BBa_M31518 1 BBa_M31518 Refactored M13K07 from Hpa1 cut site to BamH1 cut site 2007-02-27T12:00:00Z 2015-05-08T01:14:00Z M13K07 genome Refactored part of the M13K07 genome from the Hpa1 cut site to the BamH1 cut site. It is designed to not contain overlaps of DNA elements. Also, each gene has a particular function assigned to it. It is also designed so that future manipulations can be made easier. false false _102_ 0 1367 102 Not in stock false Which restriction enzymes to include, which bases to mutate to eliminate overlaps of DNA elements. false Sophie Wong annotation1916219 1 AccI range1916219 1 8353 8358 annotation1916202 1 BssSI range1916202 1 6158 6162 annotation1916199 1 DrdI range1916199 1 5763 5765 annotation1916188 1 BamHI range1916188 1 2922 2922 annotation1916187 1 gene III (ORF) range1916187 1 2281 2922 annotation1916207 1 HindIII range1916207 1 7402 7407 annotation1916177 1 gene IX range1916177 1 1206 1304 annotation1916196 1 ApaLI range1916196 1 4743 4748 annotation1916166 1 promoter g5 range1916166 1 129 148 annotation1916203 1 BstBI range1916203 1 6518 6520 annotation1916173 1 rbs-gVII range1916173 1 1092 1107 annotation1916175 1 promoter-g8 range1916175 1 1155 1201 annotation1916195 1 g4 range1916195 1 4220 5500 annotation1916167 1 promoter-g10 range1916167 1 381 428 annotation1916197 1 MscI range1916197 1 5080 5085 annotation1916172 1 BsrGI range1916172 1 1021 1025 annotation1916217 1 rbs-g2 range1916217 1 8252 8267 annotation1916171 1 g5 (ORF) range1916171 1 843 1106 annotation1916165 1 gene2 (ORF) range1916165 1 1 831 annotation1916191 1 rbs-g11 range1916191 1 3900 3915 annotation1916186 1 rbs-g3 range1916186 1 2265 2280 annotation1916216 1 SwaI range1916216 1 8202 8209 annotation1916169 1 gene10 (ORF) range1916169 1 496 831 annotation1916210 1 EcoNI range1916210 1 7611 7612 annotation1916193 1 PacI range1916193 1 4131 4138 annotation1916183 1 promoter-g3 range1916183 1 1535 1582 annotation1916204 1 AhdI range1916204 1 6824 6834 annotation1916206 1 HindIII range1916206 1 7402 7406 annotation1916213 1 XhoI range1916213 1 7922 7926 annotation1916209 1 PvuI range1916209 1 7524 7526 annotation1916176 1 rbs-g9 range1916176 1 1190 1205 annotation1916208 1 BsmBI range1916208 1 7504 7508 annotation1916174 1 gene VII range1916174 1 1108 1209 annotation1916178 1 SnaBI range1916178 1 1268 1273 annotation1916214 1 Pst1 range1916214 1 8079 8083 annotation1916179 1 rbs-g8 range1916179 1 1285 1300 annotation1916184 1 rbs-g3_add range1916184 1 1598 1613 annotation1916190 1 g1 range1916190 1 3196 4242 annotation1916220 1 HpaI range1916220 1 1 1 annotation1916182 1 XmaI range1916182 1 1529 1534 annotation1916185 1 gene III_add range1916185 1 1614 2255 annotation1916180 1 g8 (ORF) range1916180 1 1301 1522 annotation1916170 1 rbs-g5 range1916170 1 827 842 annotation1916168 1 rbs-g10 range1916168 1 480 495 annotation1916205 1 SacI range1916205 1 6951 6956 annotation1916181 1 ecoRI range1916181 1 1523 1528 annotation1916215 1 promoter-g2 range1916215 1 8188 8235 annotation1916212 1 TliI range1916212 1 7922 7926 annotation1916189 1 promoter-g1 range1916189 1 3086 3132 annotation1916198 1 NgoMIV range1916198 1 5613 5617 annotation1916201 1 SacII range1916201 1 6030 6035 annotation1916192 1 promoter-g4 range1916192 1 4055 4103 annotation1916218 1 rbs-g1 range1916218 1 8252 8267 annotation1916211 1 NruI range1916211 1 7865 7870 annotation1916200 1 SgrAI range1916200 1 5867 5871 annotation1916194 1 rbs g-4 range1916194 1 4204 4219 BBa_M31518_sequence 1 aacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtaattcacaatgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgttccggctaagtaacatggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatctccgttgtactttgtttcgcgcttggtataatcgctgggggtcaaagatgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttaatggaaacttcctcatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaatttacctcgaaagcaagttgagaattccccgggaattcacctcgaaagcaagctgataaaccgatacaattaaaggctccttttggagcctttttttttggagattttcaacgtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccgcctggtactgagcaaaaccccgctaatcctaatccttctcttgaggagtctcagcctcttaatactttcatgtttcagaataataggttccgaaataggcagggggcattaactgtttatacgggcactgttactcaaggcactgaccccgttaaaacttattaccagtacactcctgtatcatcaaaagccatgtatgacgcttactggaacggtaaattcagagactgcgctttccattctggctttaatgaggcctttttttttggagattttcaacgtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccgcctggtactgagcaaaaccccgctaatcctaatccttctcttgaggagtctcagcctcttaatactttcatgtttcagaataataggttccgaaataggcagggggcattaactgtttatacgggcactgttactcaaggcactgaccccgttaaaacttattaccagtacactcctgtatcatcaaaagccatgtatgacgcttactggaacggtaaattcagagactgcgctttccattctggctttaatgag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z