LOCUS BBa_M1987 4006 bp DNA linear 17-May-2024 DEFINITION Composite with rpsT gene ACCESSION BBa_M1987 VERSION BBa_M1987.1 FEATURES Location/Qualifiers RBS 3..9 /label=rbs RBS 1..16 /label=BBa_I742145 misc_feature 23..25 /label=Start codon misc_feature 287..289 /label=Stop Codon CDS 26..286 /label=Open reading frame CDS 23..289 /label=BBa_M1986 misc_feature 298..334 /label=P(Lac) IQ promoter 300..305 /label=-35 promoter 323..328 /label=-10 misc_feature 298..334 /label=BBa_I14032 CDS 344..960 /label=tetR misc_feature 961..994 /label=SsrA misc_feature 341..1000 /label=BBa_C0040 misc_feature 1001..1025 /label=barcode misc_feature 340..354 /label=ERE promoter 298..1039 /label=BBa_K123002 misc_feature 1048..1066 /label=TetR 1 misc_feature 1048..1101 /label=BBa_R0040 promoter 1067..1072 /label=-35 misc_feature 1073..1091 /label=TetR 2 promoter 1090..1095 /label=-10 promoter 1048..1101 /label=BBa_R0040 CDS 1108..1782 /label=mrfp1 misc_feature 1789..1813 /label=Help:Barcodes CDS 1108..1813 /label=BBa_E1010 misc_feature 1822..1840 /label=TetR 1 misc_feature 1822..1875 /label=BBa_R0040 promoter 1841..1846 /label=-35 misc_feature 1847..1865 /label=TetR 2 promoter 1864..1869 /label=-10 promoter 1822..1875 /label=BBa_R0040 CDS 1882..2211 /label=BBa_K123000 misc_feature 2220..2238 /label=TetR 1 misc_feature 2220..2273 /label=BBa_R0040 promoter 2239..2244 /label=-35 misc_feature 2245..2263 /label=TetR 2 promoter 2262..2267 /label=-10 promoter 2220..2273 /label=BBa_R0040 CDS 2280..3563 /label=BBa_K123001 misc_feature 1822..3563 /label=BBa_K123008 misc_feature 298..3563 /label=BBa_K123009 misc_feature 3885..3890 /label=double stop codon CDS 3570..3890 /label=BBa_K302001 misc_feature 3911..3928 /label=C1 OmpR misc_feature 3932..3949 /label=C2 OmpR misc_feature 3952..3969 /label=C3 OmpR promoter 3973..3978 /label=-35 promoter 3996..4001 /label=-10 promoter 3899..4006 /label=BBa_R0082 ORIGIN 1 acggagaagc agcgaatact agatggccaa caccgtgtcg gccaagaaga tgacccgcaa 61 gatcgccaag cgtacggcga tcaaccgctc gcgccgttcg cggatgcgta ccttcgtccg 121 caaggtcgag gaggcgattg cgtccggcga tcagggccag gccctcaccg ccctccgcgc 181 cgccgagccg gagatcatgc gcgctgccca gaacggcatc gttcacaaga acaatgcctc 241 gcggaaggtt tcccgcctgg ccgcccgcgt gaaggccatc gcggcctgat actagagtgg 301 tgcaaaacct ttcgcggtat ggcatgatag cgcctactag aggtcagagt gaccatgtcc 361 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc 421 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat 481 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg 541 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac 601 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt 661 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa 721 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta 781 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact 841 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca 901 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa 961 tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga 1021 tagtgctagt gtagatcact actagagtcc ctatcagtga tagagattga catccctatc 1081 agtgatagag atactgagca ctactagatg gcttcctccg aagacgttat caaagagttc 1141 atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt 1201 gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt 1261 ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct 1321 tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc 1381 aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc 1441 tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc 1501 gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac 1561 ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt 1621 cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt 1681 gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt 1741 gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc 1801 tagtgtagat cgctactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat 1861 agagatactg agcactacta gatggccggc cctcatatcc aagtgactac ccgcgatggg 1921 gagatacgcg aactcgacgt cgcagcctcc gggttcctga tggaagccct tcgcgacgcc 1981 aatatcgacg gcgtcgaggc gatgtgcggc ggatgctgct cctgcgcgac ctgccacgtc 2041 tacatcgacg ctgctcccgc cgggaccctg ccgccggtct cctccgacga ggagatgctg 2101 ctttccggcc tggtctcgac ccccggacgg tcgcggctct cctgccaaat tccggtcacg 2161 gccgaactgg atggccttaa gctcacgatc ccgccggact ccaccggtta atactagagt 2221 ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag cactactaga 2281 tggccggcaa ccctcagaca ctgccggtct ttccagacct cgacatcttt tcgcccgaat 2341 atgcctgcaa ccgcgaaaaa tacgcagccc gcgcccttag ggactatccg ctgcacttct 2401 ataagccgct gaatttgtgg atcgtcagca agcacaagga tgtgcgctcg gccttgttca 2461 ctccgcaggt cttttcttct gtcgcgtttg gcctgctccc cccgcccgat gatatcgcgc 2521 cgcgcgtgcc ggacctttac accgacgtgc atctgccctc gatggacccg ccggagcaca 2581 ccaagctcag ggttcccgtc cagcaagccc tcctccccgg tcggctggtg ggtaaggacg 2641 aggtcgttcg ccggatcgcc aacgaactta tcgatacctt catcgacaag ggcgaatgcg 2701 atctcctcca cgatttcagc tacaaacttg cgctctactt gatcgtcgat ctgctcggga 2761 tccccaagga acgggcggaa gattatcatc gctggtccaa ctgcttcttc cagctgttca 2821 cccctaaggt accggagcgc gccgacgcaa gattcttcgt gccgatgccg gaagaggtac 2881 tgcggcagaa ctgggaaggc ctggcggagg cgaacgacta cctgcgcgaa gtcgtcgaga 2941 accttgatcg caatcccggc aacaacatgc tgtcaaacct gttgcagctg cgcgaaccgg 3001 acggctcacg cacgatcacg atcagcgcca atgtttgcaa cgcactggaa tttggggcgg 3061 ctggtcatga caccacggca acgctgatcg cgcacctcac ctatttcgtg ctgaccacgc 3121 cggacctcaa ggacactctt accgaggatc cgtcgctgat ccccgcggcg atttccgaaa 3181 cgctgcgccg tcgctgtccg gtcgacgggc tgttccggcg aacgctgtcg gatgtagaac 3241 tatgcggcca gaaaatcgaa tccggatcga tcgtctacct cgatctcacc gctgcaaatc 3301 ttgaccccga tgtattcccc gaacctgaga cgtttcgcct gaaccgcgac aatatcaagg 3361 aaatggtctc tttcggcttc ggtcgtcacg tctgcgcagg acagtacctg tcccgtatcg 3421 aagcaaaagc ggcctacgaa gaactgatgc gccgtattcc caacatgcgt ctcgctgatg 3481 gtttcaagct ggaatacatg ccttccgtgg cgacgacggt tctcaagggg cttccgctgg 3541 tctgggacaa aaacaccggt taatactaga tgatcatgag taaagaatct attatttttg 3601 tcggtctgtt tacagtgatt ttgagcgcgg ttatccttat gctgtcatat acaagcagcg 3661 gccaagagct taatcagtat gttaaaatag aagtccagca aggcgacaca ctctggtcaa 3721 ttgctgatca ggtagccgat acaaaaaaga taaacaaaaa tgattttatt gaatgggtag 3781 ctgataaaaa tcaacttcaa acctctgata tccagccggg tgatgagtta gtgatcccat 3841 tgaaaaagaa gcatcaggat gcatatgaat tagcaactgt aagataataa tactagagtc 3901 ccttgcattt acattttgaa acatctatag cgataaatga aacatcttaa aagttttagt 3961 atcatattcg tgttggatta ttctgcattt ttggggagaa tggact //