BBa_K123009 1 BBa_K123009 lacIq->BisdB 2008-10-28T12:00:00Z 2015-05-08T01:09:44Z Bisphenolics This Construct will allow for the detection and degredation of BPA in cells already containing the ER (K123003) biobrick. It works in two systems first. Together K123003 and K123009 produce a NOT gate where when Bisphenol A (BPA), Estrogen or any other estrogen mimic is in the system the product of K123003 dimerizes and attaches to the ERE site of K123009. This stops the production of TetR (R0040) by phsically stoping the Polymerase from transcribing it. This allows the pTet(C0040)promoter to turn on the production of RFP (E1010) for detection as well as BisdA(K123000) and BisdB(K123001)which allow for the degredation of BPA. false false _241_ 0 2742 9 It's complicated true While BisdA and BisdB have only been shown to degrade BPA we have put in RFP to allow for the detection of other estrogen mimics as well. true Jason Gardiner component2267219 1 BBa_K123002 component2267220 1 BBa_R0040 component2267227 1 BBa_E1010 component2267240 1 BBa_K123008 annotation2267227 1 BBa_E1010 range2267227 1 811 1516 annotation2267219 1 BBa_K123002 range2267219 1 1 742 annotation2267220 1 BBa_R0040 range2267220 1 751 804 annotation2267240 1 BBa_K123008 range2267240 1 1525 3266 BBa_K123001 1 BisdB BisdB 2008-10-27T12:00:00Z 2015-05-08T01:09:44Z Sphingomonas sp. strain AO1 When used in conjunction with BisdA this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli by Miho Sasaki1 et al. 2005. Miho Sasaki1, Jun-ichi Maki, Ko-ichi Oshiman, Yoshinobu Matsumura1, and Tetsuaki Tsuchido.2005, Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1. Biodegredation 16: 449-459 false false _241_ 0 2742 9 It's complicated true When used with BisdA the degredation of Bisphenol A sharply increases true Jason Gardiner BBa_M1844 1 BBa_M1844 RPS Tgene from Methilobacteriun chloromeneticum 2012-03-27T11:00:00Z 2015-05-08T01:13:58Z E. coli Composite part that induces post translational inhibition of arginine and ornithine decarboxylase true false _768_ 0 12257 9 Discontinued false undefined false sarah guzman component2171569 1 BBa_B0034 component2171573 1 BBa_M1986 component2171656 1 BBa_B0010 component2171658 1 BBa_B0012 component2171567 1 BBa_K123002 component2171655 1 BBa_M1843 annotation2171658 1 BBa_B0012 range2171658 1 6850 6890 annotation2171656 1 BBa_B0010 range2171656 1 6762 6841 annotation2171655 1 BBa_M1843 range2171655 1 1042 6753 annotation2171569 1 BBa_B0034 range2171569 1 751 762 annotation2171567 1 BBa_K123002 range2171567 1 1 742 annotation2171573 1 BBa_M1986 range2171573 1 769 1035 BBa_K123002 1 BBa_K123002 LacIQ ERE TetR 2008-10-27T12:00:00Z 2015-05-08T01:09:44Z The Estrogen Responsive Element was taken from the Human Estrogen Responsive Element This composite part contains LacIQ (BBa_I14032) and Estrogen Receptor Element (ERE) and TetR (BBa_C0040). The Estrogen Responsive element is to be used in conjunction with ER (Estrogen Receptor). This design involves ER binding to the ERE and disrupting the constituative promoter LacIQ from producing TetR via physically getting in the way of the polymerase. false false _241_ 0 2742 9 It's complicated true There is some data that suggests the ER is toxic to the cell. However in our experiments while we did see a decrease in growth rate we did not find ER to be fatal to the cell. true Jason Gardiner annotation1992722 1 SsrA range1992722 1 664 697 annotation1992718 1 -35 range1992718 1 3 8 annotation1992733 1 ERE range1992733 1 43 57 annotation1992724 1 barcode range1992724 1 704 728 annotation1992719 1 -10 range1992719 1 26 31 annotation1992721 1 tetR range1992721 1 47 663 annotation1992720 1 BBa_I14032 range1992720 1 1 37 annotation1992717 1 P(Lac) IQ range1992717 1 1 37 annotation1992723 1 BBa_C0040 range1992723 1 44 703 BBa_K123000 1 BisdA BisdA 2008-10-27T12:00:00Z 2015-05-08T01:09:44Z Sphingomonas sp. strain AO1 When used in conjunction with BisdB this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli by Miho Sasaki1 et al. 2005. Miho Sasaki1, Jun-ichi Maki, Ko-ichi Oshiman, Yoshinobu Matsumura1, and Tetsuaki Tsuchido.2005, Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1. Biodegredation 16: 449-459 false false _241_ 0 2742 9 It's complicated true These parts where designed to include a his tag to make it easier to view enzyme presence true Jason Gardiner BBa_I742145 1 BBa_I742145 sam5 native rbs 2007-10-23T11:00:00Z 2015-08-31T04:08:03Z Saccharothrix espanaensis DSM 44229 This is the native ribosome binding site for sam5 (4-coumarate-3-hydroxylase) of the bacterium Saccharothrix espanaensis. false false _123_ 0 837 163 Not in stock false This is a virtual part to be added to coding sequence I742141. false Chris French annotation1955475 1 rbs range1955475 1 3 9 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_M1999 1 BBa_M1999 composite 2012-03-21T12:00:00Z 2015-05-08T01:13:58Z blah blah false false _768_ 0 12223 9 Not in stock false blah false David Harris component2170880 1 BBa_B0034 component2170885 1 BBa_B0010 component2170884 1 BBa_M1998 component2170887 1 BBa_B0012 component2170874 1 BBa_R0040 annotation2170884 1 BBa_M1998 range2170884 1 81 766 annotation2170885 1 BBa_B0010 range2170885 1 775 854 annotation2170874 1 BBa_R0040 range2170874 1 1 54 annotation2170880 1 BBa_B0034 range2170880 1 63 74 annotation2170887 1 BBa_B0012 range2170887 1 863 903 BBa_M1998 1 BBa_M1998 blah 2012-03-21T12:00:00Z 2015-05-08T01:13:58Z Baccilus blah blah blah blah true false _768_ 0 12223 9 Discontinued false blah blah blah false David Harris annotation2170793 1 just for fun range2170793 1 380 400 annotation2170787 1 cds range2170787 1 1 686 annotation2170800 1 start codon range2170800 1 1 3 BBa_K302001 1 BBa_K302001 yneA coding sequence 2010-08-11T11:00:00Z 2015-05-08T01:11:52Z Genomic DNA ''Bacillus subtilis'' ''Bacillus subtilis'' in response to stress such as DNA damage stops the cells from dividing. This is a part of the SOS response initiated by the accumulation of single stranded DNA from DNA damage or stalled replication. Two proteins are vital for this response: RecA and LexA. RecA forms filaments on ssDNA and promotes the autocleavage of LexA. LexA usually represses the SOS operon. ''dinR'' is homologous to ''lexA'' in ''E. coli'' and is transcribed in the opposite direction of ''yneA''. ''yneA'' stops the formation of ''ftsZ'' ring indirectly. When ''ftsZ'' forms a 30 subunit ring at the midpoint of the cell, it will contract and cause cell division. By expressing ''yneA'' and inhibiting ''ftsZ'' ring formation, the cells will grow filamentous. By inhibiting cell division, yneA allows the DNA damage genes to repair the DNA damage before continuing with the cell division cycle. It is hypothesized that yneA acts through an unknown transmembrane protein to inhibit ftsZ ring formation; we call this/these unknown components ???Blackbox proteins???. As the evidence shows expression of yneA leads to filamentation. false false _442_ 0 6014 9 Not in stock false None. false Rachel May annotation2091504 1 double stop codon range2091504 1 316 321 BBa_M1888 1 BBa_M1888 fdf 2012-03-26T11:00:00Z 2015-05-08T01:13:58Z dfdf dfdf true false _768_ 0 12283 9 Discontinued false dfdf false Elizabeth Martinez annotation2171250 1 insulin start range2171250 1 1 1 annotation2171251 1 coding sequence range2171251 1 1 12 annotation2171252 1 insulin range2171252 1 1 12 annotation2171255 1 BBa_M1877 range2171255 1 1 12 annotation2171254 1 insulin stop range2171254 1 12 12 BBa_M1877 1 BBa_M1877 human insulin 2012-03-26T11:00:00Z 2015-05-08T01:13:58Z dfadf zedfd false true _768_ 0 12283 9 Not in stock false dfdf false Elizabeth Martinez annotation2171241 1 coding sequence range2171241 1 1 12 annotation2171242 1 insulin start range2171242 1 1 1 annotation2171243 1 insulin range2171243 1 1 12 annotation2171248 1 insulin stop range2171248 1 12 12 BBa_M1845 1 BBa_M1845 His6 2012-03-26T11:00:00Z 2015-05-08T01:13:58Z Hus6 His6 true false _768_ 0 12257 9 Discontinued false catcatcaccatcaccac false sarah guzman annotation2171244 1 start range2171244 1 1 299 annotation2171245 1 stop range2171245 1 1 200 annotation2171246 1 operator range2171246 1 300 500 annotation2171247 1 BBa_M1872 range2171247 1 1 717 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_M1872 1 BBa_M1872 GFP 2012-03-26T11:00:00Z 2015-05-08T01:13:58Z Ampicilin Ampicilin true false _768_ 0 12257 9 Discontinued false Ampicillin: atgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaac gctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaaga tccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcc cgtgttgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcac agaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggcca acttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgcctt gatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgcagcaatggcaacaac gttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaag ttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtct cgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaac tatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaa false sarah guzman annotation2171237 1 stop range2171237 1 1 200 annotation2171235 1 start range2171235 1 1 299 annotation2171236 1 operator range2171236 1 300 500 BBa_M1843 1 BBa_M1843 His 2012-03-26T11:00:00Z 2015-05-08T01:13:58Z His His true false _768_ 0 12257 9 Discontinued false His false sarah guzman component2171287 1 BBa_M1845 component2171317 1 BBa_M1888 component2171363 1 BBa_M1987 component2171313 1 BBa_M1887 component2171341 1 BBa_E1010 component2171316 1 BBa_M1877 component2171285 1 BBa_M1872 annotation2171316 1 BBa_M1877 range2171316 1 1687 1698 annotation2171363 1 BBa_M1987 range2171363 1 1707 5712 annotation2171341 1 BBa_E1010 range2171341 1 2814 3494 annotation2171285 1 BBa_M1872 range2171285 1 1 717 annotation2171317 1 BBa_M1888 range2171317 1 1687 1698 annotation2171313 1 BBa_M1887 range2171313 1 726 1678 annotation2171287 1 BBa_M1845 range2171287 1 1 717 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986785 1 -35 range1986785 1 20 25 annotation1986787 1 -10 range1986787 1 43 48 BBa_M1987 1 BBa_M1987 Composite with rpsT gene 2012-03-26T11:00:00Z 2015-05-08T01:13:58Z the source of the gene is from Methylobacterium chloromethanicum. Other parts are from the registry. the rpsT gene with a promoter-RBS- and two TC stops added false false _768_ 0 12223 9 Not in stock false I just found different ribosome binding sites and Tc stops from the registry. The promoter is for the glucose controlled ompC porin gene. The RBS is the native rbs for the sam5 gene of Saccharothrix espanaensis The first stop stops RNA polymerase from transcribing the TetR gene. The second codes for a gene that inhibits cell division false David Harris component2295770 1 BBa_K123009 component2295738 1 BBa_M1986 component2295778 1 BBa_R0082 component2295772 1 BBa_K302001 component2295734 1 BBa_I742145 annotation2295738 1 BBa_M1986 range2295738 1 23 289 annotation2295778 1 BBa_R0082 range2295778 1 3899 4006 annotation2295772 1 BBa_K302001 range2295772 1 3570 3890 annotation2295770 1 BBa_K123009 range2295770 1 298 3563 annotation2295734 1 BBa_I742145 range2295734 1 1 16 BBa_R0082 1 OmpR Promoter (OmpR, positive) 2004-01-27T12:00:00Z 2015-05-08T01:14:15Z NC_000193 E. coli K12 Released HQ 2013 Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. false false _1_ 0 24 7 In stock false The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown. true Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004) annotation301154 1 C1 OmpR range301154 1 13 30 annotation301155 1 C2 OmpR range301155 1 34 51 annotation301156 1 C3 OmpR range301156 1 54 71 annotation301167 1 -10 range301167 1 98 103 annotation301166 1 -35 range301166 1 75 80 BBa_M11107 1 BBa_M11107 example 2010-04-01T11:00:00Z 2015-05-08T01:13:53Z exampl example false true _302_ 0 2425 303 Not in stock false exampl false Charles Miller annotation2067582 1 start ATG range2067582 1 1 3 BBa_M1986 1 BBa_M1986 rpsT gene from Methylobacterium Chloromethanicum 2012-03-26T11:00:00Z 2015-05-08T01:13:58Z Methylobacterium Chloromethanicum. The sequence comes from the site http://www.ncbi.nlm.nih.gov/nuccore/218528082?from=359&to=625 The rpsT gene binds directly to the 16s rRNA and induces post translational inhibition of arginine and ornithine decarboxylase false false _768_ 0 12223 9 Not in stock false I copied and pasted the sequence from NCBI. false David Harris annotation2171189 1 Open reading frame range2171189 1 4 264 annotation2171188 1 Stop Codon range2171188 1 265 267 annotation2171187 1 Start codon range2171187 1 1 3 BBa_K123008 1 BBa_K123008 BisdA+BisdB 2008-10-27T12:00:00Z 2015-05-08T01:09:44Z Bisphenolics This part when used will allow the production of TetR to increase the production of BisdA and BisdB. When produced BisdA and BisdB will cause the degredation of Bisphenol A. false false _241_ 0 2742 9 It's complicated false N/A false Jason Gardiner component1992762 1 BBa_R0040 component1992756 1 BBa_R0040 component1992767 1 BBa_K123001 component1992761 1 BBa_K123000 annotation1992762 1 BBa_R0040 range1992762 1 399 452 annotation1992761 1 BBa_K123000 range1992761 1 61 390 annotation1992767 1 BBa_K123001 range1992767 1 459 1742 annotation1992756 1 BBa_R0040 range1992756 1 1 54 BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation1014044 1 mrfp1 range1014044 1 1 675 annotation2214014 1 Help:Barcodes range2214014 1 682 706 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_M1000 1 BBa_M1000 Left mosaic end for Tn5 transpososome 2006-03-16T12:00:00Z 2015-05-08T01:13:51Z Synthesis from overlapping oligos Left end of a linear transpososome sequence containing the 19 bp mosaic end in the forward orientation. The sequence also contains a PvuII cut site which releases the transpososome when cut with PvuII. Constructed compound parts intended for transposition must not contain other PvuII end, or be PCR amplified with blunt end producing enzymes (Pfu, Phusion) at the PvuII cut site between CAG and CTG. This part, and the companion part M1001 provide Biobrick tools to construct Tn5 transpososomes: linear DNA sequences which can be cut in vitro