BBa_M31540
type: ComponentDefinition
persistent identity: BBa M31540
display id: BBa_M31540
version: 1
was generated by: iGEM to SBOL conversion
description: Four Proteins involved in the production of the M13K07 phage coat.
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: Though the genes exist in nature in M13K07, they do not yet exist in this separate form.
owned by: james
owned by: myers
mutable description: Four Proteins involved in the production of the M13K07 phage coat.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1380
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This abstraction is useful for analysis of complex functions, but there are still interactions at the base pair level that need to be resolved before it can be implemented.
star: false
creator: Matt Gethers
type: DnaRegion
role: partType/DNA
role: SO:0000110
component: BBa_M31535
component: BBa_B0015
component: BBa_M31534
component: BBa_M31537
component: BBa_M31536
sequence annotation: BBa_M31534
sequence annotation: BBa_B0015
sequence annotation: BBa_M31535
sequence annotation: BBa_M31536
sequence annotation: BBa_M31537
sequence: https://synbiohub.org/public/igem/BBa_M31540_sequence/1

BBa_M31535
type: Component
persistent identity: component2235810
display id: component2235810
version: 1
top level: BBa_M31540
definition: BBa_M31535
access: public

BBa_B0015
type: Component
persistent identity: component2235807
display id: component2235807
version: 1
top level: BBa_M31540
definition: BBa_B0015
access: public

BBa_M31534
type: Component
persistent identity: component2235800
display id: component2235800
version: 1
top level: BBa_M31540
definition: BBa_M31534
access: public

BBa_M31537
type: Component
persistent identity: component2235832
display id: component2235832
version: 1
top level: BBa_M31540
definition: BBa_M31537
access: public

BBa_B0015
type: Component
persistent identity: component2235828
display id: component2235828
version: 1
top level: BBa_M31540
definition: BBa_B0015
access: public

BBa_M31536
type: Component
persistent identity: component2235821
display id: component2235821
version: 1
top level: BBa_M31540
definition: BBa_M31536
access: public

BBa_B0015
type: Component
persistent identity: component2235817
display id: component2235817
version: 1
top level: BBa_M31540
definition: BBa_B0015
access: public

BBa_M31534
type: SequenceAnnotation
persistent identity: annotation2235800
display id: annotation2235800
version: 1
top level: BBa_M31540
location: https://synbiohub.org/public/igem/BBa_M31540/annotation2235800/range2235800/1
component: BBa_M31534

https://synbiohub.org/public/igem/BBa_M31540/annotation2235800/range2235800/1
type: Range
persistent identity: range2235800
display id: range2235800
version: 1
top level: BBa_M31540
direction: direction/reverse
start: 1
end: 124
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2235807
display id: annotation2235807
version: 1
top level: BBa_M31540
location: https://synbiohub.org/public/igem/BBa_M31540/annotation2235807/range2235807/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M31540/annotation2235807/range2235807/1
type: Range
persistent identity: range2235807
display id: range2235807
version: 1
top level: BBa_M31540
direction: direction/reverse
start: 125
end: 253
orientation: inline

BBa_M31535
type: SequenceAnnotation
persistent identity: annotation2235810
display id: annotation2235810
version: 1
top level: BBa_M31540
location: https://synbiohub.org/public/igem/BBa_M31540/annotation2235810/range2235810/1
component: BBa_M31535

https://synbiohub.org/public/igem/BBa_M31540/annotation2235810/range2235810/1
type: Range
persistent identity: range2235810
display id: range2235810
version: 1
top level: BBa_M31540
direction: direction/reverse
start: 254
end: 368
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2235817
display id: annotation2235817
version: 1
top level: BBa_M31540
location: https://synbiohub.org/public/igem/BBa_M31540/annotation2235817/range2235817/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M31540/annotation2235817/range2235817/1
type: Range
persistent identity: range2235817
display id: range2235817
version: 1
top level: BBa_M31540
direction: direction/reverse
start: 369
end: 497
orientation: inline

BBa_M31536
type: SequenceAnnotation
persistent identity: annotation2235821
display id: annotation2235821
version: 1
top level: BBa_M31540
location: https://synbiohub.org/public/igem/BBa_M31540/annotation2235821/range2235821/1
component: BBa_M31536

https://synbiohub.org/public/igem/BBa_M31540/annotation2235821/range2235821/1
type: Range
persistent identity: range2235821
display id: range2235821
version: 1
top level: BBa_M31540
direction: direction/reverse
start: 498
end: 782
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2235828
display id: annotation2235828
version: 1
top level: BBa_M31540
location: https://synbiohub.org/public/igem/BBa_M31540/annotation2235828/range2235828/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M31540/annotation2235828/range2235828/1
type: Range
persistent identity: range2235828
display id: range2235828
version: 1
top level: BBa_M31540
direction: direction/reverse
start: 783
end: 911
orientation: inline

BBa_M31537
type: SequenceAnnotation
persistent identity: annotation2235832
display id: annotation2235832
version: 1
top level: BBa_M31540
location: https://synbiohub.org/public/igem/BBa_M31540/annotation2235832/range2235832/1
component: BBa_M31537

https://synbiohub.org/public/igem/BBa_M31540/annotation2235832/range2235832/1
type: Range
persistent identity: range2235832
display id: range2235832
version: 1
top level: BBa_M31540
direction: direction/reverse
start: 912
end: 2250
orientation: inline

BBa_M31534
type: ComponentDefinition
persistent identity: BBa M31534
display id: BBa_M31534
version: 1
was generated by: iGEM to SBOL conversion
description: This is gene VII of M13K07 (RBS, and ORF)
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: Though the gene and its regulatory sequences exist in nature in M13K07, they do not yet exist in this separate form.
owned by: james
owned by: myers
mutable description: When designing a phage, it is much more practical and effective to think of DNA in functional units than strings of regulatory and coding regions. This part combines the transcriptional and translational regions with the ORF to form a functional (where function is defined as the output of the gene).
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1380
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: When removed from its place in the genome that has been determined by evolution, we can't be sure the change will be tolerated. There may be cross-talk between copied and separated elements with significant homology. Because we have removed and maintained entire portions of genes in the formation of separate genomic entities, there is also the possibility of expression of these 'partial' genes. I will address this by placing transcriptional terminators and hair pins between genes.
star: false
creator: Matt Gethers
type: DnaRegion
role: SO:0000110
role: partType/DNA
component: BBa_M13007
component: BBa_M13507
sequence annotation: BBa_M13507
sequence annotation: BBa_M13007
sequence: https://synbiohub.org/public/igem/BBa_M31534_sequence/1

BBa_M13007
type: Component
persistent identity: component1917916
display id: component1917916
version: 1
top level: BBa_M31534
definition: BBa_M13007
access: public

BBa_M13507
type: Component
persistent identity: component1917915
display id: component1917915
version: 1
top level: BBa_M31534
definition: BBa_M13507
access: public

BBa_M13507
type: SequenceAnnotation
persistent identity: annotation1917915
display id: annotation1917915
version: 1
top level: BBa_M31534
location: https://synbiohub.org/public/igem/BBa_M31534/annotation1917915/range1917915/1
component: BBa_M13507

https://synbiohub.org/public/igem/BBa_M31534/annotation1917915/range1917915/1
type: Range
persistent identity: range1917915
display id: range1917915
version: 1
top level: BBa_M31534
direction: direction/reverse
start: 1
end: 16
orientation: inline

BBa_M13007
type: SequenceAnnotation
persistent identity: annotation1917916
display id: annotation1917916
version: 1
top level: BBa_M31534
location: https://synbiohub.org/public/igem/BBa_M31534/annotation1917916/range1917916/1
component: BBa_M13007

https://synbiohub.org/public/igem/BBa_M31534/annotation1917916/range1917916/1
type: Range
persistent identity: range1917916
display id: range1917916
version: 1
top level: BBa_M31534
direction: direction/reverse
start: 23
end: 124
orientation: inline

