BBa_M1987
type: ComponentDefinition
persistent identity: BBa M1987
display id: BBa_M1987
version: 1
was generated by: iGEM to SBOL conversion
description: Composite with rpsT gene
created: 2012-03-26T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: the source of the gene is from Methylobacterium chloromethanicum. Other parts are from the registry.
owned by: james
owned by: myers
mutable description: the rpsT gene with a promoter-RBS- and two TC stops added
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 12223
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: I just found different ribosome binding sites and Tc stops from the registry. The promoter is for the glucose controlled ompC porin gene. The RBS is the native rbs for the sam5 gene of Saccharothrix espanaensis The first stop stops RNA polymerase from transcribing the TetR gene. The second codes for a gene that inhibits cell division
star: false
creator: David Harris
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K302001
component: BBa_K123009
component: BBa_R0082
component: BBa_M1986
component: BBa_I742145
sequence annotation: BBa_I742145
sequence annotation: BBa_M1986
sequence annotation: BBa_K123009
sequence annotation: BBa_K302001
sequence annotation: BBa_R0082
sequence: https://synbiohub.org/public/igem/BBa_M1987_sequence/1

BBa_K302001
type: Component
persistent identity: component2295772
display id: component2295772
version: 1
top level: BBa_M1987
definition: BBa_K302001
access: public

BBa_K123009
type: Component
persistent identity: component2295770
display id: component2295770
version: 1
top level: BBa_M1987
definition: BBa_K123009
access: public

BBa_R0082
type: Component
persistent identity: component2295778
display id: component2295778
version: 1
top level: BBa_M1987
definition: OmpR
access: public

BBa_M1986
type: Component
persistent identity: component2295738
display id: component2295738
version: 1
top level: BBa_M1987
definition: BBa_M1986
access: public

BBa_I742145
type: Component
persistent identity: component2295734
display id: component2295734
version: 1
top level: BBa_M1987
definition: BBa_I742145
access: public

BBa_I742145
type: SequenceAnnotation
persistent identity: annotation2295734
display id: annotation2295734
version: 1
top level: BBa_M1987
location: https://synbiohub.org/public/igem/BBa_M1987/annotation2295734/range2295734/1
component: BBa_I742145

https://synbiohub.org/public/igem/BBa_M1987/annotation2295734/range2295734/1
type: Range
persistent identity: range2295734
display id: range2295734
version: 1
top level: BBa_M1987
direction: direction/reverse
start: 1
end: 16
orientation: inline

BBa_M1986
type: SequenceAnnotation
persistent identity: annotation2295738
display id: annotation2295738
version: 1
top level: BBa_M1987
location: https://synbiohub.org/public/igem/BBa_M1987/annotation2295738/range2295738/1
component: BBa_M1986

https://synbiohub.org/public/igem/BBa_M1987/annotation2295738/range2295738/1
type: Range
persistent identity: range2295738
display id: range2295738
version: 1
top level: BBa_M1987
direction: direction/reverse
start: 23
end: 289
orientation: inline

BBa_K123009
type: SequenceAnnotation
persistent identity: annotation2295770
display id: annotation2295770
version: 1
top level: BBa_M1987
location: https://synbiohub.org/public/igem/BBa_M1987/annotation2295770/range2295770/1
component: BBa_K123009

https://synbiohub.org/public/igem/BBa_M1987/annotation2295770/range2295770/1
type: Range
persistent identity: range2295770
display id: range2295770
version: 1
top level: BBa_M1987
direction: direction/reverse
start: 298
end: 3563
orientation: inline

