BBa_M31975
type: ComponentDefinition
persistent identity: BBa M31975
display id: BBa_M31975
version: 1
was generated by: iGEM to SBOL conversion
description: M13 refactoring
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: parts from M13K07 genome
owned by: james
owned by: myers
mutable description: M13K07 refactored to elimiate overlaps and add restriction enzyme sites inbetween parts.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: 1) eliminate rbs and promoters within genes: took into consideration codon changes that would not change amino acid sequences but would decrease the strength of the rbs or promoter. 2) add restriction sites between each part: tried to keep sticky ends as consistent as possible (i.e. after every promoter, the restriction site sticky end is 3'ctag), however, there aren't enough zero cutting isoschizomers.
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_M31980
component: BBa_M31988
component: BBa_M13507
component: BBa_M31995
component: BBa_M31978
component: BBa_M31986
component: BBa_M13509
component: BBa_M31987
component: BBa_M13508
component: BBa_M31993
component: BBa_M13108
component: BBa_M31985
component: BBa_M31977
component: BBa_M31983
component: BBa_M13008
component: BBa_M31984
component: BBa_M13002
component: BBa_M13510
component: BBa_M31994
component: BBa_M13110
component: BBa_M31997
component: BBa_M13105
component: BBa_M31996
component: BBa_M13010
component: BBa_M31990
component: BBa_M31981
component: BBa_M31989
component: BBa_M13505
component: BBa_M31992
component: BBa_M13503
component: BBa_M31991
component: BBa_M13103
component: BBa_M31976
component: BBa_M13003
component: BBa_M31982
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sequence annotation: BBa_M31997
sequence annotation: BBa_M13110
sequence annotation: BBa_M31994
sequence annotation: BBa_M13510
sequence annotation: BBa_M31990
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sequence annotation: BBa_M31996
sequence annotation: BBa_M13105
sequence annotation: BBa_M31992
sequence annotation: BBa_M13505
sequence annotation: BBa_M31989
sequence annotation: BBa_M31981
sequence annotation: BBa_M31995
sequence annotation: BBa_M13507
sequence annotation: BBa_M31988
sequence annotation: BBa_M31980

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orientation: inline

BBa_M31983
type: SequenceAnnotation
persistent identity: annotation1917530
display id: annotation1917530
version: 1
top level: BBa_M31975
location: https://synbiohub.org/public/igem/BBa_M31975/annotation1917530/range1917530/1
component: BBa_M31983

https://synbiohub.org/public/igem/BBa_M31975/annotation1917530/range1917530/1
type: Range
persistent identity: range1917530
display id: range1917530
version: 1
top level: BBa_M31975
direction: direction/reverse
start: 2562
end: 2567
orientation: inline

BBa_M31977
type: SequenceAnnotation
persistent identity: annotation1917531
display id: annotation1917531
version: 1
top level: BBa_M31975
location: https://synbiohub.org/public/igem/BBa_M31975/annotation1917531/range1917531/1
component: BBa_M31977

https://synbiohub.org/public/igem/BBa_M31975/annotation1917531/range1917531/1
type: Range
persistent identity: range1917531
display id: range1917531
version: 1
top level: BBa_M31975
direction: direction/reverse
start: 2568
end: 2601
orientation: inline

BBa_M31976
type: SequenceAnnotation
persistent identity: annotation1917532
display id: annotation1917532
version: 1
top level: BBa_M31975
location: https://synbiohub.org/public/igem/BBa_M31975/annotation1917532/range1917532/1
component: BBa_M31976

https://synbiohub.org/public/igem/BBa_M31975/annotation1917532/range1917532/1
type: Range
persistent identity: range1917532
display id: range1917532
version: 1
top level: BBa_M31975
direction: direction/reverse
start: 2602
end: 2607
orientation: inline

BBa_M13103
type: SequenceAnnotation
persistent identity: annotation1917533
display id: annotation1917533
version: 1
top level: BBa_M31975
location: https://synbiohub.org/public/igem/BBa_M31975/annotation1917533/range1917533/1
component: BBa_M13103

https://synbiohub.org/public/igem/BBa_M31975/annotation1917533/range1917533/1
type: Range
persistent identity: range1917533
display id: range1917533
version: 1
top level: BBa_M31975
direction: direction/reverse
start: 2608
end: 2655
orientation: inline