from a plasmid backbone, or created by PCR, combined in vitro with purified Tn5 trasposase protein, electroportated into cells, and finally randomly inserting into the bacterial chromosome. The parts assembled between the two mosaic ends can be delivered, including antibiotic resistance markers or other functional chromosomal insertions. true false _41_48_1_ 0 6 48 Discontinued false ;biobrick left end, TA cloning overhang GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ;biobrick right end, TA cloning overhang GTT TCT TCC TGC AGC GGC CGC TAC TAG TA ;mosaic end with cag upstream to insert PvuII cut site cagCTGTCTCTTATACACATCT ;mosaic end reverse complement with ctg upstream to insert PvuII cut site AGATGTGTATAAGAGACAGctg ;mosaic left end in biobrick form construction primer F GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG cagCTGTCTCTTATACACATCT, MEL-F ;mosaic left end in biobrick form construction primer R GTT TCT TCC TGC AGC GGC CGC TAC TAG TA AGATGTGTATAAGAGACAGctg, MEL-R ;mosaic right end in biobrick form construction primer F GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG AGATGTGTATAAGAGACAGctg, MER-F ;mosaic right end in biobrick form construction primer R GTT TCT TCC TGC AGC GGC CGC TAC TAG TA cagCTGTCTCTTATACACATCT, MER-R true Tom Knight annotation1825568 1 PvuII cut site range1825568 1 1 6 annotation1825569 1 Mosaic End (forward) range1825569 1 4 22 BBa_M1887 1 BBa_M1887 fgf 2012-03-26T11:00:00Z 2015-05-08T01:13:58Z fgf gfgf true false _768_ 0 12283 9 Discontinued false fgfg false Elizabeth Martinez component2171261 1 BBa_M1877 component2171279 1 BBa_M1999 component2171282 1 BBa_M1000 annotation2171282 1 BBa_M1000 range2171282 1 932 953 annotation2171261 1 BBa_M1877 range2171261 1 1 12 annotation2171279 1 BBa_M1999 range2171279 1 21 923 BBa_M1000_sequence 1 cagctgtctcttatacacatct BBa_M1998_sequence 1 atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctga BBa_R0082_sequence 1 tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact BBa_K123000_sequence 1 atggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaa BBa_M1999_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_M1872_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag BBa_M1843_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtactagagccatagcacctatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcagctgtctcttatacacatcttactagagccatagcacctatactagagacggagaagcagcgaatactagatggccaacaccgtgtcggccaagaagatgacccgcaagatcgccaagcgtacggcgatcaaccgctcgcgccgttcgcggatgcgtaccttcgtccgcaaggtcgaggaggcgattgcgtccggcgatcagggccaggccctcaccgccctccgcgccgccgagccggagatcatgcgcgctgcccagaacggcatcgttcacaagaacaatgcctcgcggaaggtttcccgcctggccgcccgcgtgaaggccatcgcggcctgatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggcaaccctcagacactgccggtctttccagacctcgacatcttttcgcccgaatatgcctgcaaccgcgaaaaatacgcagcccgcgcccttagggactatccgctgcacttctataagccgctgaatttgtggatcgtcagcaagcacaaggatgtgcgctcggccttgttcactccgcaggtcttttcttctgtcgcgtttggcctgctccccccgcccgatgatatcgcgccgcgcgtgccggacctttacaccgacgtgcatctgccctcgatggacccgccggagcacaccaagctcagggttcccgtccagcaagccctcctccccggtcggctggtgggtaaggacgaggtcgttcgccggatcgccaacgaacttatcgataccttcatcgacaagggcgaatgcgatctcctccacgatttcagctacaaacttgcgctctacttgatcgtcgatctgctcgggatccccaaggaacgggcggaagattatcatcgctggtccaactgcttcttccagctgttcacccctaaggtaccggagcgcgccgacgcaagattcttcgtgccgatgccggaagaggtactgcggcagaactgggaaggcctggcggaggcgaacgactacctgcgcgaagtcgtcgagaaccttgatcgcaatcccggcaacaacatgctgtcaaacctgttgcagctgcgcgaaccggacggctcacgcacgatcacgatcagcgccaatgtttgcaacgcactggaatttggggcggctggtcatgacaccacggcaacgctgatcgcgcacctcacctatttcgtgctgaccacgccggacctcaaggacactcttaccgaggatccgtcgctgatccccgcggcgatttccgaaacgctgcgccgtcgctgtccggtcgacgggctgttccggcgaacgctgtcggatgtagaactatgcggccagaaaatcgaatccggatcgatcgtctacctcgatctcaccgctgcaaatcttgaccccgatgtattccccgaacctgagacgtttcgcctgaaccgcgacaatatcaaggaaatggtctctttcggcttcggtcgtcacgtctgcgcaggacagtacctgtcccgtatcgaagcaaaagcggcctacgaagaactgatgcgccgtattcccaacatgcgtctcgctgatggtttcaagctggaatacatgccttccgtggcgacgacggttctcaaggggcttccgctggtctgggacaaaaacaccggttaatactagatgatcatgagtaaagaatctattatttttgtcggtctgtttacagtgattttgagcgcggttatccttatgctgtcatatacaagcagcggccaagagcttaatcagtatgttaaaatagaagtccagcaaggcgacacactctggtcaattgctgatcaggtagccgatacaaaaaagataaacaaaaatgattttattgaatgggtagctgataaaaatcaacttcaaacctctgatatccagccgggtgatgagttagtgatcccattgaaaaagaagcatcaggatgcatatgaattagcaactgtaagataataatactagagtcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K123002_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac BBa_M1987_sequence 1 acggagaagcagcgaatactagatggccaacaccgtgtcggccaagaagatgacccgcaagatcgccaagcgtacggcgatcaaccgctcgcgccgttcgcggatgcgtaccttcgtccgcaaggtcgaggaggcgattgcgtccggcgatcagggccaggccctcaccgccctccgcgccgccgagccggagatcatgcgcgctgcccagaacggcatcgttcacaagaacaatgcctcgcggaaggtttcccgcctggccgcccgcgtgaaggccatcgcggcctgatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggcaaccctcagacactgccggtctttccagacctcgacatcttttcgcccgaatatgcctgcaaccgcgaaaaatacgcagcccgcgcccttagggactatccgctgcacttctataagccgctgaatttgtggatcgtcagcaagcacaaggatgtgcgctcggccttgttcactccgcaggtcttttcttctgtcgcgtttggcctgctccccccgcccgatgatatcgcgccgcgcgtgccggacctttacaccgacgtgcatctgccctcgatggacccgccggagcacaccaagctcagggttcccgtccagcaagccctcctccccggtcggctggtgggtaaggacgaggtcgttcgccggatcgccaacgaacttatcgataccttcatcgacaagggcgaatgcgatctcctccacgatttcagctacaaacttgcgctctacttgatcgtcgatctgctcgggatccccaaggaacgggcggaagattatcatcgctggtccaactgcttcttccagctgttcacccctaaggtaccggagcgcgccgacgcaagattcttcgtgccgatgccggaagaggtactgcggcagaactgggaaggcctggcggaggcgaacgactacctgcgcgaagtcgtcgagaaccttgatcgcaatcccggcaacaacatgctgtcaaacctgttgcagctgcgcgaaccggacggctcacgcacgatcacgatcagcgccaatgtttgcaacgcactggaatttggggcggctggtcatgacaccacggcaacgctgatcgcgcacctcacctatttcgtgctgaccacgccggacctcaaggacactcttaccgaggatccgtcgctgatccccgcggcgatttccgaaacgctgcgccgtcgctgtccggtcgacgggctgttccggcgaacgctgtcggatgtagaactatgcggccagaaaatcgaatccggatcgatcgtctacctcgatctcaccgctgcaaatcttgaccccgatgtattccccgaacctgagacgtttcgcctgaaccgcgacaatatcaaggaaatggtctctttcggcttcggtcgtcacgtctgcgcaggacagtacctgtcccgtatcgaagcaaaagcggcctacgaagaactgatgcgccgtattcccaacatgcgtctcgctgatggtttcaagctggaatacatgccttccgtggcgacgacggttctcaaggggcttccgctggtctgggacaaaaacaccggttaatactagatgatcatgagtaaagaatctattatttttgtcggtctgtttacagtgattttgagcgcggttatccttatgctgtcatatacaagcagcggccaagagcttaatcagtatgttaaaatagaagtccagcaaggcgacacactctggtcaattgctgatcaggtagccgatacaaaaaagataaacaaaaatgattttattgaatgggtagctgataaaaatcaacttcaaacctctgatatccagccgggtgatgagttagtgatcccattgaaaaagaagcatcaggatgcatatgaattagcaactgtaagataataatactagagtcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact BBa_M1887_sequence 1 ccatagcacctatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcagctgtctcttatacacatct BBa_M1845_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_M11107_sequence 1 atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctga BBa_K123009_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggcaaccctcagacactgccggtctttccagacctcgacatcttttcgcccgaatatgcctgcaaccgcgaaaaatacgcagcccgcgcccttagggactatccgctgcacttctataagccgctgaatttgtggatcgtcagcaagcacaaggatgtgcgctcggccttgttcactccgcaggtcttttcttctgtcgcgtttggcctgctccccccgcccgatgatatcgcgccgcgcgtgccggacctttacaccgacgtgcatctgccctcgatggacccgccggagcacaccaagctcagggttcccgtccagcaagccctcctccccggtcggctggtgggtaaggacgaggtcgttcgccggatcgccaacgaacttatcgataccttcatcgacaagggcgaatgcgatctcctccacgatttcagctacaaacttgcgctctacttgatcgtcgatctgctcgggatccccaaggaacgggcggaagattatcatcgctggtccaactgcttcttccagctgttcacccctaaggtaccggagcgcgccgacgcaagattcttcgtgccgatgccggaagaggtactgcggcagaactgggaaggcctggcggaggcgaacgactacctgcgcgaagtcgtcgagaaccttgatcgcaatcccggcaacaacatgctgtcaaacctgttgcagctgcgcgaaccggacggctcacgcacgatcacgatcagcgccaatgtttgcaacgcactggaatttggggcggctggtcatgacaccacggcaacgctgatcgcgcacctcacctatttcgtgctgaccacgccggacctcaaggacactcttaccgaggatccgtcgctgatccccgcggcgatttccgaaacgctgcgccgtcgctgtccggtcgacgggctgttccggcgaacgctgtcggatgtagaactatgcggccagaaaatcgaatccggatcgatcgtctacctcgatctcaccgctgcaaatcttgaccccgatgtattccccgaacctgagacgtttcgcctgaaccgcgacaatatcaaggaaatggtctctttcggcttcggtcgtcacgtctgcgcaggacagtacctgtcccgtatcgaagcaaaagcggcctacgaagaactgatgcgccgtattcccaacatgcgtctcgctgatggtttcaagctggaatacatgccttccgtggcgacgacggttctcaaggggcttccgctggtctgggacaaaaacaccggttaa BBa_B0034_sequence 1 aaagaggagaaa BBa_M1986_sequence 1 atggccaacaccgtgtcggccaagaagatgacccgcaagatcgccaagcgtacggcgatcaaccgctcgcgccgttcgcggatgcgtaccttcgtccgcaaggtcgaggaggcgattgcgtccggcgatcagggccaggccctcaccgccctccgcgccgccgagccggagatcatgcgcgctgcccagaacggcatcgttcacaagaacaatgcctcgcggaaggtttcccgcctggccgcccgcgtgaaggccatcgcggcctga BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_K302001_sequence 1 atgatcatgagtaaagaatctattatttttgtcggtctgtttacagtgattttgagcgcggttatccttatgctgtcatatacaagcagcggccaagagcttaatcagtatgttaaaatagaagtccagcaaggcgacacactctggtcaattgctgatcaggtagccgatacaaaaaagataaacaaaaatgattttattgaatgggtagctgataaaaatcaacttcaaacctctgatatccagccgggtgatgagttagtgatcccattgaaaaagaagcatcaggatgcatatgaattagcaactgtaagataataa BBa_K123008_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggcaaccctcagacactgccggtctttccagacctcgacatcttttcgcccgaatatgcctgcaaccgcgaaaaatacgcagcccgcgcccttagggactatccgctgcacttctataagccgctgaatttgtggatcgtcagcaagcacaaggatgtgcgctcggccttgttcactccgcaggtcttttcttctgtcgcgtttggcctgctccccccgcccgatgatatcgcgccgcgcgtgccggacctttacaccgacgtgcatctgccctcgatggacccgccggagcacaccaagctcagggttcccgtccagcaagccctcctccccggtcggctggtgggtaaggacgaggtcgttcgccggatcgccaacgaacttatcgataccttcatcgacaagggcgaatgcgatctcctccacgatttcagctacaaacttgcgctctacttgatcgtcgatctgctcgggatccccaaggaacgggcggaagattatcatcgctggtccaactgcttcttccagctgttcacccctaaggtaccggagcgcgccgacgcaagattcttcgtgccgatgccggaagaggtactgcggcagaactgggaaggcctggcggaggcgaacgactacctgcgcgaagtcgtcgagaaccttgatcgcaatcccggcaacaacatgctgtcaaacctgttgcagctgcgcgaaccggacggctcacgcacgatcacgatcagcgccaatgtttgcaacgcactggaatttggggcggctggtcatgacaccacggcaacgctgatcgcgcacctcacctatttcgtgctgaccacgccggacctcaaggacactcttaccgaggatccgtcgctgatccccgcggcgatttccgaaacgctgcgccgtcgctgtccggtcgacgggctgttccggcgaacgctgtcggatgtagaactatgcggccagaaaatcgaatccggatcgatcgtctacctcgatctcaccgctgcaaatcttgaccccgatgtattccccgaacctgagacgtttcgcctgaaccgcgacaatatcaaggaaatggtctctttcggcttcggtcgtcacgtctgcgcaggacagtacctgtcccgtatcgaagcaaaagcggcctacgaagaactgatgcgccgtattcccaacatgcgtctcgctgatggtttcaagctggaatacatgccttccgtggcgacgacggttctcaaggggcttccgctggtctgggacaaaaacaccggttaa