BBa_M1844
type: ComponentDefinition
persistent identity: BBa M1844
display id: BBa_M1844
version: 1
was generated by: iGEM to SBOL conversion
description: RPS Tgene from Methilobacteriun chloromeneticum
created: 2012-03-27T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: E. coli
owned by: james
owned by: myers
mutable description: Composite part that induces post translational inhibition of arginine and ornithine decarboxylase
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12257
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: undefined
star: false
creator: sarah guzman
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K123002
component: BBa_M1986
component: BBa_B0034
component: BBa_B0010
component: BBa_M1843
component: BBa_B0012
sequence annotation: BBa_K123002
sequence annotation: BBa_B0034
sequence annotation: BBa_M1986
sequence annotation: BBa_M1843
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_M1844_sequence/1

BBa_K123002
type: Component
persistent identity: component2171567
display id: component2171567
version: 1
top level: BBa_M1844
definition: BBa_K123002
access: public

BBa_M1986
type: Component
persistent identity: component2171573
display id: component2171573
version: 1
top level: BBa_M1844
definition: BBa_M1986
access: public

BBa_B0034
type: Component
persistent identity: component2171569
display id: component2171569
version: 1
top level: BBa_M1844
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component2171656
display id: component2171656
version: 1
top level: BBa_M1844
definition: BBa_B0010
access: public

BBa_M1843
type: Component
persistent identity: component2171655
display id: component2171655
version: 1
top level: BBa_M1844
definition: BBa_M1843
access: public

BBa_B0012
type: Component
persistent identity: component2171658
display id: component2171658
version: 1
top level: BBa_M1844
definition: BBa_B0012
access: public

BBa_K123002
type: SequenceAnnotation
persistent identity: annotation2171567
display id: annotation2171567
version: 1
top level: BBa_M1844
location: https://synbiohub.org/public/igem/BBa_M1844/annotation2171567/range2171567/1
component: BBa_K123002

https://synbiohub.org/public/igem/BBa_M1844/annotation2171567/range2171567/1
type: Range
persistent identity: range2171567
display id: range2171567
version: 1
top level: BBa_M1844
direction: direction/reverse
start: 1
end: 742
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2171569
display id: annotation2171569
version: 1
top level: BBa_M1844
location: https://synbiohub.org/public/igem/BBa_M1844/annotation2171569/range2171569/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M1844/annotation2171569/range2171569/1
type: Range
persistent identity: range2171569
display id: range2171569
version: 1
top level: BBa_M1844
direction: direction/reverse
start: 751
end: 762
orientation: inline

BBa_M1986
type: SequenceAnnotation
persistent identity: annotation2171573
display id: annotation2171573
version: 1
top level: BBa_M1844
location: https://synbiohub.org/public/igem/BBa_M1844/annotation2171573/range2171573/1
component: BBa_M1986

https://synbiohub.org/public/igem/BBa_M1844/annotation2171573/range2171573/1
type: Range
persistent identity: range2171573
display id: range2171573
version: 1
top level: BBa_M1844
direction: direction/reverse
start: 769
end: 1035
orientation: inline

BBa_M1843
type: SequenceAnnotation
persistent identity: annotation2171655
display id: annotation2171655
version: 1
top level: BBa_M1844
location: https://synbiohub.org/public/igem/BBa_M1844/annotation2171655/range2171655/1
component: BBa_M1843

https://synbiohub.org/public/igem/BBa_M1844/annotation2171655/range2171655/1
type: Range
persistent identity: range2171655
display id: range2171655
version: 1
top level: BBa_M1844
direction: direction/reverse
start: 1042
end: 6753
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2171656
display id: annotation2171656
version: 1
top level: BBa_M1844
location: https://synbiohub.org/public/igem/BBa_M1844/annotation2171656/range2171656/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M1844/annotation2171656/range2171656/1
type: Range
persistent identity: range2171656
display id: range2171656
version: 1
top level: BBa_M1844
direction: direction/forward
start: 6762
end: 6841
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2171658
display id: annotation2171658
version: 1
top level: BBa_M1844
location: https://synbiohub.org/public/igem/BBa_M1844/annotation2171658/range2171658/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M1844/annotation2171658/range2171658/1
type: Range
persistent identity: range2171658
display id: range2171658
version: 1
top level: BBa_M1844
direction: direction/forward
start: 6850
end: 6890
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_M1986
type: ComponentDefinition
persistent identity: BBa M1986
display id: BBa_M1986
version: 1
was generated by: iGEM to SBOL conversion
description: rpsT gene from Methylobacterium Chloromethanicum
created: 2012-03-26T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: Methylobacterium Chloromethanicum. The sequence comes from the site http://www.ncbi.nlm.nih.gov/nuccore/218528082?from=359&to=625
owned by: james
owned by: myers
mutable description: The rpsT gene binds directly to the 16s rRNA and induces post translational inhibition of arginine and ornithine decarboxylase
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 12223
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: I copied and pasted the sequence from NCBI.
star: false
creator: David Harris
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: Open reading frame
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M1986_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2171187
display id: annotation2171187
version: 1
top level: BBa_M1986
location: https://synbiohub.org/public/igem/BBa_M1986/annotation2171187/range2171187/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M1986/annotation2171187/range2171187/1
type: Range
persistent identity: range2171187
display id: range2171187
version: 1
top level: BBa_M1986
direction: direction/forward
start: 1
end: 3
orientation: inline

Open reading frame
type: SequenceAnnotation
persistent identity: annotation2171189
display id: annotation2171189
version: 1
top level: BBa_M1986
location: https://synbiohub.org/public/igem/BBa_M1986/annotation2171189/range2171189/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_M1986/annotation2171189/range2171189/1
type: Range
persistent identity: range2171189
display id: range2171189
version: 1
top level: BBa_M1986
direction: direction/forward
start: 4
end: 264
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2171188
display id: annotation2171188
version: 1
top level: BBa_M1986
location: https://synbiohub.org/public/igem/BBa_M1986/annotation2171188/range2171188/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1986/annotation2171188/range2171188/1
type: Range
persistent identity: range2171188
display id: range2171188
version: 1
top level: BBa_M1986
direction: direction/forward
start: 265
end: 267
orientation: inline

BBa_M1843
type: ComponentDefinition
persistent identity: BBa M1843
display id: BBa_M1843
version: 1
was generated by: iGEM to SBOL conversion
description: His
created: 2012-03-26T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: His
owned by: james
owned by: myers
mutable description: His
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12257
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: His
star: false
creator: sarah guzman
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_M1845
component: BBa_M1872
component: BBa_M1987
component: BBa_M1877
component: BBa_M1887
component: BBa_E1010
component: BBa_M1888
sequence annotation: BBa_M1845
sequence annotation: BBa_M1872
sequence annotation: BBa_M1887
sequence annotation: BBa_M1888
sequence annotation: BBa_M1877
sequence annotation: BBa_M1987
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_M1843_sequence/1

BBa_M1845
type: Component
persistent identity: component2171287
display id: component2171287
version: 1
top level: BBa_M1843
definition: BBa_M1845
access: public

BBa_M1872
type: Component
persistent identity: component2171285
display id: component2171285
version: 1
top level: BBa_M1843
definition: BBa_M1872
access: public

BBa_M1987
type: Component
persistent identity: component2171363
display id: component2171363
version: 1
top level: BBa_M1843
definition: BBa_M1987
access: public

BBa_M1877
type: Component
persistent identity: component2171316
display id: component2171316
version: 1
top level: BBa_M1843
definition: BBa_M1877
access: public

BBa_M1887
type: Component
persistent identity: component2171313
display id: component2171313
version: 1
top level: BBa_M1843
definition: BBa_M1887
access: public

