BBa_M31513 1 BBa_M31513 Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III 2007-02-26T12:00:00Z 2015-05-08T01:14:00Z The source for this part was the genmoic sequence of the M13 bacteriophage This part has been refactored so that the re-engineering of genes within the part are made easier. We used the methods outlined by researches that refactored the T7 genome,which required inserting 'buffer regions' in between genes that were overlapping or embedded in each other. false false _102_ 0 1382 102 Not in stock false We had to consider the fact that basepairs within the regions that encoded for genes could not be altered too much since we want to maintain functionality of M13 after refactoring this segment of its genome false Amirah Khan annotation1914992 1 gene VIII range1914992 1 1409 1630 annotation1914996 1 BamHI range1914996 1 2220 2224 annotation1915210 1 gene X (ORF) range1915210 1 496 831 annotation1914987 1 rbs-g5 range1914987 1 827 842 annotation1915179 1 Eag1 range1915179 1 1312 1317 annotation1914994 1 rbs-g3 range1914994 1 1640 1655 annotation1915211 1 gene II (ORF) incomplete range1915211 1 1 831 annotation1915002 1 gene IX (ORF) range1915002 1 1206 1306 annotation1914984 1 promoter-g10 range1914984 1 381 428 annotation1914993 1 promoter-g3 range1914993 1 1577 1624 annotation1914986 1 promoter g5 range1914986 1 786 835 annotation1915171 1 rbs-g8 range1915171 1 1365 1381 annotation1914989 1 BsrGI range1914989 1 1021 1025 annotation1914988 1 g5 (ORF) range1914988 1 843 1106 annotation1914995 1 gene III (ORF) incomplete range1914995 1 1656 2302 annotation1914991 1 rbs-g9 range1914991 1 1190 1205 annotation1914985 1 rbs-g10 range1914985 1 480 495 annotation1915178 1 BamHI range1915178 1 1307 1311 annotation1914990 1 promoter-g8 range1914990 1 1317 1364 BBa_M31513_sequence 1 aacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtaattcacaatgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgttccagctaagtaacatggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaacctccgttgtactttgtttcgcgcttggtataatcgctgggggtcaaagatgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttaatgtaaacttcctcacgaaaggatccggccgaatctccgttgtactttgtttcgcgcttggtataatcgctgggggtctaatggaaacttcctcatgaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattcacctcgaaagcaagctgataaaccgatacaattaaaggctccttttggagcctttttttttggagattttcaacgtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccgcctggtactgagcaaaaccccgctaatcctaatccttctcttgaggagtctcagcctcttaatactttcatgtttcagaataataggttccgaaataggcagggggcattaactgtttatacgggcactgttactcaaggcactgaccccgttaaaacttattaccagtacactcctgtatcatcaaaagccatgtatgacgcttactggaacggtaaattcagagactgcgctttccattctggctttaatgaggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z