BBa_M31535
type: ComponentDefinition
persistent identity: BBa M31535
display id: BBa_M31535
version: 1
was generated by: iGEM to SBOL conversion
description: This is gene IX of M13K07 (RBS, and ORF)
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: Though the gene and its regulatory sequences exist in nature in M13K07, they do not yet exist in this separate form.
owned by: james
owned by: myers
mutable description: When designing a phage, it is much more practical and effective to think of DNA in functional units than strings of regulatory and coding regions. This part combines the transcriptional and translational regions with the ORF to form a functional (where function is defined as the output of the gene).
discontinued: false
dominant: true
group u list: _102_
m user id: 0
owner id: 1380
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: When removed from its place in the genome that has been determined by evolution, we can't be sure the change will be tolerated. There may be cross-talk between copied and separated elements with significant homology. Because we have removed and maintained entire portions of genes in the formation of separate genomic entities, there is also the possibility of expression of these 'partial' genes. I will address this by placing transcriptional terminators and hair pins between genes.
star: false
creator: Matt Gethers
type: DnaRegion
role: partType/DNA
role: SO:0000110
component: BBa_M13009
component: BBa_M13509
sequence annotation: BBa_M13509
sequence annotation: BBa_M13009
sequence: https://synbiohub.org/public/igem/BBa_M31535_sequence/1

BBa_M13009
type: Component
persistent identity: component1917942
display id: component1917942
version: 1
top level: BBa_M31535
definition: BBa_M13009
access: public

BBa_M13509
type: Component
persistent identity: component1917941
display id: component1917941
version: 1
top level: BBa_M31535
definition: BBa_M13509
access: public

BBa_M13509
type: SequenceAnnotation
persistent identity: annotation1917941
display id: annotation1917941
version: 1
top level: BBa_M31535
location: https://synbiohub.org/public/igem/BBa_M31535/annotation1917941/range1917941/1
component: BBa_M13509

https://synbiohub.org/public/igem/BBa_M31535/annotation1917941/range1917941/1
type: Range
persistent identity: range1917941
display id: range1917941
version: 1
top level: BBa_M31535
direction: direction/reverse
start: 1
end: 16
orientation: inline

BBa_M13009
type: SequenceAnnotation
persistent identity: annotation1917942
display id: annotation1917942
version: 1
top level: BBa_M31535
location: https://synbiohub.org/public/igem/BBa_M31535/annotation1917942/range1917942/1
component: BBa_M13009

https://synbiohub.org/public/igem/BBa_M31535/annotation1917942/range1917942/1
type: Range
persistent identity: range1917942
display id: range1917942
version: 1
top level: BBa_M31535
direction: direction/reverse
start: 17
end: 115
orientation: inline

BBa_M31536
type: ComponentDefinition
persistent identity: BBa M31536
display id: BBa_M31536
version: 1
was generated by: iGEM to SBOL conversion
description: This is gene VIII of M13K07 (Promoter, RBS, and ORF)
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: Though the gene and its regulatory sequences exist in nature in M13K07, they do not yet exist in this separate form.
owned by: james
owned by: myers
mutable description: When designing a phage, it is much more practical and effective to think of DNA in functional units than strings of regulatory and coding regions. This part combines the transcriptional and translational regions with the ORF to form a functional (where function is defined as the output of the gene).
discontinued: false
dominant: true
group u list: _102_
m user id: 0
owner id: 1380
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: When removed from its place in the genome that has been determined by evolution, we can't be sure the change will be tolerated. There may be cross-talk between copied and separated elements with significant homology. Because we have removed and maintained entire portions of genes in the formation of separate genomic entities, there is also the possibility of expression of these 'partial' genes. I will address this by placing transcriptional terminators and hair pins between genes.
star: false
creator: Matt Gethers
type: DnaRegion
role: SO:0000110
role: partType/DNA
component: BBa_M13008
component: BBa_M13508
component: BBa_M13108
sequence annotation: BBa_M13108
sequence annotation: BBa_M13508
sequence annotation: BBa_M13008
sequence: https://synbiohub.org/public/igem/BBa_M31536_sequence/1