BBa_E1010
type: Component
persistent identity: component2171341
display id: component2171341
version: 1
top level: BBa_M1843
definition: mRFP1
access: public

BBa_M1888
type: Component
persistent identity: component2171317
display id: component2171317
version: 1
top level: BBa_M1843
definition: BBa_M1888
access: public

BBa_M1845
type: SequenceAnnotation
persistent identity: annotation2171287
display id: annotation2171287
version: 1
top level: BBa_M1843
location: https://synbiohub.org/public/igem/BBa_M1843/annotation2171287/range2171287/1
component: BBa_M1845

https://synbiohub.org/public/igem/BBa_M1843/annotation2171287/range2171287/1
type: Range
persistent identity: range2171287
display id: range2171287
version: 1
top level: BBa_M1843
direction: direction/reverse
start: 1
end: 717
orientation: inline

BBa_M1872
type: SequenceAnnotation
persistent identity: annotation2171285
display id: annotation2171285
version: 1
top level: BBa_M1843
location: https://synbiohub.org/public/igem/BBa_M1843/annotation2171285/range2171285/1
component: BBa_M1872

https://synbiohub.org/public/igem/BBa_M1843/annotation2171285/range2171285/1
type: Range
persistent identity: range2171285
display id: range2171285
version: 1
top level: BBa_M1843
direction: direction/reverse
start: 1
end: 717
orientation: inline

BBa_M1887
type: SequenceAnnotation
persistent identity: annotation2171313
display id: annotation2171313
version: 1
top level: BBa_M1843
location: https://synbiohub.org/public/igem/BBa_M1843/annotation2171313/range2171313/1
component: BBa_M1887

https://synbiohub.org/public/igem/BBa_M1843/annotation2171313/range2171313/1
type: Range
persistent identity: range2171313
display id: range2171313
version: 1
top level: BBa_M1843
direction: direction/reverse
start: 726
end: 1678
orientation: inline

BBa_M1888
type: SequenceAnnotation
persistent identity: annotation2171317
display id: annotation2171317
version: 1
top level: BBa_M1843
location: https://synbiohub.org/public/igem/BBa_M1843/annotation2171317/range2171317/1
component: BBa_M1888

https://synbiohub.org/public/igem/BBa_M1843/annotation2171317/range2171317/1
type: Range
persistent identity: range2171317
display id: range2171317
version: 1
top level: BBa_M1843
direction: direction/reverse
start: 1687
end: 1698
orientation: inline

BBa_M1877
type: SequenceAnnotation
persistent identity: annotation2171316
display id: annotation2171316
version: 1
top level: BBa_M1843
location: https://synbiohub.org/public/igem/BBa_M1843/annotation2171316/range2171316/1
component: BBa_M1877

https://synbiohub.org/public/igem/BBa_M1843/annotation2171316/range2171316/1
type: Range
persistent identity: range2171316
display id: range2171316
version: 1
top level: BBa_M1843
direction: direction/reverse
start: 1687
end: 1698
orientation: inline

BBa_M1987
type: SequenceAnnotation
persistent identity: annotation2171363
display id: annotation2171363
version: 1
top level: BBa_M1843
location: https://synbiohub.org/public/igem/BBa_M1843/annotation2171363/range2171363/1
component: BBa_M1987

https://synbiohub.org/public/igem/BBa_M1843/annotation2171363/range2171363/1
type: Range
persistent identity: range2171363
display id: range2171363
version: 1
top level: BBa_M1843
direction: direction/reverse
start: 1707
end: 5712
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2171341
display id: annotation2171341
version: 1
top level: BBa_M1843
location: https://synbiohub.org/public/igem/BBa_M1843/annotation2171341/range2171341/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_M1843/annotation2171341/range2171341/1
type: Range
persistent identity: range2171341
display id: range2171341
version: 1
top level: BBa_M1843
direction: direction/reverse
start: 2814
end: 3494
orientation: inline

BBa_K123002
type: ComponentDefinition
persistent identity: BBa K123002
display id: BBa_K123002
version: 1
was generated by: iGEM to SBOL conversion
description: LacIQ ERE TetR
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: The Estrogen Responsive Element was taken from the Human Estrogen Responsive Element
owned by: james
owned by: myers
mutable description: This composite part contains LacIQ (BBa_I14032) and Estrogen Receptor Element (ERE) and TetR (BBa_C0040). The Estrogen Responsive element is to be used in conjunction with ER (Estrogen Receptor). This design involves ER binding to the ERE and disrupting the constituative promoter LacIQ from producing TetR via physically getting in the way of the polymerase.
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: There is some data that suggests the ER is toxic to the cell. However in our experiments while we did see a decrease in growth rate we did not find ER to be fatal to the cell.
star: true
creator: Jason Gardiner
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_I14032
sequence annotation: P(Lac) IQ
sequence annotation: -35
sequence annotation: -10
sequence annotation: ERE
sequence annotation: BBa_C0040
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: barcode
sequence: https://synbiohub.org/public/igem/BBa_K123002_sequence/1

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation1992720
display id: annotation1992720
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992720/range1992720/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K123002/annotation1992720/range1992720/1
type: Range
persistent identity: range1992720
display id: range1992720
version: 1
top level: BBa_K123002
direction: direction/reverse
start: 1
end: 37
orientation: inline

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1992717
display id: annotation1992717
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992717/range1992717/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K123002/annotation1992717/range1992717/1
type: Range
persistent identity: range1992717
display id: range1992717
version: 1
top level: BBa_K123002
direction: direction/forward
start: 1
end: 37
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1992718
display id: annotation1992718
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992718/range1992718/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K123002/annotation1992718/range1992718/1
type: Range
persistent identity: range1992718
display id: range1992718
version: 1
top level: BBa_K123002
direction: direction/forward
start: 3
end: 8
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1992719
display id: annotation1992719
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992719/range1992719/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K123002/annotation1992719/range1992719/1
type: Range
persistent identity: range1992719
display id: range1992719
version: 1
top level: BBa_K123002
direction: direction/forward
start: 26
end: 31
orientation: inline

ERE
type: SequenceAnnotation
persistent identity: annotation1992733
display id: annotation1992733
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992733/range1992733/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K123002/annotation1992733/range1992733/1
type: Range
persistent identity: range1992733
display id: range1992733
version: 1
top level: BBa_K123002
direction: direction/forward
start: 43
end: 57
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation1992723
display id: annotation1992723
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992723/range1992723/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K123002/annotation1992723/range1992723/1
type: Range
persistent identity: range1992723
display id: range1992723
version: 1
top level: BBa_K123002
direction: direction/reverse
start: 44
end: 703
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation1992721
display id: annotation1992721
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992721/range1992721/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K123002/annotation1992721/range1992721/1
type: Range
persistent identity: range1992721
display id: range1992721
version: 1
top level: BBa_K123002
direction: direction/forward
start: 47
end: 663
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation1992722
display id: annotation1992722
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992722/range1992722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K123002/annotation1992722/range1992722/1
type: Range
persistent identity: range1992722
display id: range1992722
version: 1
top level: BBa_K123002
direction: direction/forward
start: 664
end: 697
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1992724
display id: annotation1992724
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992724/range1992724/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K123002/annotation1992724/range1992724/1
type: Range
persistent identity: range1992724
display id: range1992724
version: 1
top level: BBa_K123002
direction: direction/reverse
start: 704
end: 728
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_M1987
type: ComponentDefinition
persistent identity: BBa M1987
display id: BBa_M1987
version: 1
was generated by: iGEM to SBOL conversion
description: Composite with rpsT gene
created: 2012-03-26T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: the source of the gene is from Methylobacterium chloromethanicum. Other parts are from the registry.
owned by: james
owned by: myers
mutable description: the rpsT gene with a promoter-RBS- and two TC stops added
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 12223
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: I just found different ribosome binding sites and Tc stops from the registry. The promoter is for the glucose controlled ompC porin gene. The RBS is the native rbs for the sam5 gene of Saccharothrix espanaensis The first stop stops RNA polymerase from transcribing the TetR gene. The second codes for a gene that inhibits cell division
star: false
creator: David Harris
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_M1986
component: BBa_I742145
component: BBa_K302001
component: BBa_K123009
component: BBa_R0082
sequence annotation: BBa_I742145
sequence annotation: BBa_M1986
sequence annotation: BBa_K123009
sequence annotation: BBa_K302001
sequence annotation: BBa_R0082
sequence: https://synbiohub.org/public/igem/BBa_M1987_sequence/1

BBa_M1986
type: Component
persistent identity: component2295738
display id: component2295738
version: 1
top level: BBa_M1987
definition: BBa_M1986
access: public

BBa_I742145
type: Component
persistent identity: component2295734
display id: component2295734
version: 1
top level: BBa_M1987
definition: BBa_I742145
access: public

BBa_K302001
type: Component
persistent identity: component2295772
display id: component2295772
version: 1
top level: BBa_M1987
definition: BBa_K302001
access: public

BBa_K123009
type: Component
persistent identity: component2295770
display id: component2295770
version: 1
top level: BBa_M1987
definition: BBa_K123009
access: public

BBa_R0082
type: Component
persistent identity: component2295778
display id: component2295778
version: 1
top level: BBa_M1987
definition: OmpR
access: public

BBa_I742145
type: SequenceAnnotation
persistent identity: annotation2295734
display id: annotation2295734
version: 1
top level: BBa_M1987
location: https://synbiohub.org/public/igem/BBa_M1987/annotation2295734/range2295734/1
component: BBa_I742145

https://synbiohub.org/public/igem/BBa_M1987/annotation2295734/range2295734/1
type: Range
persistent identity: range2295734
display id: range2295734
version: 1
top level: BBa_M1987
direction: direction/reverse
start: 1
end: 16
orientation: inline

BBa_M1986
type: SequenceAnnotation
persistent identity: annotation2295738
display id: annotation2295738
version: 1
top level: BBa_M1987
location: https://synbiohub.org/public/igem/BBa_M1987/annotation2295738/range2295738/1
component: BBa_M1986

https://synbiohub.org/public/igem/BBa_M1987/annotation2295738/range2295738/1
type: Range
persistent identity: range2295738
display id: range2295738
version: 1
top level: BBa_M1987
direction: direction/reverse
start: 23
end: 289
orientation: inline

BBa_K123009
type: SequenceAnnotation
persistent identity: annotation2295770
display id: annotation2295770
version: 1
top level: BBa_M1987
location: https://synbiohub.org/public/igem/BBa_M1987/annotation2295770/range2295770/1
component: BBa_K123009

https://synbiohub.org/public/igem/BBa_M1987/annotation2295770/range2295770/1
type: Range
persistent identity: range2295770
display id: range2295770
version: 1
top level: BBa_M1987
direction: direction/reverse
start: 298
end: 3563
orientation: inline

BBa_K302001
type: SequenceAnnotation
persistent identity: annotation2295772
display id: annotation2295772
version: 1
top level: BBa_M1987
location: https://synbiohub.org/public/igem/BBa_M1987/annotation2295772/range2295772/1
component: BBa_K302001

https://synbiohub.org/public/igem/BBa_M1987/annotation2295772/range2295772/1
type: Range
persistent identity: range2295772
display id: range2295772
version: 1
top level: BBa_M1987
direction: direction/reverse
start: 3570
end: 3890
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2295778
display id: annotation2295778
version: 1
top level: BBa_M1987
location: https://synbiohub.org/public/igem/BBa_M1987/annotation2295778/range2295778/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_M1987/annotation2295778/range2295778/1
type: Range
persistent identity: range2295778
display id: range2295778
version: 1
top level: BBa_M1987
direction: direction/reverse
start: 3899
end: 4006
orientation: inline

BBa_M1872
type: ComponentDefinition
persistent identity: BBa M1872
display id: BBa_M1872
version: 1
was generated by: iGEM to SBOL conversion
description: GFP
created: 2012-03-26T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: Ampicilin
owned by: james
owned by: myers
mutable description: Ampicilin
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12257
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: Ampicillin: atgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaac gctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaaga tccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcc cgtgttgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcac agaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggcca acttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgcctt gatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgcagcaatggcaacaac gttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaag ttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtct cgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaac tatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaa
star: false
creator: sarah guzman
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence annotation: start
sequence annotation: stop
sequence annotation: operator
sequence: https://synbiohub.org/public/igem/BBa_M1872_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2171235
display id: annotation2171235
version: 1
top level: BBa_M1872
location: https://synbiohub.org/public/igem/BBa_M1872/annotation2171235/range2171235/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1872/annotation2171235/range2171235/1
type: Range
persistent identity: range2171235
display id: range2171235
version: 1
top level: BBa_M1872
direction: direction/forward
start: 1
end: 299
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2171237
display id: annotation2171237
version: 1
top level: BBa_M1872
location: https://synbiohub.org/public/igem/BBa_M1872/annotation2171237/range2171237/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1872/annotation2171237/range2171237/1
type: Range
persistent identity: range2171237
display id: range2171237
version: 1
top level: BBa_M1872
direction: direction/forward
start: 1
end: 200
orientation: inline

operator
type: SequenceAnnotation
persistent identity: annotation2171236
display id: annotation2171236
version: 1
top level: BBa_M1872
location: https://synbiohub.org/public/igem/BBa_M1872/annotation2171236/range2171236/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_M1872/annotation2171236/range2171236/1
type: Range
persistent identity: range2171236
display id: range2171236
version: 1
top level: BBa_M1872
direction: direction/forward
start: 300
end: 500
orientation: inline

BBa_M1877
type: ComponentDefinition
persistent identity: BBa M1877
display id: BBa_M1877
version: 1
was generated by: iGEM to SBOL conversion
description: human insulin
created: 2012-03-26T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: dfadf
owned by: james
owned by: myers
mutable description: zedfd
discontinued: false
dominant: true
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12283
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: dfdf
star: false
creator: Elizabeth Martinez
type: DnaRegion
role: partType/Temporary
role: SO:0000110
sequence annotation: insulin start
sequence annotation: coding sequence
sequence annotation: insulin
sequence annotation: insulin stop
sequence: https://synbiohub.org/public/igem/BBa_M1877_sequence/1

insulin start
type: SequenceAnnotation
persistent identity: annotation2171242
display id: annotation2171242
version: 1
top level: BBa_M1877
location: https://synbiohub.org/public/igem/BBa_M1877/annotation2171242/range2171242/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1877/annotation2171242/range2171242/1
type: Range
persistent identity: range2171242
display id: range2171242
version: 1
top level: BBa_M1877
direction: direction/forward
start: 1
end: 1
orientation: inline

coding sequence
type: SequenceAnnotation
persistent identity: annotation2171241
display id: annotation2171241
version: 1
top level: BBa_M1877
location: https://synbiohub.org/public/igem/BBa_M1877/annotation2171241/range2171241/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_M1877/annotation2171241/range2171241/1
type: Range
persistent identity: range2171241
display id: range2171241
version: 1
top level: BBa_M1877
direction: direction/forward
start: 1
end: 12
orientation: inline

insulin
type: SequenceAnnotation
persistent identity: annotation2171243
display id: annotation2171243
version: 1
top level: BBa_M1877
location: https://synbiohub.org/public/igem/BBa_M1877/annotation2171243/range2171243/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_M1877/annotation2171243/range2171243/1
type: Range
persistent identity: range2171243
display id: range2171243
version: 1
top level: BBa_M1877
direction: direction/forward
start: 1
end: 12
orientation: inline

insulin stop
type: SequenceAnnotation
persistent identity: annotation2171248
display id: annotation2171248
version: 1
top level: BBa_M1877
location: https://synbiohub.org/public/igem/BBa_M1877/annotation2171248/range2171248/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1877/annotation2171248/range2171248/1
type: Range
persistent identity: range2171248
display id: range2171248
version: 1
top level: BBa_M1877
direction: direction/forward
start: 12
end: 12
orientation: inline

BBa_M1845
type: ComponentDefinition
persistent identity: BBa M1845
display id: BBa_M1845
version: 1
was generated by: iGEM to SBOL conversion
description: His6
created: 2012-03-26T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: Hus6
owned by: james
owned by: myers
mutable description: His6
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12257
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: catcatcaccatcaccac
star: false
creator: sarah guzman
type: DnaRegion
role: partType/Composite
role: SO:0000804
sequence annotation: BBa_M1872
sequence annotation: start
sequence annotation: stop
sequence annotation: operator
sequence: https://synbiohub.org/public/igem/BBa_M1845_sequence/1

BBa_M1872
type: SequenceAnnotation
persistent identity: annotation2171247
display id: annotation2171247
version: 1
top level: BBa_M1845
location: https://synbiohub.org/public/igem/BBa_M1845/annotation2171247/range2171247/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_M1845/annotation2171247/range2171247/1
type: Range
persistent identity: range2171247
display id: range2171247
version: 1
top level: BBa_M1845
direction: direction/reverse
start: 1
end: 717
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2171244
display id: annotation2171244
version: 1
top level: BBa_M1845
location: https://synbiohub.org/public/igem/BBa_M1845/annotation2171244/range2171244/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1845/annotation2171244/range2171244/1
type: Range
persistent identity: range2171244
display id: range2171244
version: 1
top level: BBa_M1845
direction: direction/forward
start: 1
end: 299
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2171245
display id: annotation2171245
version: 1
top level: BBa_M1845
location: https://synbiohub.org/public/igem/BBa_M1845/annotation2171245/range2171245/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_M1845/annotation2171245/range2171245/1
type: Range
persistent identity: range2171245
display id: range2171245
version: 1
top level: BBa_M1845
direction: direction/forward
start: 1
end: 200
orientation: inline

operator
type: SequenceAnnotation
persistent identity: annotation2171246
display id: annotation2171246
version: 1
top level: BBa_M1845
location: https://synbiohub.org/public/igem/BBa_M1845/annotation2171246/range2171246/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_M1845/annotation2171246/range2171246/1
type: Range
persistent identity: range2171246
display id: range2171246
version: 1
top level: BBa_M1845
direction: direction/forward
start: 300
end: 500
orientation: inline

BBa_M1887
type: ComponentDefinition
persistent identity: BBa M1887
display id: BBa_M1887
version: 1
was generated by: iGEM to SBOL conversion
description: fgf
created: 2012-03-26T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: fgf
owned by: james
owned by: myers
mutable description: gfgf
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12283
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: fgfg
star: false
creator: Elizabeth Martinez
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_M1877
component: BBa_M1000
component: BBa_M1999
sequence annotation: BBa_M1877
sequence annotation: BBa_M1999
sequence annotation: BBa_M1000
sequence: https://synbiohub.org/public/igem/BBa_M1887_sequence/1

BBa_M1877
type: Component
persistent identity: component2171261
display id: component2171261
version: 1
top level: BBa_M1887
definition: BBa_M1877
access: public

BBa_M1000
type: Component
persistent identity: component2171282
display id: component2171282
version: 1
top level: BBa_M1887
definition: BBa_M1000
access: public

BBa_M1999
type: Component
persistent identity: component2171279
display id: component2171279
version: 1
top level: BBa_M1887
definition: BBa_M1999
access: public

BBa_M1877
type: SequenceAnnotation
persistent identity: annotation2171261
display id: annotation2171261
version: 1
top level: BBa_M1887
location: https://synbiohub.org/public/igem/BBa_M1887/annotation2171261/range2171261/1
component: BBa_M1877

https://synbiohub.org/public/igem/BBa_M1887/annotation2171261/range2171261/1
type: Range
persistent identity: range2171261
display id: range2171261
version: 1
top level: BBa_M1887
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_M1999
type: SequenceAnnotation
persistent identity: annotation2171279
display id: annotation2171279
version: 1
top level: BBa_M1887
location: https://synbiohub.org/public/igem/BBa_M1887/annotation2171279/range2171279/1
component: BBa_M1999

https://synbiohub.org/public/igem/BBa_M1887/annotation2171279/range2171279/1
type: Range
persistent identity: range2171279
display id: range2171279
version: 1
top level: BBa_M1887
direction: direction/reverse
start: 21
end: 923
orientation: inline

BBa_M1000
type: SequenceAnnotation
persistent identity: annotation2171282
display id: annotation2171282
version: 1
top level: BBa_M1887
location: https://synbiohub.org/public/igem/BBa_M1887/annotation2171282/range2171282/1
component: BBa_M1000

https://synbiohub.org/public/igem/BBa_M1887/annotation2171282/range2171282/1
type: Range
persistent identity: range2171282
display id: range2171282
version: 1
top level: BBa_M1887
direction: direction/reverse
start: 932
end: 953
orientation: inline

BBa_M1888
type: ComponentDefinition
persistent identity: BBa M1888
display id: BBa_M1888
version: 1
was generated by: iGEM to SBOL conversion
description: fdf
created: 2012-03-26T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: dfdf
owned by: james
owned by: myers
mutable description: dfdf
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12283
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: dfdf
star: false
is replaced by: BBa_M1877
creator: Elizabeth Martinez
type: DnaRegion
role: partType/Composite
role: SO:0000804
sequence annotation: coding sequence
sequence annotation: insulin start
sequence annotation: insulin
sequence annotation: BBa_M1877
sequence annotation: insulin stop
sequence: https://synbiohub.org/public/igem/BBa_M1888_sequence/1

coding sequence
type: SequenceAnnotation
persistent identity: annotation2171251
display id: annotation2171251
version: 1
top level: BBa_M1888
location: https://synbiohub.org/public/igem/BBa_M1888/annotation2171251/range2171251/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1888/annotation2171251/range2171251/1
type: Range
persistent identity: range2171251
display id: range2171251
version: 1
top level: BBa_M1888
direction: direction/forward
start: 1
end: 12
orientation: inline

insulin start
type: SequenceAnnotation
persistent identity: annotation2171250
display id: annotation2171250
version: 1
top level: BBa_M1888
location: https://synbiohub.org/public/igem/BBa_M1888/annotation2171250/range2171250/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1888/annotation2171250/range2171250/1
type: Range
persistent identity: range2171250
display id: range2171250
version: 1
top level: BBa_M1888
direction: direction/forward
start: 1
end: 1
orientation: inline