BBa_K302001
type: SequenceAnnotation
persistent identity: annotation2295772
display id: annotation2295772
version: 1
top level: BBa_M1987
location: https://synbiohub.org/public/igem/BBa_M1987/annotation2295772/range2295772/1
component: BBa_K302001

https://synbiohub.org/public/igem/BBa_M1987/annotation2295772/range2295772/1
type: Range
persistent identity: range2295772
display id: range2295772
version: 1
top level: BBa_M1987
direction: direction/reverse
start: 3570
end: 3890
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2295778
display id: annotation2295778
version: 1
top level: BBa_M1987
location: https://synbiohub.org/public/igem/BBa_M1987/annotation2295778/range2295778/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_M1987/annotation2295778/range2295778/1
type: Range
persistent identity: range2295778
display id: range2295778
version: 1
top level: BBa_M1987
direction: direction/reverse
start: 3899
end: 4006
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
top level: OmpR
direction: direction/forward
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
top level: OmpR
direction: direction/forward
start: 54
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
top level: OmpR
direction: direction/forward
start: 75
end: 80
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
top level: OmpR
direction: direction/forward
start: 98
end: 103
orientation: inline

BBa_I742145
type: ComponentDefinition
persistent identity: BBa I742145
display id: BBa_I742145
version: 1
was generated by: iGEM to SBOL conversion
description: sam5 native rbs
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Saccharothrix espanaensis DSM 44229
owned by: james
owned by: myers
mutable description: This is the native ribosome binding site for sam5 (4-coumarate-3-hydroxylase) of the bacterium Saccharothrix espanaensis.
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is a virtual part to be added to coding sequence I742141.
star: false
creator: Chris French
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_I742145_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1955475
display id: annotation1955475
version: 1
top level: BBa_I742145
location: https://synbiohub.org/public/igem/BBa_I742145/annotation1955475/range1955475/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_I742145/annotation1955475/range1955475/1
type: Range
persistent identity: range1955475
display id: range1955475
version: 1
top level: BBa_I742145
direction: direction/forward
start: 3
end: 9
orientation: inline

BBa_M1986
type: ComponentDefinition
persistent identity: BBa M1986
display id: BBa_M1986
version: 1
was generated by: iGEM to SBOL conversion
description: rpsT gene from Methylobacterium Chloromethanicum
created: 2012-03-26T11:00:00Z
modified: 2015-05-08T01:13:58Z
mutable provenance: Methylobacterium Chloromethanicum. The sequence comes from the site http://www.ncbi.nlm.nih.gov/nuccore/218528082?from=359&to=625
owned by: james
owned by: myers
mutable description: The rpsT gene binds directly to the 16s rRNA and induces post translational inhibition of arginine and ornithine decarboxylase
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 12223
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: I copied and pasted the sequence from NCBI.
star: false
creator: David Harris
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: Open reading frame
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M1986_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2171187
display id: annotation2171187
version: 1
top level: BBa_M1986
location: https://synbiohub.org/public/igem/BBa_M1986/annotation2171187/range2171187/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M1986/annotation2171187/range2171187/1
type: Range
persistent identity: range2171187
display id: range2171187
version: 1
top level: BBa_M1986
direction: direction/forward
start: 1
end: 3
orientation: inline

Open reading frame
type: SequenceAnnotation
persistent identity: annotation2171189
display id: annotation2171189
version: 1
top level: BBa_M1986
location: https://synbiohub.org/public/igem/BBa_M1986/annotation2171189/range2171189/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_M1986/annotation2171189/range2171189/1
type: Range
persistent identity: range2171189
display id: range2171189
version: 1
top level: BBa_M1986
direction: direction/forward
start: 4
end: 264
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2171188
display id: annotation2171188
version: 1
top level: BBa_M1986
location: https://synbiohub.org/public/igem/BBa_M1986/annotation2171188/range2171188/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1986/annotation2171188/range2171188/1
type: Range
persistent identity: range2171188
display id: range2171188
version: 1
top level: BBa_M1986
direction: direction/forward
start: 265
end: 267
orientation: inline

BBa_K123009
type: ComponentDefinition
persistent identity: BBa K123009
display id: BBa_K123009
version: 1
was generated by: iGEM to SBOL conversion
description: lacIq->BisdB
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: Bisphenolics
owned by: james
owned by: myers
mutable description: This Construct will allow for the detection and degredation of BPA in cells already containing the ER (K123003) biobrick. It works in two systems first. Together K123003 and K123009 produce a NOT gate where when Bisphenol A (BPA), Estrogen or any other estrogen mimic is in the system the product of K123003 dimerizes and attaches to the ERE site of K123009. This stops the production of TetR (R0040) by phsically stoping the Polymerase from transcribing it. This allows the pTet(C0040)promoter to turn on the production of RFP (E1010) for detection as well as BisdA(K123000) and BisdB(K123001)which allow for the degredation of BPA.
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: While BisdA and BisdB have only been shown to degrade BPA we have put in RFP to allow for the detection of other estrogen mimics as well.
star: true
creator: Jason Gardiner
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K123008
component: BBa_E1010
component: BBa_R0040
component: BBa_K123002
sequence annotation: BBa_K123002
sequence annotation: BBa_R0040
sequence annotation: BBa_E1010
sequence annotation: BBa_K123008
sequence: https://synbiohub.org/public/igem/BBa_K123009_sequence/1

BBa_K123008
type: Component
persistent identity: component2267240
display id: component2267240
version: 1
top level: BBa_K123009
definition: BBa_K123008
access: public

BBa_E1010
type: Component
persistent identity: component2267227
display id: component2267227
version: 1
top level: BBa_K123009
definition: mRFP1
access: public

BBa_R0040
type: Component
persistent identity: component2267220
display id: component2267220
version: 1
top level: BBa_K123009
definition: p(tetR)
access: public

BBa_K123002
type: Component
persistent identity: component2267219
display id: component2267219
version: 1
top level: BBa_K123009
definition: BBa_K123002
access: public

BBa_K123002
type: SequenceAnnotation
persistent identity: annotation2267219
display id: annotation2267219
version: 1
top level: BBa_K123009
location: https://synbiohub.org/public/igem/BBa_K123009/annotation2267219/range2267219/1
component: BBa_K123002

https://synbiohub.org/public/igem/BBa_K123009/annotation2267219/range2267219/1
type: Range
persistent identity: range2267219
display id: range2267219
version: 1
top level: BBa_K123009
direction: direction/reverse
start: 1
end: 742
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2267220
display id: annotation2267220
version: 1
top level: BBa_K123009
location: https://synbiohub.org/public/igem/BBa_K123009/annotation2267220/range2267220/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K123009/annotation2267220/range2267220/1
type: Range
persistent identity: range2267220
display id: range2267220
version: 1
top level: BBa_K123009
direction: direction/forward
start: 751
end: 804
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2267227
display id: annotation2267227
version: 1
top level: BBa_K123009
location: https://synbiohub.org/public/igem/BBa_K123009/annotation2267227/range2267227/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K123009/annotation2267227/range2267227/1
type: Range
persistent identity: range2267227
display id: range2267227
version: 1
top level: BBa_K123009
direction: direction/reverse
start: 811
end: 1516
orientation: inline

BBa_K123008
type: SequenceAnnotation
persistent identity: annotation2267240
display id: annotation2267240
version: 1
top level: BBa_K123009
location: https://synbiohub.org/public/igem/BBa_K123009/annotation2267240/range2267240/1
component: BBa_K123008

https://synbiohub.org/public/igem/BBa_K123009/annotation2267240/range2267240/1
type: Range
persistent identity: range2267240
display id: range2267240
version: 1
top level: BBa_K123009
direction: direction/reverse
start: 1525
end: 3266
orientation: inline

BBa_K302001
type: ComponentDefinition
persistent identity: BBa K302001
display id: BBa_K302001
version: 1
was generated by: iGEM to SBOL conversion
description: yneA coding sequence
created: 2010-08-11T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: Genomic DNA ''Bacillus subtilis''
owned by: james
owned by: myers
mutable description: ''Bacillus subtilis'' in response to stress such as DNA damage stops the cells from dividing. This is a part of the SOS response initiated by the accumulation of single stranded DNA from DNA damage or stalled replication. Two proteins are vital for this response: RecA and LexA. RecA forms filaments on ssDNA and promotes the autocleavage of LexA. LexA usually represses the SOS operon. ''dinR'' is homologous to ''lexA'' in ''E. coli'' and is transcribed in the opposite direction of ''yneA''. ''yneA'' stops the formation of ''ftsZ'' ring indirectly. When ''ftsZ'' forms a 30 subunit ring at the midpoint of the cell, it will contract and cause cell division. By expressing ''yneA'' and inhibiting ''ftsZ'' ring formation, the cells will grow filamentous. By inhibiting cell division, yneA allows the DNA damage genes to repair the DNA damage before continuing with the cell division cycle. It is hypothesized that yneA acts through an unknown transmembrane protein to inhibit ftsZ ring formation; we call this/these unknown components ???Blackbox proteins???. As the evidence shows expression of yneA leads to filamentation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _442_
m user id: 0
owner id: 6014
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Rachel May
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: double stop codon
sequence: https://synbiohub.org/public/igem/BBa_K302001_sequence/1

double stop codon
type: SequenceAnnotation
persistent identity: annotation2091504
display id: annotation2091504
version: 1
top level: BBa_K302001
location: https://synbiohub.org/public/igem/BBa_K302001/annotation2091504/range2091504/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K302001/annotation2091504/range2091504/1
type: Range
persistent identity: range2091504
display id: range2091504
version: 1
top level: BBa_K302001
direction: direction/forward
start: 316
end: 321
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K123002
type: ComponentDefinition
persistent identity: BBa K123002
display id: BBa_K123002
version: 1
was generated by: iGEM to SBOL conversion
description: LacIQ ERE TetR
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: The Estrogen Responsive Element was taken from the Human Estrogen Responsive Element
owned by: james
owned by: myers
mutable description: This composite part contains LacIQ (BBa_I14032) and Estrogen Receptor Element (ERE) and TetR (BBa_C0040). The Estrogen Responsive element is to be used in conjunction with ER (Estrogen Receptor). This design involves ER binding to the ERE and disrupting the constituative promoter LacIQ from producing TetR via physically getting in the way of the polymerase.
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: There is some data that suggests the ER is toxic to the cell. However in our experiments while we did see a decrease in growth rate we did not find ER to be fatal to the cell.
star: true
creator: Jason Gardiner
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_I14032
sequence annotation: P(Lac) IQ
sequence annotation: -35
sequence annotation: -10
sequence annotation: ERE
sequence annotation: BBa_C0040
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: barcode
sequence: https://synbiohub.org/public/igem/BBa_K123002_sequence/1

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation1992720
display id: annotation1992720
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992720/range1992720/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K123002/annotation1992720/range1992720/1
type: Range
persistent identity: range1992720
display id: range1992720
version: 1
top level: BBa_K123002
direction: direction/reverse
start: 1
end: 37
orientation: inline

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1992717
display id: annotation1992717
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992717/range1992717/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K123002/annotation1992717/range1992717/1
type: Range
persistent identity: range1992717
display id: range1992717
version: 1
top level: BBa_K123002
direction: direction/forward
start: 1
end: 37
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1992718
display id: annotation1992718
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992718/range1992718/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K123002/annotation1992718/range1992718/1
type: Range
persistent identity: range1992718
display id: range1992718
version: 1
top level: BBa_K123002
direction: direction/forward
start: 3
end: 8
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1992719
display id: annotation1992719
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992719/range1992719/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K123002/annotation1992719/range1992719/1
type: Range
persistent identity: range1992719
display id: range1992719
version: 1
top level: BBa_K123002
direction: direction/forward
start: 26
end: 31
orientation: inline

ERE
type: SequenceAnnotation
persistent identity: annotation1992733
display id: annotation1992733
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992733/range1992733/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K123002/annotation1992733/range1992733/1
type: Range
persistent identity: range1992733
display id: range1992733
version: 1
top level: BBa_K123002
direction: direction/forward
start: 43
end: 57
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation1992723
display id: annotation1992723
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992723/range1992723/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K123002/annotation1992723/range1992723/1
type: Range
persistent identity: range1992723
display id: range1992723
version: 1
top level: BBa_K123002
direction: direction/reverse
start: 44
end: 703
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation1992721
display id: annotation1992721
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992721/range1992721/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K123002/annotation1992721/range1992721/1
type: Range
persistent identity: range1992721
display id: range1992721
version: 1
top level: BBa_K123002
direction: direction/forward
start: 47
end: 663
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation1992722
display id: annotation1992722
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992722/range1992722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K123002/annotation1992722/range1992722/1
type: Range
persistent identity: range1992722
display id: range1992722
version: 1
top level: BBa_K123002
direction: direction/forward
start: 664
end: 697
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1992724
display id: annotation1992724
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992724/range1992724/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K123002/annotation1992724/range1992724/1
type: Range
persistent identity: range1992724
display id: range1992724
version: 1
top level: BBa_K123002
direction: direction/reverse
start: 704
end: 728
orientation: inline

BBa_K123008
type: ComponentDefinition
persistent identity: BBa K123008
display id: BBa_K123008
version: 1
was generated by: iGEM to SBOL conversion
description: BisdA+BisdB
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: Bisphenolics
owned by: james
owned by: myers
mutable description: This part when used will allow the production of TetR to increase the production of BisdA and BisdB. When produced BisdA and BisdB will cause the degredation of Bisphenol A.
discontinued: false
dominant: false
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Jason Gardiner
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_R0040
component: BBa_K123001
component: BBa_K123000
sequence annotation: BBa_R0040
sequence annotation: BBa_K123000
sequence annotation: BBa_K123001
sequence: https://synbiohub.org/public/igem/BBa_K123008_sequence/1

BBa_R0040
type: Component
persistent identity: component1992756
display id: component1992756
version: 1
top level: BBa_K123008
definition: p(tetR)
access: public

BBa_K123001
type: Component
persistent identity: component1992767
display id: component1992767
version: 1
top level: BBa_K123008
definition: BisdB
access: public

BBa_R0040
type: Component
persistent identity: component1992762
display id: component1992762
version: 1
top level: BBa_K123008
definition: p(tetR)
access: public

BBa_K123000
type: Component
persistent identity: component1992761
display id: component1992761
version: 1
top level: BBa_K123008
definition: BisdA
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1992756
display id: annotation1992756
version: 1
top level: BBa_K123008
location: https://synbiohub.org/public/igem/BBa_K123008/annotation1992756/range1992756/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K123008/annotation1992756/range1992756/1
type: Range
persistent identity: range1992756
display id: range1992756
version: 1
top level: BBa_K123008
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_K123000
type: SequenceAnnotation
persistent identity: annotation1992761
display id: annotation1992761
version: 1
top level: BBa_K123008
location: https://synbiohub.org/public/igem/BBa_K123008/annotation1992761/range1992761/1
component: BBa_K123000

https://synbiohub.org/public/igem/BBa_K123008/annotation1992761/range1992761/1
type: Range
persistent identity: range1992761
display id: range1992761
version: 1
top level: BBa_K123008
direction: direction/reverse
start: 61
end: 390
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1992762
display id: annotation1992762
version: 1
top level: BBa_K123008
location: https://synbiohub.org/public/igem/BBa_K123008/annotation1992762/range1992762/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K123008/annotation1992762/range1992762/1
type: Range
persistent identity: range1992762
display id: range1992762
version: 1
top level: BBa_K123008
direction: direction/forward
start: 399
end: 452
orientation: inline

BBa_K123001
type: SequenceAnnotation
persistent identity: annotation1992767
display id: annotation1992767
version: 1
top level: BBa_K123008
location: https://synbiohub.org/public/igem/BBa_K123008/annotation1992767/range1992767/1
component: BBa_K123001

https://synbiohub.org/public/igem/BBa_K123008/annotation1992767/range1992767/1
type: Range
persistent identity: range1992767
display id: range1992767
version: 1
top level: BBa_K123008
direction: direction/reverse
start: 459
end: 1742
orientation: inline

BisdA
type: ComponentDefinition
persistent identity: BBa K123000
display id: BBa_K123000
version: 1
was generated by: iGEM to SBOL conversion
description: BisdA
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: Sphingomonas sp. strain AO1
owned by: james
owned by: myers
mutable description: When used in conjunction with BisdB this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli by Miho Sasaki1 et al. 2005. Miho Sasaki1, Jun-ichi Maki, Ko-ichi Oshiman, Yoshinobu Matsumura1, and Tetsuaki Tsuchido.2005, Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1. Biodegredation 16: 449-459
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: These parts where designed to include a his tag to make it easier to view enzyme presence
star: true
creator: Jason Gardiner
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K123000_sequence/1

BisdB
type: ComponentDefinition
persistent identity: BBa K123001
display id: BBa_K123001
version: 1
was generated by: iGEM to SBOL conversion
description: BisdB
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: Sphingomonas sp. strain AO1
owned by: james
owned by: myers
mutable description: When used in conjunction with BisdA this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli by Miho Sasaki1 et al. 2005. Miho Sasaki1, Jun-ichi Maki, Ko-ichi Oshiman, Yoshinobu Matsumura1, and Tetsuaki Tsuchido.2005, Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1. Biodegredation 16: 449-459
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: When used with BisdA the degredation of Bisphenol A sharply increases
star: true
creator: Jason Gardiner
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K123001_sequence/1

