LOCUS BBa_M1844 6890 bp DNA linear 22-Nov-2024 DEFINITION RPS Tgene from Methilobacteriun chloromeneticum ACCESSION BBa_M1844 VERSION BBa_M1844.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 misc_feature 1..37 /label=BBa_I14032 CDS 47..663 /label=tetR misc_feature 664..697 /label=SsrA misc_feature 44..703 /label=BBa_C0040 misc_feature 704..728 /label=barcode misc_feature 43..57 /label=ERE promoter 1..742 /label=BBa_K123002 misc_feature 755..758 /label=conserved RBS 751..762 /label=BBa_B0034 misc_feature 769..771 /label=Start codon misc_feature 1033..1035 /label=Stop Codon CDS 772..1032 /label=Open reading frame CDS 769..1035 /label=BBa_M1986 misc_feature 1042..1340 /label=start misc_feature 1341..1541 /label=operator misc_feature 1042..1241 /label=stop misc_feature 1042..1758 /label=BBa_M1872 misc_feature 1042..1340 /label=start misc_feature 1042..1241 /label=stop misc_feature 1341..1541 /label=operator misc_feature 1042..1758 /label=BBa_M1872 misc_feature 1042..1758 /label=BBa_M1845 CDS 1767..1778 /label=coding sequence misc_feature 1767..1767 /label=insulin start CDS 1767..1778 /label=insulin misc_feature 1778..1778 /label=insulin stop misc_feature 1767..1778 /label=BBa_M1877 misc_feature 1787..1805 /label=TetR 1 misc_feature 1787..1840 /label=BBa_R0040 promoter 1806..1811 /label=-35 misc_feature 1812..1830 /label=TetR 2 promoter 1829..1834 /label=-10 promoter 1787..1840 /label=BBa_R0040 misc_feature 1853..1856 /label=conserved RBS 1849..1860 /label=BBa_B0034 CDS 1867..2552 /label=cds misc_feature 2246..2266 /label=just for fun misc_feature 1867..1869 /label=start codon misc_feature 1867..2552 /label=BBa_M1998 stem_loop 2572..2615 /label=stem_loop misc_feature 2561..2640 /label=BBa_B0010 terminator 2561..2640 /label=BBa_B0010 stem_loop 2656..2675 /label=T7 TE misc_feature 2682..2682 /label=stop polyA_site 2676..2689 /label=polya misc_feature 2649..2689 /label=BBa_B0012 terminator 2649..2689 /label=BBa_B0012 misc_feature 1787..2689 /label=BBa_M1999 misc_feature 2698..2703 /label=PvuII cut site misc_feature 2701..2719 /label=Mosaic End (forward) misc_feature 2698..2719 /label=BBa_M1000 misc_feature 1767..2719 /label=BBa_M1887 CDS 2728..2739 /label=coding sequence misc_feature 2728..2728 /label=insulin start CDS 2728..2739 /label=insulin misc_feature 2739..2739 /label=insulin stop misc_feature 2728..2739 /label=BBa_M1877 misc_feature 2728..2728 /label=insulin start CDS 2728..2739 /label=coding sequence CDS 2728..2739 /label=insulin misc_feature 2739..2739 /label=insulin stop misc_feature 2728..2739 /label=BBa_M1877 misc_feature 2728..2739 /label=BBa_M1888 CDS 3855..4529 /label=mrfp1 misc_feature 4536..4560 /label=Help:Barcodes CDS 3855..4535 /label=BBa_E1010 RBS 2750..2756 /label=rbs RBS 2748..2763 /label=BBa_I742145 misc_feature 2770..2772 /label=Start codon misc_feature 3034..3036 /label=Stop Codon CDS 2773..3033 /label=Open reading frame CDS 2770..3036 /label=BBa_M1986 misc_feature 3045..3081 /label=P(Lac) IQ promoter 3047..3052 /label=-35 promoter 3070..3075 /label=-10 misc_feature 3045..3081 /label=BBa_I14032 CDS 3091..3707 /label=tetR misc_feature 3708..3741 /label=SsrA misc_feature 3088..3747 /label=BBa_C0040 misc_feature 3748..3772 /label=barcode misc_feature 3087..3101 /label=ERE promoter 3045..3786 /label=BBa_K123002 misc_feature 3795..3813 /label=TetR 1 misc_feature 3795..3848 /label=BBa_R0040 promoter 3814..3819 /label=-35 misc_feature 3820..3838 /label=TetR 2 promoter 3837..3842 /label=-10 promoter 3795..3848 /label=BBa_R0040 CDS 3855..4529 /label=mrfp1 misc_feature 4536..4560 /label=Help:Barcodes CDS 3855..4560 /label=BBa_E1010 misc_feature 4569..4587 /label=TetR 1 misc_feature 4569..4622 /label=BBa_R0040 promoter 4588..4593 /label=-35 misc_feature 4594..4612 /label=TetR 2 promoter 4611..4616 /label=-10 promoter 4569..4622 /label=BBa_R0040 CDS 4629..4958 /label=BBa_K123000 misc_feature 4967..4985 /label=TetR 1 misc_feature 4967..5020 /label=BBa_R0040 promoter 4986..4991 /label=-35 misc_feature 4992..5010 /label=TetR 2 promoter 5009..5014 /label=-10 promoter 4967..5020 /label=BBa_R0040 CDS 5027..6310 /label=BBa_K123001 misc_feature 4569..6310 /label=BBa_K123008 misc_feature 3045..6310 /label=BBa_K123009 misc_feature 6632..6637 /label=double stop codon CDS 6317..6637 /label=BBa_K302001 misc_feature 6658..6675 /label=C1 OmpR misc_feature 6679..6696 /label=C2 OmpR misc_feature 6699..6716 /label=C3 OmpR promoter 6720..6725 /label=-35 promoter 6743..6748 /label=-10 promoter 6646..6753 /label=BBa_R0082 misc_feature 2748..6753 /label=BBa_M1987 misc_feature 1042..6753 /label=BBa_M1843 stem_loop 6773..6816 /label=stem_loop misc_feature 6762..6841 /label=BBa_B0010 terminator 6762..6841 /label=BBa_B0010 stem_loop 6857..6876 /label=T7 TE misc_feature 6883..6883 /label=stop polyA_site 6877..6890 /label=polya misc_feature 6850..6890 /label=BBa_B0012 terminator 6850..6890 /label=BBa_B0012 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagaggtcag agtgaccatg 61 tccagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga 121 atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg 181 tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat 241 aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat 301 aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta 361 ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc 421 caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact 481 ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct 541 actactgata gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt 601 gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt 661 aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac 721 tgatagtgct agtgtagatc actactagag aaagaggaga aatactagat ggccaacacc 781 gtgtcggcca agaagatgac ccgcaagatc gccaagcgta cggcgatcaa ccgctcgcgc 841 cgttcgcgga tgcgtacctt cgtccgcaag gtcgaggagg cgattgcgtc cggcgatcag 901 ggccaggccc tcaccgccct ccgcgccgcc gagccggaga tcatgcgcgc tgcccagaac 961 ggcatcgttc acaagaacaa tgcctcgcgg aaggtttccc gcctggccgc ccgcgtgaag 1021 gccatcgcgg cctgatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg 1081 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 1141 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 1201 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc 1261 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 1321 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 1381 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 1441 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 1501 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 1561 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 1621 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac 1681 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 1741 atggacgagc tgtacaagta ctagagccat agcacctata ctagagtccc