BBa_M31790
type: ComponentDefinition
persistent identity: BBa M31790
display id: BBa_M31790
version: 1
was generated by: iGEM to SBOL conversion
description: Insert to detect osmolarity
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: Parts from the Standard Registry of parts, M13K07, and my refactored genes.
owned by: james
owned by: myers
mutable description: This insert, when placed in between HpaI and BamHI restriction sites in M13K07, in the right orientation, should be capable of infecting E.coli and, when the environment has high molarity, the phage produced will express mCherry on their coat.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1374
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: I had to consider the order in which to place everything and carefully refactor some of the basic parts I will use.
star: false
creator: Emilienne Repak
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_M13510
component: BBa_M13110
component: BBa_M31742
component: BBa_M31746
component: BBa_M13505
component: BBa_M13105
component: BBa_M31744
component: BBa_M31780
component: BBa_M13509
component: BBa_M31748
component: BBa_M13507
component: BBa_R0082
component: BBa_M31782
component: BBa_M13508
component: BBa_M13108
component: BBa_M13503
component: BBa_M13103
component: BBa_M31784
component: BBa_B0032
component: BBa_M31786
sequence annotation: BBa_M31742
sequence annotation: BBa_M13110
sequence annotation: BBa_M13510
sequence annotation: BBa_M31744
sequence annotation: BBa_M13105
sequence annotation: BBa_M13505
sequence annotation: BBa_M31746
sequence annotation: BBa_M13507
sequence annotation: BBa_M31748
sequence annotation: BBa_M13509
sequence annotation: BBa_M31780
sequence annotation: BBa_M13108
sequence annotation: BBa_M13508
sequence annotation: BBa_M31782
sequence annotation: BBa_R0082
sequence annotation: BBa_B0032
sequence annotation: BBa_M31784
sequence annotation: BBa_M13103
sequence annotation: BBa_M13503
sequence annotation: BBa_M31786
sequence: https://synbiohub.org/public/igem/BBa_M31790_sequence/1

BBa_M13510
type: Component
persistent identity: component1918743
display id: component1918743
version: 1
top level: BBa_M31790
definition: BBa_M13510
access: public

BBa_M13110
type: Component
persistent identity: component1918742
display id: component1918742
version: 1
top level: BBa_M31790
definition: BBa_M13110
access: public

BBa_M31742
type: Component
persistent identity: component1918741
display id: component1918741
version: 1
top level: BBa_M31790
definition: BBa_M31742
access: public

BBa_M31746
type: Component
persistent identity: component1918749
display id: component1918749
version: 1
top level: BBa_M31790
definition: BBa_M31746
access: public

BBa_M13505
type: Component
persistent identity: component1918747
display id: component1918747
version: 1
top level: BBa_M31790
definition: BBa_M13505
access: public

BBa_M13105
type: Component
persistent identity: component1918746
display id: component1918746
version: 1
top level: BBa_M31790
definition: BBa_M13105
access: public

BBa_M31744
type: Component
persistent identity: component1918745
display id: component1918745
version: 1
top level: BBa_M31790
definition: BBa_M31744
access: public

BBa_M31780
type: Component
persistent identity: component1918756
display id: component1918756
version: 1
top level: BBa_M31790
definition: BBa_M31780
access: public

BBa_M13509
type: Component
persistent identity: component1918754
display id: component1918754
version: 1
top level: BBa_M31790
definition: BBa_M13509
access: public

BBa_M31748
type: Component
persistent identity: component1918753
display id: component1918753
version: 1
top level: BBa_M31790
definition: BBa_M31748
access: public

BBa_M13507
type: Component
persistent identity: component1918750
display id: component1918750
version: 1
top level: BBa_M31790
definition: BBa_M13507
access: public

BBa_R0082
type: Component
persistent identity: component1918766
display id: component1918766
version: 1
top level: BBa_M31790
definition: OmpR
access: public

BBa_M31782
type: Component
persistent identity: component1918760
display id: component1918760
version: 1
top level: BBa_M31790
definition: BBa_M31782
access: public

BBa_M13508
type: Component
persistent identity: component1918758
display id: component1918758
version: 1
top level: BBa_M31790
definition: BBa_M13508
access: public

