LOCUS BBa_M31790 3842 bp DNA linear 27-Dec-2024 DEFINITION Insert to detect osmolarity ACCESSION BBa_M31790 VERSION BBa_M31790.1 FEATURES Location/Qualifiers misc_feature 381..428 /label=ex-Gene X Promoter misc_feature 480..495 /label=ex-Gene X RBS CDS 1..831 /label=BBa_M31742 promoter 832..879 /label=BBa_M13110 RBS 880..895 /label=BBa_M13510 misc_feature 1186..1231 /label=partial ex-Gene V Promoter CDS 896..1231 /label=BBa_M31744 promoter 1232..1281 /label=BBa_M13105 RBS 1282..1297 /label=BBa_M13505 misc_feature 1547..1561 /label=ex-Gene VII RBS CDS 1298..1561 /label=BBa_M31746 RBS 1562..1577 /label=BBa_M13507 misc_feature 1625..1671 /label=ex-Gene VIII promoter misc_feature 1660..1675 /label=ex-Gene IX RBS CDS 1578..1679 /label=BBa_M31748 RBS 1680..1695 /label=BBa_M13509 misc_feature 1775..1790 /label=ex-Gene VIII RBS CDS 1696..1794 /label=BBa_M31780 promoter 1795..1841 /label=BBa_M13108 RBS 1842..1857 /label=BBa_M13508 misc_feature 2057..2079 /label=ex-Gene III Promoter CDS 1858..2079 /label=BBa_M31782 misc_feature 2092..2109 /label=C1 OmpR misc_feature 2113..2130 /label=C2 OmpR misc_feature 2133..2150 /label=C3 OmpR promoter 2154..2159 /label=-35 promoter 2177..2182 /label=-10 promoter 2080..2187 /label=BBa_R0082 RBS 2188..2200 /label=RBS-3\Weak RBS 2194..2197 /label=RBS misc_feature 2188..2200 /label=BBa_B0032 RBS 2188..2200 /label=BBa_B0032 misc_feature 2201..2914 /label=mCherry misc_feature 2915..3136 /label=gene VIII misc_feature 3114..3136 /label=ex-Gene X RBS CDS 2201..3136 /label=BBa_M31784 promoter 3137..3184 /label=BBa_M13103 RBS 3185..3200 /label=BBa_M13503 CDS 3201..3842 /label=BBa_M31786 ORIGIN 1 aacgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat 61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact 121 cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta 181 gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca 241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg 301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag 361 tctttcgggc ttcctcttaa tctcttcgac gcaatccgct tcgcctccga ctataatagc 421 caagggaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca 481 ttcgaaggtg actctatgaa tatttatgac gattccgcag tattggacgc tatccagtct 541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt 601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt 661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg 721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt 781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata atctttttga 841 tgcaatccgc tttgcttctg actataatag tcagggtaaa tttgaggggg attcaatgaa 901 tatttatgac gattccgcag tattggacgc tatccagtct aaacatttta ctattacccc 961 ctctggcaaa acttcttttg caaaagcctc tcgctatttt ggtttttatc gtcgtctggt 1021 aaacgagggt tatgatagtg ttgctcttac tatgcctcgt aattcctttt ggcgttatgt 1081 atctgcatta gttgaatgtg gtattcctaa atctcaactg atgaatcttt ctacctgtaa 1141 taatgttgtt ccgttagttc gttttattaa cgtagatttt tcttcccagc gtccagattg 1201 gtataacgag cccgttctca aaattgcata gccaacgtcc tgactggtat aatgagccag 1261 ttcttaaaat cgcataaggt acataaggta attcacaatg attaaagttg aaattaaacc 1321 atctcaagcc caatttacta ctcgttctgg tgtttctcgt cagggcaagc cttattcact 1381 gaatgagcag ctttgttacg ttgatttggg taatgaatat ccggttcttg tcaagattac 1441 tcttgatgaa ggtcagccag cctatgcgcc tggtctgtac accgttcatc tgtcctcttt 1501 caaagttggt cagttcggtt cccttatgat tgaccgtctg cgcctcgtcc cagccaaata 1561 agttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat caggcgatga 1621 tacaaatatc tgtcgtgctc tgcttcgcac tcggtattat cgccggtggg cagagatgat 1681 cgctgggggt caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg 1741 tgccttcgta gtggcattac gtattttacc cgtttgatgg agacatcttc atgaaatctc 1801 cgttgtactt tgtttcgcgc ttggtataat cgctgggggt ctaatggaaa cttcctcatg 1861 aaaaagtctt tagtcctcaa agcctctgta gccgttgcta ccctcgttcc gatgctgtct 1921 ttcgctgctg agggtgacga tcccgcaaaa gcggccttta actccctgca agcctcagcg 1981 accgaatata tcggttatgc gtgggcgatg gttgttgtca ttgtcggcgc aactatcggt 2041 atcaagctgt ttaagaaatt tacatccaag gctagttgat cccttgcatt tacattttga 2101 aacatctata gcgataaatg aaacatctta aaagttttag tatcatattc gtgttggatt 2161 attctgcatt tttggggaga atggacttca cacaggaaag atggtgagca agggcgagga 2221 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt 2281 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca 2341 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc 2401 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta 2461 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg 2521 cggcgtggtg accgtgaccc aggactcctc cttgcaggac ggcgagttca tctacaaggt 2581 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg 2641 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa 2701 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa 2761 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac 2821 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc 2881 caccggcggc atggacgagc tgtacaagta ataaatgaaa aagtctttag tcctcaaagc 2941 ctctgtagcc gttgctaccc tcgttccgat gctgtctttc gctgctgagg gtgacgatcc 3001 cgcaaaagcg gcctttaact ccctgcaagc ctcagcgacc gaatatatcg gttatgcgtg 3061 ggcgatggtt gttgtcattg tcggcgcaac tatcggtatc aagctgttta agaaatttac 3121 atccaaggct agttgaaatt cacctcgaaa gcaagctgat aaaccgatac aattaaaggc 3181 tccttttgga gattttcaac gtgaaaaaat tattattcgc aattccttta gttgttcctt 3241 tctattctca ctccgctgaa actgttgaaa gttgtttagc aaaaccccat acagaaaatt 3301 catttactaa cgtctggaaa gacgacaaaa ctttagatcg ttacgctaac tatgagggtt 3361 gtctgtggaa tgctacaggc gttgtagttt gtactggtga cgaaactcag tgttacggta 3421 catgggttcc tattgggctt gctatccctg aaaatgaggg tggtggctct gagggtggcg 3481 gttctgaggg tggcggttct gagggtggcg gtactaaacc tcctgagtac ggtgatacac 3541 ctattccggg ctatacttat atcaaccctc tcgacggcac ttatccgcct ggtactgagc 3601 aaaaccccgc taatcctaat ccttctcttg aggagtctca gcctcttaat actttcatgt 3661 ttcagaataa taggttccga aataggcagg gggcattaac tgtttatacg ggcactgtta 3721 ctcaaggcac tgaccccgtt aaaacttatt accagtacac tcctgtatca tcaaaagcca 3781 tgtatgacgc ttactggaac ggtaaattca gagactgcgc tttccattct ggctttaatg 3841 ag //