LOCUS       BBa_M30333              2903 bp    DNA     linear       27-Sep-2024
DEFINITION  Refactored M13 Between HpaI and BamHI Sites
ACCESSION   BBa_M30333
VERSION     BBa_M30333.1  
FEATURES             Location/Qualifiers
     CDS             1..831
                     /label=g2 (incomplete)
     misc_feature    1..3
                     /label=HpaI
     promoter        381..428
                     /label=g10-promoter (disrupted)
     RBS             480..495
                     /label=g10-RBS (disrupted)
     misc_feature    1..831
                     /label=BBa_M31112
     misc_feature    840..845
                     /label=SphI
     CDS             910..1245
                     /label=g10
     promoter        846..893
                     /label=g10-promoter
     RBS             894..909
                     /label=g10-RBS
     misc_feature    1246..1251
                     /label=SphI
     misc_feature    840..1251
                     /label=BBa_M31010
     misc_feature    1260..1265
                     /label=XbaI
     misc_feature    1587..1592
                     /label=XbaI
     promoter        1266..1315
                     /label=g5-promoter
     RBS             1307..1322
                     /label=g5_RBS
     CDS             1323..1586
                     /label=g5
     misc_feature    1260..1592
                     /label=BBa_M31005
     misc_feature    1601..1606
                     /label=SpeI
     misc_feature    1725..1730
                     /label=SpeI
     promoter        1607..1622
                     /label=g7-promoter
     CDS             1623..1724
                     /label=g7
     misc_feature    1601..1730
                     /label=BBa_M31007
     misc_feature    1739..1744
                     /label=NcoI
     misc_feature    1860..1865
                     /label=NcoI
     promoter        1745..1760
                     /label=g9-promoter
     CDS             1761..1859
                     /label=g9
     misc_feature    1739..1865
                     /label=BBa_M31979
     misc_feature    1874..1879
                     /label=SalI
     misc_feature    2164..2169
                     /label=SalI
     promoter        1880..1925
                     /label=g8-promoter
     RBS             1926..1941
                     /label=g8-RBS
     CDS             1942..2163
                     /label=g8
     misc_feature    1874..2169
                     /label=BBa_M31998
     promoter        2178..2225
                     /label=g3-promoter
     misc_feature    2898..2903
                     /label=BamHI
     RBS             2241..2256
                     /label=g3-RBS
     CDS             2257..2903
                     /label=g3
     misc_feature    2178..2903
                     /label=BBa_M31999
ORIGIN
        1 aacgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
       61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
      121 cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
      181 gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
      241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
      301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
      361 tctttcgggc ttcctcttaa tctgttcgac gccattcgtt tcgcatctga ttacaacagc
      421 caaggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
      481 ttcgaaggtg actctacgaa tatttatgac gattccgcag tattggacgc tatccagtct
      541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
      601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
      661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
      721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
      781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata atactagagg
      841 catgctcttt ttgatgcaat ccgctttgct tctgactata atagtcaggg taaatttgag
      901 ggggattcaa tgaatattta tgacgattcc gcagtattgg acgctatcca gtctaaacat
      961 tttactatta ccccctctgg caaaacttct tttgcaaaag cctctcgcta ttttggtttt
     1021 tatcgtcgtc tggtaaacga gggttatgat agtgttgctc ttactatgcc tcgtaattcc
     1081 ttttggcgtt atgtatctgc attagttgaa tgtggtattc ctaaatctca actgatgaat
     1141 ctttctacct gtaataatgt tgttccgtta gttcgtttta ttaacgtaga tttttcttcc
     1201 caacgtcctg actggtataa tgagccagtt cttaaaatcg cataagcatg ctactagagt
     1261 ctagaccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
     1321 caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
     1381 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
     1441 aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
     1501 tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
     1561 gtctgcgcct cgttccggct aagtaatcta gatactagag actagtgttc cggctaagta
     1621 acatggagca ggtcgcggat ttcgacacaa tttatcaggc gatgatacaa atctccgttg
     1681 tactttgttt cgcgcttggt ataatcgctg ggggtcaaag gtaaactagt tactagagcc
     1741 atggtcgctg ggggtcaaag atgagtgttt tagtgtattc tttcgcctct ttcgttttag
     1801 gttggtgcct tcgtagtggc attacgtatt ttacccgttt aatggaaact tcctcgtaac
     1861 catggtacta gaggtcgaca tctccgttgt actttgtttc gcgcttggta taatcgctgg
     1921 gggtctaatg gaaacttcct catgaaaaag tctttagtcc tcaaagcctc tgtagccgtt
     1981 gctaccctcg ttccgatgct gtctttcgct gctgagggtg acgatcccgc aaaagcggcc
     2041 tttaactccc tgcaagcctc agcgaccgaa tatatcggtt atgcgtgggc gatggttgtt
     2101 gtcattgtcg gcgcaactat cggtatcaag ctgtttaaga aattcacctc gaaagcaagc
     2161 tgagtcgact actagagaat tcacctcgaa agcaagctga taaaccgata caattaaagg
     2221 ctccttttgg agcctttttt tttggagatt ttcaacgtga aaaaattatt attcgcaatt
     2281 cctttagttg ttcctttcta ttctcactcc gctgaaactg ttgaaagttg tttagcaaaa
     2341 ccccatacag aaaattcatt tactaacgtc tggaaagacg acaaaacttt agatcgttac
     2401 gctaactatg agggttgtct gtggaatgct acaggcgttg tagtttgtac tggtgacgaa
     2461 actcagtgtt acggtacatg ggttcctatt gggcttgcta tccctgaaaa tgagggtggt
     2521 ggctctgagg gtggcggttc tgagggtggc ggttctgagg gtggcggtac taaacctcct
     2581 gagtacggtg atacacctat tccgggctat acttatatca accctctcga cggcacttat
     2641 ccgcctggta ctgagcaaaa ccccgctaat cctaatcctt ctcttgagga gtctcagcct
     2701 cttaatactt tcatgtttca gaataatagg ttccgaaata ggcagggggc attaactgtt
     2761 tatacgggca ctgttactca aggcactgac cccgttaaaa cttattacca gtacactcct
     2821 gtatcatcaa aagccatgta tgacgcttac tggaacggta aattcagaga ctgcgctttc
     2881 cattctggct ttaatgagga tcc
//