LOCUS       BBa_M30000               624 bp    DNA     linear       21-Dec-2024
DEFINITION  OmpR:lacZ reporter
ACCESSION   BBa_M30000
VERSION     BBa_M30000.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     misc_feature    121..124
                     /label=conserved
     RBS             117..128
                     /label=BBa_B0034
     CDS             135..482
                     /label=lacZ alpha
     misc_feature    215..215
                     /label=T
     misc_feature    221..221
                     /label=A
     misc_feature    242..242
                     /label=C
     misc_feature    212..212
                     /label=C
     misc_feature    245..245
                     /label=G
     promoter        160..169
                     /label=T3 promoter
     promoter        307..325
                     /label=T7 promoter
     misc_feature    333..482
                     /label=lacZ gene fragment
     misc_feature    135..149
                     /label=lacZ gene fragment
     misc_feature    135..487
                     /label=BBa_E0033
     stem_loop       507..550
                     /label=stem_loop
     misc_feature    496..575
                     /label=BBa_B0010
     terminator      496..575
                     /label=BBa_B0010
     stem_loop       591..610
                     /label=T7 TE
     misc_feature    617..617
                     /label=stop
     polyA_site      611..624
                     /label=polya
     misc_feature    584..624
                     /label=BBa_B0012
     terminator      584..624
                     /label=BBa_B0012
     terminator      496..624
                     /label=BBa_B0015
     misc_feature    117..624
                     /label=BBa_I2012
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag
      121 aggagaaata ctagatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
      181 acaaaagctg gagctccacc gcggtggcgg cagcactaga gctagtggat cccccgggct
      241 gtagaaattc gatatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca
      301 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
      361 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
      421 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
      481 ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      541 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      601 cgggtgggcc tttctgcgtt tata
//