LOCUS BBa_M31518 2922 bp DNA linear 23-Dec-2024 DEFINITION Refactored M13K07 from Hpa1 cut site to BamH1 cut site ACCESSION BBa_M31518 VERSION BBa_M31518.1 FEATURES Location/Qualifiers CDS 1..831 /label=gene2 (ORF) promoter 129..148 /label=promoter g5 promoter 381..428 /label=promoter-g10 RBS 480..495 /label=rbs-g10 CDS 496..831 /label=gene10 (ORF) RBS 827..842 /label=rbs-g5 CDS 843..1106 /label=g5 (ORF) misc_feature 1021..1025 /label=BsrGI RBS 1092..1107 /label=rbs-gVII CDS 1108..1209 /label=gene VII promoter 1155..1201 /label=promoter-g8 RBS 1190..1205 /label=rbs-g9 CDS 1206..1304 /label=gene IX misc_feature 1268..1273 /label=SnaBI RBS 1285..1300 /label=rbs-g8 CDS 1301..1522 /label=g8 (ORF) misc_feature 1523..1528 /label=ecoRI misc_feature 1529..1534 /label=XmaI promoter 1535..1582 /label=promoter-g3 RBS 1598..1613 /label=rbs-g3_add CDS 1614..2255 /label=gene III_add RBS 2265..2280 /label=rbs-g3 CDS 2281..2922 /label=gene III (ORF) misc_feature 2922..2922 /label=BamHI promoter 3086..3132 /label=promoter-g1 CDS 3196..4242 /label=g1 RBS 3900..3915 /label=rbs-g11 promoter 4055..4103 /label=promoter-g4 misc_feature 4131..4138 /label=PacI RBS 4204..4219 /label=rbs g-4 CDS 4220..5500 /label=g4 misc_feature 4743..4748 /label=ApaLI misc_feature 5080..5085 /label=MscI misc_feature 5613..5617 /label=NgoMIV misc_feature 5763..5765 /label=DrdI misc_feature 5867..5871 /label=SgrAI misc_feature 6030..6035 /label=SacII misc_feature 6158..6162 /label=BssSI misc_feature 6518..6520 /label=BstBI misc_feature 6824..6834 /label=AhdI misc_feature 6951..6956 /label=SacI misc_feature 7402..7406 /label=HindIII misc_feature 7402..7407 /label=HindIII misc_feature 7504..7508 /label=BsmBI misc_feature 7524..7526 /label=PvuI misc_feature 7611..7612 /label=EcoNI misc_feature 7865..7870 /label=NruI misc_feature 7922..7926 /label=TliI misc_feature 7922..7926 /label=XhoI misc_feature 8079..8083 /label=Pst1 promoter 8188..8235 /label=promoter-g2 misc_feature 8202..8209 /label=SwaI RBS 8252..8267 /label=rbs-g2 RBS 8252..8267 /label=rbs-g1 misc_feature 8353..8358 /label=AccI misc_feature 1..1 /label=HpaI ORIGIN 1 aacgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat 61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact 121 cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta 181 gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca 241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg 301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag 361 tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt 421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca 481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct 541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt 601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt 661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg 721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt 781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca 841 caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt 901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg 961 aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc 1021 tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc 1081 gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat 1141 caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt 1201 caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta 1261 gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct 1321 caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga 1381 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta 1441 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa 1501 atttacctcg aaagcaagtt gagaattccc cgggaattca cctcgaaagc aagctgataa 1561 accgatacaa ttaaaggctc cttttggagc cttttttttt ggagattttc aacgtgaaaa 1621 aattattatt cgcaattcct ttagttgttc ctttctattc tcactccgct gaaactgttg 1681 aaagttgttt agcaaaaccc catacagaaa attcatttac taacgtctgg aaagacgaca 1741 aaactttaga tcgttacgct aactatgagg gttgtctgtg gaatgctaca ggcgttgtag 1801 tttgtactgg tgacgaaact cagtgttacg gtacatgggt tcctattggg cttgctatcc 1861 ctgaaaatga gggtggtggc tctgagggtg gcggttctga gggtggcggt tctgagggtg 1921 gcggtactaa acctcctgag tacggtgata cacctattcc gggctatact tatatcaacc 1981 ctctcgacgg cacttatccg cctggtactg agcaaaaccc cgctaatcct aatccttctc 2041 ttgaggagtc tcagcctctt aatactttca tgtttcagaa taataggttc cgaaataggc 2101 agggggcatt aactgtttat acgggcactg ttactcaagg cactgacccc gttaaaactt 2161 attaccagta cactcctgta tcatcaaaag ccatgtatga cgcttactgg aacggtaaat 2221 tcagagactg cgctttccat tctggcttta atgaggcctt tttttttgga gattttcaac 2281 gtgaaaaaat tattattcgc aattccttta gttgttcctt tctattctca ctccgctgaa 2341 actgttgaaa gttgtttagc aaaaccccat acagaaaatt catttactaa cgtctggaaa 2401 gacgacaaaa ctttagatcg ttacgctaac tatgagggtt gtctgtggaa tgctacaggc 2461 gttgtagttt gtactggtga cgaaactcag tgttacggta catgggttcc tattgggctt 2521 gctatccctg aaaatgaggg tggtggctct gagggtggcg gttctgaggg tggcggttct 2581 gagggtggcg gtactaaacc tcctgagtac ggtgatacac ctattccggg ctatacttat 2641 atcaaccctc tcgacggcac ttatccgcct ggtactgagc aaaaccccgc taatcctaat 2701 ccttctcttg aggagtctca gcctcttaat actttcatgt ttcagaataa taggttccga 2761 aataggcagg gggcattaac tgtttatacg ggcactgtta ctcaaggcac tgaccccgtt 2821 aaaacttatt accagtacac tcctgtatca tcaaaagcca tgtatgacgc ttactggaac 2881 ggtaaattca gagactgcgc tttccattct ggctttaatg ag //