BBa_M31991
type: SequenceAnnotation
persistent identity: annotation1917534
display id: annotation1917534
version: 1
top level: BBa_M31975
location: https://synbiohub.org/public/igem/BBa_M31975/annotation1917534/range1917534/1
component: BBa_M31991

https://synbiohub.org/public/igem/BBa_M31975/annotation1917534/range1917534/1
type: Range
persistent identity: range1917534
display id: range1917534
version: 1
top level: BBa_M31975
direction: direction/reverse
start: 2656
end: 2661
orientation: inline

BBa_M13503
type: SequenceAnnotation
persistent identity: annotation1917535
display id: annotation1917535
version: 1
top level: BBa_M31975
location: https://synbiohub.org/public/igem/BBa_M31975/annotation1917535/range1917535/1
component: BBa_M13503

https://synbiohub.org/public/igem/BBa_M31975/annotation1917535/range1917535/1
type: Range
persistent identity: range1917535
display id: range1917535
version: 1
top level: BBa_M31975
direction: direction/reverse
start: 2662
end: 2677
orientation: inline

BBa_M31982
type: SequenceAnnotation
persistent identity: annotation1917536
display id: annotation1917536
version: 1
top level: BBa_M31975
location: https://synbiohub.org/public/igem/BBa_M31975/annotation1917536/range1917536/1
component: BBa_M31982

https://synbiohub.org/public/igem/BBa_M31975/annotation1917536/range1917536/1
type: Range
persistent identity: range1917536
display id: range1917536
version: 1
top level: BBa_M31975
direction: direction/reverse
start: 2678
end: 2685
orientation: inline

BBa_M13003
type: SequenceAnnotation
persistent identity: annotation1917537
display id: annotation1917537
version: 1
top level: BBa_M31975
location: https://synbiohub.org/public/igem/BBa_M31975/annotation1917537/range1917537/1
component: BBa_M13003

https://synbiohub.org/public/igem/BBa_M31975/annotation1917537/range1917537/1
type: Range
persistent identity: range1917537
display id: range1917537
version: 1
top level: BBa_M31975
direction: direction/reverse
start: 2686
end: 3960
orientation: inline

BBa_M13002
type: ComponentDefinition
persistent identity: BBa M13002
display id: BBa_M13002
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene II
created: 2006-12-13T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome. It encodes a protein that is important for phage DNA replication, initiating ssDNA synthesis of the (+) strand by nicking the RF near the M13 replication origin. p10 is encoded within gII thanks to a translation re-initiation event.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: need to unstuff gX from within coding sequence. RBS for downstream gV overlaps the gII stop codon.
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M13002_sequence/1

BBa_M13507
type: ComponentDefinition
persistent identity: BBa M13507
display id: BBa_M13507
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene VII RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VII message (BBa_M13007). The part aligns with base pairs 1092-1107 in M13K07 genome. It was identified as the RBS for gene VII message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13507_sequence/1

BsrG1
type: ComponentDefinition
persistent identity: BBa M31995
display id: BBa_M31995
version: 1
was generated by: iGEM to SBOL conversion
description: BsrGI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: Bacillus stearothermophilus GR75 (Z. Chen)
owned by: james
owned by: myers
mutable description: BsrGI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31995_sequence/1

MluI
type: ComponentDefinition
persistent identity: BBa M31988
display id: BBa_M31988
version: 1
was generated by: iGEM to SBOL conversion
description: MluI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: MluI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_M31988_sequence/1

BclI
type: ComponentDefinition
persistent identity: BBa M31987
display id: BBa_M31987
version: 1
was generated by: iGEM to SBOL conversion
description: BclI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: BclI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31987_sequence/1

BBa_M31980
type: ComponentDefinition
persistent identity: BBa M31980
display id: BBa_M31980
version: 1
was generated by: iGEM to SBOL conversion
description: gene 7 from M13K07 modified
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: M13K07 genome
owned by: james
owned by: myers
mutable description: gene 7 from M13K07 modified to remove rbs 9 and promoter of gene 8.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Tiffany Guo
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_M31980_sequence/1

