BBa_M31538
type: ComponentDefinition
persistent identity: BBa M31538
display id: BBa_M31538
version: 1
was generated by: iGEM to SBOL conversion
description: This is the trio of genes in M13K07 responsible for Genome Amplification
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: Though the genes exist in nature in M13K07, they do not yet exist in this separate form.
owned by: james
owned by: myers
mutable description: This is the trio of genes in M13K07 responsible for Genome Amplification. In an effort to achieve more comprehensive abstractions, genes can be grouped into families depending upon their function.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1380
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This abstraction is useful for analysis of complex functions, but there are still interactions at the base pair level that need to be resolved before it can be implemented.
star: false
creator: Matt Gethers
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M31533
component: BBa_B0015
component: BBa_M31532
component: BBa_M31531
sequence annotation: BBa_M31531
sequence annotation: BBa_B0015
sequence annotation: BBa_M31532
sequence annotation: BBa_M31533
sequence: https://synbiohub.org/public/igem/BBa_M31538_sequence/1

BBa_M31533
type: Component
persistent identity: component2235182
display id: component2235182
version: 1
top level: BBa_M31538
definition: BBa_M31533
access: public

BBa_B0015
type: Component
persistent identity: component2235178
display id: component2235178
version: 1
top level: BBa_M31538
definition: BBa_B0015
access: public

BBa_M31532
type: Component
persistent identity: component2235171
display id: component2235171
version: 1
top level: BBa_M31538
definition: BBa_M31532
access: public

BBa_B0015
type: Component
persistent identity: component2235167
display id: component2235167
version: 1
top level: BBa_M31538
definition: BBa_B0015
access: public

BBa_B0015
type: Component
persistent identity: component2235189
display id: component2235189
version: 1
top level: BBa_M31538
definition: BBa_B0015
access: public

BBa_M31531
type: Component
persistent identity: component2235160
display id: component2235160
version: 1
top level: BBa_M31538
definition: BBa_M31531
access: public

BBa_M31531
type: SequenceAnnotation
persistent identity: annotation2235160
display id: annotation2235160
version: 1
top level: BBa_M31538
location: https://synbiohub.org/public/igem/BBa_M31538/annotation2235160/range2235160/1
component: BBa_M31531

https://synbiohub.org/public/igem/BBa_M31538/annotation2235160/range2235160/1
type: Range
persistent identity: range2235160
display id: range2235160
version: 1
top level: BBa_M31538
direction: direction/reverse
start: 1
end: 1297
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2235167
display id: annotation2235167
version: 1
top level: BBa_M31538
location: https://synbiohub.org/public/igem/BBa_M31538/annotation2235167/range2235167/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M31538/annotation2235167/range2235167/1
type: Range
persistent identity: range2235167
display id: range2235167
version: 1
top level: BBa_M31538
direction: direction/reverse
start: 1298
end: 1426
orientation: inline

BBa_M31532
type: SequenceAnnotation
persistent identity: annotation2235171
display id: annotation2235171
version: 1
top level: BBa_M31538
location: https://synbiohub.org/public/igem/BBa_M31538/annotation2235171/range2235171/1
component: BBa_M31532

https://synbiohub.org/public/igem/BBa_M31538/annotation2235171/range2235171/1
type: Range
persistent identity: range2235171
display id: range2235171
version: 1
top level: BBa_M31538
direction: direction/reverse
start: 1427
end: 1826
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2235178
display id: annotation2235178
version: 1
top level: BBa_M31538
location: https://synbiohub.org/public/igem/BBa_M31538/annotation2235178/range2235178/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M31538/annotation2235178/range2235178/1
type: Range
persistent identity: range2235178
display id: range2235178
version: 1
top level: BBa_M31538
direction: direction/reverse
start: 1827
end: 1955
orientation: inline

BBa_M31533
type: SequenceAnnotation
persistent identity: annotation2235182
display id: annotation2235182
version: 1
top level: BBa_M31538
location: https://synbiohub.org/public/igem/BBa_M31538/annotation2235182/range2235182/1
component: BBa_M31533

https://synbiohub.org/public/igem/BBa_M31538/annotation2235182/range2235182/1
type: Range
persistent identity: range2235182
display id: range2235182
version: 1
top level: BBa_M31538
direction: direction/reverse
start: 1956
end: 2285
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2235189
display id: annotation2235189
version: 1
top level: BBa_M31538
location: https://synbiohub.org/public/igem/BBa_M31538/annotation2235189/range2235189/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M31538/annotation2235189/range2235189/1
type: Range
persistent identity: range2235189
display id: range2235189
version: 1
top level: BBa_M31538
direction: direction/reverse
start: 2286
end: 2414
orientation: inline

