BBa_M31746 1 BBa_M31746 Gene V w/o RBS for Gene VII 2007-02-28T12:00:00Z 2015-05-08T01:14:00Z M13K07 Gene V This produces Protein V and still has Gene VII promoter (if one exists) intact. It does not have a Gene VII RBS. false false _102_ 0 1374 102 Not in stock false I could not destroy the Gene VII promoter even though this would be ideal for refactoring. This promoter has not been identified, but I suspect that, if it exists, it sits somewhere in this gene. false Emilienne Repak annotation1917256 1 ex-Gene VII RBS range1917256 1 250 264 BBa_M13505 1 BBa_M13505 M13KO7 gene V RBS 2006-12-14T12:00:00Z 2015-05-08T01:13:56Z New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban This RBS is part of the bacteriophage M13 genome, initiating translation of the gene V message (BBa_M13005). The part aligns with base pairs 827-842 in M13K07 genome. It was identified as the RBS for the gene V message by Wezenbeek et al in Gene (1980) 11: 129-148 false false _45_ 0 314 1 Not in stock false none false Natalie Kuldell BBa_M13507 1 BBa_M13507 M13KO7 gene VII RBS 2006-12-14T12:00:00Z 2015-05-08T01:13:56Z New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VII message (BBa_M13007). The part aligns with base pairs 1092-1107 in M13K07 genome. It was identified as the RBS for gene VII message by Wezenbeek et al in Gene (1980) 11: 129-148 false true _45_ 0 314 1 Not in stock false New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban false Natalie Kuldell BBa_M31780 1 BBa_M31780 Gene IX w/o Gene VIII RBS 2007-02-28T12:00:00Z 2015-05-08T01:14:00Z M13K07 Gene IX This produces Protein IX but does not contain the Gene VIII RBS. false false _102_ 0 1374 102 Not in stock false I needed to remove the Gene VIII RBS. false Emilienne Repak annotation1917470 1 ex-Gene VIII RBS range1917470 1 80 95 BBa_M31744 1 BBa_M31744 Gene X w/o Promoter for Gene V 2007-02-28T12:00:00Z 2015-05-08T01:14:00Z M13K07 Gene X This part produces the GEne X protein but does not contain a promoter for Gene V. false false _102_ 0 1374 102 Not in stock false I actually didn't have to remove the RBS for Gene V since it is in a space between Genes X and V. I only removed the Gene V promoter. false Emilienne Repak annotation1917246 1 partial ex-Gene V Promoter range1917246 1 291 336 BBa_M31782 1 BBa_M31782 Gene VIII w/o Gene III Promoter 2007-02-28T12:00:00Z 2015-05-08T01:14:00Z M13K07 Gene VIII This produces the Gene VIII protein but does not have the Gene III Promoter. false false _102_ 0 1374 102 Not in stock false I had to remove the Gene III Promoter, but the Gene III RBS site is between Genes VIII and III, so I didn't have to worry about that. false Emilienne Repak annotation1917480 1 ex-Gene III Promoter range1917480 1 200 222 BBa_M13110 1 BBa_M13110 M13110 2006-12-15T12:00:00Z 2015-05-08T01:13:55Z New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban. This part is from the bacteriophage M13 genome, bp 381-428 in M13K07. It directs transcription of M13 gene X (BBa_M13510, BBa_M13010). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP. false false _45_ 0 314 1 Not in stock false none false Natalie Kuldell BBa_M13509 1 BBa_M13509 M13Ko7 gene IX RBS 2006-12-14T12:00:00Z 2015-05-08T01:13:56Z New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban This RBS is part of the bacteriophage M13 genome, initiating translation of the gene IX message (BBa_M13009). The part aligns with base pairs 1190-1205 in M13K07 genome. It was identified as the RBS for gene IX message by Wezenbeek et al in Gene (1980) 11: 129-148 false false _45_ 0 314 1 Not in stock false none false Natalie Kuldell BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1710 1 RBS range1710 1 7 10 