aatatcaaggaaatggtctctttcggcttcggtcgtcacgtctgcgcaggacagtacctgtcccgtatcgaagcaaaag cggcctacgaagaactgatgcgccgtattcccaacatgcgtctcgctgatggtttcaagctggaatacatgccttccgt ggcgacgacggttctcaaggggcttccgctggtctgggacaaaaacaccggttaatactagatgatcatgagtaaagaa tctattatttttgtcggtctgtttacagtgattttgagcgcggttatccttatgctgtcatatacaagcagcggccaag agcttaatcagtatgttaaaatagaagtccagcaaggcgacacactctggtcaattgctgatcaggtagccgatacaaa aaagataaacaaaaatgattttattgaatgggtagctgataaaaatcaacttcaaacctctgatatccagccgggtgat gagttagtgatcccattgaaaaagaagcatcaggatgcatatgaattagcaactgtaagataataatactagagtccct tgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattatt ctgcatttttggggagaatggact