LOCUS BBa_M1988 5596 bp DNA linear 26-Nov-2024 DEFINITION Composite with rpsT gene revised ACCESSION BBa_M1988 VERSION BBa_M1988.1 FEATURES Location/Qualifiers CDS 387..1103 /label=GFP misc_feature 1628..1675 /label=FRT-site promoter 5068..5096 /label=Amp_R promoter CDS 4166..5026 /label=Ampicillin Resistance Gene misc_feature 3392..4011 /label=pBR322_origin misc_feature 1695..2702 /label=Hygromycine Resistance Gene polyA_site 1117..1344 /label=SV40 terminator misc_feature 171..191 /label=BBB prefix promoter 191..355 /label=JeT promoter misc_feature 359..379 /label=BBB suffix RBS 379..389 /label=Kozak sequence misc_feature 1..5162 /label=BBa_K203100 RBS 5173..5179 /label=rbs RBS 5171..5186 /label=BBa_I742145 misc_feature 5193..5195 /label=Start codon misc_feature 5457..5459 /label=Stop Codon CDS 5196..5456 /label=Open reading frame CDS 5193..5459 /label=BBa_M1986 stem_loop 5479..5522 /label=stem_loop misc_feature 5468..5547 /label=BBa_B0010 terminator 5468..5547 /label=BBa_B0010 stem_loop 5563..5582 /label=T7 TE misc_feature 5589..5589 /label=stop polyA_site 5583..5596 /label=polya misc_feature 5556..5596 /label=BBa_B0012 terminator 5556..5596 /label=BBa_B0012 ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattggtga gaattcgcgg 181 ccgcactagt gggcggagtt agggcggagc caatcagcgt gcgccgttcc gaaagttgcc 241 ttttatggct gggcggaagc ttagaatggg cggtgaacgc cgatgattat ataaggacgc 301 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt 361 gctagcgcgg ccgcctgcag gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg 421 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 481 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 541 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct 601 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 661 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 721 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 781 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 841 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 901 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 961 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc 1021 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 1081 tcggcatgga cgagctgtac aagtaatgat cagcctcgac tgtgccttct agttgccagc 1141 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 1201 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 1261 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 1321 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg 1381 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 1441 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 1501 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 1561 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 1621 gtacctagaa gttcctattc cgaagttcct attctctaga aagtatagga acttccttgg 1681 ccaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca 1741 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg 1801 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc 1861 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg 1921 gggagttcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc 1981 aagacctgcc tgaaaccgaa ctgcccgctg ttctacagcc ggtcgcggag gccatggatg 2041 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa 2101 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc 2161 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc 2221 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct 2281 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga 2341 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt 2401 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc 2461 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg 2521 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag 2581 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct 2641 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg 2701 aatagcacgt actacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 2761 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 2821 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 2881 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 2941 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat 3001 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 3061 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 3121 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 3181 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 3241 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 3301 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 3361 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 3421 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 3481 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 3541 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 3601 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 3661 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 3721 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 3781 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 3841 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 3901 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 3961 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 4021 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 4081 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 4141 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 4201 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 4261 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 4321 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 4381 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 4441 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 4501 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 4561 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 4621 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 4681 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 4741 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 4801 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 4861 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 4921 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 4981 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 5041 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 5101 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 5161 tctactagag acggagaagc agcgaatact agatggccaa caccgtgtcg gccaagaaga 5221 tgacccgcaa gatcgccaag cgtacggcga tcaaccgctc gcgccgttcg cggatgcgta 5281 ccttcgtccg caaggtcgag gaggcgattg cgtccggcga tcagggccag gccctcaccg 5341 ccctccgcgc cgccgagccg gagatcatgc gcgctgccca gaacggcatc gttcacaaga 5401 acaatgcctc gcggaaggtt tcccgcctgg ccgcccgcgt gaaggccatc gcggcctgat 5461 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 5521 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 5581 cctttctgcg tttata //