BBa_M31531
type: ComponentDefinition
persistent identity: BBa M31531
display id: BBa_M31531
version: 1
was generated by: iGEM to SBOL conversion
description: This is gene II of M13K07 (Promoter, RBS, and ORF)
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: Though the gene and its regulatory sequences exist in nature in M13K07, they do not yet exist in this separate form.
owned by: james
owned by: myers
mutable description: When designing a phage, it is much more practical and effective to think of DNA in functional units than strings of regulartory and coding regions. This part combines the transcriptional and translational regions with the ORF to form a functional (where function is defined as the output of the gene).
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1380
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: When removed from it's place in the genome that has been determined by evolution, we can't be sure the change will be tolerated. There may be cross-talk between copied and separated elements with significant homology. Because we have removed and maintained entire portions of genes in the formation of separate genomic entities, there is also the possibility of expression of these 'partial' genes. I will address this by placing transciptional terminators and hair pins bewteen genes.
star: false
creator: Matt Gethers
type: DnaRegion
role: partType/DNA
role: SO:0000110
component: BBa_M13002
component: BBa_M13502
component: BBa_M13102
sequence annotation: BBa_M13102
sequence annotation: BBa_M13502
sequence annotation: BBa_M13002
sequence: https://synbiohub.org/public/igem/BBa_M31531_sequence/1

BBa_M13002
type: Component
persistent identity: component1917862
display id: component1917862
version: 1
top level: BBa_M31531
definition: BBa_M13002
access: public

BBa_M13502
type: Component
persistent identity: component1917861
display id: component1917861
version: 1
top level: BBa_M31531
definition: BBa_M13502
access: public

BBa_M13102
type: Component
persistent identity: component1917860
display id: component1917860
version: 1
top level: BBa_M31531
definition: BBa_M13102
access: public

BBa_M13102
type: SequenceAnnotation
persistent identity: annotation1917860
display id: annotation1917860
version: 1
top level: BBa_M31531
location: https://synbiohub.org/public/igem/BBa_M31531/annotation1917860/range1917860/1
component: BBa_M13102

https://synbiohub.org/public/igem/BBa_M31531/annotation1917860/range1917860/1
type: Range
persistent identity: range1917860
display id: range1917860
version: 1
top level: BBa_M31531
direction: direction/reverse
start: 1
end: 48
orientation: inline

BBa_M13502
type: SequenceAnnotation
persistent identity: annotation1917861
display id: annotation1917861
version: 1
top level: BBa_M31531
location: https://synbiohub.org/public/igem/BBa_M31531/annotation1917861/range1917861/1
component: BBa_M13502

https://synbiohub.org/public/igem/BBa_M31531/annotation1917861/range1917861/1
type: Range
persistent identity: range1917861
display id: range1917861
version: 1
top level: BBa_M31531
direction: direction/reverse
start: 49
end: 64
orientation: inline

BBa_M13002
type: SequenceAnnotation
persistent identity: annotation1917862
display id: annotation1917862
version: 1
top level: BBa_M31531
location: https://synbiohub.org/public/igem/BBa_M31531/annotation1917862/range1917862/1
component: BBa_M13002

https://synbiohub.org/public/igem/BBa_M31531/annotation1917862/range1917862/1
type: Range
persistent identity: range1917862
display id: range1917862
version: 1
top level: BBa_M31531
direction: direction/reverse
start: 65
end: 1297
orientation: inline

