##gff-version 3
##sequence-region BBa_M1988 1 5596
BBa_M1988	.	CDS	5193	5459	.	+	0	ID=BBa_M1986;Name=BBa_M1986
BBa_M1988	.	stop_codon	5457	5459	.	+	0	ID=annotation2171188;Name=Stop Codon;Parent=BBa_M1986
BBa_M1988	.	start_codon	5193	5195	.	+	0	ID=annotation2171187;Name=Start codon;Parent=BBa_M1986
BBa_M1988	.	CDS	5196	5456	.	+	0	ID=annotation2171189;Name=Open reading frame;Parent=BBa_M1986
BBa_M1988	.	terminator	5556	5596	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_M1988	.	stem_loop	5563	5582	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1988	.	stop_codon	5589	5589	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1988	.	polyA_site	5583	5596	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1988	.	engineered_region	5556	5596	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1988	.	ribosome_entry_site	5171	5186	.	+	0	ID=BBa_I742145;Name=BBa_I742145
BBa_M1988	.	ribosome_entry_site	5173	5179	.	+	0	ID=annotation1955475;Name=rbs;Parent=BBa_I742145
BBa_M1988	.	terminator	5468	5547	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_M1988	.	stem_loop	5479	5522	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1988	.	engineered_region	5468	5547	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1988	.	plasmid_vector	1	5162	.	+	0	ID=BBa_K203100;Name=BBa_K203100
BBa_M1988	.	sequence_feature	1695	2702	.	+	0	ID=annotation2041653;Name=Hygromycine Resistance Gene;Parent=BBa_K203100
BBa_M1988	.	ribosome_entry_site	379	389	.	+	0	ID=annotation2041658;Name=Kozak sequence;Parent=BBa_K203100
BBa_M1988	.	CDS	387	1103	.	+	0	ID=annotation2041648;Name=GFP;Parent=BBa_K203100
BBa_M1988	.	polyA_site	1117	1344	.	+	0	ID=annotation2041654;Name=SV40 terminator;Parent=BBa_K203100
BBa_M1988	.	promoter	191	355	.	+	0	ID=annotation2041656;Name=JeT promoter;Parent=BBa_K203100
BBa_M1988	.	sequence_feature	3392	4011	.	+	0	ID=annotation2041652;Name=pBR322_origin;Parent=BBa_K203100
BBa_M1988	.	promoter	5068	5096	.	+	0	ID=annotation2041650;Name=Amp_R promoter;Parent=BBa_K203100
BBa_M1988	.	sequence_feature	171	191	.	+	0	ID=annotation2041655;Name=BBB prefix;Parent=BBa_K203100
BBa_M1988	.	sequence_feature	1628	1675	.	+	0	ID=annotation2041649;Name=FRT-site;Parent=BBa_K203100
BBa_M1988	.	CDS	4166	5026	.	+	0	ID=annotation2041651;Name=Ampicillin Resistance Gene;Parent=BBa_K203100
BBa_M1988	.	sequence_feature	359	379	.	+	0	ID=annotation2041657;Name=BBB suffix;Parent=BBa_K203100
>BBa_M1988
gacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagta
tctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgacc
gacaattggtgagaattcgcggccgcactagtgggcggagttagggcggagccaatcagcgtgcgccgttccgaaagtt
gccttttatggctgggcggaagcttagaatgggcggtgaacgccgatgattatataaggacgcgccgggtgtggcacag
ctagttccgtcgcagccgggatttgggtcgcggttcttgtttgtgctagcgcggccgcctgcaggccaccatggtgagc
aagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcg
tgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgt
gccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcac
gacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaaga
cccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacgg
caacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggc
atcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacaccc
ccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacga
gaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa
tgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaag
gtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggg
gggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatg
gcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgg
gtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctt
tctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacgg
cacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctag
aaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcga
cagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatat
gtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcc
cgattccggaagtgcttgacattggggagttcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcac
gttgcaagacctgcctgaaaccgaactgcccgctgttctacagccggtcgcggaggccatggatgcgatcgctgcggcc
gatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatat
gcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctct
cgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtc
ctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgcca
acatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgc
aggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttc
gatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcg
cccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcg
tccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatc
gttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgttta
ttgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctag
ttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatca
tggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgta
aagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacct
gtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcg
ctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatcca
cagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgtt
gctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccg
acaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccg
gatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgta
ggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgt
cttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatg
taggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctct
gctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttt
tttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacg
ctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaa
ttaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgag
gcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacggg
agggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaa
ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgg
gaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgct
cgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaa
agcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagca
ctgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgag
aatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaa
agtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaa
cccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaa
atgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcat
ttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcaca
tttccccgaaaagtgccacctgacgtctactagagacggagaagcagcgaatactagatggccaacaccgtgtcggcca
agaagatgacccgcaagatcgccaagcgtacggcgatcaaccgctcgcgccgttcgcggatgcgtaccttcgtccgcaa
ggtcgaggaggcgattgcgtccggcgatcagggccaggccctcaccgccctccgcgccgccgagccggagatcatgcgc
gctgcccagaacggcatcgttcacaagaacaatgcctcgcggaaggtttcccgcctggccgcccgcgtgaaggccatcg
cggcctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata