BBa_M31971
type: ComponentDefinition
persistent identity: BBa M31971
display id: BBa_M31971
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 modified, from HpaI to BamHI site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: genomic sequence with modifications
owned by: james
owned by: myers
mutable description: modified by Rebecca Adams to separate genes, promoters, and RBS's in order to make refactoring easier.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_M13508
component: BBa_M13108
component: BBa_M31965
component: BBa_M31964
component: BBa_M31963
component: BBa_M13509
component: BBa_M31962
component: BBa_M31961
component: BBa_M31960
component: BBa_M13507
component: BBa_M31959
component: BBa_M31958
component: BBa_M31957
component: BBa_M13505
component: BBa_M13105
component: BBa_M31956
component: BBa_M31969
component: BBa_M13503
component: BBa_M13103
component: BBa_M31968
component: BBa_M31967
component: BBa_M31966
component: BBa_M31955
component: BBa_M31954
component: BBa_M13510
component: BBa_M13110
component: BBa_M31953
component: BBa_M31952
component: BBa_M31951
component: BBa_M31950
sequence annotation: BBa_M31950
sequence annotation: BBa_M31951
sequence annotation: BBa_M31952
sequence annotation: BBa_M31953
sequence annotation: BBa_M13110
sequence annotation: BBa_M13510
sequence annotation: BBa_M31954
sequence annotation: BBa_M31955
sequence annotation: BBa_M31956
sequence annotation: BBa_M13105
sequence annotation: BBa_M13505
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sequence annotation: BBa_M31959
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sequence annotation: BBa_M31961
sequence annotation: BBa_M31962
sequence annotation: BBa_M13509
sequence annotation: BBa_M31963
sequence annotation: BBa_M31964
sequence annotation: BBa_M31965

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end: 2430
orientation: inline

BBa_M31969
type: SequenceAnnotation
persistent identity: annotation1917031
display id: annotation1917031
version: 1
top level: BBa_M31971
location: https://synbiohub.org/public/igem/BBa_M31971/annotation1917031/range1917031/1
component: BBa_M31969

https://synbiohub.org/public/igem/BBa_M31971/annotation1917031/range1917031/1
type: Range
persistent identity: range1917031
display id: range1917031
version: 1
top level: BBa_M31971
direction: direction/reverse
start: 2439
end: 3084
orientation: inline

BBa_M13505
type: ComponentDefinition
persistent identity: BBa M13505
display id: BBa_M13505
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene V RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene V message (BBa_M13005). The part aligns with base pairs 827-842 in M13K07 genome. It was identified as the RBS for the gene V message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13505_sequence/1

BBa_M13507
type: ComponentDefinition
persistent identity: BBa M13507
display id: BBa_M13507
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene VII RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VII message (BBa_M13007). The part aligns with base pairs 1092-1107 in M13K07 genome. It was identified as the RBS for gene VII message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13507_sequence/1

BBa_M13509
type: ComponentDefinition
persistent identity: BBa M13509
display id: BBa_M13509
version: 1
was generated by: iGEM to SBOL conversion
description: M13Ko7 gene IX RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene IX message (BBa_M13009). The part aligns with base pairs 1190-1205 in M13K07 genome. It was identified as the RBS for gene IX message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13509_sequence/1

BBa_M13508
type: ComponentDefinition
persistent identity: BBa M13508
display id: BBa_M13508
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene VIII RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VIII message (BBa_M13008). The part aligns with base pairs 1285-1300 in M13K07 genome. It was identified as the RBS for gene VIII message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13508_sequence/1

BBa_M13105
type: ComponentDefinition
persistent identity: BBa M13105
display id: BBa_M13105
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene V promoter
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 786-835 in M13K07. It directs transcription of M13 gene V (BBa_M13505, BBa_M13005). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13105_sequence/1

BBa_M13108
type: ComponentDefinition
persistent identity: BBa M13108
display id: BBa_M13108
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene VIII promoter
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 1155-1201 in M13K07. It directs transcription of M13 gene VIII (BBa_M13508, BBa_M13008). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13108_sequence/1

AfeI
type: ComponentDefinition
persistent identity: BBa M31956
display id: BBa_M31956
version: 1
was generated by: iGEM to SBOL conversion
description: AfeI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: Restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31956_sequence/1

BBa_M31957
type: ComponentDefinition
persistent identity: BBa M31957
display id: BBa_M31957
version: 1
was generated by: iGEM to SBOL conversion
description: Gene V of M13K07, modified
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: genomic sequence
owned by: james
owned by: myers
mutable description: modified to reduce repeats, no AA changes
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31957_sequence/1

AseI
type: ComponentDefinition
persistent identity: BBa M31958
display id: BBa_M31958
version: 1
was generated by: iGEM to SBOL conversion
description: AseI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: Restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31958_sequence/1

AvrII
type: ComponentDefinition
persistent identity: BBa M31959
display id: BBa_M31959
version: 1
was generated by: iGEM to SBOL conversion
description: AvrII restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: Restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31959_sequence/1

BBa_M31960
type: ComponentDefinition
persistent identity: BBa M31960
display id: BBa_M31960
version: 1
was generated by: iGEM to SBOL conversion
description: Gene VII of M13K07 modified
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: sequence/modified
owned by: james
owned by: myers
mutable description: Modified to reduce repeats, same AA's present
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31960_sequence/1

BbvCI
type: ComponentDefinition
persistent identity: BBa M31961
display id: BBa_M31961
version: 1
was generated by: iGEM to SBOL conversion
description: BbvCI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31961_sequence/1

BccI
type: ComponentDefinition
persistent identity: BBa M31962
display id: BBa_M31962
version: 1
was generated by: iGEM to SBOL conversion
description: BccI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31962_sequence/1

BBa_M31963
type: ComponentDefinition
persistent identity: BBa M31963
display id: BBa_M31963
version: 1
was generated by: iGEM to SBOL conversion
description: Gene IX, modified
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: modified
owned by: james
owned by: myers
mutable description: M13K07, modified
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31963_sequence/1

BciVI
type: ComponentDefinition
persistent identity: BBa M31964
display id: BBa_M31964
version: 1
was generated by: iGEM to SBOL conversion
description: BciVI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_M31964_sequence/1

BclI
type: ComponentDefinition
persistent identity: BBa M31965
display id: BBa_M31965
version: 1
was generated by: iGEM to SBOL conversion
description: BclI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: restriction site
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_M31965_sequence/1

BBa_M13510
type: ComponentDefinition
persistent identity: BBa M13510
display id: BBa_M13510
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene X RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene X message (BBa_M13010). The part aligns with base pairs 480-495 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13510_sequence/1

BBa_M13110
type: ComponentDefinition
persistent identity: BBa M13110
display id: BBa_M13110
version: 1
was generated by: iGEM to SBOL conversion
description: M13110
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 381-428 in M13K07. It directs transcription of M13 gene X (BBa_M13510, BBa_M13010). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13110_sequence/1

BBa_M13103
type: ComponentDefinition
persistent identity: BBa M13103
display id: BBa_M13103
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene III promoter
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 1500-1547 in M13K07. It directs transcription of M13 gene III (BBa_M13503, BBa_M13003). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_M13103_sequence/1

HpaI
type: ComponentDefinition
persistent identity: BBa M31950
display id: BBa_M31950
version: 1
was generated by: iGEM to SBOL conversion
description: HpaI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: standard, restriction site
owned by: james
owned by: myers
mutable description: This is a restriction site.
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31950_sequence/1

BBa_M31951
type: ComponentDefinition
persistent identity: BBa M31951
display id: BBa_M31951
version: 1
was generated by: iGEM to SBOL conversion
description: part of Gene II of M13K07
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: genomic sequence
owned by: james
owned by: myers
mutable description: Last part of Gene II, after the HpaI
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31951_sequence/1

AatII
type: ComponentDefinition
persistent identity: BBa M31952
display id: BBa_M31952
version: 1
was generated by: iGEM to SBOL conversion
description: AatII restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known it sequence
owned by: james
owned by: myers
mutable description: restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31952_sequence/1

Acc65I
type: ComponentDefinition
persistent identity: BBa M31953
display id: BBa_M31953
version: 1
was generated by: iGEM to SBOL conversion
description: Acc65I restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: Restriction site
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31953_sequence/1