BBa_M31532
type: ComponentDefinition
persistent identity: BBa M31532
display id: BBa_M31532
version: 1
was generated by: iGEM to SBOL conversion
description: This is gene X of M13K07 (Promoter, RBS, and ORF)
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: Though the gene and its regulatory sequences exist in nature in M13K07, they do not yet exist in this separate form.
owned by: james
owned by: myers
mutable description: When designing a phage, it is much more practical and effective to think of DNA in functional units than strings of regulatory and coding regions. This part combines the transcriptional and translational regions with the ORF to form a functional (where function is defined as the output of the gene).
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1380
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: When removed from its place in the genome that has been determined by evolution, we can't be sure the change will be tolerated. There may be cross-talk between copied and separated elements with significant homology. Because we have removed and maintained entire portions of genes in the formation of separate genomic entities, there is also the possibility of expression of these 'partial' genes. I will address this by placing transcriptional terminators and hair pins between genes.
star: false
creator: Matt Gethers
type: DnaRegion
role: partType/DNA
role: SO:0000110
component: BBa_M13010
component: BBa_M13510
component: BBa_M13110
sequence annotation: BBa_M13110
sequence annotation: BBa_M13510
sequence annotation: BBa_M13010
sequence: https://synbiohub.org/public/igem/BBa_M31532_sequence/1

BBa_M13010
type: Component
persistent identity: component1917904
display id: component1917904
version: 1
top level: BBa_M31532
definition: BBa_M13010
access: public

BBa_M13510
type: Component
persistent identity: component1917903
display id: component1917903
version: 1
top level: BBa_M31532
definition: BBa_M13510
access: public

BBa_M13110
type: Component
persistent identity: component1917902
display id: component1917902
version: 1
top level: BBa_M31532
definition: BBa_M13110
access: public

BBa_M13110
type: SequenceAnnotation
persistent identity: annotation1917902
display id: annotation1917902
version: 1
top level: BBa_M31532
location: https://synbiohub.org/public/igem/BBa_M31532/annotation1917902/range1917902/1
component: BBa_M13110

https://synbiohub.org/public/igem/BBa_M31532/annotation1917902/range1917902/1
type: Range
persistent identity: range1917902
display id: range1917902
version: 1
top level: BBa_M31532
direction: direction/reverse
start: 1
end: 48
orientation: inline

BBa_M13510
type: SequenceAnnotation
persistent identity: annotation1917903
display id: annotation1917903
version: 1
top level: BBa_M31532
location: https://synbiohub.org/public/igem/BBa_M31532/annotation1917903/range1917903/1
component: BBa_M13510

https://synbiohub.org/public/igem/BBa_M31532/annotation1917903/range1917903/1
type: Range
persistent identity: range1917903
display id: range1917903
version: 1
top level: BBa_M31532
direction: direction/reverse
start: 49
end: 64
orientation: inline

BBa_M13010
type: SequenceAnnotation
persistent identity: annotation1917904
display id: annotation1917904
version: 1
top level: BBa_M31532
location: https://synbiohub.org/public/igem/BBa_M31532/annotation1917904/range1917904/1
component: BBa_M13010

https://synbiohub.org/public/igem/BBa_M31532/annotation1917904/range1917904/1
type: Range
persistent identity: range1917904
display id: range1917904
version: 1
top level: BBa_M31532
direction: direction/reverse
start: 65
end: 400
orientation: inline

BBa_M31533
type: ComponentDefinition
persistent identity: BBa M31533
display id: BBa_M31533
version: 1
was generated by: iGEM to SBOL conversion
description: This is gene V of M13K07 (Promoter, RBS, and ORF)
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: Though the gene and its regulatory sequences exist in nature in M13K07, they do not yet exist in this separate form.
owned by: james
owned by: myers
mutable description: When designing a phage, it is much more practical and effective to think of DNA in functional units than strings of regulatory and coding regions. This part combines the transcriptional and translational regions with the ORF to form a functional (where function is defined as the output of the gene).
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1380
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: When removed from its place in the genome that has been determined by evolution, we can't be sure the change will be tolerated. There may be cross-talk between copied and separated elements with significant homology. Because we have removed and maintained entire portions of genes in the formation of separate genomic entities, there is also the possibility of expression of these 'partial' genes. I will address this by placing transcriptional terminators and hair pins between genes.
star: false
creator: Matt Gethers
type: DnaRegion
role: partType/DNA
role: SO:0000110
component: BBa_M13105
component: BBa_M13005
component: BBa_M13505
sequence annotation: BBa_M13105
sequence annotation: BBa_M13505
sequence annotation: BBa_M13005
sequence: https://synbiohub.org/public/igem/BBa_M31533_sequence/1

BBa_M13105
type: Component
persistent identity: component1917912
display id: component1917912
version: 1
top level: BBa_M31533
definition: BBa_M13105
access: public

BBa_M13005
type: Component
persistent identity: component1917914
display id: component1917914
version: 1
top level: BBa_M31533
definition: BBa_M13005
access: public

BBa_M13505
type: Component
persistent identity: component1917913
display id: component1917913
version: 1
top level: BBa_M31533
definition: BBa_M13505
access: public

BBa_M13105
type: SequenceAnnotation
persistent identity: annotation1917912
display id: annotation1917912
version: 1
top level: BBa_M31533
location: https://synbiohub.org/public/igem/BBa_M31533/annotation1917912/range1917912/1
component: BBa_M13105

https://synbiohub.org/public/igem/BBa_M31533/annotation1917912/range1917912/1
type: Range
persistent identity: range1917912
display id: range1917912
version: 1
top level: BBa_M31533
direction: direction/reverse
start: 1
end: 50
orientation: inline

BBa_M13505
type: SequenceAnnotation
persistent identity: annotation1917913
display id: annotation1917913
version: 1
top level: BBa_M31533
location: https://synbiohub.org/public/igem/BBa_M31533/annotation1917913/range1917913/1
component: BBa_M13505

https://synbiohub.org/public/igem/BBa_M31533/annotation1917913/range1917913/1
type: Range
persistent identity: range1917913
display id: range1917913
version: 1
top level: BBa_M31533
direction: direction/reverse
start: 51
end: 66
orientation: inline

BBa_M13005
type: SequenceAnnotation
persistent identity: annotation1917914
display id: annotation1917914
version: 1
top level: BBa_M31533
location: https://synbiohub.org/public/igem/BBa_M31533/annotation1917914/range1917914/1
component: BBa_M13005

https://synbiohub.org/public/igem/BBa_M31533/annotation1917914/range1917914/1
type: Range
persistent identity: range1917914
display id: range1917914
version: 1
top level: BBa_M31533
direction: direction/reverse
start: 67
end: 330
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_M13002
type: ComponentDefinition
persistent identity: BBa M13002
display id: BBa_M13002
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene II
created: 2006-12-13T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome. It encodes a protein that is important for phage DNA replication, initiating ssDNA synthesis of the (+) strand by nicking the RF near the M13 replication origin. p10 is encoded within gII thanks to a translation re-initiation event.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: need to unstuff gX from within coding sequence. RBS for downstream gV overlaps the gII stop codon.
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M13002_sequence/1

BBa_M13502
type: ComponentDefinition
persistent identity: BBa M13502
display id: BBa_M13502
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene II RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene II message (BBa_M13002). The part aligns with base pairs 8252-8267 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13502_sequence/1

BBa_M13510
type: ComponentDefinition
persistent identity: BBa M13510
display id: BBa_M13510
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene X RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene X message (BBa_M13010). The part aligns with base pairs 480-495 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13510_sequence/1

BBa_M13102
type: ComponentDefinition
persistent identity: BBa M13102
display id: BBa_M13102
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene II promoter
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome. It directs transcription of M13 gene II (BBa_M13502, BBa_M13002). Promoter identified in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_M13102_sequence/1

BBa_M13110
type: ComponentDefinition
persistent identity: BBa M13110
display id: BBa_M13110
version: 1
was generated by: iGEM to SBOL conversion
description: M13110
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 381-428 in M13K07. It directs transcription of M13 gene X (BBa_M13510, BBa_M13010). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_M13110_sequence/1

BBa_M13010
type: ComponentDefinition
persistent identity: BBa M13010
display id: BBa_M13010
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene X
created: 2006-12-05T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome. It encodes a protein that is important for phage maturation. The protein remains in the host cytoplasm to interact with the product of gI and the OM pore (product of gIV) through which the phage ssDNA is threaded as the phage matures. GeneX protein is identical to the C-terminal portion of the gIprotein since gXprotien is normally encoded through an internal initiation of translation from the gI transcript.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: need to unstuff from inside gI
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M13010_sequence/1

BBa_M13005
type: ComponentDefinition
persistent identity: BBa M13005
display id: BBa_M13005
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene V
created: 2006-12-11T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome. It encodes a single stranded DNA binding protein that is important for phage maturation.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: RBS for gene V overlaps stop codon for upstream phage genes II and X.
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M13005_sequence/1

BBa_M13505
type: ComponentDefinition
persistent identity: BBa M13505
display id: BBa_M13505
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene V RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene V message (BBa_M13005). The part aligns with base pairs 827-842 in M13K07 genome. It was identified as the RBS for the gene V message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13505_sequence/1

