LOCUS BBa_M31541 4664 bp DNA linear 29-Nov-2024 DEFINITION This is the M13K07 genome refactored between the Hap1 and Bam1 Sites ACCESSION BBa_M31541 VERSION BBa_M31541.1 FEATURES Location/Qualifiers promoter 1..48 /label=BBa_M13102 RBS 49..64 /label=BBa_M13502 CDS 65..1297 /label=BBa_M13002 misc_feature 1..1297 /label=BBa_M31531 stem_loop 1309..1352 /label=stem_loop misc_feature 1298..1377 /label=BBa_B0010 terminator 1298..1377 /label=BBa_B0010 stem_loop 1393..1412 /label=T7 TE misc_feature 1419..1419 /label=stop polyA_site 1413..1426 /label=polya misc_feature 1386..1426 /label=BBa_B0012 terminator 1386..1426 /label=BBa_B0012 terminator 1298..1426 /label=BBa_B0015 promoter 1427..1474 /label=BBa_M13110 RBS 1475..1490 /label=BBa_M13510 CDS 1491..1826 /label=BBa_M13010 misc_feature 1427..1826 /label=BBa_M31532 stem_loop 1838..1881 /label=stem_loop misc_feature 1827..1906 /label=BBa_B0010 terminator 1827..1906 /label=BBa_B0010 stem_loop 1922..1941 /label=T7 TE misc_feature 1948..1948 /label=stop polyA_site 1942..1955 /label=polya misc_feature 1915..1955 /label=BBa_B0012 terminator 1915..1955 /label=BBa_B0012 terminator 1827..1955 /label=BBa_B0015 promoter 1956..2005 /label=BBa_M13105 RBS 2006..2021 /label=BBa_M13505 CDS 2022..2285 /label=BBa_M13005 misc_feature 1956..2285 /label=BBa_M31533 stem_loop 2297..2340 /label=stem_loop misc_feature 2286..2365 /label=BBa_B0010 terminator 2286..2365 /label=BBa_B0010 stem_loop 2381..2400 /label=T7 TE misc_feature 2407..2407 /label=stop polyA_site 2401..2414 /label=polya misc_feature 2374..2414 /label=BBa_B0012 terminator 2374..2414 /label=BBa_B0012 terminator 2286..2414 /label=BBa_B0015 misc_feature 1..2414 /label=BBa_M31538 RBS 2415..2430 /label=BBa_M13507 CDS 2437..2538 /label=BBa_M13007 misc_feature 2415..2538 /label=BBa_M31534 stem_loop 2550..2593 /label=stem_loop misc_feature 2539..2618 /label=BBa_B0010 terminator 2539..2618 /label=BBa_B0010 stem_loop 2634..2653 /label=T7 TE misc_feature 2660..2660 /label=stop polyA_site 2654..2667 /label=polya misc_feature 2627..2667 /label=BBa_B0012 terminator 2627..2667 /label=BBa_B0012 terminator 2539..2667 /label=BBa_B0015 RBS 2668..2683 /label=BBa_M13509 CDS 2684..2782 /label=BBa_M13009 misc_feature 2668..2782 /label=BBa_M31535 stem_loop 2794..2837 /label=stem_loop misc_feature 2783..2862 /label=BBa_B0010 terminator 2783..2862 /label=BBa_B0010 stem_loop 2878..2897 /label=T7 TE misc_feature 2904..2904 /label=stop polyA_site 2898..2911 /label=polya misc_feature 2871..2911 /label=BBa_B0012 terminator 2871..2911 /label=BBa_B0012 terminator 2783..2911 /label=BBa_B0015 promoter 2912..2958 /label=BBa_M13108 RBS 2959..2974 /label=BBa_M13508 CDS 2975..3196 /label=BBa_M13008 misc_feature 2912..3196 /label=BBa_M31536 stem_loop 3208..3251 /label=stem_loop misc_feature 3197..3276 /label=BBa_B0010 terminator 3197..3276 /label=BBa_B0010 stem_loop 3292..3311 /label=T7 