BBa_M31954
type: ComponentDefinition
persistent identity: BBa M31954
display id: BBa_M31954
version: 1
was generated by: iGEM to SBOL conversion
description: Gene X, M13K07 modified
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: genomic sequence
owned by: james
owned by: myers
mutable description: Entire gene with modifications due to overlapps
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: unknown/none
star: false
creator: Rebecca Adams
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_M31954_sequence/1

ApaI
type: ComponentDefinition
persistent identity: BBa M31955
display id: BBa_M31955
version: 1
was generated by: iGEM to SBOL conversion
description: ApaI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: Known cut site
owned by: james
owned by: myers
mutable description: Restriction site
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_M31955_sequence/1

BBa_M31966
type: ComponentDefinition
persistent identity: BBa M31966
display id: BBa_M31966
version: 1
was generated by: iGEM to SBOL conversion
description: Gene VIII, M13K07 modified
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: modified genomic
owned by: james
owned by: myers
mutable description: modified, same AA sequence
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31966_sequence/1

BfaI
type: ComponentDefinition
persistent identity: BBa M31967
display id: BBa_M31967
version: 1
was generated by: iGEM to SBOL conversion
description: BfaI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: restriction site for BfaI
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31967_sequence/1

BfuAI
type: ComponentDefinition
persistent identity: BBa M31968
display id: BBa_M31968
version: 1
was generated by: iGEM to SBOL conversion
description: BfuAI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31968_sequence/1

BBa_M31969
type: ComponentDefinition
persistent identity: BBa M31969
display id: BBa_M31969
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 Gene III, truncated
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence, genomic
owned by: james
owned by: myers
mutable description: Stopped after Bam HI site
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31969_sequence/1

BBa_M13503
type: ComponentDefinition
persistent identity: BBa M13503
display id: BBa_M13503
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene III RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene III message (BBa_M13003). The part aligns with base pairs 1563-1578 in M13K07 genome. It was identified as the RBS for gene III message by Wezenbeek et al in Gene (1980) 11: 129-148 New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13503_sequence/1

