LOCUS       BBa_M31538              2414 bp    DNA     linear       17-May-2024
DEFINITION  This is the trio of genes in M13K07 responsible for Genome 
            Amplification
ACCESSION   BBa_M31538
VERSION     BBa_M31538.1  
FEATURES             Location/Qualifiers
     promoter        1..48
                     /label=BBa_M13102
     RBS             49..64
                     /label=BBa_M13502
     CDS             65..1297
                     /label=BBa_M13002
     misc_feature    1..1297
                     /label=BBa_M31531
     stem_loop       1309..1352
                     /label=stem_loop
     misc_feature    1298..1377
                     /label=BBa_B0010
     terminator      1298..1377
                     /label=BBa_B0010
     stem_loop       1393..1412
                     /label=T7 TE
     misc_feature    1419..1419
                     /label=stop
     polyA_site      1413..1426
                     /label=polya
     misc_feature    1386..1426
                     /label=BBa_B0012
     terminator      1386..1426
                     /label=BBa_B0012
     terminator      1298..1426
                     /label=BBa_B0015
     promoter        1427..1474
                     /label=BBa_M13110
     RBS             1475..1490
                     /label=BBa_M13510
     CDS             1491..1826
                     /label=BBa_M13010
     misc_feature    1427..1826
                     /label=BBa_M31532
     stem_loop       1838..1881
                     /label=stem_loop
     misc_feature    1827..1906
                     /label=BBa_B0010
     terminator      1827..1906
                     /label=BBa_B0010
     stem_loop       1922..1941
                     /label=T7 TE
     misc_feature    1948..1948
                     /label=stop
     polyA_site      1942..1955
                     /label=polya
     misc_feature    1915..1955
                     /label=BBa_B0012
     terminator      1915..1955
                     /label=BBa_B0012
     terminator      1827..1955
                     /label=BBa_B0015
     promoter        1956..2005
                     /label=BBa_M13105
     RBS             2006..2021
                     /label=BBa_M13505
     CDS             2022..2285
                     /label=BBa_M13005
     misc_feature    1956..2285
                     /label=BBa_M31533
     stem_loop       2297..2340
                     /label=stem_loop
     misc_feature    2286..2365
                     /label=BBa_B0010
     terminator      2286..2365
                     /label=BBa_B0010
     stem_loop       2381..2400
                     /label=T7 TE
     misc_feature    2407..2407
                     /label=stop
     polyA_site      2401..2414
                     /label=polya
     misc_feature    2374..2414
                     /label=BBa_B0012
     terminator      2374..2414
                     /label=BBa_B0012
     terminator      2286..2414
                     /label=BBa_B0015
ORIGIN
        1 tattaacgtt tacaatttaa atatttgctt atacaatctt cctgttttat caaccggggt
       61 acatatgatt gacatgctag ttttacgatt accgttcatc gattctcttg tttgctccag
      121 actctcaggc aatgacctga tagcctttgt agacctctca aaaatagcta ccctctccgg
      181 catgaattta tcagctagaa cggttgaata tcatattgat ggtgatttga ctgtctccgg
      241 cctttctcac ccttttgaat ctttacctac acattactca ggcattgcat ttaaaatata
      301 tgagggttct aaaaattttt atccttgcgt tgaaataaag gcttctcccg caaaagtatt
      361 acagggtcat aatgtttttg gtacaaccga tttagcttta tgctctgagg ctttattgct
      421 taattttgct aattctttgc cttgcctgta tgatttattg gatgttaacg ctactactat
      481 tagtagaatt gatgccacct tttcagctcg cgccccaaat gaaaatatag ctaaacaggt
      541 tattgaccat ttgcgaaatg tatctaatgg tcaaactaaa tctactcgtt cgcagaattg
      601 ggaatcaact gttacatgga atgaaacttc cagacaccgt actttagttg catatttaaa
      661 acatgttgag ctacagcacc agattcagca attaagctct aagccatccg caaaaatgac
      721 ctcttatcaa aaggagcaat taaaggtact ctctaatcct gacctgttgg agtttgcttc
      781 cggtctggtt cgctttgaag ctcgaattaa aacgcgatat ttgaagtctt tcgggcttcc
      841 tcttaatctt tttgatgcaa tccgctttgc ttctgactat aatagtcagg gtaaagacct
      901 gatttttgat ttatggtcat tctcgttttc tgaactgttt aaagcatttg agggggattc
      961 aatgaatatt tatgacgatt ccgcagtatt ggacgctatc cagtctaaac attttactat
     1021 taccccctct ggcaaaactt cttttgcaaa agcctctcgc tattttggtt tttatcgtcg
     1081 tctggtaaac gagggttatg atagtgttgc tcttactatg cctcgtaatt ccttttggcg
     1141 ttatgtatct gcattagttg aatgtggtat tcctaaatct caactgatga atctttctac
     1201 ctgtaataat gttgttccgt tagttcgttt tattaacgta gatttttctt cccaacgtcc
     1261 tgactggtat aatgagccag ttcttaaaat cgcataacca ggcatcaaat aaaacgaaag
     1321 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1381 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatctt tttgatgcaa
     1441 tccgctttgc ttctgactat aatagtcagg gtaaatttga gggggattca atgaatattt
     1501 atgacgattc cgcagtattg gacgctatcc agtctaaaca ttttactatt accccctctg
     1561 gcaaaacttc ttttgcaaaa gcctctcgct attttggttt ttatcgtcgt ctggtaaacg
     1621 agggttatga tagtgttgct cttactatgc ctcgtaattc cttttggcgt tatgtatctg
     1681 cattagttga atgtggtatt cctaaatctc aactgatgaa tctttctacc tgtaataatg
     1741 ttgttccgtt agttcgtttt attaacgtag atttttcttc ccaacgtcct gactggtata
     1801 atgagccagt tcttaaaatc gcataaccag gcatcaaata aaacgaaagg ctcagtcgaa
     1861 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1921 tggctcacct tcgggtgggc ctttctgcgt ttataccaac gtcctgactg gtataatgag
     1981 ccagttctta aaatcgcata aggtacataa ggtaattcac aatgattaaa gttgaaatta
     2041 aaccatctca agcccaattt actactcgtt ctggtgtttc tcgtcagggc aagccttatt
     2101 cactgaatga gcagctttgt tacgttgatt tgggtaatga atatccggtt cttgtcaaga
     2161 ttactcttga tgaaggtcag ccagcctatg cgcctggtct gtacaccgtt catctgtcct
     2221 ctttcaaagt tggtcagttc ggttccctta tgattgaccg tctgcgcctc gttccggcta
     2281 agtaaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     2341 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     2401 tttctgcgtt tata
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