BBa_M31518
type: ComponentDefinition
persistent identity: BBa M31518
display id: BBa_M31518
version: 1
was generated by: iGEM to SBOL conversion
description: Refactored M13K07 from Hpa1 cut site to BamH1 cut site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: M13K07 genome
owned by: james
owned by: myers
mutable description: Refactored part of the M13K07 genome from the Hpa1 cut site to the BamH1 cut site. It is designed to not contain overlaps of DNA elements. Also, each gene has a particular function assigned to it. It is also designed so that future manipulations can be made easier.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1367
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Which restriction enzymes to include, which bases to mutate to eliminate overlaps of DNA elements.
star: false
creator: Sophie Wong
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: HpaI
sequence annotation: gene2 (ORF)
sequence annotation: promoter g5
sequence annotation: promoter-g10
sequence annotation: rbs-g10
sequence annotation: gene10 (ORF)
sequence annotation: rbs-g5
sequence annotation: g5 (ORF)
sequence annotation: BsrGI
sequence annotation: rbs-gVII
sequence annotation: gene VII
sequence annotation: promoter-g8
sequence annotation: rbs-g9
sequence annotation: gene IX
sequence annotation: SnaBI
sequence annotation: rbs-g8
sequence annotation: g8 (ORF)
sequence annotation: ecoRI
sequence annotation: XmaI
sequence annotation: promoter-g3
sequence annotation: rbs-g3_add
sequence annotation: gene III_add
sequence annotation: rbs-g3
sequence annotation: gene III (ORF)
sequence annotation: BamHI
sequence annotation: promoter-g1
sequence annotation: g1
sequence annotation: rbs-g11
sequence annotation: promoter-g4
sequence annotation: PacI
sequence annotation: rbs g-4
sequence annotation: g4
sequence annotation: ApaLI
sequence annotation: MscI
sequence annotation: NgoMIV
sequence annotation: DrdI
sequence annotation: SgrAI
sequence annotation: SacII
sequence annotation: BssSI
sequence annotation: BstBI
sequence annotation: AhdI
sequence annotation: SacI
sequence annotation: HindIII
sequence annotation: BsmBI
sequence annotation: PvuI
sequence annotation: EcoNI
sequence annotation: NruI
sequence annotation: TliI
sequence annotation: XhoI
sequence annotation: Pst1
sequence annotation: promoter-g2

HpaI
type: SequenceAnnotation
persistent identity: annotation1916220
display id: annotation1916220
version: 1
top level: BBa_M31518
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role: feature/misc
role: SO:0000110

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display id: range1916220
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start: 1
end: 1
orientation: inline

gene2 (ORF)
type: SequenceAnnotation
persistent identity: annotation1916165
display id: annotation1916165
version: 1
top level: BBa_M31518
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role: feature/protein
role: SO:0000316

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display id: range1916165
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orientation: inline

promoter g5
type: SequenceAnnotation
persistent identity: annotation1916166
display id: annotation1916166
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916166/range1916166/1
role: feature/promoter
role: SO:0000167

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display id: range1916166
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end: 148
orientation: inline

promoter-g10
type: SequenceAnnotation
persistent identity: annotation1916167
display id: annotation1916167
version: 1
top level: BBa_M31518
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role: feature/promoter
role: SO:0000167

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display id: range1916167
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start: 381
end: 428
orientation: inline

rbs-g10
type: SequenceAnnotation
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display id: annotation1916168
version: 1
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role: feature/rbs
role: SO:0000139

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display id: range1916168
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end: 495
orientation: inline

gene10 (ORF)
type: SequenceAnnotation
persistent identity: annotation1916169
display id: annotation1916169
version: 1
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role: feature/protein
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display id: range1916169
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orientation: inline

rbs-g5
type: SequenceAnnotation
persistent identity: annotation1916170
display id: annotation1916170
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916170/range1916170/1
role: feature/rbs
role: SO:0000139

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persistent identity: range1916170
display id: range1916170
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end: 842
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g5 (ORF)
type: SequenceAnnotation
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display id: annotation1916171
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role: SO:0000316
role: feature/protein

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persistent identity: range1916171
display id: range1916171
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end: 1106
orientation: inline

BsrGI
type: SequenceAnnotation
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display id: annotation1916172
version: 1
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role: feature/misc
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persistent identity: range1916172
display id: range1916172
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rbs-gVII
type: SequenceAnnotation
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display id: annotation1916173
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916173/range1916173/1
role: feature/rbs
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persistent identity: range1916173
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orientation: inline

gene VII
type: SequenceAnnotation
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display id: annotation1916174
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location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916174/range1916174/1
role: SO:0000316
role: feature/protein

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persistent identity: range1916174
display id: range1916174
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end: 1209
orientation: inline

promoter-g8
type: SequenceAnnotation
persistent identity: annotation1916175
display id: annotation1916175
version: 1
top level: BBa_M31518
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role: feature/promoter
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persistent identity: range1916175
display id: range1916175
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orientation: inline

rbs-g9
type: SequenceAnnotation
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display id: annotation1916176
version: 1
top level: BBa_M31518
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role: SO:0000139
role: feature/rbs

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persistent identity: range1916176
display id: range1916176
version: 1
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orientation: inline

gene IX
type: SequenceAnnotation
persistent identity: annotation1916177
display id: annotation1916177
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916177/range1916177/1
role: SO:0000316
role: feature/protein

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type: Range
persistent identity: range1916177
display id: range1916177
version: 1
top level: BBa_M31518
direction: direction/forward
start: 1206
end: 1304
orientation: inline

SnaBI
type: SequenceAnnotation
persistent identity: annotation1916178
display id: annotation1916178
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916178/range1916178/1
role: feature/misc
role: SO:0000110

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type: Range
persistent identity: range1916178
display id: range1916178
version: 1
top level: BBa_M31518
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start: 1268
end: 1273
orientation: inline

rbs-g8
type: SequenceAnnotation
persistent identity: annotation1916179
display id: annotation1916179
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916179/range1916179/1
role: feature/rbs
role: SO:0000139

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type: Range
persistent identity: range1916179
display id: range1916179
version: 1
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end: 1300
orientation: inline

g8 (ORF)
type: SequenceAnnotation
persistent identity: annotation1916180
display id: annotation1916180
version: 1
top level: BBa_M31518
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role: feature/protein
role: SO:0000316

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persistent identity: range1916180
display id: range1916180
version: 1
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orientation: inline

ecoRI
type: SequenceAnnotation
persistent identity: annotation1916181
display id: annotation1916181
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916181/range1916181/1
role: feature/misc
role: SO:0000110

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type: Range
persistent identity: range1916181
display id: range1916181
version: 1
top level: BBa_M31518
direction: direction/forward
start: 1523
end: 1528
orientation: inline

XmaI
type: SequenceAnnotation
persistent identity: annotation1916182
display id: annotation1916182
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916182/range1916182/1
role: SO:0000110
role: feature/misc

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type: Range
persistent identity: range1916182
display id: range1916182
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end: 1534
orientation: inline

promoter-g3
type: SequenceAnnotation
persistent identity: annotation1916183
display id: annotation1916183
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916183/range1916183/1
role: SO:0000167
role: feature/promoter

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type: Range
persistent identity: range1916183
display id: range1916183
version: 1
top level: BBa_M31518
direction: direction/forward
start: 1535
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orientation: inline

rbs-g3_add
type: SequenceAnnotation
persistent identity: annotation1916184
display id: annotation1916184
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916184/range1916184/1
role: feature/rbs
role: SO:0000139

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type: Range
persistent identity: range1916184
display id: range1916184
version: 1
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orientation: inline

gene III_add
type: SequenceAnnotation
persistent identity: annotation1916185
display id: annotation1916185
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916185/range1916185/1
role: feature/protein
role: SO:0000316

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orientation: inline

rbs-g3
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role: feature/rbs
role: SO:0000139

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display id: range1916186
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gene III (ORF)
type: SequenceAnnotation
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display id: annotation1916187
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role: feature/protein

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BamHI
type: SequenceAnnotation
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display id: annotation1916188
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promoter-g1
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g1
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rbs-g11
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promoter-g4
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PacI
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rbs g-4
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g4
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ApaLI
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MscI
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NgoMIV
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DrdI
type: SequenceAnnotation
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SgrAI
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SacII
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BssSI
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display id: annotation1916202
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916202/range1916202/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31518/annotation1916202/range1916202/1
type: Range
persistent identity: range1916202
display id: range1916202
version: 1
top level: BBa_M31518
direction: direction/forward
start: 6158
end: 6162
orientation: inline

BstBI
type: SequenceAnnotation
persistent identity: annotation1916203
display id: annotation1916203
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916203/range1916203/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31518/annotation1916203/range1916203/1
type: Range
persistent identity: range1916203
display id: range1916203
version: 1
top level: BBa_M31518
direction: direction/forward
start: 6518
end: 6520
orientation: inline

AhdI
type: SequenceAnnotation
persistent identity: annotation1916204
display id: annotation1916204
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916204/range1916204/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31518/annotation1916204/range1916204/1
type: Range
persistent identity: range1916204
display id: range1916204
version: 1
top level: BBa_M31518
direction: direction/forward
start: 6824
end: 6834
orientation: inline

SacI
type: SequenceAnnotation
persistent identity: annotation1916205
display id: annotation1916205
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916205/range1916205/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31518/annotation1916205/range1916205/1
type: Range
persistent identity: range1916205
display id: range1916205
version: 1
top level: BBa_M31518
direction: direction/forward
start: 6951
end: 6956
orientation: inline

HindIII
type: SequenceAnnotation
persistent identity: annotation1916206
display id: annotation1916206
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916206/range1916206/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31518/annotation1916206/range1916206/1
type: Range
persistent identity: range1916206
display id: range1916206
version: 1
top level: BBa_M31518
direction: direction/forward
start: 7402
end: 7406
orientation: inline

HindIII
type: SequenceAnnotation
persistent identity: annotation1916207
display id: annotation1916207
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916207/range1916207/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31518/annotation1916207/range1916207/1
type: Range
persistent identity: range1916207
display id: range1916207
version: 1
top level: BBa_M31518
direction: direction/forward
start: 7402
end: 7407
orientation: inline

BsmBI
type: SequenceAnnotation
persistent identity: annotation1916208
display id: annotation1916208
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916208/range1916208/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31518/annotation1916208/range1916208/1
type: Range
persistent identity: range1916208
display id: range1916208
version: 1
top level: BBa_M31518
direction: direction/forward
start: 7504
end: 7508
orientation: inline

PvuI
type: SequenceAnnotation
persistent identity: annotation1916209
display id: annotation1916209
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916209/range1916209/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31518/annotation1916209/range1916209/1
type: Range
persistent identity: range1916209
display id: range1916209
version: 1
top level: BBa_M31518
direction: direction/forward
start: 7524
end: 7526
orientation: inline

EcoNI
type: SequenceAnnotation
persistent identity: annotation1916210
display id: annotation1916210
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916210/range1916210/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31518/annotation1916210/range1916210/1
type: Range
persistent identity: range1916210
display id: range1916210
version: 1
top level: BBa_M31518
direction: direction/forward
start: 7611
end: 7612
orientation: inline

NruI
type: SequenceAnnotation
persistent identity: annotation1916211
display id: annotation1916211
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916211/range1916211/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31518/annotation1916211/range1916211/1
type: Range
persistent identity: range1916211
display id: range1916211
version: 1
top level: BBa_M31518
direction: direction/forward
start: 7865
end: 7870
orientation: inline

TliI
type: SequenceAnnotation
persistent identity: annotation1916212
display id: annotation1916212
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916212/range1916212/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31518/annotation1916212/range1916212/1
type: Range
persistent identity: range1916212
display id: range1916212
version: 1
top level: BBa_M31518
direction: direction/forward
start: 7922
end: 7926
orientation: inline

XhoI
type: SequenceAnnotation
persistent identity: annotation1916213
display id: annotation1916213
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916213/range1916213/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31518/annotation1916213/range1916213/1
type: Range
persistent identity: range1916213
display id: range1916213
version: 1
top level: BBa_M31518
direction: direction/forward
start: 7922
end: 7926
orientation: inline

Pst1
type: SequenceAnnotation
persistent identity: annotation1916214
display id: annotation1916214
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916214/range1916214/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31518/annotation1916214/range1916214/1
type: Range
persistent identity: range1916214
display id: range1916214
version: 1
top level: BBa_M31518
direction: direction/forward
start: 8079
end: 8083
orientation: inline

promoter-g2
type: SequenceAnnotation
persistent identity: annotation1916215
display id: annotation1916215
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916215/range1916215/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M31518/annotation1916215/range1916215/1
type: Range
persistent identity: range1916215
display id: range1916215
version: 1
top level: BBa_M31518
direction: direction/forward
start: 8188
end: 8235
orientation: inline

SwaI
type: SequenceAnnotation
persistent identity: annotation1916216
display id: annotation1916216
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916216/range1916216/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31518/annotation1916216/range1916216/1
type: Range
persistent identity: range1916216
display id: range1916216
version: 1
top level: BBa_M31518
direction: direction/forward
start: 8202
end: 8209
orientation: inline

rbs-g2
type: SequenceAnnotation
persistent identity: annotation1916217
display id: annotation1916217
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916217/range1916217/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_M31518/annotation1916217/range1916217/1
type: Range
persistent identity: range1916217
display id: range1916217
version: 1
top level: BBa_M31518
direction: direction/forward
start: 8252
end: 8267
orientation: inline

rbs-g1
type: SequenceAnnotation
persistent identity: annotation1916218
display id: annotation1916218
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916218/range1916218/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_M31518/annotation1916218/range1916218/1
type: Range
persistent identity: range1916218
display id: range1916218
version: 1
top level: BBa_M31518
direction: direction/forward
start: 8252
end: 8267
orientation: inline

AccI
type: SequenceAnnotation
persistent identity: annotation1916219
display id: annotation1916219
version: 1
top level: BBa_M31518
location: https://synbiohub.org/public/igem/BBa_M31518/annotation1916219/range1916219/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31518/annotation1916219/range1916219/1
type: Range
persistent identity: range1916219
display id: range1916219
version: 1
top level: BBa_M31518
direction: direction/forward
start: 8353
end: 8358
orientation: inline

https://synbiohub.org/public/igem/BBa_M31518_sequence/1
type: Sequence
persistent identity: BBa M31518 sequence
display id: BBa_M31518_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtaattcacaatgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgttccggctaagtaacatggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatctccgttgtactttgtttcgcgcttggtataatcgctgggggtcaaagatgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttaatggaaacttcctcatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaatttacctcgaaagcaagttgagaattccccgggaattcacctcgaaagcaagctgataaaccgatacaattaaaggctccttttggagcctttttttttggagattttcaacgtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccg…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z