BBa_M13010
type: ComponentDefinition
persistent identity: BBa M13010
display id: BBa_M13010
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene X
created: 2006-12-05T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome. It encodes a protein that is important for phage maturation. The protein remains in the host cytoplasm to interact with the product of gI and the OM pore (product of gIV) through which the phage ssDNA is threaded as the phage matures. GeneX protein is identical to the C-terminal portion of the gIprotein since gXprotien is normally encoded through an internal initiation of translation from the gI transcript.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: need to unstuff from inside gI
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M13010_sequence/1

BBa_M13008
type: ComponentDefinition
persistent identity: BBa M13008
display id: BBa_M13008
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene VIII
created: 2006-12-12T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome. It encodes the major coat protein, with approximately 2700 copies of the protein on each phage particle. The protein is first synthesized at a longer precursor protein that is clipped between the Alanines at positions 23 and 24. It is possible to silently add a Pst site to clone fusions upstream and in frame with Ala that begins mature protein. Small peptide-fusions are possible to the N-terminal portion of the mature p8 (phage display systems are built around this finding) but addition of more than 6 or 8 residues makes the phage pack poorly, leading to unproductive infections.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Many! The start codon for gene 8 overlaps with the stop codon for the upstream gene 9. Might be nice to include cloning sites for directed N-terminal fusions to mature form of protein. Might be nice to codon vary a different biobrick of gene VIII to allow for two genes expressed on single genome, inhibiting recombination in ssDNA.
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_M13008_sequence/1

BBa_M13510
type: ComponentDefinition
persistent identity: BBa M13510
display id: BBa_M13510
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene X RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene X message (BBa_M13010). The part aligns with base pairs 480-495 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13510_sequence/1

BBa_M13509
type: ComponentDefinition
persistent identity: BBa M13509
display id: BBa_M13509
version: 1
was generated by: iGEM to SBOL conversion
description: M13Ko7 gene IX RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene IX message (BBa_M13009). The part aligns with base pairs 1190-1205 in M13K07 genome. It was identified as the RBS for gene IX message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13509_sequence/1

BBa_M13508
type: ComponentDefinition
persistent identity: BBa M13508
display id: BBa_M13508
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene VIII RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VIII message (BBa_M13008). The part aligns with base pairs 1285-1300 in M13K07 genome. It was identified as the RBS for gene VIII message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13508_sequence/1

BBa_M13110
type: ComponentDefinition
persistent identity: BBa M13110
display id: BBa_M13110
version: 1
was generated by: iGEM to SBOL conversion
description: M13110
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 381-428 in M13K07. It directs transcription of M13 gene X (BBa_M13510, BBa_M13010). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13110_sequence/1

BBa_M13108
type: ComponentDefinition
persistent identity: BBa M13108
display id: BBa_M13108
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene VIII promoter
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 1155-1201 in M13K07. It directs transcription of M13 gene VIII (BBa_M13508, BBa_M13008). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13108_sequence/1

Acc651
type: ComponentDefinition
persistent identity: BBa M31997
display id: BBa_M31997
version: 1
was generated by: iGEM to SBOL conversion
description: Acc65I restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: An E. coli strain that carries the cloned Acc65I gene from Acinetobacter calcoaceticus 65
owned by: james
owned by: myers
mutable description: Acc65I restriction enzyme site
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_M31997_sequence/1

BsiWI
type: ComponentDefinition
persistent identity: BBa M31996
display id: BBa_M31996
version: 1
was generated by: iGEM to SBOL conversion
description: BsiWI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: A E. coli strain that carries the BsiWI gene from Bacillus species (D. Clark).
owned by: james
owned by: myers
mutable description: BsiWI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_M31996_sequence/1

AvrII
type: ComponentDefinition
persistent identity: BBa M31994
display id: BBa_M31994
version: 1
was generated by: iGEM to SBOL conversion
description: AvrII restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: A E. coli strain that carries the AvrII gene from Anabaena variabilis UW (E. Rosenvold).
owned by: james
owned by: myers
mutable description: AvrII restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31994_sequence/1

NheI
type: ComponentDefinition
persistent identity: BBa M31993
display id: BBa_M31993
version: 1
was generated by: iGEM to SBOL conversion
description: NheI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: A E. coli strain that carries the NheI gene from Neisseria mucosa heidelbergensis (ATCC 25999).
owned by: james
owned by: myers
mutable description: NheI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31993_sequence/1