BBa_M13008
type: Component
persistent identity: component1917921
display id: component1917921
version: 1
top level: BBa_M31536
definition: BBa_M13008
access: public

BBa_M13508
type: Component
persistent identity: component1917920
display id: component1917920
version: 1
top level: BBa_M31536
definition: BBa_M13508
access: public

BBa_M13108
type: Component
persistent identity: component1917919
display id: component1917919
version: 1
top level: BBa_M31536
definition: BBa_M13108
access: public

BBa_M13108
type: SequenceAnnotation
persistent identity: annotation1917919
display id: annotation1917919
version: 1
top level: BBa_M31536
location: https://synbiohub.org/public/igem/BBa_M31536/annotation1917919/range1917919/1
component: BBa_M13108

https://synbiohub.org/public/igem/BBa_M31536/annotation1917919/range1917919/1
type: Range
persistent identity: range1917919
display id: range1917919
version: 1
top level: BBa_M31536
direction: direction/reverse
start: 1
end: 47
orientation: inline

BBa_M13508
type: SequenceAnnotation
persistent identity: annotation1917920
display id: annotation1917920
version: 1
top level: BBa_M31536
location: https://synbiohub.org/public/igem/BBa_M31536/annotation1917920/range1917920/1
component: BBa_M13508

https://synbiohub.org/public/igem/BBa_M31536/annotation1917920/range1917920/1
type: Range
persistent identity: range1917920
display id: range1917920
version: 1
top level: BBa_M31536
direction: direction/reverse
start: 48
end: 63
orientation: inline

BBa_M13008
type: SequenceAnnotation
persistent identity: annotation1917921
display id: annotation1917921
version: 1
top level: BBa_M31536
location: https://synbiohub.org/public/igem/BBa_M31536/annotation1917921/range1917921/1
component: BBa_M13008

https://synbiohub.org/public/igem/BBa_M31536/annotation1917921/range1917921/1
type: Range
persistent identity: range1917921
display id: range1917921
version: 1
top level: BBa_M31536
direction: direction/reverse
start: 64
end: 285
orientation: inline

BBa_M31537
type: ComponentDefinition
persistent identity: BBa M31537
display id: BBa_M31537
version: 1
was generated by: iGEM to SBOL conversion
description: This is gene III of M13K07 (Promoter, RBS, and ORF)
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: Though the gene and its regulatory sequences exist in nature in M13K07, they do not yet exist in this separate form.
owned by: james
owned by: myers
mutable description: When designing a phage, it is much more practical and effective to think of DNA in functional units than strings of regulatory and coding regions. This part combines the transcriptional and translational regions with the ORF to form a functional (where function is defined as the output of the gene).
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1380
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: When removed from its place in the genome that has been determined by evolution, we can't be sure the change will be tolerated. There may be cross-talk between copied and separated elements with significant homology. Because we have removed and maintained entire portions of genes in the formation of separate genomic entities, there is also the possibility of expression of these 'partial' genes. I will address this by placing transcriptional terminators and hair pins between genes.
star: false
creator: Matt Gethers
type: DnaRegion
role: SO:0000110
role: partType/DNA
component: BBa_M13103
component: BBa_M13003
component: BBa_M13503
sequence annotation: BBa_M13103
sequence annotation: BBa_M13503
sequence annotation: BBa_M13003
sequence: https://synbiohub.org/public/igem/BBa_M31537_sequence/1

BBa_M13103
type: Component
persistent identity: component1917938
display id: component1917938
version: 1
top level: BBa_M31537
definition: BBa_M13103
access: public

BBa_M13003
type: Component
persistent identity: component1917940
display id: component1917940
version: 1
top level: BBa_M31537
definition: BBa_M13003
access: public

BBa_M13503
type: Component
persistent identity: component1917939
display id: component1917939
version: 1
top level: BBa_M31537
definition: BBa_M13503
access: public

BBa_M13103
type: SequenceAnnotation
persistent identity: annotation1917938
display id: annotation1917938
version: 1
top level: BBa_M31537
location: https://synbiohub.org/public/igem/BBa_M31537/annotation1917938/range1917938/1
component: BBa_M13103

https://synbiohub.org/public/igem/BBa_M31537/annotation1917938/range1917938/1
type: Range
persistent identity: range1917938
display id: range1917938
version: 1
top level: BBa_M31537
direction: direction/reverse
start: 1
end: 48
orientation: inline

BBa_M13503
type: SequenceAnnotation
persistent identity: annotation1917939
display id: annotation1917939
version: 1
top level: BBa_M31537
location: https://synbiohub.org/public/igem/BBa_M31537/annotation1917939/range1917939/1
component: BBa_M13503

https://synbiohub.org/public/igem/BBa_M31537/annotation1917939/range1917939/1
type: Range
persistent identity: range1917939
display id: range1917939
version: 1
top level: BBa_M31537
direction: direction/reverse
start: 49
end: 64
orientation: inline

BBa_M13003
type: SequenceAnnotation
persistent identity: annotation1917940
display id: annotation1917940
version: 1
top level: BBa_M31537
location: https://synbiohub.org/public/igem/BBa_M31537/annotation1917940/range1917940/1
component: BBa_M13003

https://synbiohub.org/public/igem/BBa_M31537/annotation1917940/range1917940/1
type: Range
persistent identity: range1917940
display id: range1917940
version: 1
top level: BBa_M31537
direction: direction/reverse
start: 65
end: 1339
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_M13007
type: ComponentDefinition
persistent identity: BBa M13007
display id: BBa_M13007
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene VII
created: 2006-12-11T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome. It encodes a protein that is presented with p9 to initiate phage assembly at the blunt end of the phage coat. Approximately 5 copies of each protein are presented on mature phage. Without p9 or p7, no phage is produced. p7 is one of the smallest ribosomally translated proteins with described function. It is hydrophobic and the N-terminus is negatively charged, suggesting a C-terminus inside orientation.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: stop codon overlaps with p9 start codon in M13 genome.
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M13007_sequence/1

BBa_M13009
type: ComponentDefinition
persistent identity: BBa M13009
display id: BBa_M13009
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene IX
created: 2006-12-11T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome. It encodes a protein that is presented with p7 to initiate phage assembly at the blunt end of the phage coat. Approximately 5 copies of each protein are presented on mature phage. In the absence of p9 or p7, no phage is produced. p9 is one of the smallest ribosomally translated proteins with described function. N-terminal display is possible, and some positively charged residues are seen at the C-terminal end of the protein, suggesting a "C-terminus inside" orientation.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: start codon overlaps with stop codon of g7 upstream and stop codon overlaps with start codon of g8 downstream.
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_M13009_sequence/1

BBa_M13507
type: ComponentDefinition
persistent identity: BBa M13507
display id: BBa_M13507
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene VII RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VII message (BBa_M13007). The part aligns with base pairs 1092-1107 in M13K07 genome. It was identified as the RBS for gene VII message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13507_sequence/1

BBa_M13509
type: ComponentDefinition
persistent identity: BBa M13509
display id: BBa_M13509
version: 1
was generated by: iGEM to SBOL conversion
description: M13Ko7 gene IX RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene IX message (BBa_M13009). The part aligns with base pairs 1190-1205 in M13K07 genome. It was identified as the RBS for gene IX message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13509_sequence/1

BBa_M13008
type: ComponentDefinition
persistent identity: BBa M13008
display id: BBa_M13008
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene VIII
created: 2006-12-12T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome. It encodes the major coat protein, with approximately 2700 copies of the protein on each phage particle. The protein is first synthesized at a longer precursor protein that is clipped between the Alanines at positions 23 and 24. It is possible to silently add a Pst site to clone fusions upstream and in frame with Ala that begins mature protein. Small peptide-fusions are possible to the N-terminal portion of the mature p8 (phage display systems are built around this finding) but addition of more than 6 or 8 residues makes the phage pack poorly, leading to unproductive infections.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Many! The start codon for gene 8 overlaps with the stop codon for the upstream gene 9. Might be nice to include cloning sites for directed N-terminal fusions to mature form of protein. Might be nice to codon vary a different biobrick of gene VIII to allow for two genes expressed on single genome, inhibiting recombination in ssDNA.
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_M13008_sequence/1

BBa_M13003
type: ComponentDefinition
persistent identity: BBa M13003
display id: BBa_M13003
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene III
created: 2006-12-13T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome, encoding a minor coat protein. Approximately 5 copies of p3 are presented with 5 copies of p6 on the rounded end of the mature phage. In the absence of p3, the phage remains tethered to the bacterial host, elongating to 10 or 20 times the normal phage length and incorporating multiple ssDNA genomes within the coat. p3 (domain II) is also responsible for the initial interaction of the phage with the bacterial pili during infection. Peptide fusions, sometimes large, can be displayed on N-terminal end of p3, though highly charged fusions are difficult to isolate since they are not easily secreted by the host secretion apparatus. The p3 protein is first synthesized as a longer precursor protein that is 18 amino acids longer than the mature protein. The gene's translation initiates with a GTG codon, and changing this codon to an ATG increases expression of gene III to levels with negative effects on phage maturation.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The RBS for gene III is within the transcription termination sequence of the upstream gVIII, thus modifications to termination affect gIII initiation. Translation initiates with a GTG rather than a canonical ATG. There is a unique, natural BamHI site in domain II that may be useful for cloning, though it may prevent interaction of p3 with pilus.
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_M13003_sequence/1

BBa_M13508
type: ComponentDefinition
persistent identity: BBa M13508
display id: BBa_M13508
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene VIII RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VIII message (BBa_M13008). The part aligns with base pairs 1285-1300 in M13K07 genome. It was identified as the RBS for gene VIII message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13508_sequence/1

BBa_M13503
type: ComponentDefinition
persistent identity: BBa M13503
display id: BBa_M13503
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene III RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene III message (BBa_M13003). The part aligns with base pairs 1563-1578 in M13K07 genome. It was identified as the RBS for gene III message by Wezenbeek et al in Gene (1980) 11: 129-148 New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13503_sequence/1

BBa_M13108
type: ComponentDefinition
persistent identity: BBa M13108
display id: BBa_M13108
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene VIII promoter
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 1155-1201 in M13K07. It directs transcription of M13 gene VIII (BBa_M13508, BBa_M13008). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13108_sequence/1