BBa_M13105
type: ComponentDefinition
persistent identity: BBa M13105
display id: BBa_M13105
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene V promoter
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 786-835 in M13K07. It directs transcription of M13 gene V (BBa_M13505, BBa_M13005). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13105_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_M31538_sequence/1
type: Sequence
persistent identity: BBa M31538 sequence
display id: BBa_M31538_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tattaacgtttacaatttaaatatttgcttatacaatcttcctgttttatcaaccggggtacatatgattgacatgctagttttacgattaccgttcatcgattctcttgtttgctccagactctcaggcaatgacctgatagcctttgtagacctctcaaaaatagctaccctctccggcatgaatttatcagctagaacggttgaatatcatattgatggtgatttgactgtctccggcctttctcacccttttgaatctttacctacacattactcaggcattgcatttaaaatatatgagggttctaaaaatttttatccttgcgttgaaataaaggcttctcccgcaaaagtattacagggtcataatgtttttggtacaaccgatttagctttatgctctgaggctttattgcttaattttgctaattctttgccttgcctgtatgatttattggatgttaacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataccaacgtcctgactggtataatgagccagttcttaaaatcgcata…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31531_sequence/1
type: Sequence
persistent identity: BBa M31531 sequence
display id: BBa_M31531_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tattaacgtttacaatttaaatatttgcttatacaatcttcctgttttatcaaccggggtacatatgattgacatgctagttttacgattaccgttcatcgattctcttgtttgctccagactctcaggcaatgacctgatagcctttgtagacctctcaaaaatagctaccctctccggcatgaatttatcagctagaacggttgaatatcatattgatggtgatttgactgtctccggcctttctcacccttttgaatctttacctacacattactcaggcattgcatttaaaatatatgagggttctaaaaatttttatccttgcgttgaaataaaggcttctcccgcaaaagtattacagggtcataatgtttttggtacaaccgatttagctttatgctctgaggctttattgcttaattttgctaattctttgccttgcctgtatgatttattggatgttaacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31532_sequence/1
type: Sequence
persistent identity: BBa M31532 sequence
display id: BBa_M31532_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctttttgatgcaatccgctttgcttctgactataatagtcagggtaaatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31533_sequence/1
type: Sequence
persistent identity: BBa M31533 sequence
display id: BBa_M31533_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtacataaggtaattcacaatgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgttccggctaagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13002_sequence/1
type: Sequence
persistent identity: BBa M13002 sequence
display id: BBa_M13002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattgacatgctagttttacgattaccgttcatcgattctcttgtttgctccagactctcaggcaatgacctgatagcctttgtagacctctcaaaaatagctaccctctccggcatgaatttatcagctagaacggttgaatatcatattgatggtgatttgactgtctccggcctttctcacccttttgaatctttacctacacattactcaggcattgcatttaaaatatatgagggttctaaaaatttttatccttgcgttgaaataaaggcttctcccgcaaaagtattacagggtcataatgtttttggtacaaccgatttagctttatgctctgaggctttattgcttaattttgctaattctttgccttgcctgtatgatttattggatgttaacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13502_sequence/1
type: Sequence
persistent identity: BBa M13502 sequence
display id: BBa_M13502_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcaaccggggtacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13510_sequence/1
type: Sequence
persistent identity: BBa M13510 sequence
display id: BBa_M13510_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atttgagggggattca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13102_sequence/1
type: Sequence
persistent identity: BBa M13102 sequence
display id: BBa_M13102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tattaacgtttacaatttaaatatttgcttatacaatcttcctgtttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13110_sequence/1
type: Sequence
persistent identity: BBa M13110 sequence
display id: BBa_M13110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctttttgatgcaatccgctttgcttctgactataatagtcagggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13010_sequence/1
type: Sequence
persistent identity: BBa M13010 sequence
display id: BBa_M13010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13005_sequence/1
type: Sequence
persistent identity: BBa M13005 sequence
display id: BBa_M13005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgttccggctaagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13505_sequence/1
type: Sequence
persistent identity: BBa M13505 sequence
display id: BBa_M13505_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cataaggtaattcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13105_sequence/1
type: Sequence
persistent identity: BBa M13105 sequence
display id: BBa_M13105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z