LOCUS       BBa_M30008              1914 bp    DNA     linear       14-May-2024
DEFINITION  -- No description --
ACCESSION   BBa_M30008
VERSION     BBa_M30008.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     misc_feature    121..124
                     /label=conserved
     RBS             117..128
                     /label=BBa_B0034
     CDS             135..782
                     /label=cII p22
     misc_feature    783..815
                     /label=LVA
     misc_feature    816..821
                     /label=stop
     misc_feature    135..821
                     /label=BBa_C0053
     misc_feature    822..846
                     /label=Help:Barcodes
     CDS             135..821
                     /label=BBa_C0053
     stem_loop       866..909
                     /label=stem_loop
     misc_feature    855..934
                     /label=BBa_B0010
     terminator      855..934
                     /label=BBa_B0010
     stem_loop       950..969
                     /label=T7 TE
     misc_feature    976..976
                     /label=stop
     polyA_site      970..983
                     /label=polya
     misc_feature    943..983
                     /label=BBa_B0012
     terminator      943..983
                     /label=BBa_B0012
     misc_feature    992..994
                     /label=OR3
     misc_feature    1002..1019
                     /label=OR2
     misc_feature    1025..1042
                     /label=OR1
     promoter        1009..1014
                     /label=-35
     promoter        999..1004
                     /label=-35
     promoter        1021..1026
                     /label=-10
     misc_feature    992..1045
                     /label=BBa_R0053
     promoter        992..1045
                     /label=BBa_R0053
     misc_feature    1058..1061
                     /label=conserved
     RBS             1054..1065
                     /label=BBa_B0034
     CDS             1072..1746
                     /label=mrfp1
     misc_feature    1753..1777
                     /label=Help:Barcodes
     CDS             1072..1752
                     /label=BBa_E1010
     stem_loop       1797..1840
                     /label=stem_loop
     misc_feature    1786..1865
                     /label=BBa_B0010
     terminator      1786..1865
                     /label=BBa_B0010
     stem_loop       1881..1900
                     /label=T7 TE
     misc_feature    1907..1907
                     /label=stop
     polyA_site      1901..1914
                     /label=polya
     misc_feature    1874..1914
                     /label=BBa_B0012
     terminator      1874..1914
                     /label=BBa_B0012
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag
      121 aggagaaata ctagatgaat acacaattga tgggtgagcg tattcgcgct cgaagaaaaa
      181 aactcaagat tagacaagcc gctcttggta agatggtggg agtgtctaat gttgcaatat
      241 cgcaatggga gcgctcggag actgagccaa atggggagaa cctgttggca ctttcgaagg
      301 ctcttcagtg ctcccctgac tatttgctga aaggagattt aagccagaca aacgttgcct
      361 atcatagtag gcatgagcca agaggatcat accctcttat cagttgggta agcgcagggc
      421 aatggatgga agctgtagaa ccttatcaca agcgcgcgat agagaactgg cacgacacca
      481 ctgtagattg ttcagaagat tcattttggc ttgatgtcca aggtgactct atgacagcac
      541 cggcagggtt aagcattcca gaaggaatga taattctggt tgatcccgaa gtcgaaccaa
      601 gaaacggcaa gctggttgtt gcaaaattag aaggtgaaaa cgaggccaca ttcaaaaaat
      661 tagttatgga tgcaggccga aagtttttaa aaccattaaa cccacaatat ccgatgatag
      721 aaatcaacgg aaactgcaaa atcattggcg tagttgttga cgcaaaactc gcaaatcttc
      781 cagctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag tgctagtgta
      841 gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      901 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      961 gggtgggcct ttctgcgttt atatactaga gaataaactt gactaaagat tcctttagta
     1021 gataatttaa gtgttcttta atttctacta gagaaagagg agaaatacta gatggcttcc
     1081 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac
     1141 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc
     1201 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg
     1261 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg
     1321 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt
     1381 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa
     1441 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg
     1501 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg
     1561 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct
     1621 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
     1681 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
     1741 ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa
     1801 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1861 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//