BBa_M13103
type: ComponentDefinition
persistent identity: BBa M13103
display id: BBa_M13103
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene III promoter
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 1500-1547 in M13K07. It directs transcription of M13 gene III (BBa_M13503, BBa_M13003). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_M13103_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_M31540_sequence/1
type: Sequence
persistent identity: BBa M31540 sequence
display id: BBa_M31540_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttccggctaagtaactactagatggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatctccgttgtactttgtttcgcgcttggtataatcgctgggggtcaaagatgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatcgctgggggtcaaagatgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttaatggaaacttcctcatgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataaatctccgttgtactttgtttcgcgcttggtataatcgctgggggtctaatggaaacttcctcatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattcacctcgaaagcaagctgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataaattcacctcgaaagcaagctgataaaccgatacaattaaaggctccttttggagattttcaacgtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccgcctggtactgagcaaaaccccgctaatcctaatccttctcttgaggagtctcagcctcttaatactttcatgtttcagaataataggttccgaaataggcagggggcattaactgtttatacgggcactgttactcaaggcactgaccccgttaaaacttattaccagtacactcctgtatcatcaaaagccatgtatgacgcttactggaacggtaaattcagagactgcgctttccattctggctttaatgaggatccattcgtttgtgaatatcaaggccaatcgtctgacctgcctcaacctcctgtcaatgctggcggcggctctggtggtggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctctgagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacgctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaaacttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggccttgctaatggtaatggtgctactggtgattttgctggctctaattc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31534_sequence/1
type: Sequence
persistent identity: BBa M31534 sequence
display id: BBa_M31534_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttccggctaagtaactactagatggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatctccgttgtactttgtttcgcgcttggtataatcgctgggggtcaaagatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31535_sequence/1
type: Sequence
persistent identity: BBa M31535 sequence
display id: BBa_M31535_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcgctgggggtcaaagatgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttaatggaaacttcctcatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31536_sequence/1
type: Sequence
persistent identity: BBa M31536 sequence
display id: BBa_M31536_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatctccgttgtactttgtttcgcgcttggtataatcgctgggggtctaatggaaacttcctcatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattcacctcgaaagcaagctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31537_sequence/1
type: Sequence
persistent identity: BBa M31537 sequence
display id: BBa_M31537_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattcacctcgaaagcaagctgataaaccgatacaattaaaggctccttttggagattttcaacgtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccgcctggtactgagcaaaaccccgctaatcctaatccttctcttgaggagtctcagcctcttaatactttcatgtttcagaataataggttccgaaataggcagggggcattaactgtttatacgggcactgttactcaaggcactgaccccgttaaaacttattaccagtacactcctgtatcatcaaaagccatgtatgacgcttactggaacggtaaattcagagactgcgctttccattctggctttaatgaggatccattcgtttgtgaatatcaaggccaatcgtctgacctgcctcaacctcctgtcaatgctggcggcggctctggtggtggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctctgagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacgctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaaacttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggccttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcggtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatcggttgaatgtcgcccttttgtctttagcgctggtaaaccatatgaattttctattgattgtgacaaaataaacttattccgtggtgtctttgcgtttcttttatatgttgccacctttatgtatgtattttctacgtttgctaacatactgcgtaataaggagtcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13007_sequence/1
type: Sequence
persistent identity: BBa M13007 sequence
display id: BBa_M13007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatctccgttgtactttgtttcgcgcttggtataatcgctgggggtcaaagatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13009_sequence/1
type: Sequence
persistent identity: BBa M13009 sequence
display id: BBa_M13009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttaatggaaacttcctcatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13507_sequence/1
type: Sequence
persistent identity: BBa M13507 sequence
display id: BBa_M13507_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttccggctaagtaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13509_sequence/1
type: Sequence
persistent identity: BBa M13509 sequence
display id: BBa_M13509_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcgctgggggtcaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13008_sequence/1
type: Sequence
persistent identity: BBa M13008 sequence
display id: BBa_M13008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattcacctcgaaagcaagctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13003_sequence/1
type: Sequence
persistent identity: BBa M13003 sequence
display id: BBa_M13003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccgcctggtactgagcaaaaccccgctaatcctaatccttctcttgaggagtctcagcctcttaatactttcatgtttcagaataataggttccgaaataggcagggggcattaactgtttatacgggcactgttactcaaggcactgaccccgttaaaacttattaccagtacactcctgtatcatcaaaagccatgtatgacgcttactggaacggtaaattcagagactgcgctttccattctggctttaatgaggatccattcgtttgtgaatatcaaggccaatcgtctgacctgcctcaacctcctgtcaatgctggcggcggctctggtggtggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctctgagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacgctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaaacttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggccttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcggtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatcggttgaatgtcgcccttttgtctttagcgctggtaaaccatatgaattttctattgattgtgacaaaataaacttattccgtggtgtctttgcgtttcttttatatgttgccacctttatgtatgtattttctacgtttgctaacatactgcgtaataaggagtcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13508_sequence/1
type: Sequence
persistent identity: BBa M13508 sequence
display id: BBa_M13508_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatggaaacttcctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13503_sequence/1
type: Sequence
persistent identity: BBa M13503 sequence
display id: BBa_M13503_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttggagattttcaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13108_sequence/1
type: Sequence
persistent identity: BBa M13108 sequence
display id: BBa_M13108_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatctccgttgtactttgtttcgcgcttggtataatcgctgggggtc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13103_sequence/1
type: Sequence
persistent identity: BBa M13103 sequence
display id: BBa_M13103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattcacctcgaaagcaagctgataaaccgatacaattaaaggctcct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z