BBa_M33330 1 BBa_M33330 RC of atzR CDS 2010-05-31T11:00:00Z 2015-05-08T01:14:01Z From Pseudomonas sp. ADP at http://www.ncbi.nlm.nih.gov/nuccore/13937422, bp 99885..100853. Formerly called orf99. Pseudomonas sp. ADP digests atrazine under nitrogen starvation conditions. atzABC are constitutively expressed and convert atrazine to cyanuric acid. atzDEF convert cyanuric acid to carbon dioxide and ammonia, which the cell digests. The atzDEF operon is regulated by atzR, a protein that autoregulates its production in the same region. GlnK is produced under nitrogen limitation conditions and induces expression of atzDEF. This part should be used in conjunction with BBa_M33333, the atzDEF operator region. See related parts BBa_M33331, BBa_M33332, and BBa_M33333. false false _577_ 0 6930 9 Not in stock false We made 4 silent point mutations to make this sequence conform to Assembly Standard 10: 3 for EcoRI and 1 for NotI. We also edited to make TAA the stop codon. This sequence may not work in E. coli. false Frank Li BBa_M33330_sequence 1 ttacgttgcattgtgggtcgtatcagcgagcttgccggcgacgaactccgcaaatgccgccgatgccacaggcagcaccctctcacgcaatgcgaccagtacgacgcgtccggaagccaggctgcgctcgtcgattggaattgcaacctcaccctccgtggaaccagcaccgatctcgatctgaaagctcaccccaccggtttcgcgggcaaaaccgcgcatcatttcgtaggaattactgaccagttgaggccgcggtcgagctgatgatttgtcgaataactcgtccaacacgttgcgcccggagagactgctgtccggtagcgcaagggggtagtccaggcaatctttcagacgcagactcgtacgcttcgcgagcggatggtcggaagcaacgacggcacagatgcgttgccgcgcttgtgcaatgacagtcaaaccgcgcaggtccgggggattgaagaccagcgccaggtcggccgcgaaatccgtcacggcgagaacagcacgctcgcggtccagcacctttacgtcgaaggcaatacctggacgttgggcctggaacgcgtgtattacgcggggcaggaactccggagccagcgcctggctggctgccaatgtcacacggccgcgtttcatgccgctgagatcctggatctgtgactggacttgttccagatcggcattgcggcgacgagcgtaggcaacgaacagttcgcctgccgcagtcaggcgtacaccacgcggcagacgctcgaaaatcggcgtacctaactcgtattccagatcctggacgcggcgatttaccgctgatgcggcgacgtgcagctgttctgcggcggcccgaatggagccgcagcgagccacggcatcgatgtaatgaagaaagcgtaaatgttgcataggtggtcgaagtaaaaaggatggaatagagatggcctgtatcgctgacggccgctgtgcccgcat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z