https://synbiohub.org/public/igem/BBa_M1987_sequence/1
type: Sequence
persistent identity: BBa M1987 sequence
display id: BBa_M1987_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acggagaagcagcgaatactagatggccaacaccgtgtcggccaagaagatgacccgcaagatcgccaagcgtacggcgatcaaccgctcgcgccgttcgcggatgcgtaccttcgtccgcaaggtcgaggaggcgattgcgtccggcgatcagggccaggccctcaccgccctccgcgccgccgagccggagatcatgcgcgctgcccagaacggcatcgttcacaagaacaatgcctcgcggaaggtttcccgcctggccgcccgcgtgaaggccatcgcggcctgatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0082_sequence/1
type: Sequence
persistent identity: BBa R0082 sequence
display id: BBa_R0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742145_sequence/1
type: Sequence
persistent identity: BBa I742145 sequence
display id: BBa_I742145_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acggagaagcagcgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1986_sequence/1
type: Sequence
persistent identity: BBa M1986 sequence
display id: BBa_M1986_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccaacaccgtgtcggccaagaagatgacccgcaagatcgccaagcgtacggcgatcaaccgctcgcgccgttcgcggatgcgtaccttcgtccgcaaggtcgaggaggcgattgcgtccggcgatcagggccaggccctcaccgccctccgcgccgccgagccggagatcatgcgcgctgcccagaacggcatcgttcacaagaacaatgcctcgcggaaggtttcccgcctggccgcccgcgtgaaggccatcgcggcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K123009_sequence/1
type: Sequence
persistent identity: BBa K123009 sequence
display id: BBa_K123009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggcaaccctcag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K302001_sequence/1
type: Sequence
persistent identity: BBa K302001 sequence
display id: BBa_K302001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcatgagtaaagaatctattatttttgtcggtctgtttacagtgattttgagcgcggttatccttatgctgtcatatacaagcagcggccaagagcttaatcagtatgttaaaatagaagtccagcaaggcgacacactctggtcaattgctgatcaggtagccgatacaaaaaagataaacaaaaatgattttattgaatgggtagctgataaaaatcaacttcaaacctctgatatccagccgggtgatgagttagtgatcccattgaaaaagaagcatcaggatgcatatgaattagcaactgtaagataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K123002_sequence/1
type: Sequence
persistent identity: BBa K123002 sequence
display id: BBa_K123002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K123008_sequence/1
type: Sequence
persistent identity: BBa K123008 sequence
display id: BBa_K123008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggcaaccctcagacactgccggtctttccagacctcgacatcttttcgcccgaatatgcctgcaaccgcgaaaaatacgcagcccgcgcccttagggactatccgctgcacttctataagccgctgaatttgtggatcgtcagcaagcacaaggatgtgcgctcggccttgttcactccgcaggtcttttcttctgtcgcgtttggcctgctccccccgcccgatgatatcgcgccgcgcgtgccggacctttacaccgacgtgcatctgccctcgatggacccgccggagcacaccaagctcagggttcccgtccagcaagccctcctccccggtcggctggtgggtaaggacgaggtcgttcgccggatcgccaacgaacttatcgataccttcatcgacaagggcgaatgcgatctcctccacgatttcagctacaaacttgcgctctacttgatcgtcgatctgctcgggatccccaaggaacgggcggaagattatcatcgctggtccaactgcttcttccagctgttcacccctaaggtaccggagcgcgccgacgcaagattcttcgtgccgatgccggaagaggtactgcggcagaactgggaaggcctggcggaggcgaacgactacctgcgcgaagtcgtcgagaaccttgatcgcaatcccggcaacaacatgctgtcaaacctgttgcagctgcgcgaaccggacggctcacgcacgatcacgatcagcgccaatgtttgcaacgcactggaatttggggcggctggtcatgacaccacggcaacgctgatcgcgcacctcacctatttcgtgctgaccacgccggacctcaaggacactcttaccgaggatccgtcgctgatccccgcggcgatttccgaaacgctgcgccgtcgctgtccggtcgacgggctgttccggcgaacgctgtcggatgtagaactatgcggccagaaaatcgaatccggatcgatcgtctacctcgatctcaccgctgcaaatcttgaccccgatgtattccccgaacctgagacgtttcgcctgaaccgcgacaatatcaaggaaatggtctctttcggcttcggtcgtcacgtctgcgcaggacagtacctgtcccgtatcgaagcaaaagcggcctacgaagaactgatgcgccgtattcccaacatgcgtctcgctgatggtttcaagctggaatacatgccttccgtggcgacgacggttctcaaggggcttccgctggtctgggacaaaaacaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K123000_sequence/1
type: Sequence
persistent identity: BBa K123000 sequence
display id: BBa_K123000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K123001_sequence/1
type: Sequence
persistent identity: BBa K123001 sequence
display id: BBa_K123001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccggcaaccctcagacactgccggtctttccagacctcgacatcttttcgcccgaatatgcctgcaaccgcgaaaaatacgcagcccgcgcccttagggactatccgctgcacttctataagccgctgaatttgtggatcgtcagcaagcacaaggatgtgcgctcggccttgttcactccgcaggtcttttcttctgtcgcgtttggcctgctccccccgcccgatgatatcgcgccgcgcgtgccggacctttacaccgacgtgcatctgccctcgatggacccgccggagcacaccaagctcagggttcccgtccagcaagccctcctccccggtcggctggtgggtaaggacgaggtcgttcgccggatcgccaacgaacttatcgataccttcatcgacaagggcgaatgcgatctcctccacgatttcagctacaaacttgcgctctacttgatcgtcgatctgctcgggatccccaaggaacgggcggaagattatcatcgctggtccaactgcttcttccagctgttcacccctaaggtaccggagcgcgccgacgcaagattcttcgtgccgatgccggaagaggtactgcggcagaactgggaaggcctggcggaggcgaacgactacctgcgcgaagtcgtcgagaaccttgatcgcaatcccggcaacaacatgctgtcaaacctgttgcagctgcgcgaaccggacggctcacgcacgatcacgatcagcgccaatgtttgcaacgcactggaatttggggcggctggtcatgacaccacggcaacgctgatcgcgcacctcacctatttcgtgctgaccacgccggacctcaaggacactcttaccgaggatccgtcgctgatccccgcggcgatttccgaaacgctgcgccgtcgctgtccggtcgacgggctgttccggcgaacgctgtcggatgtagaactatgcggccagaaaatcgaatccggatcgatcgtctacctcgatctcaccgctgcaaatcttgaccccgatgtattccccgaacctgagacgtttcgcctgaaccgcgacaatatcaaggaaatggtctctttcggcttcggtcgtcacgtctgcgcaggacagtacctgtcccgtatcgaagcaaaagcggcctacgaagaactgatgcgccgtattcccaacatgcgtctcgctgatggtttcaagctggaatacatgccttccgtggcgacgacggttctcaaggggcttccgctggtctgggacaaaaacaccggttaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z