tatcagtgat 1801 agagattgac atccctatca gtgatagaga tactgagcac tactagagaa agaggagaaa 1861 tactagatga tcctcacccc ggaacaagtt gcagcagcgc aaaaggccaa cctcgaaacg 1921 ctgttcggcc tgaccaccaa ggcgtttgaa ggcgtcgaaa agctcgtcga gctgaacctg 1981 caggtcgtca agacttcgtt cgcagaaggc gttgacaacg ccaagaaggc gctgtcggcc 2041 aaggacgcac aggaactgct ggccatccag gccgcagccg tgcagccggt tgccgaaaag 2101 accctggcct acacccgcca cctgtatgaa atcgcttcgg aaacccagag cgagttcacc 2161 aaggtagccg aggctcaact ggccgaaggc tcgaagaacg tgcaagcgct ggtcgagaac 2221 ctcgccaaga acgccccggc cggttcggaa tcgaccgtgg ccatcgtgaa gtcggcgatc 2281 tccgctgcca acaacgccta cgagtcggtg cagaaggcga ccaagcaagc ggtcgaaatc 2341 gctgaaacca acttccaggc tgcggctacg gctgccacca aggctgccca gcaagccagc 2401 gccacggccc gtacggccac ggcaaagaag acgacggctg cctgataact gcctgcgttg 2461 aagatggacc ggctgcggcc ggtccgttgg caaagcatat cgacgcctgg cgtttgcggt 2521 gtgttttgcc aacgatgaag gtagtgccct gatactagag ccaggcatca aataaaacga 2581 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 2641 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagcag 2701 ctgtctctta tacacatctt actagagcca tagcacctat actagagacg gagaagcagc 2761 gaatactaga tggccaacac cgtgtcggcc aagaagatga cccgcaagat cgccaagcgt 2821 acggcgatca accgctcgcg ccgttcgcgg atgcgtacct tcgtccgcaa ggtcgaggag 2881 gcgattgcgt ccggcgatca gggccaggcc ctcaccgccc tccgcgccgc cgagccggag 2941 atcatgcgcg ctgcccagaa cggcatcgtt cacaagaaca atgcctcgcg gaaggtttcc 3001 cgcctggccg cccgcgtgaa ggccatcgcg gcctgatact agagtggtgc aaaacctttc 3061 gcggtatggc atgatagcgc ctactagagg tcagagtgac catgtccaga ttagataaaa 3121 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa 3181 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa 3241 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact 3301 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta 3361 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag 3421 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac 3481 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg 3541 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc 3601 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct 3661 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt 3721 ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag tgctagtgta 3781 gatcactact agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata 3841 ctgagcacta ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag 3901 ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc 3961 gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt 4021 tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc 4081 cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg 4141 ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg 4201 gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta 4261 tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg 4321 ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg 4381 aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg 4441 acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac 4501 gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc 4561 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc 4621 actactagat ggccggccct catatccaag tgactacccg cgatggggag atacgcgaac 4681 tcgacgtcgc agcctccggg ttcctgatgg aagcccttcg cgacgccaat atcgacggcg 4741 tcgaggcgat gtgcggcgga tgctgctcct gcgcgacctg ccacgtctac atcgacgctg 4801 ctcccgccgg gaccctgccg ccggtctcct ccgacgagga gatgctgctt tccggcctgg 4861 tctcgacccc cggacggtcg cggctctcct gccaaattcc ggtcacggcc gaactggatg 4921 gccttaagct cacgatcccg ccggactcca ccggttaata ctagagtccc tatcagtgat 4981 agagattgac atccctatca gtgatagaga tactgagcac tactagatgg ccggcaaccc 5041 tcagacactg ccggtctttc cagacctcga catcttttcg cccgaatatg cctgcaaccg 5101 cgaaaaatac gcagcccgcg cccttaggga ctatccgctg cacttctata agccgctgaa 5161 tttgtggatc gtcagcaagc acaaggatgt gcgctcggcc ttgttcactc cgcaggtctt 5221 ttcttctgtc gcgtttggcc tgctcccccc gcccgatgat atcgcgccgc gcgtgccgga 5281 cctttacacc gacgtgcatc tgccctcgat ggacccgccg gagcacacca agctcagggt 5341 tcccgtccag caagccctcc tccccggtcg gctggtgggt aaggacgagg tcgttcgccg 5401 gatcgccaac gaacttatcg ataccttcat cgacaagggc gaatgcgatc tcctccacga 5461 tttcagctac aaacttgcgc tctacttgat cgtcgatctg ctcgggatcc ccaaggaacg 5521 ggcggaagat tatcatcgct ggtccaactg cttcttccag ctgttcaccc ctaaggtacc 5581 ggagcgcgcc gacgcaagat tcttcgtgcc gatgccggaa gaggtactgc ggcagaactg 5641 ggaaggcctg gcggaggcga acgactacct gcgcgaagtc gtcgagaacc ttgatcgcaa 5701 tcccggcaac aacatgctgt caaacctgtt gcagctgcgc gaaccggacg gctcacgcac 5761 gatcacgatc agcgccaatg tttgcaacgc actggaattt ggggcggctg gtcatgacac 5821 cacggcaacg ctgatcgcgc acctcaccta tttcgtgctg accacgccgg acctcaagga 5881 cactcttacc gaggatccgt cgctgatccc cgcggcgatt tccgaaacgc tgcgccgtcg 5941 ctgtccggtc gacgggctgt tccggcgaac gctgtcggat gtagaactat gcggccagaa 6001 aatcgaatcc ggatcgatcg tctacctcga tctcaccgct gcaaatcttg accccgatgt 6061 attccccgaa cctgagacgt ttcgcctgaa ccgcgacaat atcaaggaaa tggtctcttt 6121 cggcttcggt cgtcacgtct gcgcaggaca gtacctgtcc cgtatcgaag caaaagcggc 6181 ctacgaagaa ctgatgcgcc gtattcccaa catgcgtctc gctgatggtt tcaagctgga 6241 atacatgcct tccgtggcga cgacggttct caaggggctt ccgctggtct gggacaaaaa 6301 caccggttaa tactagatga tcatgagtaa agaatctatt atttttgtcg gtctgtttac 6361 agtgattttg agcgcggtta tccttatgct gtcatataca agcagcggcc aagagcttaa 6421 tcagtatgtt aaaatagaag tccagcaagg cgacacactc tggtcaattg ctgatcaggt 6481 agccgataca aaaaagataa acaaaaatga ttttattgaa tgggtagctg ataaaaatca 6541 acttcaaacc tctgatatcc agccgggtga tgagttagtg atcccattga aaaagaagca 6601 tcaggatgca tatgaattag caactgtaag ataataatac tagagtccct tgcatttaca 6661 ttttgaaaca tctatagcga taaatgaaac atcttaaaag ttttagtatc atattcgtgt 6721 tggattattc tgcatttttg gggagaatgg acttactaga gccaggcatc aaataaaacg 6781 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 6841 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata // LOCUS BBa_M11107 686 bp DNA linear 22-Nov-2024 DEFINITION example ACCESSION BBa_M11107 VERSION BBa_M11107.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=start ATG ORIGIN 1 atgatcctca ccccggaaca agttgcagca gcgcaaaagg ccaacctcga aacgctgttc 61 ggcctgacca ccaaggcgtt tgaaggcgtc gaaaagctcg tcgagctgaa cctgcaggtc 121 gtcaagactt cgttcgcaga aggcgttgac aacgccaaga aggcgctgtc ggccaaggac 181 gcacaggaac tgctggccat ccaggccgca gccgtgcagc cggttgccga aaagaccctg 241 gcctacaccc gccacctgta tgaaatcgct tcggaaaccc agagcgagtt caccaaggta 301 gccgaggctc aactggccga aggctcgaag aacgtgcaag cgctggtcga gaacctcgcc 361 aagaacgccc cggccggttc ggaatcgacc gtggccatcg tgaagtcggc gatctccgct 421 gccaacaacg cctacgagtc ggtgcagaag gcgaccaagc aagcggtcga aatcgctgaa 481 accaacttcc aggctgcggc tacggctgcc accaaggctg cccagcaagc cagcgccacg 541 gcccgtacgg ccacggcaaa gaagacgacg gctgcctgat aactgcctgc gttgaagatg 601 gaccggctgc ggccggtccg ttggcaaagc atatcgacgc ctggcgtttg cggtgtgttt 661 tgccaacgat gaaggtagtg ccctga //