LOCUS       BBa_M1994                903 bp    DNA     linear       29-Nov-2024
DEFINITION  phasin temp
ACCESSION   BBa_M1994
VERSION     BBa_M1994.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..766
                     /label=Coding sequence
     misc_feature    81..83
                     /label=start
     misc_feature    764..766
                     /label=stop
     misc_feature    81..766
                     /label=BBa_M1993
     stem_loop       786..829
                     /label=stem_loop
     misc_feature    775..854
                     /label=BBa_B0010
     terminator      775..854
                     /label=BBa_B0010
     stem_loop       870..889
                     /label=T7 TE
     misc_feature    896..896
                     /label=stop
     polyA_site      890..903
                     /label=polya
     misc_feature    863..903
                     /label=BBa_B0012
     terminator      863..903
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatcctca ccccggaaca agttgcagca gcgcaaaagg
      121 ccaacctcga aacgctgttc ggcctgacca ccaaggcgtt tgaaggcgtc gaaaagctcg
      181 tcgagctgaa cctgcaggtc gtcaagactt cgttcgcaga aggcgttgac aacgccaaga
      241 aggcgctgtc ggccaaggac gcacaggaac tgctggccat ccaggccgca gccgtgcagc
      301 cggttgccga aaagaccctg gcctacaccc gccacctgta tgaaatcgct tcggaaaccc
      361 agagcgagtt caccaaggta gccgaggctc aactggccga aggctcgaag aacgtgcaag
      421 cgctggtcga gaacctcgcc aagaacgccc cggccggttc ggaatcgacc gtggccatcg
      481 tgaagtcggc gatctccgct gccaacaacg cctacgagtc ggtgcagaag gcgaccaagc
      541 aagcggtcga aatcgctgaa accaacttcc aggctgcggc tacggctgcc accaaggctg
      601 cccagcaagc cagcgccacg gcccgtacgg ccacggcaaa gaagacgacg gctgcctgat
      661 aactgcctgc gttgaagatg gaccggctgc ggccggtccg ttggcaaagc atatcgacgc
      721 ctggcgtttg cggtgtgttt tgccaacgat gaaggtagtg ccctgatact agagccaggc
      781 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      841 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      901 ata
//