BBa_M33002
1
BBa_M33002
CDS for Fnr-LH28 protein, activates narGp in presence of nitrate.
2010-05-31T11:00:00Z
2015-05-08T01:14:01Z
The part comes from the E. coli genome.
The fnr gene naturally is activated under anaerobic conditions during E. coli nitrogen respiration. The part above contains a single missense LH28 mutation to allow it to operate under aerobic conditions. Cytoplasmic Fnr-LH28 protein created by the gene can bind to a regulatory region upstream of the nar operon as an activator, facilitating the reduction of nitrate to nitrite.
false
false
_577_
0
6923
9
Not in stock
false
The coding sequence was checked for restriction enzyme sites to ensure that it is Assembly Standard 10-compliant.
false
Daniel Bui and Aaditya Shidham
annotation2069958
1
Stop Codon
range2069958
1
751
756
annotation2069957
1
T to A LH28 Mutation
range2069957
1
83
83
annotation2069956
1
Start Codon
range2069956
1
1
3
annotation2069955
1
fnr-LH28
range2069955
1
1
756
BBa_M33002_sequence
1
atgatcccggaaaagcgaattatacggcgcattcagtctggcggttgtgctatccattgccaggattgcagcatcagccagcattgcatcccgttcacactcaacgaacatgagcttgatcagcttgataatatcattgagcggaagaagcctattcagaaaggccagacgctgtttaaggctggtgatgaacttaaatcgctttatgccatccgctccggtacgattaaaagttataccatcactgagcaaggcgacgagcaaatcactggtttccatttagcaggcgacctggtgggatttgacgccatcggcagcggccatcacccgagcttcgcgcaggcgctggaaacctcgatggtatgtgaaatcccgttcgaaacgctggacgatttgtccggtaaaatgccgaatctgcgtcagcagatgatgcgtctgatgagcggtgaaatcaaaggcgatcaggacatgatcctgctgttgtcgaagaaaaatgccgaggaacgtctggctgcattcatctacaacctgtcccgtcgttttgcccaacgcggcttctcccctcgtgagttccgcctgacgatgactcgtggcgatatcggtaactatctgggcctgacggtagaaaccatcagccgtctgctgggtcgcttccagaaaagcggcatgctggcagtcaaaggtaaatacatcaccatcgaaaataacgatgcgctggcccagcttgctggtcatacgcgtaacgttgcctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z