LOCUS       BBa_M31513              2302 bp    DNA     linear       22-Dec-2024
DEFINITION  Refactored portion of M13 genome from unique Hpal site on gene II 
            to the BamHI site on gene III
ACCESSION   BBa_M31513
VERSION     BBa_M31513.1  
FEATURES             Location/Qualifiers
     promoter        381..428
                     /label=promoter-g10
     RBS             480..495
                     /label=rbs-g10
     promoter        786..835
                     /label=promoter g5
     RBS             827..842
                     /label=rbs-g5
     CDS             843..1106
                     /label=g5 (ORF)
     misc_feature    1021..1025
                     /label=BsrGI
     promoter        1317..1364
                     /label=promoter-g8
     RBS             1190..1205
                     /label=rbs-g9
     CDS             1409..1630
                     /label=gene VIII
     promoter        1577..1624
                     /label=promoter-g3
     RBS             1640..1655
                     /label=rbs-g3
     CDS             1656..2302
                     /label=gene III (ORF) incomplete
     misc_feature    2220..2224
                     /label=BamHI
     CDS             1206..1306
                     /label=gene IX (ORF)
     RBS             1365..1381
                     /label=rbs-g8
     misc_feature    1307..1311
                     /label=BamHI
     misc_feature    1312..1317
                     /label=Eag1
     CDS             496..831
                     /label=gene X (ORF)
     CDS             1..831
                     /label=gene II (ORF) incomplete
ORIGIN
        1 aacgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
       61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
      121 cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
      181 gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
      241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
      301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
      361 tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
      421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
      481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
      541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
      601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
      661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
      721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
      781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
      841 caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
      901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
      961 aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
     1021 tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
     1081 gtctgcgcct cgttccagct aagtaacatg gagcaggtcg cggatttcga cacaatttat
     1141 caggcgatga tacaaacctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
     1201 caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
     1261 gtggcattac gtattttacc cgtttaatgt aaacttcctc acgaaaggat ccggccgaat
     1321 ctccgttgta ctttgtttcg cgcttggtat aatcgctggg ggtctaatgg aaacttcctc
     1381 atgaagtctt tagtcctcaa agcctctgta gccgttgcta ccctcgttcc gatgctgtct
     1441 ttcgctgctg agggtgacga tcccgcaaaa gcggccttta actccctgca agcctcagcg
     1501 accgaatata tcggttatgc gtgggcgatg gttgttgtca ttgtcggcgc aactatcggt
     1561 atcaagctgt ttaagaaatt cacctcgaaa gcaagctgat aaaccgatac aattaaaggc
     1621 tccttttgga gccttttttt ttggagattt tcaacgtgaa aaaattatta ttcgcaattc
     1681 ctttagttgt tcctttctat tctcactccg ctgaaactgt tgaaagttgt ttagcaaaac
     1741 cccatacaga aaattcattt actaacgtct ggaaagacga caaaacttta gatcgttacg
     1801 ctaactatga gggttgtctg tggaatgcta caggcgttgt agtttgtact ggtgacgaaa
     1861 ctcagtgtta cggtacatgg gttcctattg ggcttgctat ccctgaaaat gagggtggtg
     1921 gctctgaggg tggcggttct gagggtggcg gttctgaggg tggcggtact aaacctcctg
     1981 agtacggtga tacacctatt ccgggctata cttatatcaa ccctctcgac ggcacttatc
     2041 cgcctggtac tgagcaaaac cccgctaatc ctaatccttc tcttgaggag tctcagcctc
     2101 ttaatacttt catgtttcag aataataggt tccgaaatag gcagggggca ttaactgttt
     2161 atacgggcac tgttactcaa ggcactgacc ccgttaaaac ttattaccag tacactcctg
     2221 tatcatcaaa agccatgtat gacgcttact ggaacggtaa attcagagac tgcgctttcc
     2281 attctggctt taatgaggat cc
//