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation7027 1 BBa_B0032 range7027 1 1 13 BBa_M13510 1 BBa_M13510 M13KO7 gene X RBS 2006-12-14T12:00:00Z 2015-05-08T01:13:56Z New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban This RBS is part of the bacteriophage M13 genome, initiating translation of the gene X message (BBa_M13010). The part aligns with base pairs 480-495 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148 false false _45_ 0 314 1 Not in stock false none false Natalie Kuldell BBa_M13503 1 BBa_M13503 M13K07 gene III RBS 2006-12-14T12:00:00Z 2015-05-08T01:13:56Z New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban This RBS is part of the bacteriophage M13 genome, initiating translation of the gene III message (BBa_M13003). The part aligns with base pairs 1563-1578 in M13K07 genome. It was identified as the RBS for gene III message by Wezenbeek et al in Gene (1980) 11: 129-148 New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban false false _45_ 0 314 1 Not in stock false none false Natalie Kuldell BBa_M13508 1 BBa_M13508 M13K07 gene VIII RBS 2006-12-14T12:00:00Z 2015-05-08T01:13:56Z New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VIII message (BBa_M13008). The part aligns with base pairs 1285-1300 in M13K07 genome. It was identified as the RBS for gene VIII message by Wezenbeek et al in Gene (1980) 11: 129-148 false true _45_ 0 314 1 Not in stock false none false Natalie Kuldell BBa_M31786 1 BBa_M31786 1st half of Gene III- preBamHI cut 2007-02-28T12:00:00Z 2015-05-08T01:14:00Z M13K07 Gene III This produces the first half of Protein III, up to where Gene III is cut by BamHI. false false _102_ 0 1374 102 Not in stock false I had to find out where BamHI cuts this gene. false Emilienne Repak BBa_M31790 1 BBa_M31790 Insert to detect osmolarity 2007-02-28T12:00:00Z 2015-05-08T01:14:00Z Parts from the Standard Registry of parts, M13K07, and my refactored genes. This insert, when placed in between HpaI and BamHI restriction sites in M13K07, in the right orientation, should be capable of infecting E.coli and, when the environment has high molarity, the phage produced will express mCherry on their coat. false false _102_ 0 1374 102 Not in stock false I had to consider the order in which to place everything and carefully refactor some of the basic parts I will use. false Emilienne Repak component1918741 1 BBa_M31742 component1918753 1 BBa_M31748 component1918745 1 BBa_M31744 component1918754 1 BBa_M13509 component1918774 1 BBa_M13103 component1918746 1 BBa_M13105 component1918757 1 BBa_M13108 component1918758 1 BBa_M13508 component1918747 1 BBa_M13505 component1918749 1 BBa_M31746 component1918760 1 BBa_M31782 component1918756 1 BBa_M31780 component1918775 1 BBa_M13503 component1918743 1 BBa_M13510 component1918742 1 BBa_M13110 component1918773 1 BBa_M31784 component1918768 1 BBa_B0032 component1918776 1 BBa_M31786 component1918766 1 BBa_R0082 component1918750 1 BBa_M13507 annotation1918745 1 BBa_M31744 range1918745 1 896 1231 annotation1918773 1 BBa_M31784 range1918773 1 2201 3136 annotation1918760 1 BBa_M31782 range1918760 1 1858 2079 annotation1918758 1 BBa_M13508 range1918758 1 1842 1857 annotation1918776 1 BBa_M31786 range1918776 1 3201 3842 annotation1918766 1 BBa_R0082 range1918766 1 2080 2187 annotation1918775 1 BBa_M13503 range1918775 1 3185 3200 annotation1918742 1 BBa_M13110 range1918742 1 832 879 annotation1918743 1 BBa_M13510 range1918743 1 880 895 annotation1918741 1 BBa_M31742 range1918741 1 1 831 annotation1918756 1 BBa_M31780 range1918756 1 1696 1794 annotation1918768 1 BBa_B0032 range1918768 1 2188 2200 annotation1918753 1 BBa_M31748 range1918753 1 1578 1679 annotation1918754 1 BBa_M13509 range1918754 