BBa_M1844_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggccaacaccgtgtcggccaagaagatgacccgcaagatcgccaagcgtacggcgatcaaccgctcgcgccgttcgcggatgcgtaccttcgtccgcaaggtcgaggaggcgattgcgtccggcgatcagggccaggccctcaccgccctccgcgccgccgagccggagatcatgcgcgctgcccagaacggcatcgttcacaagaacaatgcctcgcggaaggtttcccgcctggccgcccgcgtgaaggccatcgcggcctgatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtactagagccatagcacctatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcagctgtctcttatacacatcttactagagccatagcacctatactagagacggagaagcagcgaatactagatggccaacaccgtgtcggccaagaagatgacccgcaagatcgccaagcgtacggcgatcaaccgctcgcgccgttcgcggatgcgtaccttcgtccgcaaggtcgaggaggcgattgcgtccggcgatcagggccaggccctcaccgccctccgcgccgccgagccggagatcatgcgcgctgcccagaacggcatcgttcacaagaacaatgcctcgcggaaggtttcccgcctggccgcccgcgtgaaggccatcgcggcctgatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggcaaccctcagacactgccggtctttccagacctcgacatcttttcgcccgaatatgcctgcaaccgcgaaaaatacgcagcccgcgcccttagggactatccgctgcacttctataagccgctgaatttgtggatcgtcagcaagcacaaggatgtgcgctcggccttgttcactccgcaggtcttttcttctgtcgcgtttggcctgctccccccgcccgatgatatcgcgccgcgcgtgccggacctttacaccgacgtgcatctgccctcgatggacccgccggagcacaccaagctcagggttcccgtccagcaagccctcctccccggtcggctggtgggtaaggacgaggtcgttcgccggatcgccaacgaacttatcgataccttcatcgacaagggcgaatgcgatctcctccacgatttcagctacaaacttgcgctctacttgatcgtcgatctgctcgggatccccaaggaacgggcggaagattatcatcgctggtccaactgcttcttccagctgttcacccctaaggtaccggagcgcgccgacgcaagattcttcgtgccgatgccggaagaggtactgcggcagaactgggaaggcctggcggaggcgaacgactacctgcgcgaagtcgtcgagaaccttgatcgcaatcccggcaacaacatgctgtcaaacctgttgcagctgcgcgaaccggacggctcacgcacgatcacgatcagcgccaatgtttgcaacgcactggaatttggggcggctggtcatgacaccacggcaacgctgatcgcgcacctcacctatttcgtgctgaccacgccggacctcaaggacactcttaccgaggatccgtcgctgatccccgcggcgatttccgaaacgctgcgccgtcgctgtccggtcgacgggctgttccggcgaacgctgtcggatgtagaactatgcggccagaaaatcgaatccggatcgatcgtctacctcgatctcaccgctgcaaatcttgaccccgatgtattccccgaacctgagacgtttcgcctgaaccgcgacaatatcaaggaaatggtctctttcggcttcggtcgtcacgtctgcgcaggacagtacctgtcccgtatcgaagcaaaagcggcctacgaagaactgatgcgccgtattcccaacatgcgtctcgctgatggtttcaagctggaatacatgccttccgtggcgacgacggttctcaaggggcttccgctggtctgggacaaaaacaccggttaatactagatgatcatgagtaaagaatctattatttttgtcggtctgtttacagtgattttgagcgcggttatccttatgctgtcatatacaagcagcggccaagagcttaatcagtatgttaaaatagaagtccagcaaggcgacacactctggtcaattgctgatcaggtagccgatacaaaaaagataaacaaaaatgattttattgaatgggtagctgataaaaatcaacttcaaacctctgatatccagccgggtgatgagttagtgatcccattgaaaaagaagcatcaggatgcatatgaattagcaactgtaagataataatactagagtcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_M1888_sequence 1 ccatagcaccta BBa_M1877_sequence 1 ccatagcaccta BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K123001_sequence 1 atggccggcaaccctcagacactgccggtctttccagacctcgacatcttttcgcccgaatatgcctgcaaccgcgaaaaatacgcagcccgcgcccttagggactatccgctgcacttctataagccgctgaatttgtggatcgtcagcaagcacaaggatgtgcgctcggccttgttcactccgcaggtcttttcttctgtcgcgtttggcctgctccccccgcccgatgatatcgcgccgcgcgtgccggacctttacaccgacgtgcatctgccctcgatggacccgccggagcacaccaagctcagggttcccgtccagcaagccctcctccccggtcggctggtgggtaaggacgaggtcgttcgccggatcgccaacgaacttatcgataccttcatcgacaagggcgaatgcgatctcctccacgatttcagctacaaacttgcgctctacttgatcgtcgatctgctcgggatccccaaggaacgggcggaagattatcatcgctggtccaactgcttcttccagctgttcacccctaaggtaccggagcgcgccgacgcaagattcttcgtgccgatgccggaagaggtactgcggcagaactgggaaggcctggcggaggcgaacgactacctgcgcgaagtcgtcgagaaccttgatcgcaatcccggcaacaacatgctgtcaaacctgttgcagctgcgcgaaccggacggctcacgcacgatcacgatcagcgccaatgtttgcaacgcactggaatttggggcggctggtcatgacaccacggcaacgctgatcgcgcacctcacctatttcgtgctgaccacgccggacctcaaggacactcttaccgaggatccgtcgctgatccccgcggcgatttccgaaacgctgcgccgtcgctgtccggtcgacgggctgttccggcgaacgctgtcggatgtagaactatgcggccagaaaatcgaatccggatcgatcgtctacctcgatctcaccgctgcaaatcttgaccccgatgtattccccgaacctgagacgtttcgcctgaaccgcgacaatatcaaggaaatggtctctttcggcttcggtcgtcacgtctgcgcaggacagtacctgtcccgtatcgaagcaaaagcggcctacgaagaactgatgcgccgtattcccaacatgcgtctcgctgatggtttcaagctggaatacatgccttccgtggcgacgacggttctcaaggggcttccgctggtctgggacaaaaacaccggttaa BBa_I742145_sequence 1 acggagaagcagcgaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z