LOCUS BBa_M30012 977 bp DNA linear 22-Dec-2024 DEFINITION OmpR controlled mRFP ACCESSION BBa_M30012 VERSION BBa_M30012.1 FEATURES Location/Qualifiers misc_feature 13..30 /label=C1 OmpR misc_feature 34..51 /label=C2 OmpR misc_feature 54..71 /label=C3 OmpR promoter 75..80 /label=-35 promoter 98..103 /label=-10 promoter 1..108 /label=BBa_R0082 misc_feature 121..124 /label=conserved RBS 117..128 /label=BBa_B0034 CDS 135..809 /label=mrfp1 misc_feature 816..840 /label=Help:Barcodes CDS 135..815 /label=BBa_E1010 stem_loop 860..903 /label=stem_loop misc_feature 849..928 /label=BBa_B0010 terminator 849..928 /label=BBa_B0010 stem_loop 944..963 /label=T7 TE misc_feature 970..970 /label=stop polyA_site 964..977 /label=polya misc_feature 937..977 /label=BBa_B0012 terminator 937..977 /label=BBa_B0012 ORIGIN 1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta 61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag 121 aggagaaata ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag 181 ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc 241 gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt 301 tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc 361 cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg 421 ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg 481 gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta 541 tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg 601 ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg 661 aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg 721 acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac 781 gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc 841 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 901 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 961 gcctttctgc gtttata //