BBa_M13108
type: Component
persistent identity: component1918757
display id: component1918757
version: 1
top level: BBa_M31790
definition: BBa_M13108
access: public

BBa_M13503
type: Component
persistent identity: component1918775
display id: component1918775
version: 1
top level: BBa_M31790
definition: BBa_M13503
access: public

BBa_M13103
type: Component
persistent identity: component1918774
display id: component1918774
version: 1
top level: BBa_M31790
definition: BBa_M13103
access: public

BBa_M31784
type: Component
persistent identity: component1918773
display id: component1918773
version: 1
top level: BBa_M31790
definition: BBa_M31784
access: public

BBa_B0032
type: Component
persistent identity: component1918768
display id: component1918768
version: 1
top level: BBa_M31790
definition: BBa_B0032
access: public

BBa_M31786
type: Component
persistent identity: component1918776
display id: component1918776
version: 1
top level: BBa_M31790
definition: BBa_M31786
access: public

BBa_M31742
type: SequenceAnnotation
persistent identity: annotation1918741
display id: annotation1918741
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918741/range1918741/1
component: BBa_M31742

https://synbiohub.org/public/igem/BBa_M31790/annotation1918741/range1918741/1
type: Range
persistent identity: range1918741
display id: range1918741
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 1
end: 831
orientation: inline

BBa_M13110
type: SequenceAnnotation
persistent identity: annotation1918742
display id: annotation1918742
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918742/range1918742/1
component: BBa_M13110

https://synbiohub.org/public/igem/BBa_M31790/annotation1918742/range1918742/1
type: Range
persistent identity: range1918742
display id: range1918742
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 832
end: 879
orientation: inline

BBa_M13510
type: SequenceAnnotation
persistent identity: annotation1918743
display id: annotation1918743
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918743/range1918743/1
component: BBa_M13510

https://synbiohub.org/public/igem/BBa_M31790/annotation1918743/range1918743/1
type: Range
persistent identity: range1918743
display id: range1918743
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 880
end: 895
orientation: inline

BBa_M31744
type: SequenceAnnotation
persistent identity: annotation1918745
display id: annotation1918745
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918745/range1918745/1
component: BBa_M31744

https://synbiohub.org/public/igem/BBa_M31790/annotation1918745/range1918745/1
type: Range
persistent identity: range1918745
display id: range1918745
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 896
end: 1231
orientation: inline

BBa_M13105
type: SequenceAnnotation
persistent identity: annotation1918746
display id: annotation1918746
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918746/range1918746/1
component: BBa_M13105

https://synbiohub.org/public/igem/BBa_M31790/annotation1918746/range1918746/1
type: Range
persistent identity: range1918746
display id: range1918746
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 1232
end: 1281
orientation: inline

BBa_M13505
type: SequenceAnnotation
persistent identity: annotation1918747
display id: annotation1918747
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918747/range1918747/1
component: BBa_M13505

https://synbiohub.org/public/igem/BBa_M31790/annotation1918747/range1918747/1
type: Range
persistent identity: range1918747
display id: range1918747
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 1282
end: 1297
orientation: inline

BBa_M31746
type: SequenceAnnotation
persistent identity: annotation1918749
display id: annotation1918749
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918749/range1918749/1
component: BBa_M31746

https://synbiohub.org/public/igem/BBa_M31790/annotation1918749/range1918749/1
type: Range
persistent identity: range1918749
display id: range1918749
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 1298
end: 1561
orientation: inline

BBa_M13507
type: SequenceAnnotation
persistent identity: annotation1918750
display id: annotation1918750
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918750/range1918750/1
component: BBa_M13507

https://synbiohub.org/public/igem/BBa_M31790/annotation1918750/range1918750/1
type: Range
persistent identity: range1918750
display id: range1918750
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 1562
end: 1577
orientation: inline

BBa_M31748
type: SequenceAnnotation
persistent identity: annotation1918753
display id: annotation1918753
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918753/range1918753/1
component: BBa_M31748

https://synbiohub.org/public/igem/BBa_M31790/annotation1918753/range1918753/1
type: Range
persistent identity: range1918753
display id: range1918753
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 1578
end: 1679
orientation: inline

