##gff-version 3
##sequence-region BBa_M1987 1 4006
BBa_M1987	.	CDS	3570	3890	.	+	0	ID=BBa_K302001;Name=BBa_K302001
BBa_M1987	.	stop_codon	3885	3890	.	+	0	ID=annotation2091504;Name=double stop codon;Parent=BBa_K302001
BBa_M1987	.	CDS	23	289	.	+	0	ID=BBa_M1986;Name=BBa_M1986
BBa_M1987	.	CDS	26	286	.	+	0	ID=annotation2171189;Name=Open reading frame;Parent=BBa_M1986
BBa_M1987	.	stop_codon	287	289	.	+	0	ID=annotation2171188;Name=Stop Codon;Parent=BBa_M1986
BBa_M1987	.	start_codon	23	25	.	+	0	ID=annotation2171187;Name=Start codon;Parent=BBa_M1986
BBa_M1987	.	engineered_region	298	3563	.	+	0	ID=BBa_K123009;Name=BBa_K123009
BBa_M1987	.	promoter	298	1039	.	+	0	ID=BBa_K123002;Name=BBa_K123002;Parent=BBa_K123009
BBa_M1987	.	non_covalent_binding_site	340	354	.	+	0	ID=annotation1992733;Name=ERE;Parent=BBa_K123002
BBa_M1987	.	operator	298	334	.	+	0	ID=annotation1992717;Name=P(Lac) IQ;Parent=BBa_K123002
BBa_M1987	.	CDS	344	960	.	+	0	ID=annotation1992721;Name=tetR;Parent=BBa_K123002
BBa_M1987	.	tag	961	994	.	+	0	ID=annotation1992722;Name=SsrA;Parent=BBa_K123002
BBa_M1987	.	engineered_region	298	334	.	+	0	ID=annotation1992720;Name=BBa_I14032;Parent=BBa_K123002
BBa_M1987	.	engineered_region	1001	1025	.	+	0	ID=annotation1992724;Name=barcode;Parent=BBa_K123002
BBa_M1987	.	promoter	300	305	.	+	0	ID=annotation1992718;Name=-35;Parent=BBa_K123002
BBa_M1987	.	promoter	323	328	.	+	0	ID=annotation1992719;Name=-10;Parent=BBa_K123002
BBa_M1987	.	engineered_region	341	1000	.	+	0	ID=annotation1992723;Name=BBa_C0040;Parent=BBa_K123002
BBa_M1987	.	engineered_region	1822	3563	.	+	0	ID=BBa_K123008;Name=BBa_K123008;Parent=BBa_K123009
BBa_M1987	.	CDS	1882	2211	.	+	0	ID=BBa_K123000;Name=BBa_K123000;Parent=BBa_K123008
BBa_M1987	.	CDS	2280	3563	.	+	0	ID=BBa_K123001;Name=BBa_K123001;Parent=BBa_K123008
BBa_M1987	.	promoter	2220	2273	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K123008
BBa_M1987	.	non_covalent_binding_site	2245	2263	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_M1987	.	engineered_region	2220	2273	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_M1987	.	non_covalent_binding_site	2220	2238	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_M1987	.	promoter	2262	2267	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_M1987	.	promoter	2239	2244	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_M1987	.	promoter	1822	1875	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K123008
BBa_M1987	.	non_covalent_binding_site	1847	1865	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_M1987	.	engineered_region	1822	1875	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_M1987	.	non_covalent_binding_site	1822	1840	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_M1987	.	promoter	1864	1869	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_M1987	.	promoter	1841	1846	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_M1987	.	CDS	1108	1813	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K123009
BBa_M1987	.	CDS	1108	1782	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_M1987	.	engineered_tag	1789	1813	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_M1987	.	promoter	1048	1101	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K123009
BBa_M1987	.	non_covalent_binding_site	1073	1091	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_M1987	.	engineered_region	1048	1101	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_M1987	.	non_covalent_binding_site	1048	1066	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_M1987	.	promoter	1090	1095	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_M1987	.	promoter	1067	1072	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_M1987	.	ribosome_entry_site	1	16	.	+	0	ID=BBa_I742145;Name=BBa_I742145
BBa_M1987	.	ribosome_entry_site	3	9	.	+	0	ID=annotation1955475;Name=rbs;Parent=BBa_I742145