insulin
type: SequenceAnnotation
persistent identity: annotation2171252
display id: annotation2171252
version: 1
top level: BBa_M1888
location: https://synbiohub.org/public/igem/BBa_M1888/annotation2171252/range2171252/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_M1888/annotation2171252/range2171252/1
type: Range
persistent identity: range2171252
display id: range2171252
version: 1
top level: BBa_M1888
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_M1877
type: SequenceAnnotation
persistent identity: annotation2171255
display id: annotation2171255
version: 1
top level: BBa_M1888
location: https://synbiohub.org/public/igem/BBa_M1888/annotation2171255/range2171255/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_M1888/annotation2171255/range2171255/1
type: Range
persistent identity: range2171255
display id: range2171255
version: 1
top level: BBa_M1888
direction: direction/reverse
start: 1
end: 12
orientation: inline

insulin stop
type: SequenceAnnotation
persistent identity: annotation2171254
display id: annotation2171254
version: 1
top level: BBa_M1888
location: https://synbiohub.org/public/igem/BBa_M1888/annotation2171254/range2171254/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_M1888/annotation2171254/range2171254/1
type: Range
persistent identity: range2171254
display id: range2171254
version: 1
top level: BBa_M1888
direction: direction/forward
start: 12
end: 12
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
top level: OmpR
direction: direction/forward
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
top level: OmpR
direction: direction/forward
start: 54
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
top level: OmpR
direction: direction/forward
start: 75
end: 80
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
top level: OmpR
direction: direction/forward
start: 98
end: 103
orientation: inline

BBa_I742145
type: ComponentDefinition
persistent identity: BBa I742145
display id: BBa_I742145
version: 1
was generated by: iGEM to SBOL conversion
description: sam5 native rbs
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Saccharothrix espanaensis DSM 44229
owned by: james
owned by: myers
mutable description: This is the native ribosome binding site for sam5 (4-coumarate-3-hydroxylase) of the bacterium Saccharothrix espanaensis.
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is a virtual part to be added to coding sequence I742141.
star: false
creator: Chris French
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_I742145_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1955475
display id: annotation1955475
version: 1
top level: BBa_I742145
location: https://synbiohub.org/public/igem/BBa_I742145/annotation1955475/range1955475/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_I742145/annotation1955475/range1955475/1
type: Range
persistent identity: range1955475
display id: range1955475
version: 1
top level: BBa_I742145
direction: direction/forward
start: 3
end: 9
orientation: inline

BBa_K123009
type: ComponentDefinition
persistent identity: BBa K123009
display id: BBa_K123009
version: 1
was generated by: iGEM to SBOL conversion
description: lacIq->BisdB
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: Bisphenolics
owned by: james
owned by: myers
mutable description: This Construct will allow for the detection and degredation of BPA in cells already containing the ER (K123003) biobrick. It works in two systems first. Together K123003 and K123009 produce a NOT gate where when Bisphenol A (BPA), Estrogen or any other estrogen mimic is in the system the product of K123003 dimerizes and attaches to the ERE site of K123009. This stops the production of TetR (R0040) by phsically stoping the Polymerase from transcribing it. This allows the pTet(C0040)promoter to turn on the production of RFP (E1010) for detection as well as BisdA(K123000) and BisdB(K123001)which allow for the degredation of BPA.
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: While BisdA and BisdB have only been shown to degrade BPA we have put in RFP to allow for the detection of other estrogen mimics as well.
star: true
creator: Jason Gardiner
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K123008
component: BBa_E1010
component: BBa_R0040
component: BBa_K123002
sequence annotation: BBa_K123002
sequence annotation: BBa_R0040
sequence annotation: BBa_E1010
sequence annotation: BBa_K123008
sequence: https://synbiohub.org/public/igem/BBa_K123009_sequence/1

BBa_K123008
type: Component
persistent identity: component2267240
display id: component2267240
version: 1
top level: BBa_K123009
definition: BBa_K123008
access: public

BBa_E1010
type: Component
persistent identity: component2267227
display id: component2267227
version: 1
top level: BBa_K123009
definition: mRFP1
access: public

BBa_R0040
type: Component
persistent identity: component2267220
display id: component2267220
version: 1
top level: BBa_K123009
definition: p(tetR)
access: public

BBa_K123002
type: Component
persistent identity: component2267219
display id: component2267219
version: 1
top level: BBa_K123009
definition: BBa_K123002
access: public

BBa_K123002
type: SequenceAnnotation
persistent identity: annotation2267219
display id: annotation2267219
version: 1
top level: BBa_K123009
location: https://synbiohub.org/public/igem/BBa_K123009/annotation2267219/range2267219/1
component: BBa_K123002

https://synbiohub.org/public/igem/BBa_K123009/annotation2267219/range2267219/1
type: Range
persistent identity: range2267219
display id: range2267219
version: 1
top level: BBa_K123009
direction: direction/reverse
start: 1
end: 742
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2267220
display id: annotation2267220
version: 1
top level: BBa_K123009
location: https://synbiohub.org/public/igem/BBa_K123009/annotation2267220/range2267220/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K123009/annotation2267220/range2267220/1
type: Range
persistent identity: range2267220
display id: range2267220
version: 1
top level: BBa_K123009
direction: direction/forward
start: 751
end: 804
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2267227
display id: annotation2267227
version: 1
top level: BBa_K123009
location: https://synbiohub.org/public/igem/BBa_K123009/annotation2267227/range2267227/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K123009/annotation2267227/range2267227/1
type: Range
persistent identity: range2267227
display id: range2267227
version: 1
top level: BBa_K123009
direction: direction/reverse
start: 811
end: 1516
orientation: inline

BBa_K123008
type: SequenceAnnotation
persistent identity: annotation2267240
display id: annotation2267240
version: 1
top level: BBa_K123009
location: https://synbiohub.org/public/igem/BBa_K123009/annotation2267240/range2267240/1
component: BBa_K123008

https://synbiohub.org/public/igem/BBa_K123009/annotation2267240/range2267240/1
type: Range
persistent identity: range2267240
display id: range2267240
version: 1
top level: BBa_K123009
direction: direction/reverse
start: 1525
end: 3266
orientation: inline

BBa_K302001
type: ComponentDefinition
persistent identity: BBa K302001
display id: BBa_K302001
version: 1
was generated by: iGEM to SBOL conversion
description: yneA coding sequence
created: 2010-08-11T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: Genomic DNA ''Bacillus subtilis''
owned by: james
owned by: myers
mutable description: ''Bacillus subtilis'' in response to stress such as DNA damage stops the cells from dividing. This is a part of the SOS response initiated by the accumulation of single stranded DNA from DNA damage or stalled replication. Two proteins are vital for this response: RecA and LexA. RecA forms filaments on ssDNA and promotes the autocleavage of LexA. LexA usually represses the SOS operon. ''dinR'' is homologous to ''lexA'' in ''E. coli'' and is transcribed in the opposite direction of ''yneA''. ''yneA'' stops the formation of ''ftsZ'' ring indirectly. When ''ftsZ'' forms a 30 subunit ring at the midpoint of the cell, it will contract and cause cell division. By expressing ''yneA'' and inhibiting ''ftsZ'' ring formation, the cells will grow filamentous. By inhibiting cell division, yneA allows the DNA damage genes to repair the DNA damage before continuing with the cell division cycle. It is hypothesized that yneA acts through an unknown transmembrane protein to inhibit ftsZ ring formation; we call this/these unknown components ???Blackbox proteins???. As the evidence shows expression of yneA leads to filamentation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _442_
m user id: 0
owner id: 6014
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Rachel May
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: double stop codon
sequence: https://synbiohub.org/public/igem/BBa_K302001_sequence/1

double stop codon
type: SequenceAnnotation
persistent identity: annotation2091504
display id: annotation2091504
version: 1
top level: BBa_K302001
location: https://synbiohub.org/public/igem/BBa_K302001/annotation2091504/range2091504/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K302001/annotation2091504/range2091504/1
type: Range
persistent identity: range2091504
display id: range2091504
version: 1
top level: BBa_K302001
direction: direction/forward
start: 316
end: 321
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_M1999
type: ComponentDefinition
persistent identity: BBa M1999
display id: BBa_M1999
version: 1
was generated by: iGEM to SBOL conversion
description: composite
created: 2012-03-21T12:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: blah
owned by: james
owned by: myers
mutable description: blah
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 12223
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: blah
star: false
creator: David Harris
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_M1998
component: BBa_B0012
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_M1998
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_M1999_sequence/1