BBa_M13509
type: SequenceAnnotation
persistent identity: annotation1918754
display id: annotation1918754
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918754/range1918754/1
component: BBa_M13509

https://synbiohub.org/public/igem/BBa_M31790/annotation1918754/range1918754/1
type: Range
persistent identity: range1918754
display id: range1918754
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 1680
end: 1695
orientation: inline

BBa_M31780
type: SequenceAnnotation
persistent identity: annotation1918756
display id: annotation1918756
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918756/range1918756/1
component: BBa_M31780

https://synbiohub.org/public/igem/BBa_M31790/annotation1918756/range1918756/1
type: Range
persistent identity: range1918756
display id: range1918756
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 1696
end: 1794
orientation: inline

BBa_M13108
type: SequenceAnnotation
persistent identity: annotation1918757
display id: annotation1918757
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918757/range1918757/1
component: BBa_M13108

https://synbiohub.org/public/igem/BBa_M31790/annotation1918757/range1918757/1
type: Range
persistent identity: range1918757
display id: range1918757
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 1795
end: 1841
orientation: inline

BBa_M13508
type: SequenceAnnotation
persistent identity: annotation1918758
display id: annotation1918758
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918758/range1918758/1
component: BBa_M13508

https://synbiohub.org/public/igem/BBa_M31790/annotation1918758/range1918758/1
type: Range
persistent identity: range1918758
display id: range1918758
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 1842
end: 1857
orientation: inline

BBa_M31782
type: SequenceAnnotation
persistent identity: annotation1918760
display id: annotation1918760
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918760/range1918760/1
component: BBa_M31782

https://synbiohub.org/public/igem/BBa_M31790/annotation1918760/range1918760/1
type: Range
persistent identity: range1918760
display id: range1918760
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 1858
end: 2079
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation1918766
display id: annotation1918766
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918766/range1918766/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_M31790/annotation1918766/range1918766/1
type: Range
persistent identity: range1918766
display id: range1918766
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 2080
end: 2187
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1918768
display id: annotation1918768
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918768/range1918768/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_M31790/annotation1918768/range1918768/1
type: Range
persistent identity: range1918768
display id: range1918768
version: 1
top level: BBa_M31790
direction: direction/forward
start: 2188
end: 2200
orientation: inline

BBa_M31784
type: SequenceAnnotation
persistent identity: annotation1918773
display id: annotation1918773
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918773/range1918773/1
component: BBa_M31784

https://synbiohub.org/public/igem/BBa_M31790/annotation1918773/range1918773/1
type: Range
persistent identity: range1918773
display id: range1918773
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 2201
end: 3136
orientation: inline

BBa_M13103
type: SequenceAnnotation
persistent identity: annotation1918774
display id: annotation1918774
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918774/range1918774/1
component: BBa_M13103

https://synbiohub.org/public/igem/BBa_M31790/annotation1918774/range1918774/1
type: Range
persistent identity: range1918774
display id: range1918774
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 3137
end: 3184
orientation: inline

BBa_M13503
type: SequenceAnnotation
persistent identity: annotation1918775
display id: annotation1918775
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918775/range1918775/1
component: BBa_M13503

https://synbiohub.org/public/igem/BBa_M31790/annotation1918775/range1918775/1
type: Range
persistent identity: range1918775
display id: range1918775
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 3185
end: 3200
orientation: inline

BBa_M31786
type: SequenceAnnotation
persistent identity: annotation1918776
display id: annotation1918776
version: 1
top level: BBa_M31790
location: https://synbiohub.org/public/igem/BBa_M31790/annotation1918776/range1918776/1
component: BBa_M31786

https://synbiohub.org/public/igem/BBa_M31790/annotation1918776/range1918776/1
type: Range
persistent identity: range1918776
display id: range1918776
version: 1
top level: BBa_M31790
direction: direction/reverse
start: 3201
end: 3842
orientation: inline

BBa_M13510
type: ComponentDefinition
persistent identity: BBa M13510
display id: BBa_M13510
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene X RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene X message (BBa_M13010). The part aligns with base pairs 480-495 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13510_sequence/1

BBa_M13505
type: ComponentDefinition
persistent identity: BBa M13505
display id: BBa_M13505
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene V RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene V message (BBa_M13005). The part aligns with base pairs 827-842 in M13K07 genome. It was identified as the RBS for the gene V message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13505_sequence/1