AscI
type: ComponentDefinition
persistent identity: BBa M31990
display id: BBa_M31990
version: 1
was generated by: iGEM to SBOL conversion
description: AscI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: AscI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_M31990_sequence/1

EagI
type: ComponentDefinition
persistent identity: BBa M31986
display id: BBa_M31986
version: 1
was generated by: iGEM to SBOL conversion
description: EagI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: EagI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31986_sequence/1

PspOMI
type: ComponentDefinition
persistent identity: BBa M31984
display id: BBa_M31984
version: 1
was generated by: iGEM to SBOL conversion
description: PspOMI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: PspOMI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31984_sequence/1

BBa_M31978
type: ComponentDefinition
persistent identity: BBa M31978
display id: BBa_M31978
version: 1
was generated by: iGEM to SBOL conversion
description: gene 9 from M13K07 modified
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: M13K07 genome
owned by: james
owned by: myers
mutable description: gene 9 from M13K07 modified to remove rbs 8.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M31978_sequence/1

BBa_M31977
type: ComponentDefinition
persistent identity: BBa M31977
display id: BBa_M31977
version: 1
was generated by: iGEM to SBOL conversion
description: transcription terminator
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: M13K07 genome
owned by: james
owned by: myers
mutable description: transcription terminator (hairpin)
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence: https://synbiohub.org/public/igem/BBa_M31977_sequence/1

BglII
type: ComponentDefinition
persistent identity: BBa M31985
display id: BBa_M31985
version: 1
was generated by: iGEM to SBOL conversion
description: BglII restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: BglII restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_M31985_sequence/1

EcoRI
type: ComponentDefinition
persistent identity: BBa M31983
display id: BBa_M31983
version: 1
was generated by: iGEM to SBOL conversion
description: EcoRI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: EcoRI restriction enzyme site
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31983_sequence/1

BBa_M13003
type: ComponentDefinition
persistent identity: BBa M13003
display id: BBa_M13003
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene III
created: 2006-12-13T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This gene is part of the bacteriophage M13 genome, encoding a minor coat protein. Approximately 5 copies of p3 are presented with 5 copies of p6 on the rounded end of the mature phage. In the absence of p3, the phage remains tethered to the bacterial host, elongating to 10 or 20 times the normal phage length and incorporating multiple ssDNA genomes within the coat. p3 (domain II) is also responsible for the initial interaction of the phage with the bacterial pili during infection. Peptide fusions, sometimes large, can be displayed on N-terminal end of p3, though highly charged fusions are difficult to isolate since they are not easily secreted by the host secretion apparatus. The p3 protein is first synthesized as a longer precursor protein that is 18 amino acids longer than the mature protein. The gene's translation initiates with a GTG codon, and changing this codon to an ATG increases expression of gene III to levels with negative effects on phage maturation.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The RBS for gene III is within the transcription termination sequence of the upstream gVIII, thus modifications to termination affect gIII initiation. Translation initiates with a GTG rather than a canonical ATG. There is a unique, natural BamHI site in domain II that may be useful for cloning, though it may prevent interaction of p3 with pilus.
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_M13003_sequence/1

BBa_M13505
type: ComponentDefinition
persistent identity: BBa M13505
display id: BBa_M13505
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene V RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene V message (BBa_M13005). The part aligns with base pairs 827-842 in M13K07 genome. It was identified as the RBS for the gene V message by Wezenbeek et al in Gene (1980) 11: 129-148
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13505_sequence/1

BBa_M13503
type: ComponentDefinition
persistent identity: BBa M13503
display id: BBa_M13503
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene III RBS
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene III message (BBa_M13003). The part aligns with base pairs 1563-1578 in M13K07 genome. It was identified as the RBS for gene III message by Wezenbeek et al in Gene (1980) 11: 129-148 New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_M13503_sequence/1

BBa_M13105
type: ComponentDefinition
persistent identity: BBa M13105
display id: BBa_M13105
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene V promoter
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 786-835 in M13K07. It directs transcription of M13 gene V (BBa_M13505, BBa_M13005). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13105_sequence/1

BBa_M13103
type: ComponentDefinition
persistent identity: BBa M13103
display id: BBa_M13103
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene III promoter
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
mutable description: This part is from the bacteriophage M13 genome, bp 1500-1547 in M13K07. It directs transcription of M13 gene III (BBa_M13503, BBa_M13003). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_M13103_sequence/1

