LOCUS       BBa_M31002              1233 bp    DNA     linear       16-Feb-2025
DEFINITION  Refactored Gene 2 without Gene 10's Promoter or RBS
ACCESSION   BBa_M31002
VERSION     BBa_M31002.1  
FEATURES             Location/Qualifiers
     misc_feature    400..406
                     /label=Hpal1
     promoter        783..830
                     /label=disrupted gene 10 promoter
     misc_feature    786..789
                     /label=t to c (786) and t to c (789)
     RBS             882..897
                     /label=disrupted gene 10 Rbs
     misc_feature    885..885
                     /label=t to c (885)
ORIGIN
        1 atgattgaca tgctagtttt acgattaccg ttcatcgatt ctcttgtttg ctccagactc
       61 tcaggcaatg acctgatagc ctttgtagac ctctcaaaaa tagctaccct ctccggcatg
      121 aatttatcag ctagaacggt tgaatatcat attgatggtg atttgactgt ctccggcctt
      181 tctcaccctt ttgaatcttt acctacacat tactcaggca ttgcatttaa aatatatgag
      241 ggttctaaaa atttttatcc ttgcgttgaa ataaaggctt ctcccgcaaa agtattacag
      301 ggtcataatg tttttggtac aaccgattta gctttatgct ctgaggcttt attgcttaat
      361 tttgctaatt ctttgccttg cctgtatgat ttattggatg ttaacgctac tactattagt
      421 agaattgatg ccaccttttc agctcgcgcc ccaaatgaaa atatagctaa acaggttatt
      481 gaccatttgc gaaatgtatc taatggtcaa actaaatcta ctcgttcgca gaattgggaa
      541 tcaactgtta catggaatga aacttccaga caccgtactt tagttgcata tttaaaacat
      601 gttgagctac agcaccagat tcagcaatta agctctaagc catccgcaaa aatgacctct
      661 tatcaaaagg agcaattaaa ggtactctct aatcctgacc tgttggagtt tgcttccggt
      721 ctggttcgct ttgaagctcg aattaaaacg cgatatttga agtctttcgg gcttcctctt
      781 aatctcttcg atgcaatccg ctttgcttct gactataata gtcagggtaa agacctgatt
      841 tttgatttat ggtcattctc gttttctgaa ctgtttaaag cattcgaggg ggattcaatg
      901 aatatttatg acgattccgc agtattggac gctatccagt ctaaacattt tactattacc
      961 ccctctggca aaacttcttt tgcaaaagcc tctcgctatt ttggttttta tcgtcgtctg
     1021 gtaaacgagg gttatgatag tgttgctctt actatgcctc gtaattcctt ttggcgttat
     1081 gtatctgcat tagttgaatg tggtattcct aaatctcaac tgatgaatct ttctacctgt
     1141 aataatgttg ttccgttagt tcgttttatt aacgtagatt tttcttccca acgtcctgac
     1201 tggtataatg agccagttct taaaatcgca taa
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