LOCUS       BBa_M31415              2874 bp    DNA     linear       23-Dec-2024
DEFINITION  20.109 refactor
ACCESSION   BBa_M31415
VERSION     BBa_M31415.1  
FEATURES             Location/Qualifiers
     CDS             1..834
                     /label=mod g2
     promoter        841..888
                     /label=g10 promoter
     RBS             895..910
                     /label=g10 RBS
     CDS             911..1246
                     /label=mod g10
     promoter        1253..1302
                     /label=g5 promoter
     RBS             1311..1326
                     /label=g5 RBS
     CDS             1327..1609
                     /label=mod g5
     misc_feature    1524..1529
                     /label=BsrGI
     RBS             1616..1631
                     /label=g7 RBS
     CDS             1632..1733
                     /label=mod g7
     promoter        1740..1786
                     /label=g8 promoter
     RBS             1793..1808
                     /label=g9 RBS
     CDS             1809..1907
                     /label=mod g9
     misc_feature    1871..1876
                     /label=SnaBI
     RBS             1914..1929
                     /label=g8 RBS
     CDS             1930..2151
                     /label=mod g8
     promoter        2158..2205
                     /label=g3 promoter
     misc_feature    1..6
                     /label=HpaI
     RBS             2212..2227
                     /label=g3 RBS
     CDS             2228..2874
                     /label=mod g3
     misc_feature    2869..2874
                     /label=BamHI
     misc_feature    835..840
                     /label=AatII
     misc_feature    889..894
                     /label=Acc65I
     misc_feature    1247..1252
                     /label=ApaI
     misc_feature    1303..1310
                     /label=AscI
     misc_feature    1610..1615
                     /label=BclI
     misc_feature    1734..1739
                     /label=BmtI
     misc_feature    1787..1792
                     /label=BsiWI
     misc_feature    1908..1913
                     /label=EcoRI
     misc_feature    2152..2157
                     /label=KasI
     misc_feature    2206..2211
                     /label=MfeI
ORIGIN
        1 gttaacgcta ctactattag tagaattgat gccacctttt cagctcgcgc cccaaatgaa
       61 aatatagcta aacaggttat tgaccatttg cgaaatgtat ctaatggtca aactaaatct
      121 actcgttcgc agaattggga atcaactgtt acatggaatg aaacttccag acaccgtact
      181 ttagttgcat atttaaaaca tgttgagcta cagcaccaga ttcagcaatt aagctctaag
      241 ccatccgcaa aaatgacctc ttatcaaaag gagcaattaa aggtactctc taatcctgac
      301 ctgttggagt ttgcttccgg tctggttcgc tttgaagctc gaattaaaac gcgatatttg
      361 aagtctttcg ggcttcctct taacctattc gacgctatac ggttcgcatc agattacaac
      421 agccaaggaa aagacctgat ttttgattta tggtcattct cgttttctga actgtttaaa
      481 gctttcgaag gcgactcttg taacatatac gatgactcgg ctgtttttga tgcaatacaa
      541 tcaaagcact tcacaataac gccgtcaggg aagacatcat tcgctaaggc gtcacggtac
      601 ttcggattct accgacgact cgttaatgaa ggatacgaca gcgtagcact aacaatgcca
      661 cgaaactcgt tctggcgata cgtttcagct ttggtagagt gcggaatacc aaagtcacag
      721 ctcatgaacc tatcaacgtg caacaacgta gtacccttgg tacgattcat aaatgttgac
      781 ttctcatcgc agcgaccaga ttggtacaac gaacctgtac taaagatagc ttaggacgtc
      841 tctttttgat gcaatccgct ttgcttctga ctataatagt cagggtaagg taccatttga
      901 gggggattca atgaatattt atgacgattc cgcagtattg gacgctatcc agtctaaaca
      961 ttttactatt accccctctg gcaaaacttc ttttgcaaaa gcctctcgct attttggttt
     1021 ttatcgtcgt ctggtaaacg agggttatga tagtgttgct cttactatgc ctcgtaattc
     1081 cttttggcgt tatgtatctg cattagttga atgtggtatt cctaaatctc aactgatgaa
     1141 tctttctacc tgtaataatg ttgttccgtt agttcgtttt attaacgtag atttttcttc
     1201 acagcggccg gattggtaca acgaaccggt gctgaagatt gcctaggggc ccccaacgtc
     1261 ctgactggta taatgagcca gttcttaaaa tcgcataagg taggcgcgcc cataaggtaa
     1321 ttcacaatga ttaaggtaat tcacaatgat taaagttgaa attaaaccat ctcaagccca
     1381 atttactact cgttctggtg tttctcgtca gggcaagcct tattcactga atgagcagct
     1441 ttgttacgtt gatttgggta atgaatatcc ggttcttgtc aagattactc ttgatgaagg
     1501 tcagccagcc tatgcgcctg gtctgtacac cgttcatctg tcctctttca aagttggtca
     1561 gttcggttcc cttatgattg accgtctgcg cctcgtaccc gcaaaatagt gatcagttcc
     1621 ggctaagtaa catggagcag gtcgcggatt tcgacacaat ttatcaggcg atgatacaga
     1681 tatcggtagt tctatgcttt gcactaggaa ttatagcagg cggacagagg taggctagca
     1741 atctccgttg tactttgttt cgcgcttggt ataatcgctg ggggtccgta cgtcgctggg
     1801 ggtcaaagat gagtgtttta gtgtattctt tcgcctcttt cgttttaggt tggtgccttc
     1861 gtagtggcat tacgtatttt acccgtttga tggagacatc gtcttaggaa ttctaatgga
     1921 aacttcctca tgaaaaagtc tttagtcctc aaagcctctg tagccgttgc taccctcgtt
     1981 ccgatgctgt ctttcgctgc tgagggtgac gatcccgcaa aagcggcctt taactccctg
     2041 caagcctcag cgaccgaata tatcggttat gcgtgggcga tggttgttgt cattgtcggc
     2101 gcaactatcg gtatcaagct gtttaagaag tttacgtcca aggctagtta gggcgccaat
     2161 tcacctcgaa agcaagctga taaaccgata caattaaagg ctcctcaatt gtttggagat
     2221 tttcaacgtg aaaaaattat tattcgcaat tcctttagtt gttcctttct attctcactc
     2281 cgctgaaact gttgaaagtt gtttagcaaa accccataca gaaaattcat ttactaacgt
     2341 ctggaaagac gacaaaactt tagatcgtta cgctaactat gagggttgtc tgtggaatgc
     2401 tacaggcgtt gtagtttgta ctggtgacga aactcagtgt tacggtacat gggttcctat
     2461 tgggcttgct atccctgaaa atgagggtgg tggctctgag ggtggcggtt ctgagggtgg
     2521 cggttctgag ggtggcggta ctaaacctcc tgagtacggt gatacaccta ttccgggcta
     2581 tacttatatc aaccctctcg acggcactta tccgcctggt actgagcaaa accccgctaa
     2641 tcctaatcct tctcttgagg agtctcagcc tcttaatact ttcatgtttc agaataatag
     2701 gttccgaaat aggcaggggg cattaactgt ttatacgggc actgttactc aaggcactga
     2761 ccccgttaaa acttattacc agtacactcc tgtatcatca aaagccatgt atgacgctta
     2821 ctggaacggt aaattcagag actgcgcttt ccattctggc tttaatgagg atcc
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