BBa_M31234 1 BBa_M31234 Calcium channel-apoaequorin fusion 2008-05-12T11:00:00Z 2015-05-08T01:13:59Z Accession number for Homo sapiens neuronal voltage gated calcium channel gamma-3 subunit = AF100346 Accession number for Aequorea victoria apoaequorin = E14214 This part encodes for the homo sapiens neuronal voltage-gated calcium ion channel gamma-3 subunit linked to Aequorea victoria apoaequorin. The ion channel is expressed in the membrane of the presynaptic cleft, with an apoaequorin linked to the C-terminal of the channel. If coelenterazine is present within the neuron, it binds to the apoaequorin along with oxygen. When an action potential reaches the modified ion channel, calcium ions flood in to the cell, and 2 or 3 of them bind to the apoaequorin. When the calcium ions bind, the coelenterazine is oxidized, and light in the form of bioluminescence is emitted as a byproduct. This part, when used in tandem with coelenterazine will show the activity of neurons by bioluminescing whenever an action potential reaches the synapse. false false _102_ 0 1145 102 Not in stock false Designing an appropriate linker false Kate Broadbent annotation1963085 1 linker range1963085 1 946 984 annotation1963087 1 stop range1963087 1 1843 1845 annotation1963086 1 apoaequorin range1963086 1 985 988 annotation1963084 1 calcium channel range1963084 1 1 3 BBa_M31234_sequence 1 atgaggatgtgtgacagaggtatccagatgttgatcaccactgtaggagcctttgccgcttttagtttaatgaccattgcagtgggcacggactactggttatattccagaggtgtgtgcaggactaaatctacaagtgataatgaaaccagcaggaagaatgaagaagtaatgacccattcggggctgtggaggacctgctgcctagaaggggctttccgaggcgtgtgcaagaaaatcgatcacttccctgaagatgctgactacgaacaggacacagccgaatatctcctgcgagctgtgagggcctccagtgtcttccccatcctcagtgtcacgctgctgttcttcggcgggctctgcgtggcagccagtgagttccaccgcagcagacacaacgtcattctcagcgcgggcatcttttttgtctctgcagggttaagcaacatcattggcatcatagtttatatatcagccaacgccggagaccccgggcagcgtgactccaaaaaaagttactcctatggttggtccttttatttcggagccttctctttcatcatcgcagaaattgtaggagtggttgccgtgcacatctatattgaaaaacatcagcagttacgagccaaatcccactcggagttcctgaagaaatctacttttgcccgcctcccaccctacaggtatcgattccggaggcggtcaagttctcgctccaccgagcccagatcccgagacctgtcccccatcagcaaaggcttccacaccatcccttccactgacatctcgatgttcaccctctcccgggacccctcaaagatcaccatggggaccctcctcaactccgaccgggaccacgcttttctacagttccacaattccacacccaaagagttcaaagagtcactgcataataatccggccaacaggcgcaccacgcccgtcattcggtccggcgggagcggatccggcggccagtccccgaatgcaattcatctttgcatcaaagaattacatcaaatctctagttgatcaactaaattgtctcgacaacaacaagcaaacatgacaagcaaacaatactcagtcaagcttacatcagacttcgacaacccaagatggattggacgacacaagcatatgttcaatttccttgatgtcaaccacaatggaaaaatctctcttgacgagatggtctacaaggcatctgatattgtcatcaataaccttggagcaacacctgagcaagccaaacgacacaaagatgctgtagaagccttcttcggaggagctggaatgaaatatggtgtggaaactgattggcctgcatatattgaaggatggaaaaaattggctactgatgaattggagaaatacgccaaaaacgaaccaacgctcatccgtatatggggtgatgctttgtttgatatcgttgacaaagatcaaaatggagccattacactggatgaatggaaagcatacaccaaagctgctggtatcatccaatcatcagaagattgcgaggaaacattcagagtgtgcgatattgatgaaagtggacaactcgatgttgatgagatgacaagacaacatttaggattttggtacaccatggatcctgcttgcgaaaagctctacggtggagctgtcccctaagaagctctacggtggtgatgcaccctgggaagatgatgtgattttgaataaaacactgatgaattcaatcaaaattttccaaatttttgaacgatttcaatcgtttgtgttgatttttgtaattaggaacagattaaatcgaatgattagttgtttttttaatcaacagaacttacaaatcgaaaaagt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z