LOCUS       BBa_M31173              2330 bp    DNA     linear       16-Feb-2025
DEFINITION  M13.1 from HpaI to BamHI, for reals this time!
ACCESSION   BBa_M31173
VERSION     BBa_M31173.1  
FEATURES             Location/Qualifiers
     CDS             1..831
                     /label=g2 ORF (incomplete)
     misc_feature    1..3
                     /label=HpaI (incomplete)
     promoter        381..428
                     /label=g10 promoter
     RBS             480..495
                     /label=g10 RBS
     CDS             496..831
                     /label=g10 ORF
     promoter        785..835
                     /label=g5 promoter
     RBS             827..842
                     /label=g5 RBS
     CDS             843..1106
                     /label=g5 ORF
     RBS             1092..1107
                     /label=g7 RBS
     CDS             1108..1209
                     /label=g7 ORF
     misc_feature    1210..1215
                     /label=MfeI
     RBS             1216..1231
                     /label=g9 RBS
     CDS             1232..1330
                     /label=g9 ORF
     misc_feature    1331..1336
                     /label=NcoI
     promoter        1337..1383
                     /label=g8 promoter
     RBS             1384..1399
                     /label=g8 RBS
     CDS             1400..1621
                     /label=g8 ORF
     misc_feature    1647..1652
                     /label=NheI
     promoter        1599..1646
                     /label=g3 promoter
     RBS             1668..1683
                     /label=g3 RBS
     CDS             1684..2330
                     /label=g3 ORF (incomplete)
     misc_feature    2325..2330
                     /label=BamHI
ORIGIN
        1 aacgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
       61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
      121 cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
      181 gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
      241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
      301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
      361 tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
      421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
      481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
      541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
      601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
      661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
      721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
      781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
      841 caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
      901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
      961 aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
     1021 tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
     1081 gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
     1141 caggcgatga tacagatatc ggtagttcta tgctttgccc taggaattat tgctggggga
     1201 caaagatgac aattgtcgct gggggtcaaa gatgagtgtt ttagtgtatt ctttcgcctc
     1261 tttcgtttta ggttggtgcc ttcgtagtgg cattacgtat tttacccgtt tgatggagac
     1321 ttcatcatga ccatggaatc tccgttgtac tttgtttcgc gcttggtata atcgctgggg
     1381 gtctaatgga aacttcctca tgaaaaagtc tttagtcctc aaagcctctg tagccgttgc
     1441 taccctcgtt ccgatgctgt ctttcgctgc tgagggtgac gatcccgcaa aagcggcctt
     1501 taactccctg caagcctcag cgaccgaata tatcggttat gcgtgggcga tggttgttgt
     1561 cattgtcggc gcaactatcg gtatcaagct gtttaagaaa ttcacctcga aagcaagctg
     1621 ataaaccgat acaattaaag gctcctgcta gctttggagc cttttttttt ggagattttc
     1681 aacgtgaaaa aattattatt cgcaattcct ttagttgttc ctttctattc tcactccgct
     1741 gaaactgttg aaagttgttt agcaaaaccc catacagaaa attcatttac taacgtctgg
     1801 aaagacgaca aaactttaga tcgttacgct aactatgagg gttgtctgtg gaatgctaca
     1861 ggcgttgtag tttgtactgg tgacgaaact cagtgttacg gtacatgggt tcctattggg
     1921 cttgctatcc ctgaaaatga gggtggtggc tctgagggtg gcggttctga gggtggcggt
     1981 tctgagggtg gcggtactaa acctcctgag tacggtgata cacctattcc gggctatact
     2041 tatatcaacc ctctcgacgg cacttatccg cctggtactg agcaaaaccc cgctaatcct
     2101 aatccttctc ttgaggagtc tcagcctctt aatactttca tgtttcagaa taataggttc
     2161 cgaaataggc agggggcatt aactgtttat acgggcactg ttactcaagg cactgacccc
     2221 gttaaaactt attaccagta cactcctgta tcatcaaaag ccatgtatga cgcttactgg
     2281 aacggtaaat tcagagactg cgctttccat tctggcttta atgaggatcc
//