TE misc_feature 3318..3318 /label=stop polyA_site 3312..3325 /label=polya misc_feature 3285..3325 /label=BBa_B0012 terminator 3285..3325 /label=BBa_B0012 terminator 3197..3325 /label=BBa_B0015 promoter 3326..3373 /label=BBa_M13103 RBS 3374..3389 /label=BBa_M13503 CDS 3390..4664 /label=BBa_M13003 misc_feature 3326..4664 /label=BBa_M31537 misc_feature 2415..4664 /label=BBa_M31540 ORIGIN 1 tattaacgtt tacaatttaa atatttgctt atacaatctt cctgttttat caaccggggt 61 acatatgatt gacatgctag ttttacgatt accgttcatc gattctcttg tttgctccag 121 actctcaggc aatgacctga tagcctttgt agacctctca aaaatagcta ccctctccgg 181 catgaattta tcagctagaa cggttgaata tcatattgat ggtgatttga ctgtctccgg 241 cctttctcac ccttttgaat ctttacctac acattactca ggcattgcat ttaaaatata 301 tgagggttct aaaaattttt atccttgcgt tgaaataaag gcttctcccg caaaagtatt 361 acagggtcat aatgtttttg gtacaaccga tttagcttta tgctctgagg ctttattgct 421 taattttgct aattctttgc cttgcctgta tgatttattg gatgttaacg ctactactat 481 tagtagaatt gatgccacct tttcagctcg cgccccaaat gaaaatatag ctaaacaggt 541 tattgaccat ttgcgaaatg tatctaatgg tcaaactaaa tctactcgtt cgcagaattg 601 ggaatcaact gttacatgga atgaaacttc cagacaccgt actttagttg catatttaaa 661 acatgttgag ctacagcacc agattcagca attaagctct aagccatccg caaaaatgac 721 ctcttatcaa aaggagcaat taaaggtact ctctaatcct gacctgttgg agtttgcttc 781 cggtctggtt cgctttgaag ctcgaattaa aacgcgatat ttgaagtctt tcgggcttcc 841 tcttaatctt tttgatgcaa tccgctttgc ttctgactat aatagtcagg gtaaagacct 901 gatttttgat ttatggtcat tctcgttttc tgaactgttt aaagcatttg agggggattc 961 aatgaatatt tatgacgatt ccgcagtatt ggacgctatc cagtctaaac attttactat 1021 taccccctct ggcaaaactt cttttgcaaa agcctctcgc tattttggtt tttatcgtcg 1081 tctggtaaac gagggttatg atagtgttgc tcttactatg cctcgtaatt ccttttggcg 1141 ttatgtatct gcattagttg aatgtggtat tcctaaatct caactgatga atctttctac 1201 ctgtaataat gttgttccgt tagttcgttt tattaacgta gatttttctt cccaacgtcc 1261 tgactggtat aatgagccag ttcttaaaat cgcataacca ggcatcaaat aaaacgaaag 1321 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 1381 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatctt tttgatgcaa 1441 tccgctttgc ttctgactat aatagtcagg gtaaatttga gggggattca atgaatattt 1501 atgacgattc cgcagtattg gacgctatcc agtctaaaca ttttactatt accccctctg 1561 gcaaaacttc ttttgcaaaa gcctctcgct attttggttt ttatcgtcgt ctggtaaacg 1621 agggttatga tagtgttgct cttactatgc ctcgtaattc cttttggcgt tatgtatctg 1681 cattagttga atgtggtatt cctaaatctc aactgatgaa tctttctacc tgtaataatg 1741 ttgttccgtt agttcgtttt attaacgtag atttttcttc ccaacgtcct gactggtata 1801 atgagccagt tcttaaaatc gcataaccag gcatcaaata aaacgaaagg ctcagtcgaa 1861 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 1921 tggctcacct tcgggtgggc ctttctgcgt ttataccaac gtcctgactg gtataatgag 1981 ccagttctta aaatcgcata aggtacataa ggtaattcac aatgattaaa gttgaaatta 2041 aaccatctca agcccaattt actactcgtt ctggtgtttc tcgtcagggc aagccttatt 2101 cactgaatga gcagctttgt tacgttgatt tgggtaatga