LOCUS       BBa_M31512              2779 bp    DNA     linear       12-May-2024
DEFINITION  section of M13.1 (HpaI to BamHI section)
ACCESSION   BBa_M31512
VERSION     BBa_M31512.1  
FEATURES             Location/Qualifiers
     CDS             496..831
                     /label=gene 10 ORF
     CDS             1..831
                     /label=gene 2 ORF (inc)
     misc_feature    1..3
                     /label=HpaI (inc)
     CDS             900..1163
                     /label=gene 5 ORF
     CDS             1191..1292
                     /label=gene 7 ORF
     CDS             1355..1453
                     /label=gene 9 ORF
     CDS             1481..1702
                     /label=gene 8.1 ORF
     misc_feature    1368..1373
                     /label=PstI
     CDS             2133..2779
                     /label=gene 3 ORF (inc)
     misc_feature    2774..2779
                     /label=BamHI
     promoter        843..892
                     /label=promoter g5
     promoter        381..428
                     /label=promoter g10
     RBS             480..495
                     /label=rbs g10
     RBS             893..899
                     /label=rbs g5
     misc_feature    1078..1083
                     /label=BsrGI
     RBS             1175..1190
                     /label=rbs g7
     promoter        1304..1350
                     /label=promoter g8.1
     RBS             1465..1480
                     /label=rbs g8.1
     promoter        2054..2100
                     /label=promoter g3
     RBS             2117..2132
                     /label=rbs g3
     RBS             1339..1354
                     /label=rbs g9
     misc_feature    1268..1273
                     /label=SnaBI
     promoter        1747..1793
                     /label=gene 8.2 promoter
     RBS             1794..1809
                     /label=rbs g8.2
     CDS             1810..2031
                     /label=gene 8.2 ORF
     misc_feature    832..837
                     /label=KpnI
     misc_feature    837..842
                     /label=EagI
     misc_feature    1164..1169
                     /label=ApaI
     misc_feature    1169..1174
                     /label=AvrII
     misc_feature    1293..1298
                     /label=BspEI
     misc_feature    1298..1303
                     /label=BglII
     misc_feature    1454..1459
                     /label=NcoI
     misc_feature    1459..1464
                     /label=NarI
     misc_feature    1703..1708
                     /label=SpeI
     misc_feature    1708..1713
                     /label=BclI
     misc_feature    1714..1719
                     /label=KasI
     misc_feature    1719..1724
                     /label=BsiWI
     misc_feature    2032..2037
                     /label=SpeI
     misc_feature    2037..2042
                     /label=BclI
     misc_feature    2043..2048
                     /label=KasI
     misc_feature    2048..2053
                     /label=BsiWI
ORIGIN
        1 aacgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
       61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
      121 cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
      181 gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
      241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
      301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
      361 tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
      421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
      481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
      541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
      601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
      661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
      721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
      781 tcttcccaac gaccggattg gtataacgag ccagtgctta agatcgctta gggtaccggc
      841 cgccaacgtc ctgactggta taatgagcca gttcttaaaa tcgcataagg taattcacaa
      901 tgattaaagt tgaaattaaa ccatctcaag cccaatttac tactcgttct ggtgtttctc
      961 gtcagggcaa gccttattca ctgaatgagc agctttgtta cgttgatttg ggtaatgaat
     1021 atccggttct tgtcaagatt actcttgatg aaggtcagcc agcctatgcg cctggtctgt
     1081 acaccgttca tctgtcctct ttcaaagttg gtcagttcgg ttcccttatg attgaccgtc
     1141 tgcgcctcgt acctgcaaaa taagggcccc tagggttccg gctaagtaac atggagcagg
     1201 tcgcggattt cgacacaatt tatcaggcga tgatacaaat atccgtagtg ctttgcttcg
     1261 ctctagggat aatagctggc gggcaaaggt gatccggaga tctaatctcc gttgtacttt
     1321 gtttcgcgct tggtataatc gctgggggtc aaagatgagt gttttagtgt attctttcgc
     1381 ctctttcgtt ttaggttggt gccttcgtag tggcattacg tattttaccc gtttaatgga
     1441 gacttcgtct tgaccatggg cgcctaatgg aaacttcctc atgaaaaagt ctttagtcct
     1501 caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
     1561 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
     1621 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
     1681 atttacgtcc aaggctagtt gaactagtga tcaggcgccg tacgaattca cctcgaaagc
     1741 aagctgaatc tccgttgtac tttgtttcgc gcttggtata atcgctgggg gtctaatgga
     1801 aacttcctca tgaaaaagtc tttagtcctc aaagcctctg tagccgttgc taccctcgtt
     1861 ccgatgctgt ctttcgctgc tgagggtgac gatcccgcaa aagcggcctt taactccctg
     1921 caagcctcag cgaccgaata tatcggttat gcgtgggcga tggttgttgt cattgtcggc
     1981 gcaactatcg gtatcaagct gtttaagaaa tttacgtcca aggctagttg aactagtgat
     2041 caggcgccgt acgaattcac ctcgaaagca agctgataaa ccgatacaat taaaggctcc
     2101 ttttggagcc tttttttttg gagattttca acgtgaaaaa attattattc gcaattcctt
     2161 tagttgttcc tttctattct cactccgctg aaactgttga aagttgttta gcaaaacccc
     2221 atacagaaaa ttcatttact aacgtctgga aagacgacaa aactttagat cgttacgcta
     2281 actatgaggg ttgtctgtgg aatgctacag gcgttgtagt ttgtactggt gacgaaactc
     2341 agtgttacgg tacatgggtt cctattgggc ttgctatccc tgaaaatgag ggtggtggct
     2401 ctgagggtgg cggttctgag ggtggcggtt ctgagggtgg cggtactaaa cctcctgagt
     2461 acggtgatac acctattccg ggctatactt atatcaaccc tctcgacggc acttatccgc
     2521 ctggtactga gcaaaacccc gctaatccta atccttctct tgaggagtct cagcctctta
     2581 atactttcat gtttcagaat aataggttcc gaaataggca gggggcatta actgtttata
     2641 cgggcactgt tactcaaggc actgaccccg ttaaaactta ttaccagtac actcctgtat
     2701 catcaaaagc catgtatgac gcttactgga acggtaaatt cagagactgc gctttccatt
     2761 ctggctttaa tgaggatcc
//