LOCUS       BBa_M31516              2225 bp    DNA     linear       09-May-2024
DEFINITION  Refactored portion of M13 genome from unique Hpal site on gene II 
            to the BamHI site on gene III
ACCESSION   BBa_M31516
VERSION     BBa_M31516.1  
FEATURES             Location/Qualifiers
     promoter        381..428
                     /label=promoter-g10
     RBS             480..495
                     /label=rbs-g10
     promoter        786..835
                     /label=promoter-g5
     RBS             827..842
                     /label=rbs-g5
     CDS             843..1106
                     /label=g5
     misc_feature    1021..1025
                     /label=BsrGI
     RBS             1092..1107
                     /label=rbs-g7
     promoter        1155..1201
                     /label=promoter-g8
     RBS             1190..1205
                     /label=rbs-g9
     CDS             1301..1522
                     /label=g8
     promoter        1500..1547
                     /label=promoter-g3
     RBS             1563..1578
                     /label=rbs-g3
     CDS             1579..2225
                     /label=g3 (incomplete)
     misc_feature    2220..2224
                     /label=BamHI
     misc_feature    1..3
                     /label=HpaI (incomplete)
     CDS             1..837
                     /label=g2 (incomplete)
     CDS             496..831
                     /label=g10
     misc_feature    1268..1273
                     /label=SnaBI
ORIGIN
        1 aacgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
       61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
      121 cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
      181 gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
      241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
      301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
      361 tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
      421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
      481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
      541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
      601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
      661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
      721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
      781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
      841 caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
      901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
      961 aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
     1021 tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
     1081 gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
     1141 caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
     1201 caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
     1261 gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct
     1321 caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
     1381 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
     1441 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
     1501 attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
     1561 tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc
     1621 tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca
     1681 tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggttgt
     1741 ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca
     1801 tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
     1861 tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
     1921 attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
     1981 aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
     2041 cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact
     2101 caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
     2161 tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgag
     2221 gatcc
//