XbaI
type: ComponentDefinition
persistent identity: BBa M31991
display id: BBa_M31991
version: 1
was generated by: iGEM to SBOL conversion
description: XbaI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: XbaI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_M31991_sequence/1

BssHII
type: ComponentDefinition
persistent identity: BBa M31989
display id: BBa_M31989
version: 1
was generated by: iGEM to SBOL conversion
description: BssHII restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: BssHII restriction enzyme site
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_M31989_sequence/1

NotI
type: ComponentDefinition
persistent identity: BBa M31982
display id: BBa_M31982
version: 1
was generated by: iGEM to SBOL conversion
description: NotI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: NotI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_M31982_sequence/1

BBa_M31981
type: ComponentDefinition
persistent identity: BBa M31981
display id: BBa_M31981
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene 5 modified
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: M13K07 genome
owned by: james
owned by: myers
mutable description: Modified to remove rbs 7 and restriction site for BsrGI.
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: keep amino acids the same, change codons.
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M31981_sequence/1

BBa_M31976
type: ComponentDefinition
persistent identity: BBa M31976
display id: BBa_M31976
version: 1
was generated by: iGEM to SBOL conversion
description: MfeI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: MfeI restriction enzyme site
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31976_sequence/1

SpeI
type: ComponentDefinition
persistent identity: BBa M31992
display id: BBa_M31992
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: SpeI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31992_sequence/1