1 1680 1695 annotation1918747 1 BBa_M13505 range1918747 1 1282 1297 annotation1918750 1 BBa_M13507 range1918750 1 1562 1577 annotation1918774 1 BBa_M13103 range1918774 1 3137 3184 annotation1918749 1 BBa_M31746 range1918749 1 1298 1561 annotation1918757 1 BBa_M13108 range1918757 1 1795 1841 annotation1918746 1 BBa_M13105 range1918746 1 1232 1281 BBa_M31784 1 BBa_M31784 Gene VIII w/o Gene III Promoter + w/ mCherry 2007-02-28T12:00:00Z 2015-05-08T01:14:00Z M13K07 and the Standard Registry of Parts, thanks to work done in Roger Tsien's laboratory at UCSD. This produces gene VIII (without the Gene III promoter) with mCherry fused to it. false false _102_ 0 1374 102 Not in stock false I had to remove the Gene III Promoter. Then I had to try to add the mCherry onto the extracellular side of protein VIII, which I believe is the N-terminus, which I think is coded on the 5' side of the gene, so I added mCherry before Gene VIII. false Emilienne Repak annotation1917541 1 gene VIII range1917541 1 715 936 annotation1917539 1 mCherry range1917539 1 1 714 annotation1917575 1 ex-Gene X RBS range1917575 1 914 936 BBa_M13103 1 BBa_M13103 M13K07 gene III promoter 2006-12-15T12:00:00Z 2015-05-08T01:13:55Z New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban. This part is from the bacteriophage M13 genome, bp 1500-1547 in M13K07. It directs transcription of M13 gene III (BBa_M13503, BBa_M13003). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP. false true _45_ 0 314 1 Not in stock false none false Natalie Kuldell BBa_M13105 1 BBa_M13105 M13K07 gene V promoter 2006-12-15T12:00:00Z 2015-05-08T01:13:55Z New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban. This part is from the bacteriophage M13 genome, bp 786-835 in M13K07. It directs transcription of M13 gene V (BBa_M13505, BBa_M13005). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP. false false _45_ 0 314 1 Not in stock false none false Natalie Kuldell BBa_M31748 1 BBa_M31748 Gene VII w/o Genes IX RBS or VIII Promoter 2007-02-28T12:00:00Z 2015-05-08T01:14:00Z M13K07 Gene VII This produces Protein VII but does not contain the promoter region for Genes VIII or RBS for Gene IX. It may contain a Gene IX promoter. false false _102_ 0 1374 102 Not in stock false I realized that the Gene VIII promoter is actually within this gene, which surprised me, and I had to remove that along with the RBS for Gene IX. I suspect that the Gene IX promoter, should one exist, may be in this gene. false Emilienne Repak annotation1917397 1 ex-Gene IX RBS range1917397 1 83 98 annotation1917351 1 ex-Gene VIII promoter range1917351 1 48 94 BBa_M31742 1 BBa_M31742 Gene II after HpaI cut 2007-02-27T12:00:00Z 2015-05-08T01:14:00Z From M13 It is the second half of gene II after HpaI cuts gene II. I removed the Gene X Promoter and RBS sequences. false false _102_ 0 1374 102 Not in stock false I altered the Gene X Promoter and RBS sequences false Emilienne Repak annotation1917210 1 ex-Gene X Promoter range1917210 1 381 428 annotation1917211 1 ex-Gene X RBS range1917211 1 480 495 BBa_M13108 1 BBa_M13108 M13K07 gene VIII promoter 2006-12-15T12:00:00Z 2015-05-08T01:13:55Z New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban. This part is from the bacteriophage M13 genome, bp 1155-1201 in M13K07. It directs transcription of M13 gene VIII (BBa_M13508, BBa_M13008). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP. false true _45_ 0 314 1 Not in stock false none false Natalie Kuldell BBa_R0082 1 OmpR Promoter (OmpR, positive) 2004-01-27T12:00:00Z 2015-05-08T01:14:15Z NC_000193 E. coli K12 Released HQ 2013 Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. false false _1_ 0 24 7 In stock false The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown. true Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004) annotation301167 1 -10 range301167 1 98 103 annotation301166 1 -35 range301166 1 75 80 annotation301154 1 C1 OmpR range301154 1 13 30 annotation301155 1 C2 OmpR range301155 1 34 51 annotation301156 1 C3 OmpR range301156 1 54 71 BBa_M31790_sequence 1 aacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctcttcgacgcaatccgcttcgcctccgactataatagccaagggaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcattcgaaggtgactctatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccagcgtccagattggtataacgagcccgttctcaaaattgcatagccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtacataaggtaattcacaatgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgtcccagccaaataagttccggctaagtaacatggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatatctgtcgtgctctgcttcgcactcggtattatcgccggtgggcagagatgatcgctgggggtcaaagatgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttgatggagacatcttcatgaaatctccgttgtactttgtttcgcgcttggtataatcgctgggggtctaatggaaacttcctcatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaatttacatccaaggctagttgatcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttcacacaggaaagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataaatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaatttacatccaaggctagttgaaattcacctcgaaagcaagctgataaaccgatacaattaaaggctccttttggagattttcaacgtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccgcctggtactgagcaaaaccccgctaatcctaatccttctcttgaggagtctcagcctcttaatactttcatgtttcagaataataggttccgaaataggcagggggcattaactgtttatacgggcactgttactcaaggcactgaccccgttaaaacttattaccagtacactcctgtatcatcaaaagccatgtatgacgcttactggaacggtaaattcagagactgcgctttccattctggctttaatgag BBa_M31744_sequence 1 atgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccagcgtccagattggtataacgagcccgttctcaaaattgcatag BBa_R0082_sequence 1 tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact BBa_M13503_sequence 1 tttggagattttcaac BBa_M13105_sequence 1 ccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggta BBa_M31782_sequence 1 atgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaatttacatccaaggctagttga BBa_M13108_sequence 1 aatctccgttgtactttgtttcgcgcttggtataatcgctgggggtc BBa_M13507_sequence 1 gttccggctaagtaac BBa_M31786_sequence 1 gtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccgcctggtactgagcaaaaccccgctaatcctaatccttctcttgaggagtctcagcctcttaatactttcatgtttcagaataataggttccgaaataggcagggggcattaactgtttatacgggcactgttactcaaggcactgaccccgttaaaacttattaccagtacactcctgtatcatcaaaagccatgtatgacgcttactggaacggtaaattcagagactgcgctttccattctggctttaatgag BBa_M31748_sequence 1 atggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatatctgtcgtgctctgcttcgcactcggtattatcgccggtgggcagagatga BBa_M13510_sequence 1 atttgagggggattca BBa_M13103_sequence 1 aattcacctcgaaagcaagctgataaaccgatacaattaaaggctcct BBa_M31780_sequence 1 atgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttgatggagacatcttcatga BBa_M31746_sequence 1 atgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgtcccagccaaataa BBa_M31784_sequence 1 atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataaatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaatttacatccaaggctagttga BBa_M13508_sequence 1 taatggaaacttcctc BBa_B0032_sequence 1 tcacacaggaaag BBa_M31742_sequence 1 aacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctcttcgacgcaatccgcttcgcctccgactataatagccaagggaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcattcgaaggtgactctatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataa BBa_M13505_sequence 1 cataaggtaattcaca BBa_M13509_sequence 1 tcgctgggggtcaaag BBa_M13110_sequence 1 tctttttgatgcaatccgctttgcttctgactataatagtcagggtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z