BBa_B0010
type: Component
persistent identity: component2170885
display id: component2170885
version: 1
top level: BBa_M1999
definition: BBa_B0010
access: public

BBa_M1998
type: Component
persistent identity: component2170884
display id: component2170884
version: 1
top level: BBa_M1999
definition: BBa_M1998
access: public

BBa_B0012
type: Component
persistent identity: component2170887
display id: component2170887
version: 1
top level: BBa_M1999
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component2170880
display id: component2170880
version: 1
top level: BBa_M1999
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component2170874
display id: component2170874
version: 1
top level: BBa_M1999
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2170874
display id: annotation2170874
version: 1
top level: BBa_M1999
location: https://synbiohub.org/public/igem/BBa_M1999/annotation2170874/range2170874/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_M1999/annotation2170874/range2170874/1
type: Range
persistent identity: range2170874
display id: range2170874
version: 1
top level: BBa_M1999
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2170880
display id: annotation2170880
version: 1
top level: BBa_M1999
location: https://synbiohub.org/public/igem/BBa_M1999/annotation2170880/range2170880/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M1999/annotation2170880/range2170880/1
type: Range
persistent identity: range2170880
display id: range2170880
version: 1
top level: BBa_M1999
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_M1998
type: SequenceAnnotation
persistent identity: annotation2170884
display id: annotation2170884
version: 1
top level: BBa_M1999
location: https://synbiohub.org/public/igem/BBa_M1999/annotation2170884/range2170884/1
component: BBa_M1998

https://synbiohub.org/public/igem/BBa_M1999/annotation2170884/range2170884/1
type: Range
persistent identity: range2170884
display id: range2170884
version: 1
top level: BBa_M1999
direction: direction/reverse
start: 81
end: 766
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2170885
display id: annotation2170885
version: 1
top level: BBa_M1999
location: https://synbiohub.org/public/igem/BBa_M1999/annotation2170885/range2170885/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M1999/annotation2170885/range2170885/1
type: Range
persistent identity: range2170885
display id: range2170885
version: 1
top level: BBa_M1999
direction: direction/forward
start: 775
end: 854
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2170887
display id: annotation2170887
version: 1
top level: BBa_M1999
location: https://synbiohub.org/public/igem/BBa_M1999/annotation2170887/range2170887/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M1999/annotation2170887/range2170887/1
type: Range
persistent identity: range2170887
display id: range2170887
version: 1
top level: BBa_M1999
direction: direction/forward
start: 863
end: 903
orientation: inline

BBa_M1000
type: ComponentDefinition
persistent identity: BBa M1000
display id: BBa_M1000
version: 1
was generated by: iGEM to SBOL conversion
description: Left mosaic end for Tn5 transpososome
created: 2006-03-16T12:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: Synthesis from overlapping oligos
owned by: james
owned by: myers
mutable description: Left end of a linear transpososome sequence containing the 19 bp mosaic end in the forward orientation. The sequence also contains a PvuII cut site which releases the transpososome when cut with PvuII. Constructed compound parts intended for transposition must not contain other PvuII end, or be PCR amplified with blunt end producing enzymes (Pfu, Phusion) at the PvuII cut site between CAG and CTG. This part, and the companion part M1001 provide Biobrick tools to construct Tn5 transpososomes: linear DNA sequences which can be cut in vitro from a plasmid backbone, or created by PCR, combined in vitro with purified Tn5 trasposase protein, electroportated into cells, and finally randomly inserting into the bacterial chromosome. The parts assembled between the two mosaic ends can be delivered, including antibiotic resistance markers or other functional chromosomal insertions.
discontinued: true
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: ;biobrick left end, TA cloning overhang GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ;biobrick right end, TA cloning overhang GTT TCT TCC TGC AGC GGC CGC TAC TAG TA ;mosaic end with cag upstream to insert PvuII cut site cagCTGTCTCTTATACACATCT ;mosaic end reverse complement with ctg upstream to insert PvuII cut site AGATGTGTATAAGAGACAGctg ;mosaic left end in biobrick form construction primer F GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG cagCTGTCTCTTATACACATCT, MEL-F ;mosaic left end in biobrick form construction primer R GTT TCT TCC TGC AGC GGC CGC TAC TAG TA AGATGTGTATAAGAGACAGctg, MEL-R ;mosaic right end in biobrick form construction primer F GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG AGATGTGTATAAGAGACAGctg, MER-F ;mosaic right end in biobrick form construction primer R GTT TCT TCC TGC AGC GGC CGC TAC TAG TA cagCTGTCTCTTATACACATCT, MER-R
star: true
creator: Tom Knight
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: PvuII cut site
sequence annotation: Mosaic End (forward)
sequence: https://synbiohub.org/public/igem/BBa_M1000_sequence/1

PvuII cut site
type: SequenceAnnotation
persistent identity: annotation1825568
display id: annotation1825568
version: 1
top level: BBa_M1000
location: https://synbiohub.org/public/igem/BBa_M1000/annotation1825568/range1825568/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1000/annotation1825568/range1825568/1
type: Range
persistent identity: range1825568
display id: range1825568
version: 1
top level: BBa_M1000
direction: direction/forward
start: 1
end: 6
orientation: inline

Mosaic End (forward)
type: SequenceAnnotation
persistent identity: annotation1825569
display id: annotation1825569
version: 1
top level: BBa_M1000
location: https://synbiohub.org/public/igem/BBa_M1000/annotation1825569/range1825569/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1000/annotation1825569/range1825569/1
type: Range
persistent identity: range1825569
display id: range1825569
version: 1
top level: BBa_M1000
direction: direction/forward
start: 4
end: 22
orientation: inline

BBa_K123008
type: ComponentDefinition
persistent identity: BBa K123008
display id: BBa_K123008
version: 1
was generated by: iGEM to SBOL conversion
description: BisdA+BisdB
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: Bisphenolics
owned by: james
owned by: myers
mutable description: This part when used will allow the production of TetR to increase the production of BisdA and BisdB. When produced BisdA and BisdB will cause the degredation of Bisphenol A.
discontinued: false
dominant: false
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Jason Gardiner
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_R0040
component: BBa_K123001
component: BBa_K123000
sequence annotation: BBa_R0040
sequence annotation: BBa_K123000
sequence annotation: BBa_K123001
sequence: https://synbiohub.org/public/igem/BBa_K123008_sequence/1

BBa_R0040
type: Component
persistent identity: component1992756
display id: component1992756
version: 1
top level: BBa_K123008
definition: p(tetR)
access: public

BBa_K123001
type: Component
persistent identity: component1992767
display id: component1992767
version: 1
top level: BBa_K123008
definition: BisdB
access: public

BBa_R0040
type: Component
persistent identity: component1992762
display id: component1992762
version: 1
top level: BBa_K123008
definition: p(tetR)
access: public

BBa_K123000
type: Component
persistent identity: component1992761
display id: component1992761
version: 1
top level: BBa_K123008
definition: BisdA
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1992756
display id: annotation1992756
version: 1
top level: BBa_K123008
location: https://synbiohub.org/public/igem/BBa_K123008/annotation1992756/range1992756/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K123008/annotation1992756/range1992756/1
type: Range
persistent identity: range1992756
display id: range1992756
version: 1
top level: BBa_K123008
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_K123000
type: SequenceAnnotation
persistent identity: annotation1992761
display id: annotation1992761
version: 1
top level: BBa_K123008
location: https://synbiohub.org/public/igem/BBa_K123008/annotation1992761/range1992761/1
component: BBa_K123000

https://synbiohub.org/public/igem/BBa_K123008/annotation1992761/range1992761/1
type: Range
persistent identity: range1992761
display id: range1992761
version: 1
top level: BBa_K123008
direction: direction/reverse
start: 61
end: 390
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1992762
display id: annotation1992762
version: 1
top level: BBa_K123008
location: https://synbiohub.org/public/igem/BBa_K123008/annotation1992762/range1992762/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K123008/annotation1992762/range1992762/1
type: Range
persistent identity: range1992762
display id: range1992762
version: 1
top level: BBa_K123008
direction: direction/forward
start: 399
end: 452
orientation: inline

BBa_K123001
type: SequenceAnnotation
persistent identity: annotation1992767
display id: annotation1992767
version: 1
top level: BBa_K123008
location: https://synbiohub.org/public/igem/BBa_K123008/annotation1992767/range1992767/1
component: BBa_K123001

https://synbiohub.org/public/igem/BBa_K123008/annotation1992767/range1992767/1
type: Range
persistent identity: range1992767
display id: range1992767
version: 1
top level: BBa_K123008
direction: direction/reverse
start: 459
end: 1742
orientation: inline

BBa_M1998
type: ComponentDefinition
persistent identity: BBa M1998
display id: BBa_M1998
version: 1
was generated by: iGEM to SBOL conversion
description: blah
created: 2012-03-21T12:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: Baccilus blah
owned by: james
owned by: myers
mutable description: blah blah blah
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12223
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: blah blah blah
star: false
is replaced by: BBa_M11107
creator: David Harris
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence annotation: start codon
sequence annotation: cds
sequence annotation: just for fun
sequence: https://synbiohub.org/public/igem/BBa_M1998_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2170800
display id: annotation2170800
version: 1
top level: BBa_M1998
location: https://synbiohub.org/public/igem/BBa_M1998/annotation2170800/range2170800/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1998/annotation2170800/range2170800/1
type: Range
persistent identity: range2170800
display id: range2170800
version: 1
top level: BBa_M1998
direction: direction/forward
start: 1
end: 3
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2170787
display id: annotation2170787
version: 1
top level: BBa_M1998
location: https://synbiohub.org/public/igem/BBa_M1998/annotation2170787/range2170787/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_M1998/annotation2170787/range2170787/1
type: Range
persistent identity: range2170787
display id: range2170787
version: 1
top level: BBa_M1998
direction: direction/forward
start: 1
end: 686
orientation: inline

just for fun
type: SequenceAnnotation
persistent identity: annotation2170793
display id: annotation2170793
version: 1
top level: BBa_M1998
location: https://synbiohub.org/public/igem/BBa_M1998/annotation2170793/range2170793/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1998/annotation2170793/range2170793/1
type: Range
persistent identity: range2170793
display id: range2170793
version: 1
top level: BBa_M1998
direction: direction/forward
start: 380
end: 400
orientation: inline

BisdA
type: ComponentDefinition
persistent identity: BBa K123000
display id: BBa_K123000
version: 1
was generated by: iGEM to SBOL conversion
description: BisdA
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: Sphingomonas sp. strain AO1
owned by: james
owned by: myers
mutable description: When used in conjunction with BisdB this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli by Miho Sasaki1 et al. 2005. Miho Sasaki1, Jun-ichi Maki, Ko-ichi Oshiman, Yoshinobu Matsumura1, and Tetsuaki Tsuchido.2005, Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1. Biodegredation 16: 449-459
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: These parts where designed to include a his tag to make it easier to view enzyme presence
star: true
creator: Jason Gardiner
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K123000_sequence/1

BisdB
type: ComponentDefinition
persistent identity: BBa K123001
display id: BBa_K123001
version: 1
was generated by: iGEM to SBOL conversion
description: BisdB
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: Sphingomonas sp. strain AO1
owned by: james
owned by: myers
mutable description: When used in conjunction with BisdA this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli by Miho Sasaki1 et al. 2005. Miho Sasaki1, Jun-ichi Maki, Ko-ichi Oshiman, Yoshinobu Matsumura1, and Tetsuaki Tsuchido.2005, Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1. Biodegredation 16: 449-459
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: When used with BisdA the degredation of Bisphenol A sharply increases
star: true
creator: Jason Gardiner
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K123001_sequence/1

BBa_M11107
type: ComponentDefinition
persistent identity: BBa M11107
display id: BBa_M11107
version: 1
was generated by: iGEM to SBOL conversion
description: example
created: 2010-04-01T11:00:00Z
modified: 2015-05-08T01:13:53Z
mutable provenance: exampl
owned by: james
owned by: myers
mutable description: example
discontinued: false
dominant: true
group u list: _302_
m user id: 0
owner id: 2425
owning group id: 303
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: exampl
star: false
creator: Charles Miller
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start ATG
sequence: https://synbiohub.org/public/igem/BBa_M11107_sequence/1

start ATG
type: SequenceAnnotation
persistent identity: annotation2067582
display id: annotation2067582
version: 1
top level: BBa_M11107
location: https://synbiohub.org/public/igem/BBa_M11107/annotation2067582/range2067582/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M11107/annotation2067582/range2067582/1
type: Range
persistent identity: range2067582
display id: range2067582
version: 1
top level: BBa_M11107
direction: direction/forward
start: 1
end: 3
orientation: inline

https://synbiohub.org/public/igem/BBa_M1844_sequence/1
type: Sequence
persistent identity: BBa M1844 sequence
display id: BBa_M1844_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggccaacaccgtgtcggccaagaagatgacccgcaagatcgccaagcgtacggcgatcaaccgctcgcgccgttcgcggatgcgtaccttcgtccgcaaggtcgaggaggcgattgcgtccggcgatcagggccaggccctcaccgccctccgcgccgccgagccggagatcatgcgcgctgcccagaacggcatcgttcacaagaacaatgcctcgcggaaggtttcccgcctggccgcccgcgtgaaggccatcgcggcctgatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtactagagccatagcacctatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtcaagacttcgtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1986_sequence/1
type: Sequence
persistent identity: BBa M1986 sequence
display id: BBa_M1986_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccaacaccgtgtcggccaagaagatgacccgcaagatcgccaagcgtacggcgatcaaccgctcgcgccgttcgcggatgcgtaccttcgtccgcaaggtcgaggaggcgattgcgtccggcgatcagggccaggccctcaccgccctccgcgccgccgagccggagatcatgcgcgctgcccagaacggcatcgttcacaagaacaatgcctcgcggaaggtttcccgcctggccgcccgcgtgaaggccatcgcggcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1843_sequence/1
type: Sequence
persistent identity: BBa M1843 sequence
display id: BBa_M1843_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtactagagccatagcacctatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcagctgtctcttatacacatcttactagagccatagcacctatactagagacggagaagcagcgaatactagatggccaacaccgtgtcggccaagaagatgacccgcaagatcgccaagcgtacggcgatcaaccgctcgcgccgttcgcggatgcgtaccttcgtccgcaaggtcgaggaggcgattgcgtccggcgatcagggccaggccctcaccgccctccgcgccgccgagccggagatcatgcgcgctgcccagaacggcatcgttcacaagaacaatgcctcgcggaaggtttcccgcctggccgcccgcgtgaaggccatcgcggcctgatacta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K123002_sequence/1
type: Sequence
persistent identity: BBa K123002 sequence
display id: BBa_K123002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1987_sequence/1
type: Sequence
persistent identity: BBa M1987 sequence
display id: BBa_M1987_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acggagaagcagcgaatactagatggccaacaccgtgtcggccaagaagatgacccgcaagatcgccaagcgtacggcgatcaaccgctcgcgccgttcgcggatgcgtaccttcgtccgcaaggtcgaggaggcgattgcgtccggcgatcagggccaggccctcaccgccctccgcgccgccgagccggagatcatgcgcgctgcccagaacggcatcgttcacaagaacaatgcctcgcggaaggtttcccgcctggccgcccgcgtgaaggccatcgcggcctgatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1872_sequence/1
type: Sequence
persistent identity: BBa M1872 sequence
display id: BBa_M1872_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1877_sequence/1
type: Sequence
persistent identity: BBa M1877 sequence
display id: BBa_M1877_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccatagcaccta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1845_sequence/1
type: Sequence
persistent identity: BBa M1845 sequence
display id: BBa_M1845_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1888_sequence/1
type: Sequence
persistent identity: BBa M1888 sequence
display id: BBa_M1888_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccatagcaccta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1887_sequence/1
type: Sequence
persistent identity: BBa M1887 sequence
display id: BBa_M1887_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccatagcacctatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcagctgtctcttatacacatct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0082_sequence/1
type: Sequence
persistent identity: BBa R0082 sequence
display id: BBa_R0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742145_sequence/1
type: Sequence
persistent identity: BBa I742145 sequence
display id: BBa_I742145_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acggagaagcagcgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K123009_sequence/1
type: Sequence
persistent identity: BBa K123009 sequence
display id: BBa_K123009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggcaaccctcag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K302001_sequence/1
type: Sequence
persistent identity: BBa K302001 sequence
display id: BBa_K302001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcatgagtaaagaatctattatttttgtcggtctgtttacagtgattttgagcgcggttatccttatgctgtcatatacaagcagcggccaagagcttaatcagtatgttaaaatagaagtccagcaaggcgacacactctggtcaattgctgatcaggtagccgatacaaaaaagataaacaaaaatgattttattgaatgggtagctgataaaaatcaacttcaaacctctgatatccagccgggtgatgagttagtgatcccattgaaaaagaagcatcaggatgcatatgaattagcaactgtaagataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1999_sequence/1
type: Sequence
persistent identity: BBa M1999 sequence
display id: BBa_M1999_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1000_sequence/1
type: Sequence
persistent identity: BBa M1000 sequence
display id: BBa_M1000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagctgtctcttatacacatct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K123008_sequence/1
type: Sequence
persistent identity: BBa K123008 sequence
display id: BBa_K123008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggcaaccctcagacactgccggtctttccagacctcgacatcttttcgcccgaatatgcctgcaaccgcgaaaaatacgcagcccgcgcccttagggactatccgctgcacttctataagccgctgaatttgtggatcgtcagcaagcacaaggatgtgcgctcggccttgttcactccgcaggtcttttcttctgtcgcgtttggcctgctccccccgcccgatgatatcgcgccgcgcgtgccggacctttacaccgacgtgcatctgccctcgatggacccgccggagcacaccaagctcagggttcccgtccagcaagccctcctccccggtcggctggtgggtaaggacgaggtcgttcgccggatcgccaacgaacttatcgataccttcatcgacaagggcgaatgcgatctcctccacgatttcagctacaaacttgcgctctacttgatcgtcgatctgctcgggatccccaaggaacgggcggaagattatcatcgctggtccaactgcttcttccagctgttcacccctaaggtaccggagcgcgccgacgcaagattcttcgtgccgatgccggaagaggtactgcggcagaactgggaaggcctggcggaggcgaacgactacctgcgcgaagtcgtcgagaaccttgatcgcaatcccggcaacaacatgctgtcaaacctgttgcagctgcgcgaaccggacggctcacgcacgatcacgatcagcgccaatgtttgcaacgcactggaatttggggcggctggtcatgacaccacggcaacgctgatcgcgcacctcacctatttcgtgctgaccacgccggacctcaaggacactcttaccgaggatccgtcgctgatccccgcggcgatttccgaaacgctgcgccgtcgctgtccggtcgacgggctgttccggcgaacgctgtcggatgtagaactatgcggccagaaaatcgaatccggatcgatcgtctacctcgatctcaccgctgcaaatcttgaccccgatgtattccccgaacctgagacgtttcgcctgaaccgcgacaatatcaaggaaatggtctctttcggcttcggtcgtcacgtctgcgcaggacagtacctgtcccgtatcgaagcaaaagcggcctacgaagaactgatgcgccgtattcccaacatgcgtctcgctgatggtttcaagctggaatacatgccttccgtggcgacgacggttctcaaggggcttccgctggtctgggacaaaaacaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1998_sequence/1
type: Sequence
persistent identity: BBa M1998 sequence
display id: BBa_M1998_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K123000_sequence/1
type: Sequence
persistent identity: BBa K123000 sequence
display id: BBa_K123000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K123001_sequence/1
type: Sequence
persistent identity: BBa K123001 sequence
display id: BBa_K123001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccggcaaccctcagacactgccggtctttccagacctcgacatcttttcgcccgaatatgcctgcaaccgcgaaaaatacgcagcccgcgcccttagggactatccgctgcacttctataagccgctgaatttgtggatcgtcagcaagcacaaggatgtgcgctcggccttgttcactccgcaggtcttttcttctgtcgcgtttggcctgctccccccgcccgatgatatcgcgccgcgcgtgccggacctttacaccgacgtgcatctgccctcgatggacccgccggagcacaccaagctcagggttcccgtccagcaagccctcctccccggtcggctggtgggtaaggacgaggtcgttcgccggatcgccaacgaacttatcgataccttcatcgacaagggcgaatgcgatctcctccacgatttcagctacaaacttgcgctctacttgatcgtcgatctgctcgggatccccaaggaacgggcggaagattatcatcgctggtccaactgcttcttccagctgttcacccctaaggtaccggagcgcgccgacgcaagattcttcgtgccgatgccggaagaggtactgcggcagaactgggaaggcctggcggaggcgaacgactacctgcgcgaagtcgtcgagaaccttgatcgcaatcccggcaacaacatgctgtcaaacctgttgcagctgcgcgaaccggacggctcacgcacgatcacgatcagcgccaatgtttgcaacgcactggaatttggggcggctggtcatgacaccacggcaacgctgatcgcgcacctcacctatttcgtgctgaccacgccggacctcaaggacactcttaccgaggatccgtcgctgatccccgcggcgatttccgaaacgctgcgccgtcgctgtccggtcgacgggctgttccggcgaacgctgtcggatgtagaactatgcggccagaaaatcgaatccggatcgatcgtctacctcgatctcaccgctgcaaatcttgaccccgatgtattccccgaacctgagacgtttcgcctgaaccgcgacaatatcaaggaaatggtctctttcggcttcggtcgtcacgtctgcgcaggacagtacctgtcccgtatcgaagcaaaagcggcctacgaagaactgatgcgccgtattcccaacatgcgtctcgctgatggtttcaagctggaatacatgccttccgtggcgacgacggttctcaaggggcttccgctggtctgggacaaaaacaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M11107_sequence/1
type: Sequence
persistent identity: BBa M11107 sequence
display id: BBa_M11107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z