LOCUS BBa_M30109 4333 bp DNA linear 22-Dec-2024 DEFINITION Light responsive system, dual regulation ACCESSION BBa_M30109 VERSION BBa_M30109.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 misc_feature 883..907 /label=Help:Barcodes CDS 157..882 /label=BBa_I15008 misc_feature 920..923 /label=conserved RBS 916..927 /label=BBa_B0034 CDS 934..1683 /label=PcyA misc_feature 1684..1708 /label=Help:Barcodes CDS 934..1683 /label=BBa_I15009 stem_loop 1728..1771 /label=stem_loop misc_feature 1717..1796 /label=BBa_B0010 terminator 1717..1796 /label=BBa_B0010 stem_loop 1812..1831 /label=T7 TE misc_feature 1838..1838 /label=stop polyA_site 1832..1845 /label=polya misc_feature 1805..1845 /label=BBa_B0012 terminator 1805..1845 /label=BBa_B0012 misc_feature 1854..1872 /label=TetR 1 misc_feature 1854..1907 /label=BBa_R0040 promoter 1873..1878 /label=-35 misc_feature 1879..1897 /label=TetR 2 promoter 1896..1901 /label=-10 promoter 1854..1907 /label=BBa_R0040 misc_feature 1920..1923 /label=conserved RBS 1916..1927 /label=BBa_B0034 CDS 1934..4171 /label=Cph8 misc_feature 4172..4196 /label=Help:Barcodes CDS 1934..4171 /label=BBa_I15010 stem_loop 4216..4259 /label=stem_loop misc_feature 4205..4284 /label=BBa_B0010 terminator 4205..4284 /label=BBa_B0010 stem_loop 4300..4319 /label=T7 TE misc_feature 4326..4326 /label=stop polyA_site 4320..4333 /label=polya misc_feature 4293..4333 /label=BBa_B0012 terminator 4293..4333 /label=BBa_B0012 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatga gtgtcaactt agcttcccag 181 ttgcgggaag ggacgaaaaa atcccactcc atggcggaga acgtcggctt tgtcaaatgc 241 ttcctcaagg gcgttgtcga gaaaaattcc taccgtaagc tggttggcaa tctctacttt 301 gtctacagtg ccatggaaga ggaaatggca aaatttaagg accatcccat cctcagccac 361 atttacttcc ccgaactcaa ccgcaaacaa agcctagagc aagacctgca attctattac 421 ggctccaact ggcggcaaga agtgaaaatt tctgccgctg gccaagccta tgtggaccga 481 gtccggcaag tggccgctac ggcccctgaa ttgttggtgg cccattccta cacccgttac 541 ctgggggatc tttccggcgg tcaaattctc aagaaaattg cccaaaatgc catgaatctc 601 cacgatggtg gcacagcttt ctatgaattt gccgacattg atgacgaaaa ggcttttaaa 661 aatacctacc gtcaagctat gaatgatctg cccattgacc aagccaccgc cgaacggatt 721 gtggatgaag ccaatgacgc ctttgccatg aacatgaaaa tgttcaacga acttgaaggc 781 aacctgatca aggcgatcgg cattatggtg ttcaacagcc tcacccgtcg ccgcagtcaa 841 ggcagcaccg aagttggcct cgccacctcc gaaggctaat aacgctgata gtgctagtgt 901 agatcgctac tagagaaaga ggagaaatac tagatggccg tcactgattt aagtttgacc 961 aattcttccc tgatgcctac gttgaacccg atgattcaac agttggccct ggcgatcgcc 1021 gctagttggc aaagtttacc cctcaagccc tatcaattgc cggaggattt gggctacgta 1081 gaaggccgcc tggaagggga aaagttagtg attgaaaatc ggtgctacca aacgccccag 1141 tttcgcaaaa tgcatttgga gttggccaag gtgggcaaag ggttggatat tctccactgt 1201 gtaatgtttc ctgagccttt atacggtcta cctttgtttg gctgtgacat tgtggccggc 1261 cccggtggag taagtgcggc tattgcggat ctatccccca cccaaagcga tcgccaattg 1321 cccgcagcgt accaaaaatc attggcagag ctaggccagc cagaatttga gcaacaacgg 1381 gaattgcccc cctggggaga aatattttct gaatattgtt tattcatccg tcccagcaat 1441 gtcactgaag aagaaagatt tgtacaaagg gtagtggact ttttgcaaat tcattgtcac 1501 caatccatcg ttgccgaacc cttgtctgaa gctcaaactt tggagcaccg tcaggggcaa 1561 attcattact gccaacaaca acagaaaaat gataaaaccc