BBa_M13507
type: ComponentDefinition
persistent identity: BBa M13507
display id: BBa_M13507
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene VII RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VII message (BBa_M13007). The part aligns with base pairs 1092-1107 in M13K07 genome. It was identified as the RBS for gene VII message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13507_sequence/1

BBa_M13509
type: ComponentDefinition
persistent identity: BBa M13509
display id: BBa_M13509
version: 1
was generated by: iGEM to SBOL conversion
description: M13Ko7 gene IX RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene IX message (BBa_M13009). The part aligns with base pairs 1190-1205 in M13K07 genome. It was identified as the RBS for gene IX message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13509_sequence/1

BBa_M13508
type: ComponentDefinition
persistent identity: BBa M13508
display id: BBa_M13508
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene VIII RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VIII message (BBa_M13008). The part aligns with base pairs 1285-1300 in M13K07 genome. It was identified as the RBS for gene VIII message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13508_sequence/1

BBa_M13110
type: ComponentDefinition
persistent identity: BBa M13110
display id: BBa_M13110
version: 1
was generated by: iGEM to SBOL conversion
description: M13110
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 381-428 in M13K07. It directs transcription of M13 gene X (BBa_M13510, BBa_M13010). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13110_sequence/1

BBa_M13105
type: ComponentDefinition
persistent identity: BBa M13105
display id: BBa_M13105
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene V promoter
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 786-835 in M13K07. It directs transcription of M13 gene V (BBa_M13505, BBa_M13005). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13105_sequence/1

BBa_M13108
type: ComponentDefinition
persistent identity: BBa M13108
display id: BBa_M13108
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene VIII promoter
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 1155-1201 in M13K07. It directs transcription of M13 gene VIII (BBa_M13508, BBa_M13008). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13108_sequence/1

BBa_M13103
type: ComponentDefinition
persistent identity: BBa M13103
display id: BBa_M13103
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene III promoter
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 1500-1547 in M13K07. It directs transcription of M13 gene III (BBa_M13503, BBa_M13003). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_M13103_sequence/1

BBa_M31742
type: ComponentDefinition
persistent identity: BBa M31742
display id: BBa_M31742
version: 1
was generated by: iGEM to SBOL conversion
description: Gene II after HpaI cut
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: From M13
owned by: james
owned by: myers
mutable description: It is the second half of gene II after HpaI cuts gene II. I removed the Gene X Promoter and RBS sequences.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1374
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: I altered the Gene X Promoter and RBS sequences
star: false
creator: Emilienne Repak
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ex-Gene X Promoter
sequence annotation: ex-Gene X RBS
sequence: https://synbiohub.org/public/igem/BBa_M31742_sequence/1

ex-Gene X Promoter
type: SequenceAnnotation
persistent identity: annotation1917210
display id: annotation1917210
version: 1
top level: BBa_M31742
location: https://synbiohub.org/public/igem/BBa_M31742/annotation1917210/range1917210/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31742/annotation1917210/range1917210/1
type: Range
persistent identity: range1917210
display id: range1917210
version: 1
top level: BBa_M31742
direction: direction/forward
start: 381
end: 428
orientation: inline

ex-Gene X RBS
type: SequenceAnnotation
persistent identity: annotation1917211
display id: annotation1917211
version: 1
top level: BBa_M31742
location: https://synbiohub.org/public/igem/BBa_M31742/annotation1917211/range1917211/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31742/annotation1917211/range1917211/1
type: Range
persistent identity: range1917211
display id: range1917211
version: 1
top level: BBa_M31742
direction: direction/forward
start: 480
end: 495
orientation: inline

BBa_M31744
type: ComponentDefinition
persistent identity: BBa M31744
display id: BBa_M31744
version: 1
was generated by: iGEM to SBOL conversion
description: Gene X w/o Promoter for Gene V
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: M13K07 Gene X
owned by: james
owned by: myers
mutable description: This part produces the GEne X protein but does not contain a promoter for Gene V.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1374
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: I actually didn't have to remove the RBS for Gene V since it is in a space between Genes X and V. I only removed the Gene V promoter.
star: false
creator: Emilienne Repak
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: partial ex-Gene V Promoter
sequence: https://synbiohub.org/public/igem/BBa_M31744_sequence/1

partial ex-Gene V Promoter
type: SequenceAnnotation
persistent identity: annotation1917246
display id: annotation1917246
version: 1
top level: BBa_M31744
location: https://synbiohub.org/public/igem/BBa_M31744/annotation1917246/range1917246/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31744/annotation1917246/range1917246/1
type: Range
persistent identity: range1917246
display id: range1917246
version: 1
top level: BBa_M31744
direction: direction/forward
start: 291
end: 336
orientation: inline

BBa_M31746
type: ComponentDefinition
persistent identity: BBa M31746
display id: BBa_M31746
version: 1
was generated by: iGEM to SBOL conversion
description: Gene V w/o RBS for Gene VII
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: M13K07 Gene V
owned by: james
owned by: myers
mutable description: This produces Protein V and still has Gene VII promoter (if one exists) intact. It does not have a Gene VII RBS.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1374
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: I could not destroy the Gene VII promoter even though this would be ideal for refactoring. This promoter has not been identified, but I suspect that, if it exists, it sits somewhere in this gene.
star: false
creator: Emilienne Repak
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ex-Gene VII RBS
sequence: https://synbiohub.org/public/igem/BBa_M31746_sequence/1

ex-Gene VII RBS
type: SequenceAnnotation
persistent identity: annotation1917256
display id: annotation1917256
version: 1
top level: BBa_M31746
location: https://synbiohub.org/public/igem/BBa_M31746/annotation1917256/range1917256/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31746/annotation1917256/range1917256/1
type: Range
persistent identity: range1917256
display id: range1917256
version: 1
top level: BBa_M31746
direction: direction/forward
start: 250
end: 264
orientation: inline

BBa_M31748
type: ComponentDefinition
persistent identity: BBa M31748
display id: BBa_M31748
version: 1
was generated by: iGEM to SBOL conversion
description: Gene VII w/o Genes IX RBS or VIII Promoter
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: M13K07 Gene VII
owned by: james
owned by: myers
mutable description: This produces Protein VII but does not contain the promoter region for Genes VIII or RBS for Gene IX. It may contain a Gene IX promoter.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1374
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: I realized that the Gene VIII promoter is actually within this gene, which surprised me, and I had to remove that along with the RBS for Gene IX. I suspect that the Gene IX promoter, should one exist, may be in this gene.
star: false
creator: Emilienne Repak
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ex-Gene VIII promoter
sequence annotation: ex-Gene IX RBS
sequence: https://synbiohub.org/public/igem/BBa_M31748_sequence/1

ex-Gene VIII promoter
type: SequenceAnnotation
persistent identity: annotation1917351
display id: annotation1917351
version: 1
top level: BBa_M31748
location: https://synbiohub.org/public/igem/BBa_M31748/annotation1917351/range1917351/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31748/annotation1917351/range1917351/1
type: Range
persistent identity: range1917351
display id: range1917351
version: 1
top level: BBa_M31748
direction: direction/forward
start: 48
end: 94
orientation: inline

ex-Gene IX RBS
type: SequenceAnnotation
persistent identity: annotation1917397
display id: annotation1917397
version: 1
top level: BBa_M31748
location: https://synbiohub.org/public/igem/BBa_M31748/annotation1917397/range1917397/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31748/annotation1917397/range1917397/1
type: Range
persistent identity: range1917397
display id: range1917397
version: 1
top level: BBa_M31748
direction: direction/forward
start: 83
end: 98
orientation: inline

BBa_M31780
type: ComponentDefinition
persistent identity: BBa M31780
display id: BBa_M31780
version: 1
was generated by: iGEM to SBOL conversion
description: Gene IX w/o Gene VIII RBS
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: M13K07 Gene IX
owned by: james
owned by: myers
mutable description: This produces Protein IX but does not contain the Gene VIII RBS.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1374
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: I needed to remove the Gene VIII RBS.
star: false
creator: Emilienne Repak
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ex-Gene VIII RBS
sequence: https://synbiohub.org/public/igem/BBa_M31780_sequence/1

ex-Gene VIII RBS
type: SequenceAnnotation
persistent identity: annotation1917470
display id: annotation1917470
version: 1
top level: BBa_M31780
location: https://synbiohub.org/public/igem/BBa_M31780/annotation1917470/range1917470/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31780/annotation1917470/range1917470/1
type: Range
persistent identity: range1917470
display id: range1917470
version: 1
top level: BBa_M31780
direction: direction/forward
start: 80
end: 95
orientation: inline

BBa_M31782
type: ComponentDefinition
persistent identity: BBa M31782
display id: BBa_M31782
version: 1
was generated by: iGEM to SBOL conversion
description: Gene VIII w/o Gene III Promoter
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: M13K07 Gene VIII
owned by: james
owned by: myers
mutable description: This produces the Gene VIII protein but does not have the Gene III Promoter.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1374
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: I had to remove the Gene III Promoter, but the Gene III RBS site is between Genes VIII and III, so I didn't have to worry about that.
star: false
creator: Emilienne Repak
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ex-Gene III Promoter
sequence: https://synbiohub.org/public/igem/BBa_M31782_sequence/1

ex-Gene III Promoter
type: SequenceAnnotation
persistent identity: annotation1917480
display id: annotation1917480
version: 1
top level: BBa_M31782
location: https://synbiohub.org/public/igem/BBa_M31782/annotation1917480/range1917480/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31782/annotation1917480/range1917480/1
type: Range
persistent identity: range1917480
display id: range1917480
version: 1
top level: BBa_M31782
direction: direction/forward
start: 200
end: 222
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
top level: OmpR
direction: direction/forward
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
top level: OmpR
direction: direction/forward
start: 54
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
top level: OmpR
direction: direction/forward
start: 75
end: 80
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
top level: OmpR
direction: direction/forward
start: 98
end: 103
orientation: inline

BBa_M13503
type: ComponentDefinition
persistent identity: BBa M13503
display id: BBa_M13503
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene III RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene III message (BBa_M13003). The part aligns with base pairs 1563-1578 in M13K07 genome. It was identified as the RBS for gene III message by Wezenbeek et al in Gene (1980) 11: 129-148 New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13503_sequence/1

BBa_M31784
type: ComponentDefinition
persistent identity: BBa M31784
display id: BBa_M31784
version: 1
was generated by: iGEM to SBOL conversion
description: Gene VIII w/o Gene III Promoter + w/ mCherry
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: M13K07 and the Standard Registry of Parts, thanks to work done in Roger Tsien's laboratory at UCSD.
owned by: james
owned by: myers
mutable description: This produces gene VIII (without the Gene III promoter) with mCherry fused to it.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1374
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: I had to remove the Gene III Promoter. Then I had to try to add the mCherry onto the extracellular side of protein VIII, which I believe is the N-terminus, which I think is coded on the 5' side of the gene, so I added mCherry before Gene VIII.
star: false
creator: Emilienne Repak
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mCherry
sequence annotation: gene VIII
sequence annotation: ex-Gene X RBS
sequence: https://synbiohub.org/public/igem/BBa_M31784_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1917539
display id: annotation1917539
version: 1
top level: BBa_M31784
location: https://synbiohub.org/public/igem/BBa_M31784/annotation1917539/range1917539/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31784/annotation1917539/range1917539/1
type: Range
persistent identity: range1917539
display id: range1917539
version: 1
top level: BBa_M31784
direction: direction/forward
start: 1
end: 714
orientation: inline

gene VIII
type: SequenceAnnotation
persistent identity: annotation1917541
display id: annotation1917541
version: 1
top level: BBa_M31784
location: https://synbiohub.org/public/igem/BBa_M31784/annotation1917541/range1917541/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31784/annotation1917541/range1917541/1
type: Range
persistent identity: range1917541
display id: range1917541
version: 1
top level: BBa_M31784
direction: direction/forward
start: 715
end: 936
orientation: inline

ex-Gene X RBS
type: SequenceAnnotation
persistent identity: annotation1917575
display id: annotation1917575
version: 1
top level: BBa_M31784
location: https://synbiohub.org/public/igem/BBa_M31784/annotation1917575/range1917575/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31784/annotation1917575/range1917575/1
type: Range
persistent identity: range1917575
display id: range1917575
version: 1
top level: BBa_M31784
direction: direction/forward
start: 914
end: 936
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_M31786
type: ComponentDefinition
persistent identity: BBa M31786
display id: BBa_M31786
version: 1
was generated by: iGEM to SBOL conversion
description: 1st half of Gene III- preBamHI cut
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: M13K07 Gene III
owned by: james
owned by: myers
mutable description: This produces the first half of Protein III, up to where Gene III is cut by BamHI.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1374
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: I had to find out where BamHI cuts this gene.
star: false
creator: Emilienne Repak
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M31786_sequence/1