https://synbiohub.org/public/igem/BBa_M31971_sequence/1
type: Sequence
persistent identity: BBa M31971 sequence
display id: BBa_M31971_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttactagagaacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaacctctttgacgctatccgtttcgcatcagattacaacagtcaaggaaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcattcgaaggtgactcgatgaacatctacgatgactcagccgttttagatgctattcaatcaaaacactttacaatcaccccatcaggcaagacatctttcgccaaagcatcacgctacttcggtttctaccgtcgactcgtaaatgatggttacgacagcgttgcactcactatgccccgtaattctttctggcgatacgtatcagctttagtcgagtgtggaatccctaagtcccaactaatgaacctttccacttgcaacaatgtcgtaccattggtccgattcattaatgtggacttttcatctcaacgccctgattggtacaatgaaccagtccttaagatcgcgtaatactagaggacgtctactagagggtacctactagagtctttttgatgcaatccgctttgcttctgactataatagtcagggtaatactagagatttgagggggattcatactagatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataatactagaggggccctactagagagcgcttactagagccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtatactagagcataaggtaattcacatactagatgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgtcccagctaaatagtactagagattaattactagagcctaggtactagaggttccggctaagtaactactagatggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatttcagtcgtgctttgctttgcgctcggtatcattgctggaggacagagatgatactagagcctcagctactagagccatctactagagtcgctgggggtcaaagtactagatgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttgatggagacctcttcatgatactagaggta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13505_sequence/1
type: Sequence
persistent identity: BBa M13505 sequence
display id: BBa_M13505_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cataaggtaattcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13507_sequence/1
type: Sequence
persistent identity: BBa M13507 sequence
display id: BBa_M13507_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttccggctaagtaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13509_sequence/1
type: Sequence
persistent identity: BBa M13509 sequence
display id: BBa_M13509_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcgctgggggtcaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13508_sequence/1
type: Sequence
persistent identity: BBa M13508 sequence
display id: BBa_M13508_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatggaaacttcctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13105_sequence/1
type: Sequence
persistent identity: BBa M13105 sequence
display id: BBa_M13105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13108_sequence/1
type: Sequence
persistent identity: BBa M13108 sequence
display id: BBa_M13108_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatctccgttgtactttgtttcgcgcttggtataatcgctgggggtc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31956_sequence/1
type: Sequence
persistent identity: BBa M31956 sequence
display id: BBa_M31956_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcgct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31957_sequence/1
type: Sequence
persistent identity: BBa M31957 sequence
display id: BBa_M31957_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgtcccagctaaatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31958_sequence/1
type: Sequence
persistent identity: BBa M31958 sequence
display id: BBa_M31958_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31959_sequence/1
type: Sequence
persistent identity: BBa M31959 sequence
display id: BBa_M31959_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31960_sequence/1
type: Sequence
persistent identity: BBa M31960 sequence
display id: BBa_M31960_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatttcagtcgtgctttgctttgcgctcggtatcattgctggaggacagagatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31961_sequence/1
type: Sequence
persistent identity: BBa M31961 sequence
display id: BBa_M31961_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31962_sequence/1
type: Sequence
persistent identity: BBa M31962 sequence
display id: BBa_M31962_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31963_sequence/1
type: Sequence
persistent identity: BBa M31963 sequence
display id: BBa_M31963_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttgatggagacctcttcatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31964_sequence/1
type: Sequence
persistent identity: BBa M31964 sequence
display id: BBa_M31964_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtatcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31965_sequence/1
type: Sequence
persistent identity: BBa M31965 sequence
display id: BBa_M31965_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgatca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13510_sequence/1
type: Sequence
persistent identity: BBa M13510 sequence
display id: BBa_M13510_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atttgagggggattca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13503_sequence/1
type: Sequence
persistent identity: BBa M13503 sequence
display id: BBa_M13503_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttggagattttcaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13110_sequence/1
type: Sequence
persistent identity: BBa M13110 sequence
display id: BBa_M13110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctttttgatgcaatccgctttgcttctgactataatagtcagggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13103_sequence/1
type: Sequence
persistent identity: BBa M13103 sequence
display id: BBa_M13103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattcacctcgaaagcaagctgataaaccgatacaattaaaggctcct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31950_sequence/1
type: Sequence
persistent identity: BBa M31950 sequence
display id: BBa_M31950_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31951_sequence/1
type: Sequence
persistent identity: BBa M31951 sequence
display id: BBa_M31951_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaacctctttgacgctatccgtttcgcatcagattacaacagtcaaggaaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcattcgaaggtgactcgatgaacatctacgatgactcagccgttttagatgctattcaatcaaaacactttacaatcaccccatcaggcaagacatctttcgccaaagcatcacgctacttcggtttctaccgtcgactcgtaaatgatggttacgacagcgttgcactcactatgccccgtaattctttctggcgatacgtatcagctttagtcgagtgtggaatccctaagtcccaactaatgaacctttccacttgcaacaatgtcgtaccattggtccgattcattaatgtggacttttcatctcaacgccctgattggtacaatgaaccagtccttaagatcgcgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31952_sequence/1
type: Sequence
persistent identity: BBa M31952 sequence
display id: BBa_M31952_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacgtc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31953_sequence/1
type: Sequence
persistent identity: BBa M31953 sequence
display id: BBa_M31953_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31954_sequence/1
type: Sequence
persistent identity: BBa M31954 sequence
display id: BBa_M31954_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31955_sequence/1
type: Sequence
persistent identity: BBa M31955 sequence
display id: BBa_M31955_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31966_sequence/1
type: Sequence
persistent identity: BBa M31966 sequence
display id: BBa_M31966_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaagtttacctcaaaggctagttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31967_sequence/1
type: Sequence
persistent identity: BBa M31967 sequence
display id: BBa_M31967_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31968_sequence/1
type: Sequence
persistent identity: BBa M31968 sequence
display id: BBa_M31968_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31969_sequence/1
type: Sequence
persistent identity: BBa M31969 sequence
display id: BBa_M31969_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccgcctggtactgagcaaaaccccgctaatcctaatccttctcttgaggagtctcagcctcttaatactttcatgtttcagaataataggttccgaaataggcagggggcattaactgtttatacgggcactgttactcaaggcactgaccccgttaaaacttattaccagtacactcctgtatcatcaaaagccatgtatgacgcttactggaacggtaaattcagagactgcgctttccattctggctttaatgaggatc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z