BBa_M31532
1
BBa_M31532
This is gene X of M13K07 (Promoter, RBS, and ORF)
2007-02-28T12:00:00Z
2015-05-08T01:14:00Z
Though the gene and its regulatory sequences exist in nature in M13K07, they do not yet exist in this separate form.
When designing a phage, it is much more practical and effective to think of DNA in functional units than strings of regulatory and coding regions. This part combines the transcriptional and translational regions with the ORF to form a functional (where function is defined as the output of the gene).
false
false
_102_
0
1380
102
Not in stock
false
When removed from its place in the genome that has been determined by evolution, we can't be sure the change will be tolerated. There may be cross-talk between copied and separated elements with significant homology. Because we have removed and maintained entire portions of genes in the formation of separate genomic entities, there is also the possibility of expression of these 'partial' genes. I will address this by placing transcriptional terminators and hair pins between genes.
false
Matt Gethers
component1917904
1
BBa_M13010
component1917903
1
BBa_M13510
component1917902
1
BBa_M13110
annotation1917904
1
BBa_M13010
range1917904
1
65
400
annotation1917902
1
BBa_M13110
range1917902
1
1
48
annotation1917903
1
BBa_M13510
range1917903
1
49
64
BBa_M13110
1
BBa_M13110
M13110
2006-12-15T12:00:00Z
2015-05-08T01:13:55Z
New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
This part is from the bacteriophage M13 genome, bp 381-428 in M13K07. It directs transcription of M13 gene X (BBa_M13510, BBa_M13010). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
false
false
_45_
0
314
1
Not in stock
false
none
false
Natalie Kuldell
BBa_M13510
1
BBa_M13510
M13KO7 gene X RBS
2006-12-14T12:00:00Z
2015-05-08T01:13:56Z
New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
This RBS is part of the bacteriophage M13 genome, initiating translation of the gene X message (BBa_M13010). The part aligns with base pairs 480-495 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
false
false
_45_
0
314
1
Not in stock
false
none
false
Natalie Kuldell
BBa_M13010
1
BBa_M13010
M13K07 gene X
2006-12-05T12:00:00Z
2015-05-08T01:13:55Z
New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
This gene is part of the bacteriophage M13 genome. It encodes a protein that is important for phage maturation. The protein remains in the host cytoplasm to interact with the product of gI and the OM pore (product of gIV) through which the phage ssDNA is threaded as the phage matures. GeneX protein is identical to the C-terminal portion of the gIprotein since gXprotien is normally encoded through an internal initiation of translation from the gI transcript.
false
true
_45_
0
314
1
Not in stock
false
need to unstuff from inside gI
false
Natalie Kuldell
BBa_M31532_sequence
1
tctttttgatgcaatccgctttgcttctgactataatagtcagggtaaatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataa
BBa_M13110_sequence
1
tctttttgatgcaatccgctttgcttctgactataatagtcagggtaa
BBa_M13510_sequence
1
atttgagggggattca
BBa_M13010_sequence
1
atgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z