https://synbiohub.org/public/igem/BBa_M31975_sequence/1
type: Sequence
persistent identity: BBa M31975 sequence
display id: BBa_M31975_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattgacatgctagttttacgattaccgttcatcgattctcttgtttgctccagactctcaggcaatgacctgatagcctttgtagacctctcaaaaatagctaccctctccggcatgaatttatcagctagaacggttgaatatcatattgatggtgatttgactgtctccggcctttctcacccttttgaatctttacctacacattactcaggcattgcatttaaaatatatgagggttctaaaaatttttatccttgcgttgaaataaaggcttctcccgcaaaagtattacagggtcataatgtttttggtacaaccgatttagctttatgctctgaggctttattgcttaattttgctaattctttgccttgcctgtatgatttattggatgttaacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtacctctttttgatgcaatccgctttgcttctgactataatagtcagggtaacctaggatttgagggggattcaggcgcgccatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataacgtacgccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtaactagtcataaggtaattcacagcgcgcatgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtataccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgttccagctaacta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13010_sequence/1
type: Sequence
persistent identity: BBa M13010 sequence
display id: BBa_M13010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13008_sequence/1
type: Sequence
persistent identity: BBa M13008 sequence
display id: BBa_M13008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattcacctcgaaagcaagctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13002_sequence/1
type: Sequence
persistent identity: BBa M13002 sequence
display id: BBa_M13002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattgacatgctagttttacgattaccgttcatcgattctcttgtttgctccagactctcaggcaatgacctgatagcctttgtagacctctcaaaaatagctaccctctccggcatgaatttatcagctagaacggttgaatatcatattgatggtgatttgactgtctccggcctttctcacccttttgaatctttacctacacattactcaggcattgcatttaaaatatatgagggttctaaaaatttttatccttgcgttgaaataaaggcttctcccgcaaaagtattacagggtcataatgtttttggtacaaccgatttagctttatgctctgaggctttattgcttaattttgctaattctttgccttgcctgtatgatttattggatgttaacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13510_sequence/1
type: Sequence
persistent identity: BBa M13510 sequence
display id: BBa_M13510_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atttgagggggattca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13507_sequence/1
type: Sequence
persistent identity: BBa M13507 sequence
display id: BBa_M13507_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttccggctaagtaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13509_sequence/1
type: Sequence
persistent identity: BBa M13509 sequence
display id: BBa_M13509_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcgctgggggtcaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13508_sequence/1
type: Sequence
persistent identity: BBa M13508 sequence
display id: BBa_M13508_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatggaaacttcctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13110_sequence/1
type: Sequence
persistent identity: BBa M13110 sequence
display id: BBa_M13110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctttttgatgcaatccgctttgcttctgactataatagtcagggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13108_sequence/1
type: Sequence
persistent identity: BBa M13108 sequence
display id: BBa_M13108_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatctccgttgtactttgtttcgcgcttggtataatcgctgggggtc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31997_sequence/1
type: Sequence
persistent identity: BBa M31997 sequence
display id: BBa_M31997_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31996_sequence/1
type: Sequence
persistent identity: BBa M31996 sequence
display id: BBa_M31996_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtacg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31995_sequence/1
type: Sequence
persistent identity: BBa M31995 sequence
display id: BBa_M31995_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31994_sequence/1
type: Sequence
persistent identity: BBa M31994 sequence
display id: BBa_M31994_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31988_sequence/1
type: Sequence
persistent identity: BBa M31988 sequence
display id: BBa_M31988_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acgcgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31987_sequence/1
type: Sequence
persistent identity: BBa M31987 sequence
display id: BBa_M31987_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgatca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31980_sequence/1
type: Sequence
persistent identity: BBa M31980 sequence
display id: BBa_M31980_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatctccgtagtactgtgtttcgcgctgggcattatcgctgggggccaaagatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13003_sequence/1
type: Sequence
persistent identity: BBa M13003 sequence
display id: BBa_M13003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccgcctggtactgagcaaaaccccgctaatcctaatccttctcttgaggagtctcagcctcttaatactttcatgtttcagaataataggttccgaaataggcagggggcattaactgtttatacgggcactgttactcaaggcactgaccccgttaaaacttattaccagtacactcctgtatcatcaaaagccatgtatgacgcttactggaacggtaaattcagagactgcgctttccattctggctttaatgaggatccattcgtttgtgaatatcaaggccaatcgtctgacctgcctcaacctcctgtcaatgctggcggcggctctggtggtggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctctgagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacgctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaaacttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggccttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcggtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatcggttgaatgtcgcccttttgtctttagcgctggtaaaccatatgaattttctattgattgtgacaaaataaacttattccgtggtgtctttgcgtttcttttatatgttgccacctttatgtatgtattttctacgtttgctaacatactgcgtaataaggagtcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13505_sequence/1
type: Sequence
persistent identity: BBa M13505 sequence
display id: BBa_M13505_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cataaggtaattcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13503_sequence/1
type: Sequence
persistent identity: BBa M13503 sequence
display id: BBa_M13503_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttggagattttcaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13105_sequence/1
type: Sequence
persistent identity: BBa M13105 sequence
display id: BBa_M13105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M13103_sequence/1
type: Sequence
persistent identity: BBa M13103 sequence
display id: BBa_M13103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattcacctcgaaagcaagctgataaaccgatacaattaaaggctcct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31993_sequence/1
type: Sequence
persistent identity: BBa M31993 sequence
display id: BBa_M31993_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31991_sequence/1
type: Sequence
persistent identity: BBa M31991 sequence
display id: BBa_M31991_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31990_sequence/1
type: Sequence
persistent identity: BBa M31990 sequence
display id: BBa_M31990_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcgcgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31989_sequence/1
type: Sequence
persistent identity: BBa M31989 sequence
display id: BBa_M31989_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31986_sequence/1
type: Sequence
persistent identity: BBa M31986 sequence
display id: BBa_M31986_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cggccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31984_sequence/1
type: Sequence
persistent identity: BBa M31984 sequence
display id: BBa_M31984_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31982_sequence/1
type: Sequence
persistent identity: BBa M31982 sequence
display id: BBa_M31982_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcggccgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31981_sequence/1
type: Sequence
persistent identity: BBa M31981 sequence
display id: BBa_M31981_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtataccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgttccagctaactaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31978_sequence/1
type: Sequence
persistent identity: BBa M31978 sequence
display id: BBa_M31978_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttaatggaaacttcatcatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31977_sequence/1
type: Sequence
persistent identity: BBa M31977 sequence
display id: BBa_M31977_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacaattaaaggctccttttggagcctttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31976_sequence/1
type: Sequence
persistent identity: BBa M31976 sequence
display id: BBa_M31976_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caattg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31985_sequence/1
type: Sequence
persistent identity: BBa M31985 sequence
display id: BBa_M31985_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agatct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31992_sequence/1
type: Sequence
persistent identity: BBa M31992 sequence
display id: BBa_M31992_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31983_sequence/1
type: Sequence
persistent identity: BBa M31983 sequence
display id: BBa_M31983_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z