BBa_M1987	.	promoter	3899	4006	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_M1987	.	non_covalent_binding_site	3911	3928	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_M1987	.	non_covalent_binding_site	3932	3949	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_M1987	.	non_covalent_binding_site	3952	3969	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_M1987	.	promoter	3996	4001	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_M1987	.	promoter	3973	3978	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
>BBa_M1987
acggagaagcagcgaatactagatggccaacaccgtgtcggccaagaagatgacccgcaagatcgccaagcgtacggcg
atcaaccgctcgcgccgttcgcggatgcgtaccttcgtccgcaaggtcgaggaggcgattgcgtccggcgatcagggcc
aggccctcaccgccctccgcgccgccgagccggagatcatgcgcgctgcccagaacggcatcgttcacaagaacaatgc
ctcgcggaaggtttcccgcctggccgcccgcgtgaaggccatcgcggcctgatactagagtggtgcaaaacctttcgcg
gtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcatt
agagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctaca
ttgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcact
tttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtca
tcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagccttt
ttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtat
tggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgaca
agctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaa
aaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgct
agtgtagatcactactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactacta
gatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgag
ttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtc
cgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacat
cccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtt
accgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccg
acggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaa
aggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaa
aaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcg
ttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctact
agagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggccctcat
atccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcg
acgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgc
tcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcg
cggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaat
actagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggcaac
cctcagacactgccggtctttccagacctcgacatcttttcgcccgaatatgcctgcaaccgcgaaaaatacgcagccc
gcgcccttagggactatccgctgcacttctataagccgctgaatttgtggatcgtcagcaagcacaaggatgtgcgctc
ggccttgttcactccgcaggtcttttcttctgtcgcgtttggcctgctccccccgcccgatgatatcgcgccgcgcgtg
ccggacctttacaccgacgtgcatctgccctcgatggacccgccggagcacaccaagctcagggttcccgtccagcaag
ccctcctccccggtcggctggtgggtaaggacgaggtcgttcgccggatcgccaacgaacttatcgataccttcatcga
caagggcgaatgcgatctcctccacgatttcagctacaaacttgcgctctacttgatcgtcgatctgctcgggatcccc
aaggaacgggcggaagattatcatcgctggtccaactgcttcttccagctgttcacccctaaggtaccggagcgcgccg
acgcaagattcttcgtgccgatgccggaagaggtactgcggcagaactgggaaggcctggcggaggcgaacgactacct
gcgcgaagtcgtcgagaaccttgatcgcaatcccggcaacaacatgctgtcaaacctgttgcagctgcgcgaaccggac
ggctcacgcacgatcacgatcagcgccaatgtttgcaacgcactggaatttggggcggctggtcatgacaccacggcaa
cgctgatcgcgcacctcacctatttcgtgctgaccacgccggacctcaaggacactcttaccgaggatccgtcgctgat
ccccgcggcgatttccgaaacgctgcgccgtcgctgtccggtcgacgggctgttccggcgaacgctgtcggatgtagaa
ctatgcggccagaaaatcgaatccggatcgatcgtctacctcgatctcaccgctgcaaatcttgaccccgatgtattcc
ccgaacctgagacgtttcgcctgaaccgcgacaatatcaaggaaatggtctctttcggcttcggtcgtcacgtctgcgc
aggacagtacctgtcccgtatcgaagcaaaagcggcctacgaagaactgatgcgccgtattcccaacatgcgtctcgct
gatggtttcaagctggaatacatgccttccgtggcgacgacggttctcaaggggcttccgctggtctgggacaaaaaca
ccggttaatactagatgatcatgagtaaagaatctattatttttgtcggtctgtttacagtgattttgagcgcggttat
ccttatgctgtcatatacaagcagcggccaagagcttaatcagtatgttaaaatagaagtccagcaaggcgacacactc
tggtcaattgctgatcaggtagccgatacaaaaaagataaacaaaaatgattttattgaatgggtagctgataaaaatc
aacttcaaacctctgatatccagccgggtgatgagttagtgatcccattgaaaaagaagcatcaggatgcatatgaatt
agcaactgtaagataataatactagagtcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaa
aagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact