BBa_M31530
type: ComponentDefinition
persistent identity: BBa M31530
display id: BBa_M31530
version: 1
was generated by: iGEM to SBOL conversion
description: Refactored Portion of M13K07 Genome
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: This part does not yet exist in a physical form, though it may be synthesized in the near future.
owned by: james
owned by: myers
mutable description: I've refactored the segment of DNA between the unique HapI and BamHI sites. Following the guidlines in "Refactoring T7" by Chan et al, I've determined what functional components that I would like to be physically separated from one another (namely regulatory regions and ORFs)and set them apart in my design. I'm not sure that these modifications will yield a functional phage. Even viable phage result, I don't know what sort of emergent functions I will have created by refactoring the genome.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1380
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: In order to organize the M13K07 genome into individual components, some sequences must be "copied and pasted" elsewhere. This opens the possibility for recomination events or coding regions that code for functions for which they are no longer responsible (legacy functions). These interactions must be addressed to avoid interactions bewteen stretches of DNA. As far as I know, this can be addressed by changing codon usage, introducing inhibitory secondary structures to the DNA, or by eliminating the enzymatic mechanisms by which these events may occur. I'm sure there are others. Another consideration is that absolute and relative position in the genome may be of importance. I will try to maintain the "localities" as best I can so as not to introduce further sources of problems.
star: false
creator: Matt Gethers
type: DnaRegion
role: SO:0000804
role: partType/Composite
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component: BBa_M13502
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component: BBa_M13103
component: BBa_M13008
component: BBa_M13508
component: BBa_M13108
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sequence annotation: BBa_M13108
sequence annotation: BBa_M13508
sequence annotation: BBa_M13008
sequence annotation: BBa_M13103
sequence annotation: BBa_M13503
sequence annotation: BBa_M13003
sequence: https://synbiohub.org/public/igem/BBa_M31530_sequence/1

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BBa_M13503
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BBa_M13003
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BBa_M13010
type: ComponentDefinition
persistent identity: BBa M13010
display id: BBa_M13010
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene X
created: 2006-12-05T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome. It encodes a protein that is important for phage maturation. The protein remains in the host cytoplasm to interact with the product of gI and the OM pore (product of gIV) through which the phage ssDNA is threaded as the phage matures. GeneX protein is identical to the C-terminal portion of the gIprotein since gXprotien is normally encoded through an internal initiation of translation from the gI transcript.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: need to unstuff from inside gI
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M13010_sequence/1

BBa_M13005
type: ComponentDefinition
persistent identity: BBa M13005
display id: BBa_M13005
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene V
created: 2006-12-11T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome. It encodes a single stranded DNA binding protein that is important for phage maturation.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: RBS for gene V overlaps stop codon for upstream phage genes II and X.
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_M13005_sequence/1

BBa_M13007
type: ComponentDefinition
persistent identity: BBa M13007
display id: BBa_M13007
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene VII
created: 2006-12-11T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome. It encodes a protein that is presented with p9 to initiate phage assembly at the blunt end of the phage coat. Approximately 5 copies of each protein are presented on mature phage. Without p9 or p7, no phage is produced. p7 is one of the smallest ribosomally translated proteins with described function. It is hydrophobic and the N-terminus is negatively charged, suggesting a C-terminus inside orientation.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: stop codon overlaps with p9 start codon in M13 genome.
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_M13007_sequence/1

BBa_M13008
type: ComponentDefinition
persistent identity: BBa M13008
display id: BBa_M13008
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene VIII
created: 2006-12-12T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome. It encodes the major coat protein, with approximately 2700 copies of the protein on each phage particle. The protein is first synthesized at a longer precursor protein that is clipped between the Alanines at positions 23 and 24. It is possible to silently add a Pst site to clone fusions upstream and in frame with Ala that begins mature protein. Small peptide-fusions are possible to the N-terminal portion of the mature p8 (phage display systems are built around this finding) but addition of more than 6 or 8 residues makes the phage pack poorly, leading to unproductive infections.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Many! The start codon for gene 8 overlaps with the stop codon for the upstream gene 9. Might be nice to include cloning sites for directed N-terminal fusions to mature form of protein. Might be nice to codon vary a different biobrick of gene VIII to allow for two genes expressed on single genome, inhibiting recombination in ssDNA.
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_M13008_sequence/1

BBa_M13003
type: ComponentDefinition
persistent identity: BBa M13003
display id: BBa_M13003
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene III
created: 2006-12-13T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome, encoding a minor coat protein. Approximately 5 copies of p3 are presented with 5 copies of p6 on the rounded end of the mature phage. In the absence of p3, the phage remains tethered to the bacterial host, elongating to 10 or 20 times the normal phage length and incorporating multiple ssDNA genomes within the coat. p3 (domain II) is also responsible for the initial interaction of the phage with the bacterial pili during infection. Peptide fusions, sometimes large, can be displayed on N-terminal end of p3, though highly charged fusions are difficult to isolate since they are not easily secreted by the host secretion apparatus. The p3 protein is first synthesized as a longer precursor protein that is 18 amino acids longer than the mature protein. The gene's translation initiates with a GTG codon, and changing this codon to an ATG increases expression of gene III to levels with negative effects on phage maturation.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The RBS for gene III is within the transcription termination sequence of the upstream gVIII, thus modifications to termination affect gIII initiation. Translation initiates with a GTG rather than a canonical ATG. There is a unique, natural BamHI site in domain II that may be useful for cloning, though it may prevent interaction of p3 with pilus.
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_M13003_sequence/1

BBa_M13002
type: ComponentDefinition
persistent identity: BBa M13002
display id: BBa_M13002
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene II
created: 2006-12-13T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome. It encodes a protein that is important for phage DNA replication, initiating ssDNA synthesis of the (+) strand by nicking the RF near the M13 replication origin. p10 is encoded within gII thanks to a translation re-initiation event.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: need to unstuff gX from within coding sequence. RBS for downstream gV overlaps the gII stop codon.
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M13002_sequence/1

BBa_M13502
type: ComponentDefinition
persistent identity: BBa M13502
display id: BBa_M13502
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene II RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene II message (BBa_M13002). The part aligns with base pairs 8252-8267 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13502_sequence/1

BBa_M13510
type: ComponentDefinition
persistent identity: BBa M13510
display id: BBa_M13510
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene X RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene X message (BBa_M13010). The part aligns with base pairs 480-495 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13510_sequence/1

BBa_M13505
type: ComponentDefinition
persistent identity: BBa M13505
display id: BBa_M13505
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene V RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene V message (BBa_M13005). The part aligns with base pairs 827-842 in M13K07 genome. It was identified as the RBS for the gene V message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13505_sequence/1

BBa_M13507
type: ComponentDefinition
persistent identity: BBa M13507
display id: BBa_M13507
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene VII RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VII message (BBa_M13007). The part aligns with base pairs 1092-1107 in M13K07 genome. It was identified as the RBS for gene VII message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13507_sequence/1

BBa_M13509
type: ComponentDefinition
persistent identity: BBa M13509
display id: BBa_M13509
version: 1
was generated by: iGEM to SBOL conversion
description: M13Ko7 gene IX RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene IX message (BBa_M13009). The part aligns with base pairs 1190-1205 in M13K07 genome. It was identified as the RBS for gene IX message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13509_sequence/1

BBa_M13508
type: ComponentDefinition
persistent identity: BBa M13508
display id: BBa_M13508
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene VIII RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VIII message (BBa_M13008). The part aligns with base pairs 1285-1300 in M13K07 genome. It was identified as the RBS for gene VIII message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13508_sequence/1

BBa_M13503
type: ComponentDefinition
persistent identity: BBa M13503
display id: BBa_M13503
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene III RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene III message (BBa_M13003). The part aligns with base pairs 1563-1578 in M13K07 genome. It was identified as the RBS for gene III message by Wezenbeek et al in Gene (1980) 11: 129-148 New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13503_sequence/1

BBa_M13102
type: ComponentDefinition
persistent identity: BBa M13102
display id: BBa_M13102
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene II promoter
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome. It directs transcription of M13 gene II (BBa_M13502, BBa_M13002). Promoter identified in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13102_sequence/1

BBa_M13105
type: ComponentDefinition
persistent identity: BBa M13105
display id: BBa_M13105
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene V promoter
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 786-835 in M13K07. It directs transcription of M13 gene V (BBa_M13505, BBa_M13005). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13105_sequence/1

BBa_M13108
type: ComponentDefinition
persistent identity: BBa M13108
display id: BBa_M13108
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene VIII promoter
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 1155-1201 in M13K07. It directs transcription of M13 gene VIII (BBa_M13508, BBa_M13008). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13108_sequence/1

BBa_M13103
type: ComponentDefinition
persistent identity: BBa M13103
display id: BBa_M13103
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene III promoter
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 1500-1547 in M13K07. It directs transcription of M13 gene III (BBa_M13503, BBa_M13003). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_M13103_sequence/1

BBa_M13009
type: ComponentDefinition
persistent identity: BBa M13009
display id: BBa_M13009
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene IX
created: 2006-12-11T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome. It encodes a protein that is presented with p7 to initiate phage assembly at the blunt end of the phage coat. Approximately 5 copies of each protein are presented on mature phage. In the absence of p9 or p7, no phage is produced. p9 is one of the smallest ribosomally translated proteins with described function. N-terminal display is possible, and some positively charged residues are seen at the C-terminal end of the protein, suggesting a "C-terminus inside" orientation.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: start codon overlaps with stop codon of g7 upstream and stop codon overlaps with start codon of g8 downstream.
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_M13009_sequence/1

BBa_M13110
type: ComponentDefinition
persistent identity: BBa M13110
display id: BBa_M13110
version: 1
was generated by: iGEM to SBOL conversion
description: M13110
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 381-428 in M13K07. It directs transcription of M13 gene X (BBa_M13510, BBa_M13010). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_M13110_sequence/1

https://synbiohub.org/public/igem/BBa_M31530_sequence/1
type: Sequence
persistent identity: BBa M31530 sequence
display id: BBa_M31530_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tattaacgtttacaatttaaatatttgcttatacaatcttcctgttttatcaaccggggtacatatgattgacatgctagttttacgattaccgttcatcgattctcttgtttgctccagactctcaggcaatgacctgatagcctttgtagacctctcaaaaatagctaccctctccggcatgaatttatcagctagaacggttgaatatcatattgatggtgatttgactgtctccggcctttctcacccttttgaatctttacctacacattactcaggcattgcatttaaaatatatgagggttctaaaaatttttatccttgcgttgaaataaaggcttctcccgcaaaagtattacagggtcataatgtttttggtacaaccgatttagctttatgctctgaggctttattgcttaattttgctaattctttgccttgcctgtatgatttattggatgttaacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataaccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtacataaggtaattcacaatgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13010_sequence/1
type: Sequence
persistent identity: BBa M13010 sequence
display id: BBa_M13010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13005_sequence/1
type: Sequence
persistent identity: BBa M13005 sequence
display id: BBa_M13005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgttccggctaagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13007_sequence/1
type: Sequence
persistent identity: BBa M13007 sequence
display id: BBa_M13007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatctccgttgtactttgtttcgcgcttggtataatcgctgggggtcaaagatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13009_sequence/1
type: Sequence
persistent identity: BBa M13009 sequence
display id: BBa_M13009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttaatggaaacttcctcatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13008_sequence/1
type: Sequence
persistent identity: BBa M13008 sequence
display id: BBa_M13008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattcacctcgaaagcaagctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13003_sequence/1
type: Sequence
persistent identity: BBa M13003 sequence
display id: BBa_M13003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccgcctggtactgagcaaaaccccgctaatcctaatccttctcttgaggagtctcagcctcttaatactttcatgtttcagaataataggttccgaaataggcagggggcattaactgtttatacgggcactgttactcaaggcactgaccccgttaaaacttattaccagtacactcctgtatcatcaaaagccatgtatgacgcttactggaacggtaaattcagagactgcgctttccattctggctttaatgaggatccattcgtttgtgaatatcaaggccaatcgtctgacctgcctcaacctcctgtcaatgctggcggcggctctggtggtggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctctgagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacgctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaaacttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggccttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcggtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatcggttgaatgtcgcccttttgtctttagcgctggtaaaccatatgaattttctattgattgtgacaaaataaacttattccgtggtgtctttgcgtttcttttatatgttgccacctttatgtatgtattttctacgtttgctaacatactgcgtaataaggagtcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13002_sequence/1
type: Sequence
persistent identity: BBa M13002 sequence
display id: BBa_M13002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattgacatgctagttttacgattaccgttcatcgattctcttgtttgctccagactctcaggcaatgacctgatagcctttgtagacctctcaaaaatagctaccctctccggcatgaatttatcagctagaacggttgaatatcatattgatggtgatttgactgtctccggcctttctcacccttttgaatctttacctacacattactcaggcattgcatttaaaatatatgagggttctaaaaatttttatccttgcgttgaaataaaggcttctcccgcaaaagtattacagggtcataatgtttttggtacaaccgatttagctttatgctctgaggctttattgcttaattttgctaattctttgccttgcctgtatgatttattggatgttaacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13502_sequence/1
type: Sequence
persistent identity: BBa M13502 sequence
display id: BBa_M13502_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcaaccggggtacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13510_sequence/1
type: Sequence
persistent identity: BBa M13510 sequence
display id: BBa_M13510_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atttgagggggattca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13505_sequence/1
type: Sequence
persistent identity: BBa M13505 sequence
display id: BBa_M13505_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cataaggtaattcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13507_sequence/1
type: Sequence
persistent identity: BBa M13507 sequence
display id: BBa_M13507_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttccggctaagtaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13509_sequence/1
type: Sequence
persistent identity: BBa M13509 sequence
display id: BBa_M13509_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcgctgggggtcaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13508_sequence/1
type: Sequence
persistent identity: BBa M13508 sequence
display id: BBa_M13508_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatggaaacttcctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13503_sequence/1
type: Sequence
persistent identity: BBa M13503 sequence
display id: BBa_M13503_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttggagattttcaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13102_sequence/1
type: Sequence
persistent identity: BBa M13102 sequence
display id: BBa_M13102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tattaacgtttacaatttaaatatttgcttatacaatcttcctgtttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13110_sequence/1
type: Sequence
persistent identity: BBa M13110 sequence
display id: BBa_M13110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctttttgatgcaatccgctttgcttctgactataatagtcagggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13105_sequence/1
type: Sequence
persistent identity: BBa M13105 sequence
display id: BBa_M13105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13108_sequence/1
type: Sequence
persistent identity: BBa M13108 sequence
display id: BBa_M13108_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatctccgttgtactttgtttcgcgcttggtataatcgctgggggtc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13103_sequence/1
type: Sequence
persistent identity: BBa M13103 sequence
display id: BBa_M13103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattcacctcgaaagcaagctgataaaccgatacaattaaaggctcct
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z