https://synbiohub.org/public/igem/BBa_M31790_sequence/1
type: Sequence
persistent identity: BBa M31790 sequence
display id: BBa_M31790_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctcttcgacgcaatccgcttcgcctccgactataatagccaagggaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcattcgaaggtgactctatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccagcgtccagattggtataacgagcccgttctcaaaattgcatagccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtacataaggtaattcacaatgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgtcccagccaaataagttccggctaagtaacatggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatatctgtcgtgctctgcttcgcactcggtattatcgccggtgggcagagatgatcgctgggggtcaaagatgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttgatggagacatcttcatgaaatctccgttgtactttgtttcgcgcttggtataatcgctgggggtctaatggaaacttcctcatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13510_sequence/1
type: Sequence
persistent identity: BBa M13510 sequence
display id: BBa_M13510_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atttgagggggattca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13505_sequence/1
type: Sequence
persistent identity: BBa M13505 sequence
display id: BBa_M13505_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cataaggtaattcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13507_sequence/1
type: Sequence
persistent identity: BBa M13507 sequence
display id: BBa_M13507_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttccggctaagtaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13509_sequence/1
type: Sequence
persistent identity: BBa M13509 sequence
display id: BBa_M13509_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcgctgggggtcaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13508_sequence/1
type: Sequence
persistent identity: BBa M13508 sequence
display id: BBa_M13508_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatggaaacttcctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13110_sequence/1
type: Sequence
persistent identity: BBa M13110 sequence
display id: BBa_M13110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctttttgatgcaatccgctttgcttctgactataatagtcagggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13105_sequence/1
type: Sequence
persistent identity: BBa M13105 sequence
display id: BBa_M13105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13108_sequence/1
type: Sequence
persistent identity: BBa M13108 sequence
display id: BBa_M13108_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatctccgttgtactttgtttcgcgcttggtataatcgctgggggtc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13103_sequence/1
type: Sequence
persistent identity: BBa M13103 sequence
display id: BBa_M13103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattcacctcgaaagcaagctgataaaccgatacaattaaaggctcct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31742_sequence/1
type: Sequence
persistent identity: BBa M31742 sequence
display id: BBa_M31742_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctcttcgacgcaatccgcttcgcctccgactataatagccaagggaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcattcgaaggtgactctatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31744_sequence/1
type: Sequence
persistent identity: BBa M31744 sequence
display id: BBa_M31744_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccagcgtccagattggtataacgagcccgttctcaaaattgcatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31746_sequence/1
type: Sequence
persistent identity: BBa M31746 sequence
display id: BBa_M31746_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgtcccagccaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13503_sequence/1
type: Sequence
persistent identity: BBa M13503 sequence
display id: BBa_M13503_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttggagattttcaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31748_sequence/1
type: Sequence
persistent identity: BBa M31748 sequence
display id: BBa_M31748_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatatctgtcgtgctctgcttcgcactcggtattatcgccggtgggcagagatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31780_sequence/1
type: Sequence
persistent identity: BBa M31780 sequence
display id: BBa_M31780_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttgatggagacatcttcatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31782_sequence/1
type: Sequence
persistent identity: BBa M31782 sequence
display id: BBa_M31782_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaatttacatccaaggctagttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31784_sequence/1
type: Sequence
persistent identity: BBa M31784 sequence
display id: BBa_M31784_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataaatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaatttacatccaaggctagttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0082_sequence/1
type: Sequence
persistent identity: BBa R0082 sequence
display id: BBa_R0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31786_sequence/1
type: Sequence
persistent identity: BBa M31786 sequence
display id: BBa_M31786_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccgcctggtactgagcaaaaccccgctaatcctaatccttctcttgaggagtctcagcctcttaatactttcatgtttcagaataataggttccgaaataggcagggggcattaactgtttatacgggcactgttactcaaggcactgaccccgttaaaacttattaccagtacactcctgtatcatcaaaagccatgtatgacgcttactggaacggtaaattcagagactgcgctttccattctggctttaatgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z