BBa_M31516 1 BBa_M31516 Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III 2007-02-26T12:00:00Z 2015-05-08T01:14:00Z Sequence taken from the copy of the M13K07 genome available at: http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt Contains refactored sequences of the M13 genome from HpaI [8669/8669] - BamHI [2220/2224]. false false _102_ 0 1377 102 Not in stock false We wanted to refactor the genome of the M13 to faciliate future engineering. In doing so, we want to "clean up" the wildtype genes so that they are independent of each other and can be easily engineered independent of each other. We also want to delete unnecessary or obscure sequences for better human understanding of the M13 genome. false Han Zhu annotation1914416 1 promoter-g5 range1914416 1 786 835 annotation1914426 1 g3 (incomplete) range1914426 1 1579 2225 annotation1914419 1 BsrGI range1914419 1 1021 1025 annotation1914418 1 g5 range1914418 1 843 1106 annotation1914425 1 rbs-g3 range1914425 1 1563 1578 annotation1914424 1 promoter-g3 range1914424 1 1500 1547 annotation1915025 1 SnaBI range1915025 1 1268 1273 annotation1914415 1 rbs-g10 range1914415 1 480 495 annotation1914429 1 HpaI (incomplete) range1914429 1 1 3 annotation1915023 1 g2 (incomplete) range1915023 1 1 837 annotation1914427 1 BamHI range1914427 1 2220 2224 annotation1914423 1 g8 range1914423 1 1301 1522 annotation1914422 1 rbs-g9 range1914422 1 1190 1205 annotation1915024 1 g10 range1915024 1 496 831 annotation1914417 1 rbs-g5 range1914417 1 827 842 annotation1914420 1 rbs-g7 range1914420 1 1092 1107 annotation1914414 1 promoter-g10 range1914414 1 381 428 annotation1914421 1 promoter-g8 range1914421 1 1155 1201 BBa_M31516_sequence 1 aacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtaattcacaatgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgttccggctaagtaacatggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatctccgttgtactttgtttcgcgcttggtataatcgctgggggtcaaagatgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttaatggaaacttcctcatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattcacctcgaaagcaagctgataaaccgatacaattaaaggctccttttggagcctttttttttggagattttcaacgtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccgcctggtactgagcaaaaccccgctaatcctaatccttctcttgaggagtctcagcctcttaatactttcatgtttcagaataataggttccgaaataggcagggggcattaactgtttatacgggcactgttactcaaggcactgaccccgttaaaacttattaccagtacactcctgtatcatcaaaagccatgtatgacgcttactggaacggtaaattcagagactgcgctttccattctggctttaatgaggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z