gtcgggtact ggaaaaagct 1621 tttggggaag cttgggcgga acggtatatg agccaagtct tatttgatgt tatccaataa 1681 taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg 1741 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1801 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtccctat 1861 cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac tagagaaaga 1921 ggagaaatac tagatggcca ccaccgtaca actcagcgac caatccctcc gtcagctaga 1981 aaccctcgcc atccacaccg cccacctgat tcagccccac ggtttagtgg tggtcctgca 2041 agaaccagac ctcaccatca gccaaattag cgccaactgc accggcattt tagggcgatc 2101 gccagaggat ttgttgggca gaaccctagg ggaagtgttt gatagctttc agattgatcc 2161 catccagagt cgcctaacgg ccggacaaat cagcagcctc aaccccagta aactttgggc 2221 gcgggtcatg ggggacgact ttgtcatttt tgacggggtt tttcatcgca acagtgacgg 2281 tttattggta tgtgaactcg agccagccta cacttccgat aatctgccct tcctcggttt 2341 ttatcacatg gccaacgctg ccctgaatcg gttgcgccaa caagctaatc tacgggattt 2401 ctacgatgtt attgtcgaag aagtccgccg tatgactggc tttgaccggg tgatgctata 2461 ccgctttgat gaaaataacc acggtgatgt cattgccgaa gataaacggg atgatatgga 2521 accctatttg ggcctgcact atcccgaatc ggatattccc caacccgccc gtcggctatt 2581 tatccacaac cccattcgag taattcccga tgtttatggt gtggcggtgc ccctgacccc 2641 agcggttaac cccagcacca accgagcggt ggatttaaca gaatccattc tgcgcagtgc 2701 gtaccattgc cacttgacct atctgaaaaa tatgggggta ggagcgtctt taaccatttc 2761 cctaattaag gacggccatc tctgggggct cattgcctgc caccatcaaa cccccaaagt 2821 aattcccttt gaactgcgta aagcctgcga attttttggt cgggtggtgt ttagcaacat 2881 ttccgcccag gaagatacgg aaaccttcga ttaccgggtg cagctggcgg agcatgaagc 2941 ggttttattg gacaaaatga ccacggcggc ggattttgtc gaaggattaa ctaatcatcc 3001 cgatcgcctg ttgggattaa cgggctccca gggggcggcc atttgctttg gggaaaaatt 3061 gattttagta ggggaaaccc cggacgagaa agcagtgcaa tatttactgc aatggttgga 3121 gaatcgggaa gtgcaagacg ttttcttcac ctcttccctc tcacaaattt atcctgatgc 3181 agtgaatttt aaatccgtgg ccagtggctt attggccatt cccattgccc gtcacaactt 3241 tttgctctgg tttcgccctg aagtgttgca aacggttaat tggggcggtg acccaaatca 3301 tgcttacgaa gctacccagg aagacggtaa aatcgagctc catccccgcc aatcctttga 3361 cctctggaaa gaaattgtcc gactccaatc tttgccctgg caatcggtgg aaatccaaag 3421 tgccctggcc ctgaaaaagg cgatcgtcaa cctcattttg cgccaggcag aagaattgca 3481 tatggcggct ggtgttaagc aactggcgga tgaccgcacg ctgctgatgg cgggggtaag 3541 tcacgacttg cgcacgccgc tgacgcgtat tcgcctggcg actgagatga tgagcgagca 3601 ggatggctat ctggcagaat cgatcaataa agatatcgaa gagtgcaacg ccatcattga 3661 gcagtttatc gactacctgc gcaccgggca ggagatgccg atggaaatgg cggatcttaa 3721 tgcagtactc ggtgaggtga ttgctgccga aagtggctat gagcgggaaa ttgaaaccgc 3781 gctttacccc ggcagcattg aagtgaaaat gcacccgctg tcgatcaaac gcgcggtggc 3841 gaatatggtg gtcaacgccg cccgttatgg caatggctgg gtcaaagtca gcagcggaac 3901 ggagccgaat cgcgcctggt tccaggtgga agatgacggt ccgggaattg cgccggaaca 3961 acgtaagcac ctgttccagc cgtttgtccg cggcgacagt gcgcgcacca ttagcggcac 4021 gggattaggg ctggcaattg tgcagcgtat cgtggataac cataacggga tgctggagct 4081 tggcaccagc gagcggggcg ggctttccat tcgcgcctgg ctgccagtgc cggtaacgcg 4141 ggcgcagggc atgacaaaag aagggtaata actctgatag tgctagtgta gatctctact 4201 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 4261 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 4321 ttctgcgttt ata //