BBa_M1887
type: ComponentDefinition
persistent identity: BBa M1887
display id: BBa_M1887
version: 1
was generated by: iGEM to SBOL conversion
description: fgf
created: 2012-03-26T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: fgf
owned by: james
owned by: myers
mutable description: gfgf
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12283
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: fgfg
star: false
creator: Elizabeth Martinez
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_M1877
component: BBa_M1000
component: BBa_M1999
sequence annotation: BBa_M1877
sequence annotation: BBa_M1999
sequence annotation: BBa_M1000
sequence: https://synbiohub.org/public/igem/BBa_M1887_sequence/1

BBa_M1877
type: Component
persistent identity: component2171261
display id: component2171261
version: 1
top level: BBa_M1887
definition: BBa_M1877
access: public

BBa_M1000
type: Component
persistent identity: component2171282
display id: component2171282
version: 1
top level: BBa_M1887
definition: BBa_M1000
access: public

BBa_M1999
type: Component
persistent identity: component2171279
display id: component2171279
version: 1
top level: BBa_M1887
definition: BBa_M1999
access: public

BBa_M1877
type: SequenceAnnotation
persistent identity: annotation2171261
display id: annotation2171261
version: 1
top level: BBa_M1887
location: https://synbiohub.org/public/igem/BBa_M1887/annotation2171261/range2171261/1
component: BBa_M1877

https://synbiohub.org/public/igem/BBa_M1887/annotation2171261/range2171261/1
type: Range
persistent identity: range2171261
display id: range2171261
version: 1
top level: BBa_M1887
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_M1999
type: SequenceAnnotation
persistent identity: annotation2171279
display id: annotation2171279
version: 1
top level: BBa_M1887
location: https://synbiohub.org/public/igem/BBa_M1887/annotation2171279/range2171279/1
component: BBa_M1999

https://synbiohub.org/public/igem/BBa_M1887/annotation2171279/range2171279/1
type: Range
persistent identity: range2171279
display id: range2171279
version: 1
top level: BBa_M1887
direction: direction/reverse
start: 21
end: 923
orientation: inline

BBa_M1000
type: SequenceAnnotation
persistent identity: annotation2171282
display id: annotation2171282
version: 1
top level: BBa_M1887
location: https://synbiohub.org/public/igem/BBa_M1887/annotation2171282/range2171282/1
component: BBa_M1000

https://synbiohub.org/public/igem/BBa_M1887/annotation2171282/range2171282/1
type: Range
persistent identity: range2171282
display id: range2171282
version: 1
top level: BBa_M1887
direction: direction/reverse
start: 932
end: 953
orientation: inline

BBa_M1999
type: ComponentDefinition
persistent identity: BBa M1999
display id: BBa_M1999
version: 1
was generated by: iGEM to SBOL conversion
description: composite
created: 2012-03-21T12:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: blah
owned by: james
owned by: myers
mutable description: blah
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 12223
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: blah
star: false
creator: David Harris
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0010
component: BBa_M1998
component: BBa_B0012
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_M1998
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_M1999_sequence/1

BBa_B0010
type: Component
persistent identity: component2170885
display id: component2170885
version: 1
top level: BBa_M1999
definition: BBa_B0010
access: public

BBa_M1998
type: Component
persistent identity: component2170884
display id: component2170884
version: 1
top level: BBa_M1999
definition: BBa_M1998
access: public

BBa_B0012
type: Component
persistent identity: component2170887
display id: component2170887
version: 1
top level: BBa_M1999
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component2170880
display id: component2170880
version: 1
top level: BBa_M1999
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component2170874
display id: component2170874
version: 1
top level: BBa_M1999
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2170874
display id: annotation2170874
version: 1
top level: BBa_M1999
location: https://synbiohub.org/public/igem/BBa_M1999/annotation2170874/range2170874/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_M1999/annotation2170874/range2170874/1
type: Range
persistent identity: range2170874
display id: range2170874
version: 1
top level: BBa_M1999
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2170880
display id: annotation2170880
version: 1
top level: BBa_M1999
location: https://synbiohub.org/public/igem/BBa_M1999/annotation2170880/range2170880/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M1999/annotation2170880/range2170880/1
type: Range
persistent identity: range2170880
display id: range2170880
version: 1
top level: BBa_M1999
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_M1998
type: SequenceAnnotation
persistent identity: annotation2170884
display id: annotation2170884
version: 1
top level: BBa_M1999
location: https://synbiohub.org/public/igem/BBa_M1999/annotation2170884/range2170884/1
component: BBa_M1998

https://synbiohub.org/public/igem/BBa_M1999/annotation2170884/range2170884/1
type: Range
persistent identity: range2170884
display id: range2170884
version: 1
top level: BBa_M1999
direction: direction/reverse
start: 81
end: 766
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2170885
display id: annotation2170885
version: 1
top level: BBa_M1999
location: https://synbiohub.org/public/igem/BBa_M1999/annotation2170885/range2170885/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M1999/annotation2170885/range2170885/1
type: Range
persistent identity: range2170885
display id: range2170885
version: 1
top level: BBa_M1999
direction: direction/forward
start: 775
end: 854
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2170887
display id: annotation2170887
version: 1
top level: BBa_M1999
location: https://synbiohub.org/public/igem/BBa_M1999/annotation2170887/range2170887/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M1999/annotation2170887/range2170887/1
type: Range
persistent identity: range2170887
display id: range2170887
version: 1
top level: BBa_M1999
direction: direction/forward
start: 863
end: 903
orientation: inline

BBa_M1877
type: ComponentDefinition
persistent identity: BBa M1877
display id: BBa_M1877
version: 1
was generated by: iGEM to SBOL conversion
description: human insulin
created: 2012-03-26T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: dfadf
owned by: james
owned by: myers
mutable description: zedfd
discontinued: false
dominant: true
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12283
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: dfdf
star: false
creator: Elizabeth Martinez
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence annotation: insulin start
sequence annotation: coding sequence
sequence annotation: insulin
sequence annotation: insulin stop
sequence: https://synbiohub.org/public/igem/BBa_M1877_sequence/1

insulin start
type: SequenceAnnotation
persistent identity: annotation2171242
display id: annotation2171242
version: 1
top level: BBa_M1877
location: https://synbiohub.org/public/igem/BBa_M1877/annotation2171242/range2171242/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1877/annotation2171242/range2171242/1
type: Range
persistent identity: range2171242
display id: range2171242
version: 1
top level: BBa_M1877
direction: direction/forward
start: 1
end: 1
orientation: inline

coding sequence
type: SequenceAnnotation
persistent identity: annotation2171241
display id: annotation2171241
version: 1
top level: BBa_M1877
location: https://synbiohub.org/public/igem/BBa_M1877/annotation2171241/range2171241/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_M1877/annotation2171241/range2171241/1
type: Range
persistent identity: range2171241
display id: range2171241
version: 1
top level: BBa_M1877
direction: direction/forward
start: 1
end: 12
orientation: inline

insulin
type: SequenceAnnotation
persistent identity: annotation2171243
display id: annotation2171243
version: 1
top level: BBa_M1877
location: https://synbiohub.org/public/igem/BBa_M1877/annotation2171243/range2171243/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_M1877/annotation2171243/range2171243/1
type: Range
persistent identity: range2171243
display id: range2171243
version: 1
top level: BBa_M1877
direction: direction/forward
start: 1
end: 12
orientation: inline

insulin stop
type: SequenceAnnotation
persistent identity: annotation2171248
display id: annotation2171248
version: 1
top level: BBa_M1877
location: https://synbiohub.org/public/igem/BBa_M1877/annotation2171248/range2171248/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1877/annotation2171248/range2171248/1
type: Range
persistent identity: range2171248
display id: range2171248
version: 1
top level: BBa_M1877
direction: direction/forward
start: 12
end: 12
orientation: inline

BBa_M1000
type: ComponentDefinition
persistent identity: BBa M1000
display id: BBa_M1000
version: 1
was generated by: iGEM to SBOL conversion
description: Left mosaic end for Tn5 transpososome
created: 2006-03-16T12:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: Synthesis from overlapping oligos
owned by: james
owned by: myers
mutable description: Left end of a linear transpososome sequence containing the 19 bp mosaic end in the forward orientation. The sequence also contains a PvuII cut site which releases the transpososome when cut with PvuII. Constructed compound parts intended for transposition must not contain other PvuII end, or be PCR amplified with blunt end producing enzymes (Pfu, Phusion) at the PvuII cut site between CAG and CTG. This part, and the companion part M1001 provide Biobrick tools to construct Tn5 transpososomes: linear DNA sequences which can be cut in vitro from a plasmid backbone, or created by PCR, combined in vitro with purified Tn5 trasposase protein, electroportated into cells, and finally randomly inserting into the bacterial chromosome. The parts assembled between the two mosaic ends can be delivered, including antibiotic resistance markers or other functional chromosomal insertions.
discontinued: true
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: ;biobrick left end, TA cloning overhang GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ;biobrick right end, TA cloning overhang GTT TCT TCC TGC AGC GGC CGC TAC TAG TA ;mosaic end with cag upstream to insert PvuII cut site cagCTGTCTCTTATACACATCT ;mosaic end reverse complement with ctg upstream to insert PvuII cut site AGATGTGTATAAGAGACAGctg ;mosaic left end in biobrick form construction primer F GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG cagCTGTCTCTTATACACATCT, MEL-F ;mosaic left end in biobrick form construction primer R GTT TCT TCC TGC AGC GGC CGC TAC TAG TA AGATGTGTATAAGAGACAGctg, MEL-R ;mosaic right end in biobrick form construction primer F GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG AGATGTGTATAAGAGACAGctg, MER-F ;mosaic right end in biobrick form construction primer R GTT TCT TCC TGC AGC GGC CGC TAC TAG TA cagCTGTCTCTTATACACATCT, MER-R
star: true
creator: Tom Knight
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: PvuII cut site
sequence annotation: Mosaic End (forward)
sequence: https://synbiohub.org/public/igem/BBa_M1000_sequence/1

PvuII cut site
type: SequenceAnnotation
persistent identity: annotation1825568
display id: annotation1825568
version: 1
top level: BBa_M1000
location: https://synbiohub.org/public/igem/BBa_M1000/annotation1825568/range1825568/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1000/annotation1825568/range1825568/1
type: Range
persistent identity: range1825568
display id: range1825568
version: 1
top level: BBa_M1000
direction: direction/forward
start: 1
end: 6
orientation: inline

Mosaic End (forward)
type: SequenceAnnotation
persistent identity: annotation1825569
display id: annotation1825569
version: 1
top level: BBa_M1000
location: https://synbiohub.org/public/igem/BBa_M1000/annotation1825569/range1825569/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1000/annotation1825569/range1825569/1
type: Range
persistent identity: range1825569
display id: range1825569
version: 1
top level: BBa_M1000
direction: direction/forward
start: 4
end: 22
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_M1998
type: ComponentDefinition
persistent identity: BBa M1998
display id: BBa_M1998
version: 1
was generated by: iGEM to SBOL conversion
description: blah
created: 2012-03-21T12:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: Baccilus blah
owned by: james
owned by: myers
mutable description: blah blah blah
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12223
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: blah blah blah
star: false
is replaced by: BBa_M11107
creator: David Harris
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence annotation: cds
sequence annotation: start codon
sequence annotation: just for fun
sequence: https://synbiohub.org/public/igem/BBa_M1998_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2170787
display id: annotation2170787
version: 1
top level: BBa_M1998
location: https://synbiohub.org/public/igem/BBa_M1998/annotation2170787/range2170787/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_M1998/annotation2170787/range2170787/1
type: Range
persistent identity: range2170787
display id: range2170787
version: 1
top level: BBa_M1998
direction: direction/forward
start: 1
end: 686
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2170800
display id: annotation2170800
version: 1
top level: BBa_M1998
location: https://synbiohub.org/public/igem/BBa_M1998/annotation2170800/range2170800/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M1998/annotation2170800/range2170800/1
type: Range
persistent identity: range2170800
display id: range2170800
version: 1
top level: BBa_M1998
direction: direction/forward
start: 1
end: 3
orientation: inline

just for fun
type: SequenceAnnotation
persistent identity: annotation2170793
display id: annotation2170793
version: 1
top level: BBa_M1998
location: https://synbiohub.org/public/igem/BBa_M1998/annotation2170793/range2170793/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1998/annotation2170793/range2170793/1
type: Range
persistent identity: range2170793
display id: range2170793
version: 1
top level: BBa_M1998
direction: direction/forward
start: 380
end: 400
orientation: inline

BBa_M11107
type: ComponentDefinition
persistent identity: BBa M11107
display id: BBa_M11107
version: 1
was generated by: iGEM to SBOL conversion
description: example
created: 2010-04-01T11:00:00Z
modified: 2015-05-08T01:13:53Z
mutable provenance: exampl
owned by: james
owned by: myers
mutable description: example
discontinued: false
dominant: true
group u list: _302_
m user id: 0
owner id: 2425
owning group id: 303
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: exampl
star: false
creator: Charles Miller
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start ATG
sequence: https://synbiohub.org/public/igem/BBa_M11107_sequence/1

start ATG
type: SequenceAnnotation
persistent identity: annotation2067582
display id: annotation2067582
version: 1
top level: BBa_M11107
location: https://synbiohub.org/public/igem/BBa_M11107/annotation2067582/range2067582/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M11107/annotation2067582/range2067582/1
type: Range
persistent identity: range2067582
display id: range2067582
version: 1
top level: BBa_M11107
direction: direction/forward
start: 1
end: 3
orientation: inline

https://synbiohub.org/public/igem/BBa_M1887_sequence/1
type: Sequence
persistent identity: BBa M1887 sequence
display id: BBa_M1887_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccatagcacctatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcagctgtctcttatacacatct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1999_sequence/1
type: Sequence
persistent identity: BBa M1999 sequence
display id: BBa_M1999_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1877_sequence/1
type: Sequence
persistent identity: BBa M1877 sequence
display id: BBa_M1877_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccatagcaccta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1000_sequence/1
type: Sequence
persistent identity: BBa M1000 sequence
display id: BBa_M1000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagctgtctcttatacacatct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M11107_sequence/1
type: Sequence
persistent identity: BBa M11107 sequence
display id: BBa_M11107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1998_sequence/1
type: Sequence
persistent identity: BBa M1998 sequence
display id: BBa_M1998_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctgcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z