atatccggtt cttgtcaaga 2161 ttactcttga tgaaggtcag ccagcctatg cgcctggtct gtacaccgtt catctgtcct 2221 ctttcaaagt tggtcagttc ggttccctta tgattgaccg tctgcgcctc gttccggcta 2281 agtaaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 2341 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 2401 tttctgcgtt tatagttccg gctaagtaac tactagatgg agcaggtcgc ggatttcgac 2461 acaatttatc aggcgatgat acaaatctcc gttgtacttt gtttcgcgct tggtataatc 2521 gctgggggtc aaagatgacc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 2581 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 2641 cttcgggtgg gcctttctgc gtttatatcg ctgggggtca aagatgagtg ttttagtgta 2701 ttctttcgcc tctttcgttt taggttggtg ccttcgtagt ggcattacgt attttacccg 2761 tttaatggaa acttcctcat gaccaggcat caaataaaac gaaaggctca gtcgaaagac 2821 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 2881 tcaccttcgg gtgggccttt ctgcgtttat aaatctccgt tgtactttgt ttcgcgcttg 2941 gtataatcgc tgggggtcta atggaaactt cctcatgaaa aagtctttag tcctcaaagc 3001 ctctgtagcc gttgctaccc tcgttccgat gctgtctttc gctgctgagg gtgacgatcc 3061 cgcaaaagcg gcctttaact ccctgcaagc ctcagcgacc gaatatatcg gttatgcgtg 3121 ggcgatggtt gttgtcattg tcggcgcaac tatcggtatc aagctgttta agaaattcac 3181 ctcgaaagca agctgaccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 3241 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 3301 tcgggtgggc ctttctgcgt ttataaattc acctcgaaag caagctgata aaccgataca 3361 attaaaggct ccttttggag attttcaacg tgaaaaaatt attattcgca attcctttag 3421 ttgttccttt ctattctcac tccgctgaaa ctgttgaaag ttgtttagca aaaccccata 3481 cagaaaattc atttactaac gtctggaaag acgacaaaac tttagatcgt tacgctaact 3541 atgagggttg tctgtggaat gctacaggcg ttgtagtttg tactggtgac gaaactcagt 3601 gttacggtac atgggttcct attgggcttg ctatccctga aaatgagggt ggtggctctg 3661 agggtggcgg ttctgagggt ggcggttctg agggtggcgg tactaaacct cctgagtacg 3721 gtgatacacc tattccgggc tatacttata tcaaccctct cgacggcact tatccgcctg 3781 gtactgagca aaaccccgct aatcctaatc cttctcttga ggagtctcag cctcttaata 3841 ctttcatgtt tcagaataat aggttccgaa ataggcaggg ggcattaact gtttatacgg 3901 gcactgttac tcaaggcact gaccccgtta aaacttatta ccagtacact cctgtatcat 3961 caaaagccat gtatgacgct tactggaacg gtaaattcag agactgcgct ttccattctg 4021 gctttaatga ggatccattc gtttgtgaat atcaaggcca atcgtctgac ctgcctcaac 4081 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg 4141 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct 4201 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg 4261 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta 4321 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta 4381 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg 4441 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg 4501 aatgtcgccc ttttgtcttt agcgctggta aaccatatga attttctatt gattgtgaca 4561 aaataaactt attccgtggt gtctttgcgt ttcttttata tgttgccacc tttatgtatg 4621 tattttctac gtttgctaac atactgcgta ataaggagtc ttaa //